CN-122005933-A - Preparation and application of nano enzyme implantation material

CN122005933ACN 122005933 ACN122005933 ACN 122005933ACN-122005933-A

Abstract

The invention discloses a preparation method and application of a nano enzyme implant material, which belong to the technical field of medicines, wherein the preparation method of the nano enzyme implant material comprises the following steps of S1, providing a biocompatible bracket, cleaning and drying the bracket, S2, placing the bracket treated in the step S1 into an ion implanter, taking one or more elements selected from carbon, magnesium, aluminum, titanium, chromium, iron, cobalt, nickel, copper, silver, platinum, vanadium and manganese as an implant element source, and performing ion implantation at the implant energy of 20-140keV and the implant dose of 1X 10 12 -5×10 17 ions/cm 2 , so as to form a nano enzyme modification layer on the surface of the bracket, thereby obtaining the nano enzyme implant material. The invention develops the nano enzyme implant material which can be produced in a large scale and regulated freely, shows excellent biocatalysis activity similar to natural enzymes, has long-term sustainable biocatalysis activity and good catalytic stability, can inhibit inflammation when being applied to diseases, avoids collagen adhesion, and is beneficial to body recovery.

Inventors

ZHANG XIAODONG
PEI JIAHUI
Zhao Ruoli
WANG YILI
CHEN KE
WANG HAO

Assignees

天津大学

Dates

Publication Date: 20260512
Application Date: 20251212

Claims (10)

1. The preparation method of the nano enzyme implant material is characterized by comprising the following steps: S1, providing a biocompatible stent, and cleaning and drying the stent; S2, placing the bracket treated in the step S1 in an ion implanter, taking one or more elements selected from carbon, magnesium, aluminum, titanium, chromium, iron, cobalt, nickel, copper, silver, platinum, vanadium and manganese as implantation element sources, and performing ion implantation at implantation energy of 20-140keV and implantation dosage of 1X 10 12 -5×10 17 ions/cm 2 , so that a nano enzyme modification layer is formed on the surface of the bracket, and the nano enzyme implantation material is obtained.
2. The method for preparing a nano-enzyme implant material according to claim 1, wherein in S1, the washing includes ultrasonic washing of the stent sequentially using acetone and absolute ethyl alcohol.
3. The method for preparing a nano enzyme implant material according to claim 1, wherein in the step S2, the implant element source further comprises one of niobium, molybdenum, hafnium, tungsten, bismuth, uranium, lithium, calcium, scandium, silicon, zinc, germanium, palladium, strontium, yttrium, zirconium, cadmium, indium, tin, antimony, barium, lanthanum, cerium, praseodymium, samarium, neodymium, gadolinium, dysprosium, holmium, erbium, thulium, ytterbium, tantalum, iridium, gold, palladium, and thorium, and the single metal ion implantation is performed, or two of the metals are sequentially or simultaneously implanted, so as to form a bimetal nano enzyme modification layer.
4. The method for preparing a nano-enzyme implant material according to claim 1, wherein in S1, the biocompatible scaffold is selected from one of titanium alloy, cobalt alloy, niobium alloy, zirconium alloy, stainless steel, alumina, medical carbon, hydroxyapatite, bone cement, bioactive glass, polyether ether ketone, polylactic acid, polyorthoester, polyanhydride, polyphosphoester, polyglycolic acid, and polycaprolactone.
5. A nanoenzyme implant material, characterized in that it is produced by the method for producing a nanoenzyme implant material according to any one of claims 1 to 4.
6. The nano-enzyme implant material according to claim 5, wherein the implant element is dispersed in an atomic form within a depth range of 10-20nm below the stent surface.
7. The nano-enzyme implant material according to claim 5, wherein the nano-enzyme implant material has one or more enzyme-like catalytic activities selected from the group consisting of antioxidant activity, peroxidase-like activity, catalase-like activity, NADH oxidase-like activity.
8. Use of a nanoenzyme implant material of any one of claims 5 to 7 in the manufacture of a medical device for the treatment or prevention of implant-related inflammation, fibrosis or restenosis.
9. The use according to claim 8, wherein the medical device is for treating or preventing restenosis following vascular injury, or for promoting bone defect repair.
10. The use according to claim 8 or 9, wherein the nanoenzyme implant material is made in the form of a bone plate, bone nail, bone needle, bone rod, spinal column internal fixation device, heart or tissue repair material, intraocular filling material, intrauterine device, nerve patch, artificial esophagus, artificial blood vessel, artificial vertebral body, artificial joint, artificial urethra, artificial valve, artificial kidney, artificial breast, artificial skull, artificial jawbone, artificial heart, artificial tendon, artificial cochlea, artificial anus sealer, vascular stent, prostate stent, biliary stent, esophageal stent.

Description

Preparation and application of nano enzyme implantation material Technical Field The invention belongs to the technical field of medicines, and particularly relates to preparation and application of a nano enzyme implantation material. Background In modern medical treatment, implantable materials are widely used in a variety of settings such as tissue repair, drug delivery, medical device support, etc., such as artificial joints, cardiovascular stents, and bone repair materials. However, these implant materials, when introduced into the body, are recognized as foreign bodies by the host system and cause a series of local or systemic adverse reactions, mainly including problems of acute inflammation, fibrosis, coagulation, infection, etc. Fibrinogen adsorbed on the surface of the implant material is an important determinant of an acute inflammatory reaction, and eventually collagen tends to deposit around the material to form a fibrotic envelope, isolating the implant from host tissue, and even causing malfunction or rejection of the device. The implant material is therefore generally inert and non-toxic. Surface engineering is an important means of regulating the interaction of implants with the host, aiming at reducing oxidative stress and inhibiting chronic inflammation, improving the long-term safety of the material. However, the prior art often faces the problems of large chemical dosage, high energy consumption, high cost and the like, and limits the wide application of the prior art. Polyethylene glycol (PEG) is used as a common bio-inert coating material, and although the compatibility of the material is improved to a certain extent, the polyethylene glycol (PEG) is easy to oxidize and degrade under physiological conditions, and has limited inhibition effect on chronic inflammation, so that the requirement on biostability required by long-term implantation is difficult to meet. The nano-enzyme as a novel artificial enzyme with advantages of adjustable catalytic activity, multifunction, high stability and the like has great potential in the fields of disease diagnosis and treatment. However, its catalytic activity is generally lower than that of natural enzymes, and current methods of modifying it to implant materials by chemical synthesis face high costs, long time consumption and the like, and is difficult to realize large-scale application. Therefore, development of an implant material having both long-term sustainable bioactivity and large-scale preparation feasibility to achieve effective inhibition of acute and chronic inflammation has become an urgent need in the art. The new generation nano enzyme implant material is expected to realize long-acting anti-inflammatory, antioxidant and antibacterial functions in vivo through accurate catalytic design, not only opens up a road for improving the performance of long-term implants such as cardiovascular stents, bone repair materials and the like, but also is expected to promote the intelligent evolution of implanted medical equipment Disclosure of Invention The primary aim of the invention is to overcome the defects of the prior art and provide a preparation method of the nano enzyme implant material, which has simple and convenient process and strong controllability and is suitable for large-scale production. The invention also aims to provide the nano enzyme implant material prepared by the method, and the surface of the material has stable and efficient nano enzyme catalytic activity. A third object of the present invention is to provide the use of the above nanoenzyme implant material for preparing a medical device for anti-inflammatory, antioxidant and tissue repair promotion. In order to achieve the above object, a specific embodiment of the present invention provides the following technical solution: The preparation method of the nano enzyme implant material comprises the following steps: S1, providing a biocompatible stent, and cleaning and drying the stent; S2, placing the bracket treated in the step S1 in an ion implanter, taking one or more elements selected from carbon, magnesium, aluminum, titanium, chromium, iron, cobalt, nickel, copper, silver, platinum, vanadium and manganese as implantation element sources, and performing ion implantation at implantation energy of 20-140keV and implantation dosage of 1X 10 12-5×1017ions/cm2, so that a nano enzyme modification layer is formed on the surface of the bracket, and the nano enzyme implantation material is obtained. In one or more embodiments of the present invention, in S1, the washing includes ultrasonically washing the stent using acetone and absolute ethanol in sequence. In one or more embodiments of the present invention, in the S2, the implantation element source further includes one of niobium, molybdenum, hafnium, tungsten, bismuth, uranium, lithium, calcium, scandium, silicon, zinc, germanium, palladium, strontium, yttrium, zirconium, cadmium, indium, tin, antimony, barium, lanthan