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CN-122005952-A - Tubular support with electrostatic spinning core-shell structure and preparation method and application thereof

CN122005952ACN 122005952 ACN122005952 ACN 122005952ACN-122005952-A

Abstract

The invention relates to an electrostatic spinning core-shell structure tubular support, which comprises a core and a shell, wherein the core comprises polyvinylidene fluoride and cholestyramine, the shell comprises polyvinyl butyral and adenosylmethionine, and the shell is wrapped on the surface of the core. The preparation method of the electrostatic spinning core-shell structure tubular stent comprises the following steps of preparing a core solution, preparing a shell solution and preparing the core-shell structure tubular stent. The core layer of the tubular scaffold with the electrostatic spinning core-shell structure loads cholestyramine to be chelated with bile acid in the bile duct, so that absorption of human body to bile acid is reduced, the adenomethionine loaded on the outer shell layer regulates immune environment, oxidation resistance of liver cells is enhanced, the environment of the bile duct is comprehensively improved through synergistic effect, treatment effect is improved, the core-shell structure solves time-space sequential release of medicines, and medicine delivery efficiency and restoration effect are enhanced, so that the tubular scaffold can be used for bile duct restoration products.

Inventors

  • XIANG YANG
  • GAO YUANHUI
  • LEI ZHONGWEN

Assignees

  • 海口市人民医院(中南大学湘雅医学院附属海口医院、海口市人民医院医疗集团总院)

Dates

Publication Date
20260512
Application Date
20260211

Claims (10)

  1. 1. The tubular scaffold is characterized by comprising a core and a shell, wherein the core comprises polyvinylidene fluoride and cholestyramine, the shell comprises polyvinyl butyral and adenosylmethionine, and the shell is wrapped on the surface of the core.
  2. 2. The method for preparing the tubular scaffold with the electrostatic spinning core-shell structure according to claim 1, comprising the following steps: preparing a nuclear solution, namely dissolving polyvinylidene fluoride in an organic solvent, cooling, adding cholestyramine, stirring, and carrying out vacuum defoaming to obtain the nuclear solution; Preparing a shell solution, namely dissolving polyvinyl butyral in an organic solvent, adding adenosylmethionine, performing ultrasonic dispersion, and performing vacuum defoaming to obtain the shell solution; And (3) preparing the tubular stent with the core-shell structure, namely carrying out electrostatic spinning by taking the core solution and the shell solution as raw materials to obtain the tubular stent, and carrying out vacuum drying to obtain the tubular stent with the electrostatic spinning core-shell structure.
  3. 3. The method according to claim 2, wherein the organic solvent comprises methylene chloride or N, N-dimethylformamide in the preparation of the core solution, and wherein the organic solvent comprises absolute ethanol in the preparation of the shell solution.
  4. 4. The method according to claim 2, wherein the stirring is magnetic stirring, the stirring time is 1-3 hours, and the vacuum defoaming time is 20-40 minutes.
  5. 5. The method according to claim 2, wherein the ultrasonic dispersion time is 20 to 40min and the vacuum defoaming time is 20 to 40min in the preparation of the shell solution.
  6. 6. The preparation method of the core-shell tubular support according to claim 2, wherein in the preparation of the core-shell tubular support, the time of electrostatic spinning is 2-10 h, the vacuum drying temperature is 38-45 ℃ and the time is 20-26 h.
  7. 7. The method according to claim 2, wherein in the preparation of the tubular scaffold of core-shell structure, the electrospinning is achieved by a coaxial electrospinning device, and the electrospinning step comprises injecting the core solution into an inner syringe, injecting the shell solution into an outer syringe, installing into the coaxial electrospinning device, and spinning under working conditions; The working conditions comprise 10-20 kV voltage, 10-20 cm receiving distance, 0.1-0.5 mL/h nuclear layer flow rate, 0.3-1.5 mL/h shell flow rate and 3-10 mm receiving roller diameter.
  8. 8. The method according to any one of claims 2 to 7, wherein in the preparation of the core solution, the mass fraction concentration of the polyvinylidene fluoride after dissolution is 5 to 20%, the mass fraction concentration of the cholestyramine after dissolution before adding the polyvinylidene fluoride solution is 0.1 to 1%, and the mass ratio of the polyvinylidene fluoride to the cholestyramine is (10 to 150): 1; in the preparation of the shell solution, the mass fraction concentration of the dissolved polyvinyl butyral is 8% -20%, the mass fraction concentration of the dissolved adenosyl methionine before the polyvinyl butyral solution is added is 0.1% -5%, and the mass ratio of the polyvinyl butyral to the adenosyl methionine in the shell solution is (1) - (100): 1.
  9. 9. Use of an electrospun core-shell structured tubular scaffold according to claim 1 or obtained by the preparation method according to claims 2-8 for the preparation of a product for repairing bile duct injury.
  10. 10. A product for repairing bile duct injury, comprising the tubular scaffold with an electrostatic spinning core-shell structure according to claim 1 or the tubular scaffold with an electrostatic spinning core-shell structure obtained by the preparation method according to claims 2-8.

Description

Tubular support with electrostatic spinning core-shell structure and preparation method and application thereof Technical Field The invention relates to the technical field of biomedicine, in particular to an electrostatic spinning core-shell structure tubular support, and a preparation method and application thereof. Background Bile duct diseases, including bile duct stenosis, obstruction, inflammation, bile duct cancer, and the like, are clinically common digestive system diseases. These diseases often lead to obstructive jaundice, liver function damage and infection, severely affecting the quality of life and survival of the patient. Bile duct cancer is often diagnosed in advanced stages as an invasive tumor, surgery is the only potential curative treatment, but the recurrence rate is high, and the estimated new case rate of 2025 intrahepatic bile duct cancer is about 9.4/10 ten thousand. For patients who cannot be resected or do not want to operate, biliary stent placement has become the first palliative treatment means, and can effectively relieve obstruction, improve jaundice and prolong survival time. The existing biliary tract stent mainly comprises a plastic stent, a self-expanding metal stent (SEMS), a covered metal stent, an anti-reflux stent, a drug eluting stent, a radioactive stent and the like. The plastic stent has low cost and is easy to put in, but is easy to block and shift, and multiple Endoscopic Retrograde Cholangiopancreatography (ERCP) interventions are needed, so that the risk and the hospitalization time of patients are increased. The metal stent has longer patency period (median lifetime >3 months) and is suitable for patients with longer expected lifetime, but has the problems of in-tumor growth, restenosis, stent failure and the like. The stent graft is intended to prevent tumor embedding, but several studies have shown that its long-term patency has no significant advantage and may increase the risk of displacement. Anti-reflux stents and drug eluting stents, while partially improving patency and survival, have small clinical test sample size, structural advantages are not fully validated, and complications such as late cholangitis still occur up to 36.7%. The radioactive stent shows positive effects in combination with chemotherapy, but the safety data are limited, and core problems such as stent blockage, displacement and tumor growth are not thoroughly solved. In addition, the existing self-falling bracket can avoid secondary bracket taking, but is only suitable for short-term treatment, and has insufficient mechanical property and biocompatibility. As an emerging nanofiber preparation method, the electrostatic spinning technology is widely applied to the field of tissue engineering, can be used for preparing a fiber scaffold with high surface area, high porosity and a bionic structure, and promotes cell attachment, proliferation and tissue regeneration. The core-shell structure nanofiber is realized through coaxial electrostatic spinning, can be loaded with drugs, growth factors or bioactive molecules, realizes controllable release, such as pulse release or sustained release, and is suitable for drug delivery and local treatment. The technology has proved effective in myocardial, skeletal, vascular and skin tissue engineering, but the research applied to bile duct scaffolds is still under exploration, and a special core-shell structure design is lacking to solve bile duct specificity problems, such as anti-inflammatory, anti-fibrosis and promotion of epithelial regeneration. The limitations of the existing bile duct stent are poor biocompatibility, uncontrollable degradation rate, uneven drug release, and inability to effectively promote tissue regeneration, resulting in high restenosis rate and limited lifetime. Therefore, there is a need to develop a novel stent that can combine the nanostructure advantages of electrospinning and the drug controlled release function of core-shell design, providing better mechanical support, biodegradability and regeneration promoting effect to improve the therapeutic outcome of bile duct diseases. In view of the above, biliary stents require multi-target interventions that compromise anti-inflammatory, anti-fibrotic and promote epithelial regeneration. Based on core-shell structure electrostatic spinning technology, cholestyramine (core layer, chelated bile acid) and adenosyl methionine (outer shell layer, inflammation regulation and oxidation resistance enhancement) are functionally integrated, so that the core-shell structure can be utilized to realize function time sequence release, and bile duct environment can be improved through multicomponent synergistic effect. Disclosure of Invention In order to overcome the defects of the prior art, the invention provides an electrostatic spinning core-shell structure tubular support, which comprises a core and a shell, wherein the core comprises polyvinylidene fluoride (PVDF) and cholestyramine