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CN-122006287-A - Plant component extraction method based on nano reverse micelle system

CN122006287ACN 122006287 ACN122006287 ACN 122006287ACN-122006287-A

Abstract

The invention provides a plant component extraction method based on a nano reverse micelle system. The method comprises the steps of 1) preparing an extractant composed of grease, a surfactant, water and an alcohol solvent according to a preset proportion, 2) fully mixing plant materials with the extractant, 3) performing ultrasonic extraction or oscillation for a period of time at room temperature, and 4) separating the extract by adopting a centrifugal method or a filtering method and the like to obtain the extract of plant multipolar components. According to the method for extracting plant components by using the nano reverse micelle system, the polar aqueous phase and the alcohol phase are blended into the oil with weak polarity to form a stable system with a reverse micelle structure, so that the traditional thought of similar compatible extraction is broken, and the compatibility and the effective extraction of the oil with weak polarity on the medium and the strong polarity components are realized. The nano reverse micelle system provided by the invention directly extracts and protects high-activity polar components by utilizing the property of good grease sealing property and air isolation, and has excellent application value.

Inventors

  • ZHANG ZHIQIANG
  • LIN RUMEI

Assignees

  • 桂林华章创美生物技术有限公司

Dates

Publication Date
20260512
Application Date
20260213

Claims (8)

  1. 1. The plant component extraction method based on the nano reverse micelle system is characterized by comprising the following process steps of: 1) Preparing an extractant consisting of grease, a surfactant, water and an alcohol solvent according to a preset proportion; 2) Thoroughly mixing plant material with the extractant; 3) Ultrasonic extraction or oscillation is carried out, and the mixture is acted for a period of time at room temperature; 4) Separating the extractive solution by centrifugation or filtration to obtain plant multipolar component extractive solution.
  2. 2. The method of claim 1, wherein the ratio of the aqueous phase, the alcohol phase, the surfactant and the oil phase in the system is controlled to regulate the type and concentration of the polar component; The ratio of the extractant is any one of the following components in percentage by mass: the ratio 1 is 90% of jojoba oil, 8% of polyglycerol ester, 1% of water and 1% of propylene glycol; the ratio of the jojoba oil is 85%, the polyglycerol ester is 10%, the water is 3% and the ethanol is 2%; Ratio 3, jojoba oil 70%, polyglycerol ester 20%, water 5%, ethanol 5%; The proportion is 4, the triglycol caprylate is 50%, the polyglycerol ester is 30%, the water is 10% and the ethanol is 10%.
  3. 3. The method of claim 1, wherein the plant material is selected from the group consisting of pollen Granati, pericarpium Granati, radix astragali, whole plant of astronomical grass, olive leaf, turmeric, grape seed, rhizome of Polygonum cuspidatum, perilla leaf, green tea leaf, and ginseng root, or a combination thereof.
  4. 4. The method of claim 1, further comprising the step of adjusting during the extraction by ultrasonic extraction for 15 to 30 minutes, or shaking for 30 minutes.
  5. 5. Use of the plant component extraction process based on nano-reverse micelle systems according to any one of claims 1 to 4 for the preparation of plant extracts for use in skin care or cosmetics.
  6. 6. The method of claim 5, wherein the plant extract is extracted from the nano reverse micelle system and comprises one or more active ingredients selected from polyphenols, flavonoids, saponins, tannins, anthocyanidins and the like.
  7. 7. The extraction method of the pomegranate polyphenol based on the nano reverse micelle system is characterized by comprising the following process steps of: Adding 3% of pure water and 2% of ethanol into a container, and uniformly mixing; step two, adding 10% of polyglycerol ester into a container, and uniformly mixing; step three, adding jojoba oil 85%, uniformly stirring, and homogenizing for 30 seconds for later use; adding pericarpium Granati powder, stirring slowly for 1 min, and soaking for one hour; Fifthly, carrying out ultrasonic extraction on the materials, wherein the working frequency is 20kzh, the time is 30 minutes, and the temperature is controlled to be less than 60 ℃, and the ultrasonic extraction can be carried out in a cold water bath; Step six, filtering the obtained extract liquid (the filtering pore diameter is smaller than 10 mu m); And seventhly, storing the filtrate in a clean container to obtain the pomegranate polyphenol extract oil.
  8. 8. The extraction method of the astronomical grass spilanthol based on the nano reverse micelle system is characterized by comprising the following process steps: Adding 10% of pure water and 10% of ethanol into a container, and uniformly mixing; Step two, adding 30% of polyglycerol ester into a container, and uniformly mixing; step three, adding 50% of triglycol octyldecanoate, uniformly stirring, and homogenizing for 30 seconds for later use; adding the whole astronomical grass powder, stirring slowly for 1 minute, and soaking for one hour; Fifthly, carrying out ultrasonic extraction on the materials, wherein the working frequency is 20kzh, the time is 30 minutes, and the temperature is controlled to be less than 60 ℃, and the ultrasonic extraction can be carried out in a cold water bath; Step six, filtering the obtained extract liquid (the filtering pore diameter is smaller than 10 mu m); and seventhly, storing the filtrate in a clean container to obtain the astronomical grass extracted oil.

Description

Plant component extraction method based on nano reverse micelle system Technical Field The invention relates to the technical field of plant extraction, in particular to a plant component efficient extraction method based on a nano reverse micelle system and application thereof. Background In traditional plant extraction, the nonpolar or medium polar component is extracted by using a multi-purpose oil solvent, while the polar molecular component is generally extracted by using water or polar alcohol solvent. The extraction mode of single polarity matching has poor compatibility, and cannot be used for extracting plant polyphenol and other application scenes from grease. In the current plant extraction, for various molecules containing different molecular groups, extraction and separation preservation are carried out on the principle of similar compatibility in most cases, for example, water and alcohol with strong polarity are used for extracting glycoside substances containing hydroxyl groups, and grease with weak polarity is used for extracting terpene substances and ester substances containing medium and long carbon chains. The prior extraction technology has to be separately and independently treated according to the polarity intensity, the extracted plants cannot be fully utilized, or the recycling cost is high, and the extraction technology cannot meet the requirement when the multi-component product is required to be extracted. Therefore, only a part of components can be selected for extraction during extraction, and multiple components with polarity and non-polarity are required to be extracted, and then the components are required to be extracted separately and independently for recombination. In the prior art, the molecules with strong polarity and the nonpolar molecules are required to be extracted respectively and then are subjected to subsequent synthesis treatment, so that the cost and the complexity are increased. Chinese patent CN2015111931. X discloses a method for synchronously extracting protein and grease from Chinese yew seeds, which mainly comprises the steps of adding crushed Chinese yew seeds into a reverse micelle system for extraction, performing pre-extraction and post-extraction, performing centrifugal separation to obtain a water phase containing protein and an organic phase containing oil, performing dialysis treatment on the water phase containing protein to obtain Chinese yew seed protein, and extracting the organic phase containing oil to obtain Chinese yew seed oil. At present, the method for extracting various polar components of plants by using a nano reverse micelle system is rarely reported, so that the method has very important significance for researching the efficient extraction function of the plant. Disclosure of Invention Based on the above circumstances, the invention aims to provide a plant component extraction method based on a nano reverse micelle system, which can improve the plant utilization rate, reduce the extraction time and protect the extracted high-activity polar component by utilizing the property of grease isolation from air. In order to achieve the above object, the preparation method adopted by the invention is as follows: A high-efficiency extraction method of plant components based on a nano reverse micelle system comprises the following steps: 1) Preparing an extractant consisting of grease, a surfactant, water and an alcohol solvent according to a preset proportion; 2) Thoroughly mixing plant material with the extractant; 3) Ultrasonic extraction or oscillation is carried out, and the mixture is acted for a period of time at room temperature; 4) Separating the extractive solution by centrifugation or filtration to obtain plant polar component extractive solution. In the efficient extraction method of the plant components, the ratio of the extracting agents is as follows: the ratio 1 is that jojoba oil 90%, polyglycerol ester 8%, water 1%, propylene glycol 1% are used for extracting weak polar components; the ratio 2 is that jojoba oil 85%, polyglycerol ester 10%, water 3%, ethanol 2% are used for extracting medium-polarity components; the ratio 3 is that jojoba oil 70%, polyglycerol ester 20%, water 5%, ethanol 5% are used for extracting medium-polarity components; proportion 4, caprylic capric acid triglycol ester 50%, polyglycerol ester 30%, water 10%, ethanol 10% are used for a strong polar system. In the method for efficiently extracting plant components, the plant material is selected from any one or a combination of pollen Granati, pericarpium Granati, radix astragali, herba Euphorbiae Humifusae whole part, folium Canarii albi, curcuma rhizome, grape seed, rhizoma Polygoni Cuspidati rhizome, folium Perillae, green tea and radix Ginseng. In the efficient extraction method of the plant components, ultrasonic extraction is adopted for 15 to 30 minutes or vibration is carried out for 30 minutes in the extraction process, so that the extracti