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CN-122006477-A - Ultrafiltration dialyzer, ultrafiltration device and filtration method

CN122006477ACN 122006477 ACN122006477 ACN 122006477ACN-122006477-A

Abstract

The invention discloses an ultrafiltration dialyzer, an ultrafiltration device and a filtration method, and belongs to the technical field of biological separation and purification. The ultrafiltration dialyzer comprises a filter cup, a cover plate and a filter tube with a detachable filter membrane, and can realize positive pressure/negative pressure dual-mode driving filtration. The ultrafiltration device is provided with an upper group of rotary tray components and a lower group of rotary tray components which can rotate independently, the trays can accommodate a plurality of groups of ultrafiltration dialyzers, and the air pressure regulating mechanism is communicated through an integrated air circuit of the rotary upright post, so that multi-station batch synchronous filtration is realized. The invention is matched with two sets of positive and negative pressure filtering processes, meets the treatment requirements of different protein solutions, has convenient equipment disassembly and assembly and strong adaptability, and can obviously improve the efficiency and the operation flexibility of protein filtering treatment.

Inventors

  • JIN ZIXIN
  • JIAO XINYAO
  • WANG LEI
  • ZHENG BOYANG
  • WANG JING
  • SHI NINGNING
  • WU MENGQI
  • ZHANG HAOCHEN
  • LIU HUAYING
  • ZHANG LUSHI

Assignees

  • 河北医科大学

Dates

Publication Date
20260512
Application Date
20260413

Claims (10)

  1. 1. An ultrafiltration dialyzer, which comprises a main body, characterized in that it comprises: A filter cup (1), one side of which is provided with a cup body joint (8); The cover plate (7) is detachably arranged at the top of the filter cup (1); The filter tube (2) is arranged on the cover plate (7), the bottom of the filter tube is positioned in the filter cup (1) and is detachably provided with a filter membrane (9), the top of the filter tube protrudes out of the cover plate (7) and is detachably provided with a nut joint assembly (6), and the filter tube (2) is used for containing a solution to be filtered; The cup body joint (8) or the nut joint assembly (6) is connected with the air pressure adjusting mechanism through a pipeline, so that the air pressure above the solution to be filtered is greater than the air pressure in the filter cup (1), and the solution to be filtered is driven to pass through the filter membrane (9).
  2. 2. The ultrafiltration dialyzer according to claim 1, wherein a tabletting nut (4) is screwed at the lower part of the filter tube (2), a filter membrane supporting sheet (3) is arranged between the tabletting nut (4) and the filter tube (2), the filter membrane supporting sheet (3) is used for supporting and fixing the filter membrane (9), a filtrate passing part is arranged at the middle part of the filter membrane supporting sheet (3), a through hole is arranged at the middle part of the tabletting nut (4) corresponding to the middle part of the filter membrane supporting sheet (3), and the tabletting nut (4) is screwed to tightly fix the filter membrane supporting sheet (3) and the filter membrane (9) at the lower end of the filter tube (2).
  3. 3. An ultrafiltration device comprising the ultrafiltration dialyzer of claim 1, comprising: A swivel base (300) provided with a base joint (301), the base joint (301) being connected with the air pressure adjusting mechanism; The ultrafiltration dialysis device comprises two groups of rotary tray assemblies which are distributed up and down, wherein each rotary tray assembly comprises a rotary upright post (400) and a tray (200) sleeved on the corresponding rotary upright post (400), the tray (200) is used for accommodating a plurality of ultrafiltration dialyzers, upright post cavities are arranged in the corresponding rotary upright posts (400) and are connected with the corresponding ultrafiltration dialyzers through air pipe assemblies (500), the lower rotary upright posts (400) are rotatably connected with a rotary base (300), the upper rotary upright posts (400) and the lower rotary upright posts (400) are rotatably connected, the upright post cavities of the lower rotary tray assemblies are communicated with base joints (301), and the upright post cavities of the two groups of rotary tray assemblies are communicated.
  4. 4. An ultrafiltration device according to claim 3, wherein the rotary column (400) is cylindrical, the lower end thereof is provided with an annular column bottom flange (402), the top thereof is provided with a plurality of threaded holes in an annular shape, a plurality of column joints (401) are uniformly arranged on the circumference of the side wall of the middle part of the rotary column (400), the column joints (401) are communicated with the column cavity, and the column joints (401) are connected with a cup joint (8) or a nut joint assembly (6) of the ultrafiltration dialyzer through the air pipe assembly (500).
  5. 5. The ultrafiltration device according to claim 4, wherein the tray (200) is disc-shaped as a whole, a plurality of accommodating grooves (201) are uniformly formed in the upper circumference of the tray, and the filter cup (1) of the ultrafiltration dialyzer is embedded in the accommodating grooves (201); a tray groove (202) is formed in the middle of the lower end face of the tray (200), a through column through hole is formed in the tray groove (202) corresponding to the rotary column (400), the rotary column (400) penetrates through the column through hole, the column bottom flange (402) is embedded into the tray groove (202), and then a bolt assembly is arranged in a penetrating mode to connect the tray (200) with the column bottom flange (402).
  6. 6. The ultrafiltration device according to claim 5, further comprising two sets of swivel bearings (900), the contour of the outer diameter of the swivel bearings (900) corresponding to the contour of the edge of the tray groove (202); The rotary base (300) is provided with a rotary support table (303), the inner ring of a rotary bearing (900) is fixedly connected with the rotary support table (303) through a penetrating bolt assembly, and the outer ring of the rotary bearing (900) is connected with a tray (200) of the rotary tray assembly below through a penetrating bolt assembly; the top of the rotary upright column (400) of the rotary tray assembly is provided with an end cover (800), the end cover (800) is fixedly connected with the inner ring of the other rotary bearing (900) through a penetrating bolt assembly, and the outer ring of the rotary bearing (900) is connected with the tray (200) of the rotary tray assembly above through a penetrating bolt assembly.
  7. 7. An ultrafiltration device according to claim 6, wherein the bottom of the column cavity is provided with a core plate (403), and wherein the core plate (403) is provided with a column bottom joint (404) in communication with the column cavity; The base joint (301) is connected with a column bottom joint (404) of the rotary tray assembly below; an end cover joint (801) communicated with the column cavity of the rotary tray assembly below is arranged on the end cover (800), and the end cover joint (801) is connected with the column bottom joint (404) of the rotary tray assembly above.
  8. 8. A filtration method based on the ultrafiltration device according to any one of claims 3 to 7, characterized by the steps of: S1, selecting a negative pressure driving filtration mode when the aperture of the filter membrane (9) is more than or equal to 0.1 mu m; S2, connecting a cover plate (7) with the filter pipe (2), putting a transparent container for receiving the filtrate in the filter cup (1), and then installing the cover plate (7) on the filter cup (1), connecting and fixing one end of the air pipe assembly (500) with a column joint (401) on the rotary column (400), and closing air pipe valves on the air pipe assemblies (500); S3, starting negative pressure vacuum pump equipment connected with the base joint (301), and adjusting the air pressure in the cavities of the two upright posts to be between gauge pressure of-0.1005 and-0.08 MPa; S4, opening the nut joint assembly (6), adding the solution to be filtered into the filter pipe (2) by using a pipettor, enabling the liquid level of the solution to be filtered to be lower than a preset height, and then closing the nut joint assembly (6); s5, connecting the other end of the air pipe assembly (500) with a cup body joint (8), connecting the air pipe of the gauge (600) with a nut joint assembly (6), and opening air pipe valves on each air pipe assembly (500) for filtering; s6, periodically rotating the tray (200) to observe and record the filtering state in the filtering cup (1); s7, after filtration is completed, an air pipe valve on the air pipe assembly (500) is closed, connection between the air pipe assembly (500) and the cup body connector (8) is disconnected, connection between an air pipe of the meter (600) and the nut connector assembly (6) is disconnected, the cover plate (7) and the filter pipe (2) are taken down, the transparent container is taken out, and concentrated solution in the filter pipe is sucked out and stored.
  9. 9. The filtering method according to claim 8, wherein the meter (600) comprises a metering shaft (601) and a metering tube (602) wound on the metering shaft (601), a metering scale (603) is arranged on the metering tube (602), an indicating liquid section is arranged in the metering tube (602), and when the liquid level in the filtering tube (2) is lowered, the indicating liquid section moves to indicate the filtering process; One end of the metering tube (602) is empty, and the other end of the metering tube is provided with an air inlet valve (604) for being connected with the nut joint assembly (6).
  10. 10. A filtration method based on the ultrafiltration device according to any one of claims 3 to 7, characterized by the steps of: s1, selecting a positive pressure driven filtration mode according to the sample filtration requirement of a filter membrane (9) with the aperture smaller than 0.1 mu m, selecting a proper filter membrane supporting sheet (3), and placing an ultrafiltration device in a sterile environment; s2, connecting a cover plate (7) with the filter pipe (2), putting a transparent container for receiving solution in the filter cup (1), and then installing the cover plate (7) on the filter cup (1), connecting and fixing one end of the air pipe assembly (500) with a column joint (401) on the rotary column (400), and closing air pipe valves on the air pipe assemblies (500); s3, starting air compressor equipment connected with the base joint (301), and adjusting the air pressure in the cavities of the two upright posts to be between 0.2 and 0.6 MPa; S4, opening the nut joint assembly (6), adding the solution to be filtered into the filter pipe (2) by using a pipettor, enabling the liquid level of the solution to be filtered to be lower than a preset height, and then closing the nut joint assembly (6); s5, connecting the other end of the air pipe assembly (500) with the nut joint assembly (6), leaving the cup joint (8) empty, and opening air pipe valves on each air pipe assembly (500) for filtering; s6, periodically rotating the tray (200) to observe and record the filtering state in the filtering cup (1); S7, after the filtration is completed, an air pipe valve on the air pipe assembly (500) is closed, the air pipe assembly (500) is disconnected from the nut joint assembly (6), the cover plate (7) and the filter pipe (2) are taken down, and the transparent container is taken out.

Description

Ultrafiltration dialyzer, ultrafiltration device and filtration method Technical Field The invention relates to a protein ultrafiltration concentration device and a method, in particular to an ultrafiltration dialyzer, an ultrafiltration device and a filtration method. Background Ultrafiltration is a core technology of protein purification, concentration, desalination and buffer replacement treatment, and is widely applied to the fields of biopharmaceutical and life science research. The current laboratory protein ultrafiltration device is a commercial ultrafiltration tube with a single-channel structure, the ultrafiltration scheme has the following defects that ① the ultrafiltration tube is designed into a mode of nested filter membranes in a standard centrifuge tube, the ultrafiltration tube is limited by the rotor volume of the centrifuge, the maximum single-tube sample capacity is 15mL, the sample size in the interval of 15-50mL is lower than the minimum treatment capacity of a tangential flow ultrafiltration membrane bag or a hollow fiber column and is multiplied by the single-tube capacity of the commercial ultrafiltration tube, so that the sample in the interval of concentrating the sample is required to be frequently started and stopped, the cover is opened for supplementing liquid and discarding filtrate, ② the ultrafiltration tube cannot visually judge the filtrate and the concentration multiple in the centrifuge, the observation liquid of each tube is required to be taken out after the centrifuge is frequently started and stopped, the operation is very complicated, the filter membrane supporting part of the commercial ultrafiltration tube ③ is a plastic grid, the grid gap is large, the strength is low, the upper limit of the centrifugal driving force is very low, the ultrafiltration speed is slow, and ④ commercial ultrafiltration tubes and the membranes are disposable products, and the cost is not environment-friendly, and the batch treatment efficiency and the cost controllability cannot be considered. Disclosure of Invention The invention aims to solve the technical problem of providing an ultrafiltration dialyzer, an ultrafiltration device and a filtration method which have the advantages that only a filter membrane is disposable and replaceable, a plurality of groups of ultrafiltration dialyzers can be flexibly operated in parallel and the filtrate amount can be conveniently observed. In order to solve the problems, the invention adopts the following technical scheme: the key technology of the ultrafiltration dialyzer is that the ultrafiltration dialyzer comprises: A cup body joint is arranged on one side of the filter cup; the cover plate is detachably arranged at the top of the filter cup; the filter pipe is arranged on the cover plate, the bottom of the filter pipe is positioned in the filter cup and is detachably provided with a filter membrane, the top of the filter pipe protrudes out of the cover plate and is detachably provided with a nut joint assembly, and the filter pipe is used for containing solution to be filtered; The cup body joint or the nut joint component is connected with the air pressure adjusting mechanism through a pipeline, so that the air pressure above the solution to be filtered is greater than the air pressure in the filter cup, and the solution to be filtered is driven to pass through the filter membrane. As one embodiment of the invention, a pressing nut is connected to the lower part of the filter tube in a threaded manner, a filter membrane supporting plate is arranged between the pressing nut and the filter tube, the filter membrane supporting plate is used for supporting and fixing the filter membrane, a filtrate passing part is arranged in the middle of the filter membrane supporting plate, a through hole is arranged in the middle of the pressing nut corresponding to the middle of the filter membrane supporting plate, and the pressing nut is screwed to tightly press and fix the filter membrane supporting plate and the filter membrane at the lower end of the filter tube. The ultrafiltration device based on the ultrafiltration dialyzer is characterized by comprising the following components: The rotary base is provided with a base joint which is connected with the air pressure adjusting mechanism; The rotary tray assembly comprises rotary upright posts and trays sleeved on the rotary upright posts, wherein the trays are used for accommodating a plurality of ultrafiltration dialyzers, upright post cavities are arranged in the rotary upright posts and are connected with the ultrafiltration dialyzers through air pipe assemblies, the lower rotary upright posts are rotatably connected with the rotary base, the upper rotary upright posts and the lower rotary upright posts are rotatably connected, the upright post cavities of the lower rotary tray assembly are communicated with the base joint, and the upright post cavities of the two rotary tray assemblies are communi