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CN-122005929-A - Photosynthesis-imitating radio stimulation hydrogel and preparation method and application thereof

CN122005929ACN 122005929 ACN122005929 ACN 122005929ACN-122005929-A

Abstract

The invention discloses photosynthesis-imitating radio stimulation hydrogel, a preparation method and application thereof, the hydrogel is prepared from polyvinylpyrrolidone, pyrrole, protein and sodium copper chlorophyllin as raw materials. The invention constructs a high-conductivity and high-compliance hydrogel biological interface for capturing light energy by simulating the original photochemical reaction in photosynthesis, wherein modified polypyrrole nano particles rapidly diffuse carriers, photocurrent generated by the hydrogel biological interface can effectively induce action potential, accurate regulation and control of nerve excitation and composite muscle action potential are realized, a key basis is provided for regulating electrophysiological signals in the nerve repair process, and the biological interface shows multiple-effect therapeutic action, including instant reconstruction of nerve functions, promotion of nerve repair, inhibition of muscle atrophy and acceleration of healing of epidermis wound.

Inventors

  • DONG XIAOCHEN
  • WANG QIAN
  • Gan Dingli
  • QU XINYU

Assignees

  • 南京工业大学

Dates

Publication Date
20260512
Application Date
20260206

Claims (15)

  1. 1. A method for preparing photosynthesis-imitating radio stimulation hydrogel, which is characterized by comprising the following steps: s1, dissolving polyvinylpyrrolidone and pyrrole in deionized water, stirring for 30 minutes at room temperature, then adding an initiator, continuously keeping stirring for 3 hours at room temperature, centrifuging a product, and washing with acetone/ethanol (1:1) for three times to obtain polypyrrole nano particles; S2, dropwise adding the polypyrrole nanoparticle solution prepared in the step S1 into the L-cysteine solution under stirring, heating the solution to 60-80 ℃ for reaction for 6-8 hours, centrifuging the product, and washing the product with deionized water and ethanol to obtain the L-cysteine modified polypyrrole nanoparticle; S3, dissolving protein in PBS buffer solution, stirring for 4 hours at room temperature, reducing the temperature to 4 ℃ and keeping for 8 hours, centrifuging the product, adjusting to a proper concentration, adding 1M hydrochloric acid solution into the protein solution to adjust the pH to 5.5, stirring for 1 hour at room temperature, heating the solution to 100 ℃ and continuously stirring for 1.5 hours, and immersing the product into an ice water bath to obtain protein nano particles; S4, heating the sodium copper chlorophyllin solution to 60 ℃, dropwise adding the protein nanoparticle solution prepared in the step S3, continuously stirring for 1 hour, cooling to room temperature, centrifuging, and washing with deionized water to obtain a chlorophyll-protein compound; And S5, dissolving gelatin powder in deionized water, heating to 50 ℃ and stirring for 30 minutes, then adding the L-cysteine modified polypyrrole nano particles prepared in the step S2 and the chlorophyll-protein compound prepared in the step S4 in a certain mass ratio under a stirring state, ultrasonically removing bubbles, pouring the homogeneous solution into a polytetrafluoroethylene mould, naturally cooling to form gel, and performing salting-out circulation for a plurality of times by using ammonium sulfate solutions and deionized water with different mass ratios to obtain the hydrogel.
  2. 2. The method for preparing the photosynthesis-imitating radio stimulation hydrogel, which is disclosed in claim 1, is characterized in that in step S1, the mass ratio of pyrrole to polyvinylpyrrolidone is 1:10-1:5, the mass ratio of initiator to polyvinylpyrrolidone is 1:2-1:1, the initiator is ferric trichloride, and the centrifugation speed is 4000 rpm.
  3. 3. The method for preparing the photosynthesis-imitating radio stimulation hydrogel according to claim 2, wherein in the step S1, the mass ratio of the pyrrole to the polyvinylpyrrolidone is 1:10-1.5:10, and the mass ratio of the initiator to the polyvinylpyrrolidone is 3:4.
  4. 4. The method for preparing a photosynthesis-imitating radio stimulation hydrogel according to claim 3, wherein in the step S1, the mass ratio of pyrrole to polyvinylpyrrolidone is 1:10-1.3:10.
  5. 5. The method for preparing the photosynthesis-imitating radio stimulation hydrogel according to claim 1, wherein in the step S2, the mass ratio of the L-cysteine to the polypyrrole nano particles is 1:50-1:150, and the centrifugation speed is 8000 rpm.
  6. 6. The method for preparing a photosynthesis-imitating radio stimulation hydrogel according to claim 5, wherein in the step S2, the mass ratio of the L-cysteine to the polypyrrole nanoparticles is 1:100-1:150.
  7. 7. The method for preparing a photosynthesis-imitating radio stimulation hydrogel according to claim 6, wherein in the step S2, the mass ratio of the L-cysteine to the polypyrrole nanoparticles is 1:100-1:133.
  8. 8. The method for preparing the photosynthesis-imitating radio-stimulated hydrogel according to claim 1, wherein in the step S3, the protein is one of casein, silk protein and bovine serum albumin, the centrifugation speed is 8000 rpm, and the concentration of the protein after being dissolved in PBS buffer solution and being centrifuged is adjusted to 5 mg/mL.
  9. 9. The method for preparing the photosynthesis-imitating radio stimulation hydrogel according to claim 1, wherein in the step S4, the mass ratio of the sodium copper chlorophyllin to the protein nano particles is 1:20-1:50, and the centrifugation speed is 8000 rpm.
  10. 10. The method for preparing photosynthesis-imitating radio stimulation hydrogel according to claim 9, wherein in the step S4, the mass ratio of the sodium copper chlorophyllin to the protein nanoparticles is 1:30-1:40.
  11. 11. The method for preparing photosynthesis-imitating radio stimulation hydrogel according to claim 10, wherein in the step S4, the mass ratio of the sodium copper chlorophyllin to the protein nanoparticles is 1:30-1:33.
  12. 12. The method for preparing the photosynthesis-imitating radio-stimulated hydrogel according to claim 1, wherein in the step S5, the mass ratio of the gelatin powder dissolved in deionized water is 15-wt%, the mass ratio of the L-cysteine modified polypyrrole nanoparticles to the chlorophyll-protein complex is one of 8:1, 5:1, 4:1, 3:1, 2:1, 1:1 and 1:2, the mass ratio of the ammonium sulfate solution is 1-wt%, 5-wt%, 10-wt%, 20-wt% and 30-wt%, the cyclic salting-out times are 1-5, the ultrasonic power is 100-W, each working time is 3 seconds, the interval is 1 second, and the centrifugation speed is 8000 rpm.
  13. 13. A photosynthetically mimicking radio-stimulated hydrogel prepared by the method of any one of claims 1-12.
  14. 14. A photosynthetically mimicking radio-stimulated hydrogel biological interface, wherein the biological interface is stimulated by laser light to photocurrent after implantation of the hydrogel of claim 13 into biological tissue.
  15. 15. Use of a photosynthesis-mimicking radio-stimulated hydrogel biological interface according to claim 14, wherein said biological interface is used for the reconstruction of damaged sciatic nerve function, modulation of nerve electrical signals, electrical stimulation repair of sciatic nerve, electrical stimulation repair of epidermal wounds.

Description

Photosynthesis-imitating radio stimulation hydrogel and preparation method and application thereof Technical Field The invention belongs to the technical field of biological materials, and particularly relates to photosynthesis-imitating radio stimulation hydrogel and a preparation method and application thereof. Background The nerve regulation technology realizes selective regulation and control on nerve tissues by applying chemical or physical stimulation, and has great application prospect in diagnosis and treatment of nervous system diseases. This ability to regulate the neural loop is essentially based on modulation of the ionic current that underlies all electrophysiological activity. Among the physical neuromodulation means, electrical stimulation therapy, which is a representative technique, stands out by virtue of its excellent biocompatibility advantages. However, in consideration of the diversity of damaged nerves, the wide range of signal amplitudes (from microvolts to millivolts) and the variability of detection sites, the stimulation performance and recording fidelity of the conventional rigid electrode are significantly affected due to the high modulus and limited stretchability. In addition, the existing electrical stimulation equipment is highly dependent on invasive electrode implantation, so that the risk of infection is high, and the electrode is required to be taken out through secondary operation. In recent years, implantable micro devices capable of generating electrical stimulation have been widely studied and applied in the field of tissue regeneration in order to enhance therapeutic efficacy and reduce invasive damage. Researchers are working to optimize stimulus-responsive flexible biological interfaces to achieve efficient bioadhesion, as well as long-term, high-fidelity signal acquisition. However, such devices have a large interface contact area, which often results in low charge injection efficiency, thereby compromising the accuracy and performance of neuromodulation. Therefore, developing innovative neuromodulation strategies that combine high energy conversion efficiency, convenient operating characteristics, and biosafety remains a central challenge in the current field. Disclosure of Invention The invention aims to provide photosynthesis-imitating radio stimulation hydrogel, a preparation method and application thereof, and a biological interface based on the hydrogel, wherein the hydrogel biological interface has the advantages of high conductivity, high compliance, high biocompatibility and the like, and can effectively widen the application of the hydrogel biological interface in non-invasive nerve modulation and tissue engineering. The invention aims at realizing the following technical scheme: A method for preparing photosynthesis-imitating radio stimulation hydrogel, which comprises the following steps: s1, dissolving polyvinylpyrrolidone and pyrrole in deionized water, stirring for 30 minutes at room temperature, then adding an initiator, continuously keeping stirring for 3 hours at room temperature, centrifuging a product, and washing with acetone/ethanol (1:1) for three times to obtain polypyrrole nano particles; S2, dropwise adding the polypyrrole nanoparticle solution prepared in the step S1 into the L-cysteine solution under stirring, heating the solution to 60-80 ℃ for reaction for 6-8 hours, centrifuging the product, and washing the product with deionized water and ethanol to obtain the L-cysteine modified polypyrrole nanoparticle; S3, dissolving protein in PBS buffer solution, stirring for 4 hours at room temperature, reducing the temperature to 4 ℃ and keeping for 8 hours, centrifuging the product, adjusting to a proper concentration, adding 1M hydrochloric acid solution into the protein solution to adjust the pH to 5.5, stirring for 1 hour at room temperature, heating the solution to 100 ℃ and continuously stirring for 1.5 hours, and immersing the product into an ice water bath to obtain protein nano particles; S4, heating the sodium copper chlorophyllin solution to 60 ℃, dropwise adding the protein nanoparticle solution prepared in the step S3, continuously stirring for 1 hour, cooling to room temperature, centrifuging, and washing with deionized water to obtain a chlorophyll-protein compound; And S5, dissolving gelatin powder in deionized water, heating to 50 ℃ and stirring for 30 minutes, then adding the L-cysteine modified polypyrrole nano particles prepared in the step S2 and the chlorophyll-protein compound prepared in the step S4 in a certain mass ratio under a stirring state, ultrasonically removing bubbles, pouring the homogeneous solution into a polytetrafluoroethylene mould, naturally cooling to form gel, and performing salting-out circulation for a plurality of times by using ammonium sulfate solutions and deionized water with different mass ratios to obtain the hydrogel. Preferably, in the step S1, the mass ratio of the pyrrole to the polyvin