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CN-122005950-A - Nanofiber scaffold and preparation method thereof

CN122005950ACN 122005950 ACN122005950 ACN 122005950ACN-122005950-A

Abstract

A nanofiber scaffold and a preparation method thereof belong to the technical field of biomedical materials. The preparation method of the nanofiber scaffold comprises the steps of taking two parts of nanofiber membranes, respectively carrying out glutaraldehyde steam crosslinking treatment to obtain a crosslinked first nanofiber material, carrying out thermal crosslinking treatment to obtain a crosslinked second nanofiber material, uniformly dispersing the first nanofiber material and the second nanofiber material in a freeze-drying solvent according to a mass ratio of 1:0.2-5 to obtain nanofiber dispersion liquid, and carrying out freeze-drying and vacuum drying on the nanofiber dispersion liquid to obtain the nanofiber scaffold, wherein the freeze-drying solvent in the dispersion liquid of the first nanofiber material always contains tertiary butanol. The nanofiber scaffold prepared by the method has good structural stability and appearance quality.

Inventors

  • Zha Fangwen
  • LIN QINGZHU

Assignees

  • 惠州华阳医疗器械有限公司

Dates

Publication Date
20260512
Application Date
20241108

Claims (10)

  1. 1. A method of preparing a nanofiber scaffold comprising: taking two parts of nanofiber membranes, respectively performing glutaraldehyde steam crosslinking treatment to obtain a crosslinked first nanofiber material, and performing thermal crosslinking treatment to obtain a crosslinked second nanofiber material; The preparation method comprises the steps of uniformly dispersing a first nanofiber material and a second nanofiber material in a freeze-drying solvent according to a mass ratio of 1:0.2-5 to obtain nanofiber dispersion liquid, freeze-drying the nanofiber dispersion liquid, and vacuum-drying to obtain the nanofiber scaffold, wherein the freeze-drying solvent in the dispersion liquid of the first nanofiber material always contains tertiary butanol.
  2. 2. The method of claim 1, wherein the lyophilization solvent in the nanofiber dispersion further comprises water.
  3. 3. The method of preparing according to claim 1, wherein the first nanofiber material and the second nanofiber material are homogeneously dispersed in a lyophilization solvent, comprising: Homogeneously dispersing the first nanofiber material in a freeze-drying solvent containing tertiary butanol to obtain a pre-dispersion liquid; homogeneously dispersing the second nanofiber material in the pre-dispersion to form the nanofiber dispersion; optionally, the ratio of the total mass of the first nanofiber material and the second nanofiber material to the mass of the freeze-drying solvent is 1:50-100.
  4. 4. The method of claim 3, wherein the first nanofiber material is homogeneously dispersed in an aqueous t-butanol solution to obtain the pre-dispersion; optionally, in the tertiary butanol aqueous solution, the mass ratio of the tertiary butanol to the water is 1-5:1.
  5. 5. The method of claim 3, wherein the first nanofiber material is homogeneously dispersed in t-butanol to obtain the pre-dispersion; adding water and the second nanofiber material into the pre-dispersion liquid, and performing uniform dispersion to form the nanofiber dispersion liquid; optionally, the mass ratio of the tertiary butanol to the water is 1-5:1.
  6. 6. The preparation method according to claim 1, wherein the glutaraldehyde steam crosslinking treatment has a crosslinking temperature of 50-80 ℃ and a crosslinking time of 10-18h; And/or the crosslinking temperature of the thermal crosslinking treatment is 120-180 ℃, the crosslinking time is 1-4h, and optionally, the thermal crosslinking time is 2h.
  7. 7. The method of preparing a nanofiber membrane according to claim 1, comprising: carrying out electrostatic spinning on spinning solution containing high polymer materials to obtain the nanofiber membrane; optionally, the polymer material is selected from hydrophilic polymer materials; optionally, the polymer material is selected from polyvinyl alcohol; optionally, the spinning solution further contains a surfactant; Optionally, the surfactant is selected from sodium dodecyl sulfate and/or polydimethylsiloxane; Optionally, the parameters of the electrostatic spinning comprise a spinning voltage of 15-45kV, a spinning solution flow rate of 0.2-1ml/h, a receiving roller rotating speed of 600-1000r/min, a temperature of 20-30 ℃ and a humidity of 30-60%.
  8. 8. The method according to claim 1, wherein the vacuum drying is performed at a temperature of 100 to 150 ℃ for a time of 10 to 18 hours.
  9. 9. The method according to claim 1, wherein the freeze-drying is carried out at a temperature of-50 to 30 ℃ for a period of 18 to 35 hours.
  10. 10. A nanofiber scaffold produced according to the production process of any one of claims 1-9.

Description

Nanofiber scaffold and preparation method thereof Technical Field The application relates to the technical field of biomedical materials, in particular to a nanofiber scaffold and a preparation method thereof. Background At present, a nanofiber scaffold with a three-dimensional structure is widely applied to the field of biomedical materials. Generally, the three-dimensional structure nanofiber scaffold is prepared by carrying out homogenizing and crushing treatment on an electrostatic spinning nanofiber membrane, and then carrying out freeze drying and vacuum drying. However, the nanofiber scaffold prepared at present is easy to have the problems of loose structure and rough appearance. Disclosure of Invention Based on the defects, the application provides a nanofiber scaffold and a preparation method thereof, so as to improve the quality of the nanofiber scaffold. The application is realized in the following way: in a first aspect, examples of the present application provide a method of preparing a nanofiber scaffold comprising: Taking two parts of nanofiber membranes, respectively performing glutaraldehyde steam crosslinking treatment to obtain a crosslinked first nanofiber material and performing thermal crosslinking treatment to obtain a crosslinked second nanofiber material, uniformly dispersing the first nanofiber material and the second nanofiber material in a freeze-drying solvent according to a mass ratio of 1:0.2-5 to obtain nanofiber dispersion liquid, performing freeze-drying and vacuum drying on the nanofiber dispersion liquid to obtain a nanofiber bracket, wherein the freeze-drying solvent in the dispersion liquid of the first nanofiber material always contains tertiary butanol. In the implementation process, the glutaraldehyde steam-crosslinked first nanofiber material and the thermally crosslinked second nanofiber material are uniformly dispersed in a freeze-drying solvent to form nanofiber dispersion liquid, and then the nanofiber dispersion liquid is subjected to freeze drying and vacuum drying, so that the nanofiber scaffold with a three-dimensional structure can be obtained. By utilizing the mutual matching of the first nanofiber material after glutaraldehyde steam crosslinking and the second nanofiber material after thermal crosslinking, the structural stability and the appearance quality of the nanofiber scaffold can be improved. In addition, as the glutaraldehyde steam-crosslinked first nanofiber material is always dispersed in the freeze-drying solvent containing tertiary butanol, the mixing uniformity of nanofiber dispersion liquid can be improved, and the pore structure and the structural stability of the nanofiber scaffold can be improved. With reference to the first aspect, in an alternative embodiment of the application, the lyophilization solvent in the nanofiber dispersion further comprises water. In the implementation process, the freeze-drying solvent in the nanofiber dispersion liquid contains water, so that the pore structure of the nanofiber scaffold can be adjusted, the environmental protection of the nanofiber scaffold preparation process is improved, and the nanofiber scaffold preparation method is more suitable for biomedical materials. With reference to the first aspect, in an alternative embodiment of the present application, the first nanofiber material and the second nanofiber material are homogeneously dispersed in a lyophilization solvent, comprising: Homogeneously dispersing the first nanofiber material in a freeze-drying solvent containing tertiary butanol to obtain a pre-dispersion liquid; and (3) uniformly dispersing the second nanofiber material in the pre-dispersion liquid to form nanofiber dispersion liquid. Optionally, the ratio of the total mass of the first nanofiber material and the second nanofiber material to the mass of the freeze-drying solvent is 1:50-100. In the implementation process, the first nanofiber is firstly homogenously dispersed into the freeze-drying solvent containing tertiary butanol, so that the first nanofiber material can be uniformly dispersed, and the uniformly dispersed pre-dispersion liquid can be obtained. And then, the second nanofiber material is homogeneously dispersed into the pre-dispersion liquid, so that the dispersion uniformity of the obtained nanofiber dispersion liquid can be improved, the pore structure and the appearance quality of the nanofiber scaffold can be improved, and the structural stability can be improved. With reference to the first aspect, in an alternative embodiment of the present application, the first nanofiber material is homogeneously dispersed in an aqueous t-butanol solution to obtain a pre-dispersion. Optionally, in the tertiary butanol aqueous solution, the mass ratio of tertiary butanol to water is 1-5:1. In the implementation process, the first nanofiber material subjected to glutaraldehyde steam crosslinking is uniformly dispersed into the tertiary butanol aqueous solution, even if the