CN-122006539-A - Emulsifying device and preparation method of colostrum thereof

CN122006539ACN 122006539 ACN122006539 ACN 122006539ACN-122006539-A

Abstract

The application discloses an emulsifying device and a preparation method of primary emulsion of the emulsifying device, which belong to the technical field of microfluidic emulsification, and comprise an upper cover and a valve seat, wherein the upper cover is fixedly connected with the valve seat to form an emulsifying device main body, a micro-pore plate is arranged between the upper cover and the valve seat, a micro-pore channel is arranged on the micro-pore plate and is used for realizing focusing emulsification of a disperse phase and a mobile phase, a sealing element is arranged between the upper cover and the valve seat and is used for realizing axial and radial sealing, a mobile phase inlet is arranged on the side part of the valve seat or the upper cover and is used for introducing the mobile phase, the disperse phase inlet is arranged at the top of the upper cover or the valve seat and is used for introducing the disperse phase, a mixed phase outlet is used for discharging emulsified mixed liquid, and the emulsifying device main body is configured to realize continuous regulation and control of particle size of emulsified liquid drops under the condition that the micro-pore plate is not replaced. The application has the effect of realizing continuous regulation and control of the particle size of the emulsion liquid drops by regulating the flow velocity of the mobile phase and the disperse phase on the premise of not replacing the micro-pore plate.

Inventors

LIU WEIHUA
YIN YUNFEI
HUANG YU
YE ZHICHEN
LU YI
HUANG SIYUAN
LU KAI
CHENG SONG
HUANG SHUANGQUAN
CHEN WEIXU

Assignees

江苏洁维生物设备股份有限公司

Dates

Publication Date: 20260512
Application Date: 20260407

Claims (10)

1. An emulsifying device for the water-soluble organic solvent, characterized by comprising the following steps: The upper cover (1) and the valve seat (2) are fixedly connected in a detachable mode to form an emulsifier main body; the micropore plate (3) is arranged between the upper cover (1) and the valve seat (2), and at least one micropore channel is arranged on the micropore plate (3) and used for realizing focusing emulsification of a disperse phase and a mobile phase; the sealing piece (4) is arranged between the upper cover (1) and the valve seat (2) and is used for realizing axial and radial sealing; At least one mobile phase inlet (5) provided at the side of the valve seat (2) or the upper cover (1) for introducing a mobile phase; a disperse phase inlet (6) arranged at the top of the upper cover (1) or the valve seat (2) and used for introducing a disperse phase; A mixed phase outlet (7) arranged at the bottom of the valve seat (2) and used for discharging emulsified mixed liquid; Wherein the mobile phase inlet (5) and the disperse phase inlet (6) are in non-coplanar intersection at the micropore plate (3) to form a three-dimensional flow focusing structure, so that the mobile phase generates shearing force relative to the disperse phase to break the mobile phase to form liquid drops; The emulsifier body is configured to achieve continuous regulation of the particle size of emulsified droplets without replacing the microplate (3) by adjusting the flow rate of the mobile phase and the dispersed phase.
2. An emulsifier as claimed in claim 1, wherein the emulsifier body is connected to an external pumping system for independently adjusting the flow rates of the mobile phase and the disperse phase, and wherein continuous adjustment of droplet size is achieved by changing the flow rate ratio of the mobile phase to the disperse phase, wherein increasing the mobile phase flow rate or decreasing the disperse phase flow rate decreases the droplet size and vice versa.
3. The emulsifying device of claim 1, wherein a jacket structure (8) is arranged outside the emulsifying device main body, the jacket structure (8) can be connected with a temperature control unit, and the temperature control unit can accurately control the temperature in the emulsifying process.
4. An emulsifier according to claim 1, wherein the sealing element (4) is designed as a circular ring, the sealing element (4) is arranged in an annular groove between the upper cover (1) and the valve seat (2), and the sealing element (4) is simultaneously contacted with the inner wall of the upper cover (1) and the outer wall of the valve seat (2).
5. An emulsifier according to claim 1, wherein the number of the mobile phase inlets (5) is two, the two mobile phase inlets (5) are symmetrically arranged on two sides of the main body of the emulsifier, the axes of the mobile phase inlets (5) and the axes of the disperse phase inlets (6) are intersected at the micropore plate (3) to form symmetrical shearing force fields, so that the disperse phase is uniformly sheared and broken at the micropore channel inlets.
6. The emulsifier of claim 1, wherein the upper cover (1) is provided with an optical detection interface (9) for observing the droplet forming process at the micro-pore plate (3) in real time and accessing an online detection system for particle size monitoring and feedback regulation, and the online detection system automatically adjusts the flow rate of the mobile phase and the disperse phase according to the monitored particle size data to realize closed-loop control of particle size.
7. The emulsifier of claim 1 wherein the emulsifier body is of modular construction, wherein a plurality of emulsifier bodies are configured for use in series or in parallel to form a multistage emulsification system, wherein emulsion from a preceding stage of emulsifier is used as feed to a subsequent stage of emulsifier for a stepwise reduction in particle size or multiple emulsification process, and wherein the emulsion is used in parallel to increase throughput of emulsification process.
8. An emulsifier according to claim 1, wherein a control system is arranged on the emulsifier body, a pressure sensor is arranged at the position of the mobile phase inlet (5) and the disperse phase inlet (6), the pressure sensor is connected with the control system, and the pressure sensor monitors inlet pressure in real time.
9. An emulsifier according to claim 1, characterized in that the mixed phase outlet (7) is provided with a diverging structure or buffer chamber.
10. A method of preparing colostrum, an emulsifier according to any one of claims 1 to 9, comprising the steps of: S1, pumping a mobile phase into an emulsifying body through a mobile phase inlet (5), and pumping a disperse phase into the emulsifying body through a disperse phase inlet (6); S2, adjusting the flow velocity of the mobile phase and the disperse phase to enable the mobile phase and the disperse phase to form a three-dimensional flow focusing structure at the position of the micropore plate (3), and enabling the mobile phase to generate shearing force relative to the disperse phase to break to form liquid drops; S3, adjusting the temperature in the main body of the emulsifier to a preset temperature range through a temperature control unit, so that the elasticity of the materials of the disperse phase and the mobile phase is in an optimal state; s4, discharging the emulsified mixed liquid from a mixed phase outlet (7) to obtain the colostrum with uniform particle size.

Description

Emulsifying device and preparation method of colostrum thereof Technical Field The invention relates to the technical field of microfluidic emulsification, in particular to an emulsifier and a preparation method of colostrum thereof. Background The flow focusing technology in the micro-jet method is an important development direction of micro-fluidic emulsification because of the advantages of compact structure, controllable particle size and the like, in the flow focusing type emulsifier, a disperse phase and a mobile phase are intersected at a micro-pore passage, and the mobile phase breaks the disperse phase to form liquid drops through shearing force. However, the liquid drop particle size of the traditional emulsifier is mainly determined by the size of the micropores of the microporous plate, the micropores are equivalent to a filter screen, when the particle size needs to be changed, the microporous plates with different specifications must be stopped and replaced, the operation is complicated, the efficiency is low, the equipment maintenance cost and the production interruption time are increased, the flexible production requirements of multiple varieties and small batches cannot be met, and more importantly, the design concept of the one-hole fixed particle size is more critical, so that the emulsifier lacks the online regulation and control capability of the particle size, and the emulsion particle size cannot be dynamically regulated according to the process change. Aiming at the related technology, the design and development of an emulsifier and a colostrum preparation method thereof are needed to be designed and developed, and continuous regulation and control of the particle size of emulsified liquid drops can be realized by regulating the flow rates of a mobile phase and a disperse phase on the premise of not replacing a micro-pore plate, so that the single dependence of the traditional emulsifier on the aperture of the micro-pore plate is eliminated, and the production flexibility and the adaptability of equipment are obviously improved. Disclosure of Invention In order to get rid of the single dependence of the traditional emulsifier on the aperture of the microporous plate and remarkably improve the production flexibility and adaptability of equipment, the application provides a flow focusing type emulsifier. The application provides a flow focusing type emulsifier which adopts the following technical scheme: A flow focusing emulsifier comprising: The upper cover is fixedly connected with the valve seat in a detachable mode to form an emulsifier main body; The micro-pore plate is arranged between the upper cover and the valve seat, and is provided with at least one micro-pore channel for realizing focusing emulsification of a disperse phase and a mobile phase; the sealing piece is arranged between the upper cover and the valve seat and is used for realizing axial and radial sealing; At least one mobile phase inlet provided at a side portion of the valve seat or the upper cover for introducing a mobile phase; a disperse phase inlet arranged at the top of the upper cover or the valve seat and used for introducing a disperse phase; The mixed phase outlet is arranged at the bottom of the valve seat and is used for discharging emulsified mixed liquid; the mobile phase inlet and the disperse phase inlet are in non-coplanar intersection at the micropore plate to form a three-dimensional flow focusing structure, so that the mobile phase generates shearing force relative to the disperse phase to break the mobile phase to form liquid drops; The emulsifier body is configured to achieve continuous regulation of emulsion droplet size without replacing the microplate by adjusting the flow rate of the mobile phase and the dispersed phase. By adopting the technical scheme, the upper cover and the valve seat are fixedly connected in a detachable manner to form the main body of the emulsifier, and the micro-pore plate is arranged between the upper cover and the valve seat, so that the micro-pore plate is convenient to clean and replace, and the maintainability and the service life of the equipment are improved; At least one micropore channel is arranged on the micropore plate, so that focusing emulsification of a disperse phase and a mobile phase is realized, a sealing element is arranged between the upper cover and the valve seat, and the sealing element is matched with an axial and radial double sealing structure, so that the leakage-proof safety under a high-pressure working condition is ensured; The mobile phase inlet is arranged at the side part of the valve seat or the upper cover, the disperse phase inlet is arranged at the top of the upper cover or the valve seat, the mixed phase outlet is arranged at the bottom of the valve seat, the mobile phase inlet and the disperse phase inlet are in non-coplanar intersection at the micropore plate to form a three-dimensional flow focusing structure, so that th