CN-122006502-A - Photo-thermal sterilization anticoagulation hemodialysis membrane and preparation method thereof

Abstract

The invention belongs to the technical field of membrane separation materials and preparation thereof, and provides an anticoagulant hemodialysis membrane capable of photo-thermal sterilization and a preparation method thereof. The hemodialysis membrane consists of a hemodialysis membrane substrate, a nano coating combined on the hemodialysis membrane substrate and anticoagulant molecules anchored on the nano coating, wherein the nano coating is positioned on the surface and in a pore channel structure of the hemodialysis membrane substrate, the nano coating consists of nano particles, the nano particles are copper ion crosslinked polydopamine nano particles formed by polymerizing dopamine in a solution under the catalytic oxidation of copper ions and hydrogen peroxide and adhering to the hemodialysis membrane substrate, and the anticoagulant molecules are anchored on the nano coating. The invention endows the hemodialysis membrane with photo-thermal sterilization performance and anticoagulation performance, can avoid adverse effect on anticoagulation performance caused by photo-thermal sterilization performance, and improves the hydrophilicity of the hemodialysis membrane.

Inventors

• XIE RUI
• WANG YUJUE
• CHU LIANGYIN
• JU XIAOJIE
• LIU ZHUANG
• WANG WEI
• PAN DAWEI

Assignees

• 四川大学

Dates

Publication Date

20260512

Application Date

20260323

Claims (10)

1. 1. The photo-thermal sterilization anti-coagulation hemodialysis membrane is characterized by comprising a hemodialysis membrane substrate, a nano coating combined on the hemodialysis membrane substrate and anti-coagulation molecules anchored on the nano coating, wherein the nano coating is positioned on the surface and pore channel structure of the hemodialysis membrane substrate, the nano coating consists of nano particles, the nano particles are copper ion crosslinked poly-dopamine nano particles formed by polymerizing and adhering dopamine on the hemodialysis membrane substrate in a solution under the catalytic oxidation of copper ions and hydrogen peroxide, the anti-coagulation molecules are bivalirudin, and the anti-coagulation molecules are anchored on the nano coating through Miceal addition, schiff base reaction and coordination combination with the copper ions, and the hemodialysis membrane has photo-thermal sterilization and anti-coagulation performances.
2. 2. The photo-thermal sterilization anticoagulant hemodialysis membrane according to claim 1, wherein the content of polydopamine in the hemodialysis membrane is 140-170 μg/cm 2 .
3. 3. The photo-thermal sterilization anticoagulant hemodialysis membrane according to claim 2, wherein the content of copper ions in the hemodialysis membrane is 10-28 μg/cm 2 .
4. 4. A photo-thermal sterilizable anticoagulant hemodialysis membrane according to any one of claims 1 to 3, wherein a wet water contact angle of the hemodialysis membrane surface is 11 ° to 15 °.
5. 5. A photo-thermally sterilizable anticoagulant hemodialysis membrane according to any one of claims 1 to 3, characterized in that the anticoagulant properties of anticoagulant molecules on the hemodialysis membrane are not affected below 65 ℃.
6. 6. A photo-sterilizable anticoagulant hemodialysis membrane according to any one of claims 1 to 3, wherein the hemodialysis membrane substrate comprises any one of a cellulose membrane, a polyamide membrane, a polysulfone membrane, a polyethersulfone membrane, a polyacrylonitrile membrane, a polymethyl methacrylate membrane, an ethylene-vinyl alcohol copolymer membrane, and a polyester polymer alloy membrane.
7. 7. The method for producing a photo-heat sterilizable anticoagulant hemodialysis membrane according to any one of claims 1 to 6, comprising the steps of: (1) Fully soaking the hemodialysis membrane substrate with deionized water to remove impurities; (2) Dissolving dopamine, cuSO 4 and H 2 O 2 in water, and adjusting the pH value to 8-9 to obtain a deposition solution, wherein the concentration of the dopamine in the deposition solution is 1-20 mg/mL, the concentration of the CuSO 4 in the deposition solution is 5-15 mmol/L, H 2 O 2 , and the concentration of the CuSO 4 in the deposition solution is 9.8-32.6 mmol/L; Immersing the hemodialysis membrane substrate with the impurities removed in the deposition solution, reacting for 3-24 hours at 25-75 ℃ in an air atmosphere, washing, and drying to obtain a membrane substrate with a nano coating deposited; (3) Dissolving anticoagulation molecule in physiological saline to obtain reaction liquid, immersing the film substrate deposited with nano coating in the reaction liquid for full reaction, washing and drying to obtain the anticoagulation hemodialysis film capable of photo-thermal sterilization.
8. 8. The method for preparing an anticoagulant hemodialysis membrane capable of photo-thermal sterilization according to claim 7, wherein in the step (3), the membrane substrate deposited with the nano coating is immersed in a reaction solution to react for 6-24 hours at 25-37 ℃.
9. 9. The method for producing a photo-thermal sterilizable anticoagulant hemodialysis membrane according to claim 7 or 8, wherein the concentration of anticoagulant molecules in the reaction solution of the step (3) is 100-300 μg/mL.
10. 10. The method for producing a photo-heat sterilizable anticoagulant hemodialysis membrane according to claim 7 or 8, wherein in the step (3), stirring or shaking is applied during the reaction of immersing the membrane substrate on which the nano-coating is deposited in the reaction liquid.

Description

Photo-thermal sterilization anticoagulation hemodialysis membrane and preparation method thereof Technical Field The invention belongs to the technical field of membrane separation materials and preparation thereof, and relates to an anticoagulant hemodialysis membrane capable of photo-thermal sterilization and a preparation method thereof. Background Acute/chronic kidney disease, caused by abnormal kidney structure or function, results in loss of functional nephrons (irreversible) and often progresses to end stage renal disease, i.e., renal failure. Currently, kidney replacement therapies that can extend the life of a patient are mainly kidney transplantation, peritoneal dialysis and hemodialysis. Among them, hemodialysis is the most commonly used treatment means, which has advantages of higher efficiency and sufficiency compared to peritoneal dialysis, and is particularly suitable for rapid treatment of pulmonary edema, hyperkalemia and drug poisoning. Hemodialysis membranes are the core component of hemodialysis treatment, however, membrane materials that contact blood are susceptible to triggering the coagulation cascade to cause coagulation. Meanwhile, the incidence rate and the death rate of acquired blood flow infection of patients and related personnel in the hospital are high due to the early preparation operation of hemodialysis and the blood medical waste generated later. In addition, studies have found that certain bacteria/viruses exhibit affinity for fibrin and platelet-attached surfaces, thereby infecting/damaging endothelial cells and inducing their procoagulant properties, producing tissue factors, altering protein levels, etc., ultimately leading to bleeding or clotting. Coagulation and infection not only lead to increased treatment difficulty, but also seriously threaten the life safety of patients. Therefore, research and preparation of hemodialysis membranes with antibacterial and anticoagulant properties are of great significance. The sterilization methods of medical instruments are mainly divided into physical type (such as gamma rays, E electrons, microwaves, ultraviolet rays, dry heat, steam and the like), chemical type (such as gas ethylene oxide, steam H 2O2 and the like) and composite type (such as H 2O2 plasma, supercritical CO 2 and the like). Among them, high pressure steam (heat penetration) is used as a conventional steam sterilization method, which can satisfy sterilization of complex structures and blood-contaminated materials in the treatment of medical materials, and has no harmful substance residues and leakage. Phototherapy (Photothermal therapy, PTT) has received much attention for advantages of mild conditions, low energy consumption, small influence on materials, etc., compared to conventional steam sterilization (121-141 ℃, 206-348 kPa). Phototherapy relies on photothermal agents to convert light energy of near infrared light (NEAR INFRARED, NIR) into heat, causing a rapid local temperature rise, resulting in a phase change of the cell membrane/wall of bacteria to achieve sterilization. Currently, researchers have successfully utilized PTT and its synergism (e.g., PTT/photodynamic therapy, PTT/chemo-dynamic therapy, etc.) to obtain antimicrobial materials with sterilization rates as high as 95% or more. To alleviate the coagulation problem in dialysis treatment, it is mainly selected clinically to inject a certain amount of anticoagulant (commonly heparin) into the dialyzed blood to cut off one or more links in the coagulation mechanism to achieve anticoagulation. Researchers have improved membrane hemocompatibility by modifying the materials, the main strategies of which include building anti-fouling surfaces, introducing anticoagulants or anticoagulation groups. By constructing an anti-fouling surface (such as a hydrophilic surface), the adhesion aggregation of proteins, blood cells and the like can be resisted, and the activation of a coagulation path due to the conformational change of the proteins, the activation of blood platelets and the like can be prevented. However, the antifouling effect of the anti-adhesion surface in complex pathological environments tends to be gradually reduced over time, so that even a very small bacterial adhesion of the anti-adhesion surface is sufficient to initiate the diffusion of biological contaminants to form a biofilm, which becomes one of the causes of thrombosis. An anticoagulant substance capable of specifically anticoagulating, anticoagulant groups (such as-SO 3 H, -COOH, -OH and the like) or anticoagulant group-containing materials (such as polysaccharide and the like) are introduced into the membrane material by a chemical/physical method, SO that the anticoagulation performance of the membrane material is endowed. However, sterilization processes prior to dialysis may destroy the anticoagulant activity of the material. It is found that the high temperature conditions during autoclaving lead to reduced hydrophilicity