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CN-122006543-A - Stirring paddle and bioreactor

CN122006543ACN 122006543 ACN122006543 ACN 122006543ACN-122006543-A

Abstract

The invention discloses a stirring paddle and a bioreactor, which relate to the technical field of bioengineering, wherein the stirring paddle comprises a stirring shaft, a plurality of first paddles, a connecting cylinder, a plurality of second paddles and two annular discs, wherein the plurality of first paddles are arranged at the circumferential side of the stirring shaft at intervals along the circumferential direction of the stirring shaft, the connecting cylinder is connected with one side end of each first paddle, which is far away from the stirring shaft, the plurality of second paddles are arranged at the circumferential side of the connecting cylinder at intervals along the axial direction of the stirring shaft, the two annular discs are respectively arranged at two side ends of the second paddles in the axial direction of the stirring shaft, and the inner rings of the two annular discs are connected with one side end of each first paddle, which is far away from the stirring shaft. The method effectively avoids the excessive shearing stress of the fluid at the edge of the blade, promotes the effective circulation and mixing of the fluid in the stirring paddle, can mildly and fully disturb the cell mass in the culture solution, effectively prevents the excessive self-agglomeration of the cells, improves the exchange efficiency of gas and nutrient in the mass, and promotes the discharge of metabolic waste.

Inventors

  • ZHANG YONGLIN

Assignees

  • 深圳市铂生生物智能装备有限公司

Dates

Publication Date
20260512
Application Date
20260123

Claims (10)

  1. 1. A stirring paddle, which comprises a stirring blade, characterized by comprising the following steps: A stirring shaft; the first paddles are arranged on the circumferential side of the stirring shaft at intervals along the circumferential direction of the stirring shaft; The connecting cylinder is connected with one side end of each first blade, which is away from the stirring shaft; the second paddles are arranged on the periphery of the connecting cylinder at intervals along the axial direction of the stirring shaft; The two circular ring discs are respectively arranged at two axial side ends of the second paddle on the stirring shaft, and the inner rings of the two circular ring discs are connected with one side end of the first paddle, which is away from the stirring shaft.
  2. 2. The stirring paddle of claim 1, wherein each of the first paddles is provided with a connecting plate between the two axial side ends of the stirring shaft and the inner rings of the two annular disks.
  3. 3. A stirring paddle as claimed in claim 2, wherein the web is located between the same lateral ends of adjacent two of the first paddles to close a gap between a portion of adjacent two of the first paddles.
  4. 4. A mixing paddle as recited in claim 2 wherein each of said first paddles has two webs disposed thereon.
  5. 5. The stirring paddle of claim 2 wherein the number of first paddles is at least four.
  6. 6. The stirring paddle of claim 1, wherein a plurality of connecting holes are formed in the circumferential side of the connecting cylinder, and each connecting hole is located between two adjacent second paddles.
  7. 7. The stirring paddle of claim 1, wherein a side end of the second paddle facing the stirring shaft is connected with the connecting cylinder, and a side end of the second paddle facing away from the stirring shaft is provided with an arc plate.
  8. 8. A bioreactor for the treatment of a biological material, characterized by comprising the following steps: A container; A stirring paddle comprising the stirring paddle of claims 1-7; The magnetic connection stirring transmission device is in transmission connection with the stirring paddle; And the driving device is in driving connection with the magnetic connection stirring transmission device so as to drive the stirring paddle to rotate.
  9. 9. The bioreactor of claim 8, wherein the drive means comprises at least one servo motor to drive the paddles to rotate clockwise or counterclockwise.
  10. 10. The bioreactor of claim 8, wherein the bottom of the vessel is arcuate.

Description

Stirring paddle and bioreactor Technical Field The invention relates to the technical field of bioengineering, in particular to a stirring paddle and a bioreactor. Background The carrier-free three-dimensional suspension cell culture requires low shear force cell culture, and domesticated adherent animal cells (such as HEK293 cells) can automatically agglomerate in the long-period culture process, so that the gas/component nutrient exchange and waste discharge in the cell growth process are not facilitated, and the cell activity is affected. In the whole culture period, the stirring rotation speed needs to be adjusted in real time according to the cell aggregation characteristic, and meanwhile, in the adjustment process, the shearing force requirement needs to be met in the whole process, so that the activity of cells is not influenced by overlarge shearing force and superposition. The conventional paddle-like bioreactor has the advantages that due to the characteristic of stirring, a better flow field can be provided under a relatively high linear speed, the requirement of cell suspension culture is met, the shearing force is exponentially amplified due to the high linear speed, and the low shearing force requirement of carrier-free three-dimensional suspension cell culture cannot be met. Disclosure of Invention The invention mainly aims to provide a stirring paddle and a bioreactor, which aim to realize uniform suspension of cells and reduce fluid shear force, thereby protecting the activity of the cells and improving the culture efficiency and stability. In order to achieve the above object, the stirring paddle according to the present invention comprises: A stirring shaft; the first paddles are arranged on the circumferential side of the stirring shaft at intervals along the circumferential direction of the stirring shaft; The connecting cylinder is connected with one side end of each first blade, which is away from the stirring shaft; the second paddles are arranged on the periphery of the connecting cylinder at intervals along the axial direction of the stirring shaft; The two circular ring discs are respectively arranged at two axial side ends of the second paddle on the stirring shaft, and the inner rings of the two circular ring discs are connected with one side end of the first paddle, which is away from the stirring shaft. Preferably, a connecting plate is arranged between the two axial side ends of the stirring shaft and the inner rings of the two annular disks. Preferably, the connecting plate is located between the same side ends of two adjacent first paddles to close a gap between two adjacent first paddles. Preferably, each of the first paddles is provided with two connecting plates. Preferably, the number of the first paddles is at least four. Preferably, a plurality of connecting holes are formed in the peripheral side of the connecting cylinder, and each connecting hole is located between two adjacent second paddles. Preferably, the second paddle faces towards one side end of the stirring shaft and is connected with the connecting cylinder, and one side end of the second paddle, which faces away from the stirring shaft, is provided with an arc plate. In addition, the invention also provides a bioreactor, which comprises a container, a stirring paddle, a magnetic connection stirring transmission device and a driving device, wherein the stirring paddle comprises a stirring shaft, a plurality of first paddles, a connecting cylinder, a plurality of second paddles and two circular discs, the plurality of first paddles are arranged on the circumferential side of the stirring shaft at intervals along the circumferential direction of the stirring shaft, the connecting cylinder is connected with one side end of each first paddle, which is far away from the stirring shaft, the plurality of second paddles are arranged on the circumferential side of the connecting cylinder at intervals along the axial direction of the stirring shaft, the two circular discs are respectively arranged on two axial side ends of the second paddles, the inner rings of the two circular discs are connected with one side end of each first paddle, which is far away from the stirring shaft, the magnetic connection stirring transmission device is in transmission connection with the stirring paddle, and the driving device is in driving connection with the magnetic connection stirring transmission device so as to drive the stirring paddle to rotate. Preferably, the driving means comprises at least one servo motor to drive the paddles to rotate clockwise or counter-clockwise. Preferably, the bottom of the container is arranged in an arc shape. According to the technical scheme provided by the invention, the plurality of first paddles are arranged at intervals on the circumferential side of the stirring shaft along the circumferential direction of the stirring shaft, the connecting cylinder is connected with one side end of each fir