CN-122005991-A - Blood component single sampling equipment capable of monitoring in real time

CN122005991ACN 122005991 ACN122005991 ACN 122005991ACN-122005991-A

Abstract

The invention discloses blood component single sampling equipment capable of monitoring in real time in the field of blood treatment equipment. The device comprises a blood sampling module, a centrifugal separation module, a component collection module and an anticoagulant conveying module, and further integrates a multi-dimensional real-time monitoring system and a closed-loop self-adaptive regulation and control module, wherein the multi-dimensional real-time monitoring system comprises an online microfluidic component detection unit, a physiological parameter monitoring unit and a device operation monitoring unit, can realize real-time online detection of blood component purity, cell activity and pollutant residues in the separation process, synchronously monitor circulating physiological parameters of blood donors and device operation states, and the closed-loop self-adaptive regulation and control module can dynamically regulate parameters such as centrifugal rotation speed, fluid pump speed, anticoagulant ratio and the like according to real-time monitoring data so as to realize accurate closed-loop control of the separation process. The invention can obviously improve the quality stability of the blood component single sampling, greatly reduce the operation risk and is suitable for clinical blood component single sampling and preparation scenes.

Inventors

Yang Peirun
CHEN FEI
ZHOU FANGZHU

Assignees

重庆市中医院

Dates

Publication Date: 20260512
Application Date: 20260413

Claims (10)

1. The blood component single sampling device capable of being monitored in real time is characterized by comprising a blood sampling module, a centrifugal separation module, a component collection module, an anticoagulant conveying module, a multidimensional real-time monitoring system and a closed-loop self-adaptive regulation and control module; The output end of the blood sampling module is communicated with the input end of the centrifugal separation module, and a plurality of output ends of the centrifugal separation module are respectively communicated with the corresponding input end of the component collection module and the feedback end of the blood sampling module; The multidimensional real-time monitoring system comprises an online microfluidic component detection unit, a physiological parameter monitoring unit and an equipment operation monitoring unit; the online microfluidic component detection unit is arranged on a component output pipeline of the centrifugal separation module and is used for detecting the purity of the separated blood component, the activity of target cells, the content of free hemoglobin and the residual bacterial pollutants in real time; the physiological parameter monitoring unit is used for collecting heart rate, blood pressure, blood oxygen saturation and circulating blood volume change data of blood donors in real time; the device operation monitoring unit is used for collecting centrifugal rotating speed, pipeline pressure, fluid pump speed and anticoagulant infusion flow data of the device in real time; The closed-loop self-adaptive regulation and control module is respectively and electrically connected with the multidimensional real-time monitoring system, the blood sampling module, the centrifugal separation module, the component collection module and the anticoagulant conveying module and is used for dynamically regulating the operation parameters of the equipment according to the real-time monitoring data so as to realize closed-loop self-adaptive control of the blood component single sampling process.
2. The blood component single sampling device capable of being monitored in real time according to claim 1, wherein the online microfluidic component detection unit comprises a microfluidic chip, a laser excitation module, a fluorescence detection module and a data processing subunit, wherein the microfluidic chip is integrated in a target component output pipeline of a centrifugal separation module, separated blood components continuously flow through a detection channel of the microfluidic chip, and the laser excitation module and the fluorescence detection module are matched to realize counting, activity identification and quantitative detection of impurity components of target cells.
3. The blood component single sampling device capable of being monitored in real time according to claim 2, wherein a sheath flow focusing structure is arranged in a detection channel of the microfluidic chip and used for arranging blood cells flowing through the sheath flow focusing structure into a single row to pass through a detection area so as to improve detection precision, and a contrast channel is further arranged in the microfluidic chip and used for eliminating interference of ambient light and fluid disturbance on a detection result.
4. The blood component single sampling device capable of being monitored in real time according to claim 1, wherein the physiological parameter monitoring unit comprises a wearable acquisition terminal and a data transmission sub-module, the wearable acquisition terminal is worn on the surface of a blood donor, and the physiological data acquired in real time are transmitted to a closed-loop self-adaptive regulation and control module in real time through the data transmission sub-module; The grading early warning and emergency treatment process comprises a first grade early warning, a second grade early warning and a third grade emergency, wherein the first grade early warning is that physiological parameters deviate from a threshold slightly, only a sound and light prompt is touched, equipment operation is not adjusted, the second grade early warning is that physiological parameters deviate from the threshold moderately, a closed loop self-adaptive regulation and control module automatically reduces blood sampling and separation speed, meanwhile, prompts operators to pay attention, the third grade emergency physiological parameters seriously exceed a safety threshold, the closed loop self-adaptive regulation and control module immediately stops blood sampling, a normal saline flushing pipe and blood feedback process is started, and emergency calling is triggered at the same time.
5. The blood component single sampling device capable of being monitored in real time according to claim 1, wherein the centrifugal separation module adopts a continuous flow type centrifugal separation structure and comprises a centrifugal cavity, a separation cup, a rotary driving motor and a multi-channel rotary sealing joint, a gradient separation chamber is arranged in the separation cup, continuous layered separation of plasma, platelets, white blood cells and red blood cells in whole blood can be achieved, the rotary driving motor is electrically connected with a closed-loop self-adaptive regulation and control module, and centrifugal rotation speed can be dynamically regulated according to a detection result of an online microfluidic component detection unit.
6. The blood component single sampling device capable of being monitored in real time according to claim 1, wherein the component collecting module comprises a plurality of independent component collecting units, each component collecting unit corresponds to one separated blood component, an electromagnetic control valve is arranged at the input end of each component collecting unit and is electrically connected with the closed-loop self-adaptive regulation and control module, and when the online microfluidic component detecting unit detects that the purity of the components in the corresponding pipeline does not meet the preset requirement, the closed-loop self-adaptive regulation and control module controls the corresponding electromagnetic control valve to switch, so that the blood components which do not meet the requirement are conveyed to the feedback pipeline to be fed back into the blood donor.
7. The blood component single sampling device capable of being monitored in real time according to claim 1, wherein the anticoagulant conveying module comprises a liquid storage bag, a precise infusion pump and a flow sensor, the precise infusion pump and the flow sensor are electrically connected with a closed-loop self-adaptive regulation and control module, and the closed-loop self-adaptive regulation and control module dynamically adjusts the infusion speed of the precise infusion pump according to blood sampling flow of the blood sampling module and blood circulation volume data of a donor collected by the physiological parameter monitoring unit, so that the ratio of anticoagulant to whole blood is adaptively adjusted.
8. The blood component single sampling device capable of being monitored in real time according to claim 1, wherein a parameter optimization model based on machine learning is built in the closed-loop self-adaptive regulation and control module, the parameter optimization model takes historical single sampling data and real-time monitoring data as input, takes optimal separation parameters as output, and can dynamically match optimal single sampling operation parameters according to individual differences of different blood donors.
9. The real-time monitoring blood component single sampling device according to claim 1, further comprising a data tracing and storage module, wherein the data tracing and storage module is electrically connected with the multi-dimensional real-time monitoring system and is used for storing all monitoring data, device operation parameters and component detection results in the single sampling process in the whole process, generating unique tracing codes and binding corresponding blood components, and realizing the whole process traceability of the blood components from collection to use.
10. The real-time monitoring blood component single sampling device according to claim 1, further comprising a man-machine interaction module electrically connected with the closed-loop adaptive regulation module for displaying real-time monitoring data and device operation state, and supporting an operator to manually set operation parameters, view history data and process early warning information.

Description

Blood component single sampling equipment capable of monitoring in real time Technical Field The invention relates to the field of blood treatment equipment, in particular to blood component single sampling equipment capable of monitoring in real time. Background The blood component collection is a technology for separating and collecting specific components (such as platelets, plasma, stem cells and the like) in whole blood in a centrifugal separation mode, and simultaneously, returning other components into a blood donor body, and is widely applied to scenes such as clinical blood transfusion, blood product preparation, hematopoietic stem cell collection and the like. At present, most of existing blood component single-sampling equipment can only carry out basic monitoring on operation parameters (such as centrifugal rotating speed, pump speed and pipeline pressure) of the equipment, real-time online detection on the quality (such as purity, cell activity and pollutant residue) of separated blood components cannot be realized, quality verification can only be carried out through offline laboratory detection after single-sampling is finished, if component quality does not reach the standard, the equipment can only be scrapped in batches, blood resource waste is caused, separation parameters cannot be adjusted in time, and stability of component quality is difficult to guarantee. In addition, the existing equipment can not synchronously monitor the physiological parameter change of blood donors in the single sampling process, can only rely on manual observation of operators, can not find adverse reactions of the blood donors in time, has certain operation safety risks, and meanwhile, the operation parameters of the equipment are required to be manually set by the operators according to experience, and can not be adaptively adjusted according to individual differences of different blood donors, so that the separation efficiency and the component quality are difficult to be compatible. Accordingly, one skilled in the art would be able to provide a blood component apheresis device that can be monitored in real time to address the problems set forth in the background art above. Disclosure of Invention The invention aims to provide blood component single sampling equipment capable of monitoring in real time so as to solve the problems in the background technology. In order to achieve the above purpose, the present invention provides the following technical solutions: A blood component single sampling device capable of being monitored in real time comprises a blood sampling module, a centrifugal separation module, a component collection module, an anticoagulant conveying module, a multi-dimensional real-time monitoring system and a closed-loop self-adaptive regulation and control module; The output end of the blood sampling module is communicated with the input end of the centrifugal separation module, and a plurality of output ends of the centrifugal separation module are respectively communicated with the corresponding input end of the component collection module and the feedback end of the blood sampling module; The multidimensional real-time monitoring system comprises an online microfluidic component detection unit, a physiological parameter monitoring unit and an equipment operation monitoring unit; the online microfluidic component detection unit is arranged on a component output pipeline of the centrifugal separation module and is used for detecting the purity of the separated blood component, the activity of target cells, the content of free hemoglobin and the residual bacterial pollutants in real time; the physiological parameter monitoring unit is used for collecting heart rate, blood pressure, blood oxygen saturation and circulating blood volume change data of blood donors in real time; the device operation monitoring unit is used for collecting centrifugal rotating speed, pipeline pressure, fluid pump speed and anticoagulant infusion flow data of the device in real time; The closed-loop self-adaptive regulation and control module is respectively and electrically connected with the multidimensional real-time monitoring system, the blood sampling module, the centrifugal separation module, the component collection module and the anticoagulant conveying module and is used for dynamically regulating the operation parameters of the equipment according to the real-time monitoring data so as to realize closed-loop self-adaptive control of the blood component single sampling process. The online microfluidic component detection unit comprises a microfluidic chip, a laser excitation module, a fluorescence detection module and a data processing subunit, wherein the microfluidic chip is integrated in a target component output pipeline of a centrifugal separation module, separated blood components continuously flow through a detection channel of the microfluidic chip, and the laser excitation module and the fluore