CN-122006643-A - Reagent recovery fluid system

CN122006643ACN 122006643 ACN122006643 ACN 122006643ACN-122006643-A

Abstract

The invention discloses a reagent recovery fluid system. The reagent recovery fluid system comprises a reagent supply assembly, a synthesis assembly and a recovery assembly, wherein the reagent supply assembly comprises a reagent source, a liquid supply pipeline and a reagent driving pump, one end of the liquid supply pipeline is communicated with the reagent source, the reagent driving pump is arranged on the liquid supply pipeline, the synthesis assembly comprises a synthesis device and a waste discharge pipeline, the other end of the liquid supply pipeline is communicated with an inlet of a synthesis cavity of the synthesis device, one end of the waste discharge pipeline is communicated with a waste discharge port of the synthesis cavity, the recovery assembly comprises a first switching valve and a plurality of waste liquid recovery containers, the other end of the waste discharge pipeline is communicated with a liquid inlet of the first switching valve, and the waste liquid recovery containers are communicated with a plurality of liquid outlet positions of the first switching valve in a one-to-one correspondence manner. The reagent recovery fluid system can automatically switch the empty waste liquid recovery container when one waste liquid recovery container reaches a preset capacity, and the time for switching the waste liquid recovery container is short.

Inventors

SHEN YUE
LIANG CHENGLONG
ZHANG XIANDI
WANG KANGSHI
CAI JIANSHEN
ZHANG WENWEI

Assignees

深圳华大生命科学研究院

Dates

Publication Date: 20260512
Application Date: 20241111

Claims (10)

1. A reagent recovery fluid system, comprising: A reagent supply assembly (10), wherein the reagent supply assembly (10) comprises a reagent source (100), a liquid supply pipeline (200) and a reagent driving pump (300), one end of the liquid supply pipeline (200) is communicated with the reagent source (100), and the reagent driving pump (300) is arranged on the liquid supply pipeline (200); A synthesis assembly (20), wherein the synthesis assembly (20) comprises a synthesis device (400) and a waste discharge pipeline (500), the synthesis device (400) is provided with a synthesis cavity, the other end of the liquid supply pipeline (200) is communicated with an inlet of the synthesis cavity, and one end of the waste discharge pipeline (500) is communicated with a waste discharge port of the synthesis cavity; The recovery assembly (30), the recovery assembly (30) comprises a first switching valve (600) and a plurality of waste liquid recovery containers (700), the other end of the waste discharge pipeline (500) is communicated with a liquid inlet of the first switching valve (600), and the plurality of waste liquid recovery containers (700) are communicated at a plurality of liquid outlets of the first switching valve (600) in a one-to-one correspondence manner; The reagent recovery fluid system detects that the capacity in the waste liquid recovery container (700) reaches a preset capacity, and controls the first switching valve (600) to act so that the waste discharge flow in the first switching valve (600) is switched from the current waste liquid recovery container (700) to the empty waste liquid recovery container (700).
2. The reagent recovery fluid system of claim 1, wherein, The reagent amount of the single pumping of the reagent driving pump (300) is fixed, the reagent recovery fluid system can obtain the total amount of the pumped reagent according to the pumping times of the reagent driving pump (300), and the first switching valve (600) can be controlled to act according to the total amount of the reagent and the capacity of the waste liquid recovery container (700).
3. The reagent recovery fluid system of claim 1, wherein the liquid supply line (200) comprises a junction line (210) and at least two branch lines (220), one end of at least two of the branch lines (220) each communicating with one end of the junction line (210), the other end of the junction line (210) communicating with the inlet of the synthesis chamber; The reagent source (100) is provided with at least two, the other ends of the at least two branch pipelines (220) are respectively communicated with the at least two reagent sources (100) in a one-to-one correspondence manner, and each branch pipeline (220) is respectively provided with one reagent driving pump (300).
4. A reagent recovery fluid system according to claim 3, wherein at least one of a plurality of said reagent driven pumps (300) is operated, different ones of said reagent sources (100) containing the same or different reagents, said reagents comprising a reactive reagent and a cleaning reagent.
5. A reagent recovery fluid system according to claim 3, wherein at least two of the branch lines (220) are in communication with the junction line (210) via a multi-way junction.
6. The reagent recovery fluid system of claim 1, wherein the reagent driven pump (300) is a high pressure constant flow pump; Or the reagent driven pump (300) is a syringe dosage pump.
7. The reagent recovery fluid system of claim 1, wherein the synthesis device (400) is provided with a plurality; The synthesis assembly (20) further comprises a second switching valve (800), wherein a total inlet, a total outlet and a plurality of branch flow paths are arranged on the second switching valve (800), the total inlet is communicated with the other end of the liquid supply pipeline (200), the total outlet is communicated with the waste discharge pipeline (500), the flow path inlets of the plurality of branch flow paths can be selectively communicated with the total inlet, the flow path outlets of the plurality of branch flow paths can be selectively communicated with the total outlet, and the synthesis device (400) is respectively communicated with the plurality of branch flow paths in a one-to-one correspondence manner through a plurality of branch pipes (900).
8. The reagent recovery fluid system according to claim 7, wherein the branched flow path includes a first flow path having one end as the flow path inlet and the other end forming a first communication port in a valve housing of the second switching valve (800), and a second flow path having one end as the flow path outlet and the other end forming a second communication port in a valve housing of the second switching valve (800); The branch pipe (900) comprises a first pipe (910) and a second pipe (920), wherein the first pipe (910) is communicated between an inlet of the synthesis cavity and the first communication port, and the second pipe (920) is communicated between a waste outlet of the synthesis cavity and the second communication port.
9. The reagent recovery fluid system of claim 1, wherein the waste line (500) is provided with a restrictor valve (510).
10. The reagent recovery fluid system of any one of claims 1-9, wherein the reagent source (100) comprises a plurality of reagent containers, different ones of the reagent containers containing the same or different reagents.

Description

Reagent recovery fluid system Technical Field The invention relates to the technical field of nucleic acid synthesis, in particular to a reagent recovery fluid system. Background The nucleic acid synthesis system is a device for synthesizing nucleic acid by a chemical method, and in the nucleic acid synthesis system, a waste liquid recovery tank is a key component of the nucleic acid synthesis system for recovering a reagent waste liquid generated in the nucleic acid synthesis process. In the existing nucleic acid synthesis process, only one waste liquid recycling bin is generally arranged for receiving all reagent waste liquid, and whether the reagent waste liquid of the waste liquid recycling bin is full or not is generally judged through manual observation and experience, after the waste liquid recycling bin is judged to be full, the waste liquid recycling bin is manually detached for dumping the reagent waste liquid, and after the waste liquid recycling bin is emptied, the waste liquid recycling bin is reassembled for collecting the reagent waste liquid. The method has the advantages that whether the waste liquid recycling bin is full of liquid is judged manually by experience, the accuracy is low, the stability is poor, the operation of the method for removing the waste liquid recycling bin to pour reagent waste liquid is complex, a large amount of manpower is occupied, the safety is poor, the nucleic acid synthesis reaction needs to be suspended, the working efficiency of the device is seriously reduced, the reaction duration of the nucleic acid synthesis reaction is not easy to control, and the effect of the nucleic acid synthesis reaction is poor. Particularly for large-scale connected nucleic acid synthesis reactions, a large amount of reagent waste liquid is generated, so that the waste liquid recycling bin needs to be frequently disassembled to avoid the overflow of the reagent waste liquid from the waste liquid recycling bin, and the defect of the way of manually disassembling and pouring the reagent waste liquid is further amplified, so that the use requirement cannot be met. In addition, because all the nucleic acid synthesis reactions use chemical reagents, the nucleic acid synthesis reaction has the characteristics of strong corrosiveness, inflammability, explosiveness and the like, a liquid level sensor cannot be arranged in the waste liquid recovery barrel to monitor whether the reagent waste liquid in the waste liquid recovery barrel is full. Therefore, how to provide a recovery system that can monitor the amount of the reagent waste liquid in real time and improve the recovery efficiency of the reagent waste liquid to avoid occupying a large amount of time due to dumping the reagent waste liquid is a technical problem that needs to be solved at present. Disclosure of Invention The invention aims to provide a reagent recovery fluid system which can monitor the reagent waste liquid amount in real time and can quickly replace a waste liquid recovery container for collecting the reagent waste liquid. To achieve the purpose, the invention adopts the following technical scheme: A reagent recovery fluid system comprises a reagent supply assembly, a synthesis assembly and a recovery assembly, wherein the reagent supply assembly comprises a reagent source, a liquid supply pipeline and a reagent driving pump, one end of the liquid supply pipeline is communicated with the reagent source, the reagent driving pump is arranged on the liquid supply pipeline, the synthesis assembly comprises a synthesis device and a waste liquid recovery pipeline, the synthesis device is provided with a synthesis cavity, the other end of the liquid supply pipeline is communicated with an inlet of the synthesis cavity, one end of the waste liquid discharge pipeline is communicated with a waste discharge port of the synthesis cavity, the recovery assembly comprises a first switching valve and a plurality of waste liquid recovery containers, the other end of the waste liquid recovery pipeline is communicated with a liquid inlet of the first switching valve, the plurality of waste liquid recovery containers are communicated at a plurality of liquid outlets of the first switching valve in a one-to-one correspondence mode, the reagent recovery fluid system detects that the capacity in the waste liquid recovery containers reaches a preset capacity, and controls the first switching valve to act so that the waste liquid discharge flow paths in the first switching valve are switched to the empty recovery containers of the waste liquid in the current switching containers. Preferably, the reagent-driven pump has a fixed amount of reagent to be pumped at a single time, and the reagent recovery fluid system can obtain the total amount of reagent to be pumped according to the pumping frequency of the reagent-driven pump, and can control the first switching valve to operate according to the total amount of reagent and the capacity of t