CN-122006105-A - Determining TTfield the frequency of treatment based on electrical characteristics of targeted cancer cells

CN122006105ACN 122006105 ACN122006105 ACN 122006105ACN-122006105-A

Abstract

The present application relates to determining TTfield the frequency of treatment based on the electrical characteristics of targeted cancer cells. Cancer treatment using ttfields (tumor treatment fields) can be tailored to each individual subject, by obtaining cancer cells from the subject, determining electrical characteristics (e.g., dielectrophoretic force, cell membrane capacitance, etc.) of the cancer cells, determining a frequency for ttfields based on the determined electrical characteristics, and treating the cancer by applying ttfields to the subject at the determined frequency. In addition, a cancer treatment may be planned for each individual subject, by obtaining cancer cells from the subject, determining an electrical characteristic of the cancer cells, predicting whether TTField will be effective in treating the cancer based on the determined electrical characteristic, and if the prediction indicates that TTField is effective, treating the subject by applying TTField.

Inventors

GHIRARDI MICHELE
E. Zevi
WENGER CORINNE
Z. Bomzon

Assignees

诺沃库勒有限责任公司

Dates

Publication Date: 20260512
Application Date: 20200225
Priority Date: 20190226

Claims (3)

1. A method of selecting a frequency of an alternating electric field for treating cancer in a subject having cancerous tissue, the method comprising: determining a physical parameter of at least one cancer cell extracted from a sample of the cancerous tissue obtained from the subject, and Selecting a frequency of an alternating electric field applied to the subject to treat the cancer based on the determined physical parameter, Characterized in that said physical parameter is how far in the electric field said at least one cancer cell extracted from said sample moves, determined by optical measurement.
2. The method of claim 1, wherein the optical measurement is measuring light intensity of the at least one cancer cell.
3. The method of claim 1, wherein the frequency of the alternating electric field applied to the subject is selected to be a frequency effective in treating reference cancer cells having a known physical parameter, wherein the known physical parameter matches the determined physical parameter.

Description

Determining TTfield the frequency of treatment based on electrical characteristics of targeted cancer cells The application is applied for 25 days of 2 months in 2020, the application number isThe invention is a divisional application of the patent application entitled "determining TTfield frequency of treatment based on electrical characteristics of targeted cancer cells". Cross Reference to Related Applications The present application claims the benefit of U.S. provisional application 62/810,823 filed on 2 months 26 of 2019, which is incorporated herein by reference in its entirety. Background Tumor treatment fields (TTFields) are an effective form of anti-tumor treatment delivered by application of a low intensity (e.g., 1-5V/cm), medium frequency (e.g., 100-300 kHz) alternating electric field. TTField therapy has gained FDA approval for the treatment of glioblastoma multiforme brain tumors and appears to be very promising for many other types of tumors. TTField therapy is delivered using a wearable and portable device (Optune ®). The delivery system includes four adhesive, non-invasive, insulated "transducer arrays", an electric field generator, a rechargeable battery, and a carrying case. The transducer array is applied to the skin near the tumor and connected to a field generator. In a preclinical setting, the Inovitro TM system can be used to apply TTField in vitro through a vertical electrode pair insulated by a high dielectric constant ceramic. Inovitro TM (TTField Lab bench System) contains a TTField generator and a base plate, and each plate contains 8 ceramic disks. Summary of The Invention One aspect of the invention relates to a first method of treating cancer in a subject having cancerous tissue. The first method includes obtaining a sample of the cancerous tissue from the subject, the sample having at least one cancer cell, determining an electrical characteristic of the at least one cancer cell, determining a frequency of an alternating electric field applied to the subject to treat the cancer based on the determined electrical characteristic, and treating the cancer by applying an alternating electric field to the subject at the determined frequency of the alternating electric field. In some cases of the first method, the determining the electrical characteristic includes measuring dielectrophoretic force of the at least one cancer cell at each of a plurality of frequencies below 35 kHz. In some cases of the first method, the determining the frequency includes selecting a frequency effective in treating a reference cancer cell having a known electrical characteristic, wherein the known electrical characteristic matches the determined electrical characteristic. In some cases of the first method, the determining the electrical characteristic comprises determining a cell membrane capacitance. Another aspect of the invention relates to a second method of treating cancer in a subject having cancerous tissue. The second method includes obtaining a sample of the cancerous tissue from the subject, the sample having at least one cancer cell, measuring at least one physical parameter of the at least one cancer cell, wherein an electrical characteristic of the at least one cancer cell can be determined from the at least one physical parameter, determining a frequency of an alternating electric field applied to the subject to treat the cancer based on the measured at least one physical parameter, and treating the cancer by applying an alternating electric field to the subject at the determined frequency of the alternating electric field. In some cases of the second method, the cell membrane capacitance of the at least one cancer cell may be determined from the at least one physical parameter. Another aspect of the invention relates to a third method of treating cancer in a subject having cancerous tissue. The third method includes obtaining a sample of the cancerous tissue from the subject, the sample having at least one cancer cell, determining an electrical characteristic of the at least one cancer cell, predicting whether application of an alternating electric field to the subject will be effective in treating the cancer based on the determined electrical characteristic, and treating the cancer by applying an alternating electric field to the subject if the predicting indicates that application of an alternating electric field to the subject will be effective in treating the cancer. In some cases of the third method, the determining the electrical characteristic comprises measuring dielectrophoretic force of the at least one cancer cell at each of a plurality of frequencies below 35 kHz. In some cases of the third method, the prediction is based on whether a reference cancer cell having an electrical characteristic that matches the determined electrical characteristic is sensitive to treatment with an alternating electric field. In some cases of the third method, the determining the electrical char