CN-122005958-A - Full-suture anchor with bioactive peptide coating and preparation method thereof

Abstract

The invention discloses a full suture anchor with a bioactive peptide coating and a preparation method thereof. The full-suture anchor with the bioactive peptide coating comprises an anchor-like line segment and a suture, wherein the anchor-like line segment is of a woven structure, a polypeptide coating capable of specifically activating osteoblast activity is attached to the surface of the anchor-like line segment, the suture penetrates through the anchor-like line segment in a staggered penetrating mode, and the anchor-like line segment contracts or stretches in a sliding mode relative to the suture. According to the invention, the polypeptide coating with the capability of specifically activating osteoblast activity is added on the surface of the anchor-like line segment, and the osteoblast activity is specifically activated through the polypeptide, so that bone repair and tissue bridging of a tendon bone interface are promoted, and compared with the traditional full-suture anchor, the full-suture anchor has the capability of promoting tendon bone healing, so that the mechanical strength of the tendon and bone surfaces after suturing is increased, the risk of fracture after tendon operation is reduced, and the recovery of limb functions is promoted.

Inventors

• LIU YANG
• XU ZHONGSHI
• PAN YU
• XU ZHEN
• Dai Bingyang
• CHEN PEIXUAN
• MA WEI
• ZOU SUYING
• XU JIAN
• YU YANGYI

Assignees

• 深圳市人民医院

Dates

Publication Date

20260512

Application Date

20260212

Claims (10)

1. 1. A full-suture anchor with a bioactive peptide coating is characterized by comprising an anchor-like line segment and a suture, wherein the anchor-like line segment is of a woven structure, a polypeptide coating capable of specifically activating osteoblast activity is attached to the surface of the anchor-like line segment, the suture penetrates through the anchor-like line segment in a staggered penetrating mode, and the anchor-like line segment contracts or stretches in a sliding mode relative to the suture.
2. 2. The full suture anchor with bioactive peptide coating of claim 1, wherein the polypeptide of the polypeptide coating has an amino acid sequence of K-DFQTWSFLYVEN or DFQTWSFLYVEN.
3. 3. A full suture anchor having a bioactive peptide coating as claimed in claim 2, wherein said polypeptide is attached to the surface of said anchor-like wire segment by dopamine means.
4. 4. A full-suture anchor with a bioactive peptide coating as claimed in any one of claims 1-3, wherein said anchor-like thread segments are of polyethylene terephthalate or ultra-high molecular weight polyethylene.
5. 5. A full suture anchor having a bioactive peptide coating as claimed in any one of claims 1 to 3, wherein the suture is of polyethylene terephthalate or ultra high molecular weight polyethylene.
6. 6. The full-suture anchor with bioactive peptide coating as claimed in claim 5, wherein said anchor-like thread segment is a solid woven structure woven by a plurality of strands of suture, and has a length of 10-15mm and a diameter of 0.8-1.2mm.
7. 7. A method of preparing a full suture anchor having a bioactive peptide coating for preparing a full suture anchor having an bioactive peptide coating as claimed in any one of claims 1 to 6, comprising the steps of: S1, preparing an original anchor line segment with the surface not attached with polypeptide; s2, cleaning the original anchor line segment to remove grease; S3, immersing the cleaned original anchor line segment into Tris buffer solution containing dopamine for oscillation treatment to obtain an anchor line segment with a polydopamine deposit layer formed on the surface; S4, flushing an anchor line segment with a polydopamine deposition layer formed on the surface by adopting a PBS solution to remove Tris buffer solution residues; s5, immersing the anchor line segment with the polydopamine deposition layer formed on the surface into PBS solution containing polypeptide for oscillation treatment, so that the polypeptide is coupled to the surface of the anchor line segment through the polydopamine deposition layer to form a polypeptide coating, wherein the amino acid sequence of the polypeptide is K-DFQTWSFLYVEN or DFQTWSFLYVEN; And S6, cleaning and drying the anchor line segments with the polypeptide coating on the surfaces, and penetrating the suture lines on the anchor line segments in a staggered and penetrating mode to obtain the full-suture anchor.
8. 8. The method of claim 7, wherein in step S2, the original anchor-like line segment is ultrasonically cleaned with ethanol.
9. 9. The method for preparing a full-suture anchor with a bioactive peptide coating as claimed in claim 7, wherein in the step S3, the concentration of the Tris buffer is 8-12mM, the pH value is 8.0-9.0, the concentration of the dopamine is 1.5-2.5mg/mL, and the shaking treatment time is 2-4 hours.
10. 10. The method of claim 7, wherein in the step S5, the concentration of the polypeptide in the PBS solution is 0.1-1.0 mg/mL, the pH value is 7.5-8.5, and the time of the shaking treatment is 10-14 hours.

Description

Full-suture anchor with bioactive peptide coating and preparation method thereof Technical Field The invention relates to the technical field of medical instruments, in particular to a full-suture anchor with a bioactive peptide coating and a preparation method thereof. Background Full-suture anchors are critical implants used in the orthopedic and sports medical fields to repair soft tissue injuries such as rotator cuff, ligament and tendon, and operate on the principle of implanting the anchoring portion (typically a section of a deformable cinched suture) of the anchor into bone and re-securing the torn tendon or ligament to the bone surface by the penetrated suture. However, the conventional full-suture anchor can only provide mechanical fixation, the biological healing degree of the tendon and the tendon in the attachment area of the bone surface (tendon bone juncture area) after suturing is poor, and the tendon are easy to break again after a certain proportion of repair operations by only virtue of the mechanical fixation of the full-suture anchor. Disclosure of Invention The technical problem to be solved by the invention is to provide a full suture anchor with a bioactive peptide coating and a preparation method thereof, wherein the specific peptide coating is introduced to specifically activate osteoblast activity and promote bone repair and tissue bridging at a tendon bone interface, so that the mechanical strength of the tendon and bone surfaces after suturing is increased, and the risk of fracture after tendon operation is reduced. In order to solve the technical problems, the invention adopts the following technical scheme: The invention provides a full-suture anchor with a bioactive peptide coating, which comprises an anchor-like line segment and a suture, wherein the anchor-like line segment is of a woven structure, a polypeptide coating capable of specifically activating osteoblast activity is attached to the surface of the anchor-like line segment, the suture penetrates through the anchor-like line segment in a staggered penetrating mode, and the anchor-like line segment can shrink or stretch in a sliding mode relative to the suture. Preferably, the amino acid sequence of the polypeptide coating is K-DFQTWSFLYVEN or DFQTWSFLYVEN. Preferably, the polypeptide is attached to the surface of the anchor-like segment by dopamine means. Preferably, the anchor line section is made of polyethylene terephthalate or ultra-high molecular weight polyethylene. Preferably, the suture is made of polyethylene terephthalate or ultra-high molecular weight polyethylene. Preferably, the anchor line segment is a solid knitting structure formed by knitting a plurality of stitches, the length of the anchor line segment is 10-15mm, and the diameter of the anchor line segment is 0.8-1.2mm. The second aspect of the invention provides a method for preparing a full-suture anchor with a bioactive peptide coating, which is used for preparing the full-suture anchor with the bioactive peptide coating, and comprises the following steps of preparing an original anchor-like line segment with no polypeptide attached on the surface; the method comprises the steps of cleaning an original anchor line segment, removing grease, immersing the cleaned original anchor line segment in Tris buffer solution containing dopamine for vibration treatment to obtain an anchor line segment with a polydopamine deposition layer formed on the surface, flushing the anchor line segment with the polydopamine deposition layer formed on the surface by adopting PBS solution to remove the residual Tris buffer solution, immersing the anchor line segment with the polydopamine deposition layer formed on the surface in PBS solution containing polypeptide for vibration treatment to enable the polypeptide to be coupled to the surface of the anchor line segment through the polydopamine deposition layer to form a polypeptide coating, wherein the amino acid sequence of the polypeptide is K-DFQTWSFLYVEN or DFQTWSFLYVEN, cleaning and drying the anchor line segment with the polypeptide coating formed on the surface, and penetrating a suture on the anchor line segment in a staggered and penetrating mode to obtain the full-suture anchor. The invention has the beneficial technical effects that the polypeptide coating with the capability of specifically activating osteoblast activity is added on the surface of the anchor-like line segment, the osteoblast activity is specifically activated through the polypeptide, the bone repair and tissue bridging of tendon bone interface are promoted, and compared with the traditional full suture anchor, the full suture anchor has stronger capability of promoting tendon bone healing, thereby increasing the mechanical strength of the tendon and bone surface after suturing, reducing the risk of fracture after tendon operation and promoting limb function recovery. Drawings FIG. 1 is a schematic representation of the structure of a full s