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CN-122006826-A - Calibration method and calibration system for working position of detection card box

CN122006826ACN 122006826 ACN122006826 ACN 122006826ACN-122006826-A

Abstract

The invention provides a calibration method and a calibration system for a working position of a detection card box, the detection card box comprises a box body and a rotary valve, the box body is provided with a rotary cavity and a plurality of functional cavities, the box body is provided with a plurality of liquid passing holes which are correspondingly communicated with the functional cavities, the rotary valve is provided with a rotary disk, a liquid moving cavity is arranged in the rotary disk, the rotary disk is provided with liquid moving holes which are communicated with the liquid moving cavity, the liquid moving holes are sequentially communicated with the liquid passing holes through the rotary disk, the calibration method comprises the steps of obtaining flow change data of the liquid moving holes or the liquid moving cavities through a flow detection element while the rotary valve rotates at a uniform speed, determining displacement data generated when the rotary valve is communicated with the liquid passing holes from an initial position according to the flow change data, and calibrating the working position of the corresponding liquid passing hole according to the displacement data. The invention can automatically and accurately mark the working position of the rotary valve in the detection cartridge at each step so as to ensure that the detection cartridge with high precision requirement can work stably and reliably.

Inventors

  • LU HONGYU
  • LI JIONG
  • MA YONGYUE

Assignees

  • 鲲鹏(徐州)科学仪器有限公司
  • 鲲鹏基因(北京)科学仪器有限公司

Dates

Publication Date
20260512
Application Date
20251231

Claims (12)

  1. 1. A calibration method for detecting the working position of a card box is characterized in that, The detection cartridge includes: The box body is provided with a rotating cavity and a plurality of functional cavities surrounding the periphery of the rotating cavity, the box body is provided with a plurality of liquid passing holes, and the liquid passing holes are respectively communicated with the corresponding functional cavities; The rotary valve is provided with a rotary disk, a pipetting chamber is arranged in the rotary disk, pipetting holes communicated with the pipetting chamber are formed in the rotary disk, and the rotary disk rotates to enable the pipetting holes to be communicated with the through holes in sequence along the rotation direction; the calibration method comprises the following steps: The rotary valve rotates at a constant speed, and meanwhile, flow change data of the pipetting hole or the pipetting chamber are obtained through a flow detection element; According to the flow change data, determining displacement data generated when the rotary valve is communicated with the liquid passing hole from an initial position to the liquid moving hole; and calibrating the corresponding working position of the liquid passing hole according to the displacement data.
  2. 2. The method for calibrating an operating position of a test cartridge according to claim 1, wherein before the rotary valve rotates at a constant speed, the method further comprises: an initial position of the rotary valve is preset, the flow detection element is started, and air is injected into the pipetting hole or the pipetting chamber until a detection value of the flow detection element is reset to zero.
  3. 3. The method for calibrating an operation position of a detection cartridge according to claim 1, wherein determining displacement data generated from the initial position to when the pipetting hole is in communication with the through-hole according to the flow rate variation data comprises: determining a time interval required for the flow change data to be restored to zero from zero to the maximum according to the flow change data; Acquiring a time median value of the time interval, wherein the time median value is the moment when the pipetting holes are aligned and communicated with the through holes; And determining displacement data generated when the rotary valve rotates to align and communicate the liquid transferring hole with the liquid passing hole according to the time median.
  4. 4. The method for calibrating the working position of the detection cartridge according to claim 3, wherein the detection cartridge further comprises a driving motor, and an output shaft of the driving motor is connected with the rotary valve so as to drive the rotary valve to rotate at a constant speed; the displacement data generated when the rotary valve rotates to the state that the pipetting hole is aligned with the through hole comprises the rotating angle of the rotary valve or the step length of the driving motor.
  5. 5. The method of calibrating an operating position of a test cartridge according to claim 1, wherein the test cartridge further comprises a flow detection element for detecting flow change data at the pipetting orifice or the pipetting chamber and/or injecting fluid into the pipetting orifice or the pipetting chamber.
  6. 6. The method for calibrating an operating position of a cartridge according to any one of claims 1 to 5, wherein an area of the pipetting hole is larger than or equal to an area of the through-hole.
  7. 7. The method of calibrating an operating position of a cartridge according to claim 6, wherein the pipetting holes and the through holes have the same shape.
  8. 8. The method for calibrating a working position of a detection cartridge according to claim 1, wherein the rotary cavity is located at an intermediate position inside the cartridge body, and a plurality of functional cavities are circumferentially arranged around the periphery of the rotary cavity at intervals along the periphery of the rotary cavity; the liquid through holes are positioned on the bottom wall of the box body, and are communicated with the functional cavities in a one-to-one correspondence manner.
  9. 9. The method for calibrating a working position of a detection cartridge according to claim 1 or 8, wherein the rotating disc is rotatably arranged below the cartridge body, and the pipetting chamber is directly communicated with the rotating chamber; the liquid transferring holes are formed in the top surface of the rotating disc, and when the rotating disc rotates to the position where the liquid transferring holes are staggered with the liquid passing holes, the top surface of the rotating disc clings to the bottom wall of the box body so as to seal the liquid transferring holes through the bottom wall of the box body.
  10. 10. The method for calibrating the working position of the detection card box according to claim 1 or 8, wherein the rotary valve is further provided with a rotary shaft, the bottom end of the rotary shaft is connected with the top center position of the rotary disk, the rotary disk is rotatably arranged below the box body, the rotary shaft penetrates through the rotary cavity from bottom to top, a hollow cavity is formed in the rotary shaft, and the hollow cavity is communicated with the pipetting cavity; the liquid transferring holes are formed in the top surface of the rotating disc, and when the rotating disc rotates to the position where the liquid transferring holes are staggered with the liquid passing holes, the top surface of the rotating disc clings to the bottom wall of the box body so as to seal the liquid transferring holes through the bottom wall of the box body.
  11. 11. The method of calibrating a working position of a cartridge according to claim 10, wherein the rotating shaft is integrally formed with the rotating disc.
  12. 12. A calibration system for detecting the working position of a cartridge, which performs calibration of the working position by using the calibration method according to any one of claims 1 to 11, characterized in that the calibration system comprises: The test cartridge of any one of claims 1 to 11; A flow detection element for detecting a change in flow at a pipetting hole or chamber in the detection cartridge and/or injecting fluid into the pipetting hole or chamber; And the driving motor is used for driving the rotary valve in the detection card box to rotate at a constant speed.

Description

Calibration method and calibration system for working position of detection card box Technical Field The invention relates to the technical field of detection kits, in particular to a calibration method and a calibration system for the working position of a detection kit, and particularly relates to a calibration method and a calibration system for the working position of a nucleic acid detection kit. Background Microfluidic detection cartridges, in particular cartridges for nucleic acid detection, typically employ a rotary valve as their core fluid control element. In the chinese patent application entitled "reagent vessel for nucleic acid extraction and nucleic acid amplification" with application number 202510486993.6, a micro flow channel is provided in the rotary valve (i.e., flow channel switching valve), and a through hole communicating with the micro flow channel is provided on the rotary valve, and the through hole communicating with the micro flow channel on the valve body is matched with a plurality of liquid passing holes (wherein, different liquid passing holes can be respectively used as functional holes such as sample holes, cleavage holes, washing holes, reaction holes) on the box body, so as to realize the alignment communication of the micro flow channel and different liquid passing holes. In the actual working process, through driving the valve body to rotate step by step, the through holes on the valve body can be aligned with different through holes on the box body in sequence, so that sequential transfer and reaction of liquid such as a sample, a pyrolysis liquid, a washing liquid, an amplification reagent and the like among different closed cavities in the box body are realized. Therefore, the rotary positioning accuracy of the rotary valve directly determines whether the fluid passage can be reliably and stably communicated, which is a key for ensuring whether the whole nucleic acid detection process can be smoothly carried out and ensuring the accuracy of results. Currently, the rotation control of rotary valves in the prior art mainly depends on the following two ways: The first is a fixed angle stepping method, which is based on the angular difference of fixed central angles between the functional holes on the box body. Fixed stepping angles corresponding to these angle differences are preset in the control program, and the drive motor rotates in accordance with a preset stepping angle sequence (for example, the drive motor rotates 30 ° or 45 ° each time, etc.). However, this method has inherent drawbacks in that since the cartridge body inevitably has manufacturing tolerances and assembly clearances during injection molding and assembly of the cartridge body and the rotary valve, there is a slight deviation of the theoretical rotation center of the rotary valve from the actual circle center of circumferential distribution of the functional holes on the cartridge body, and such concentricity deviation may cause the through-holes on the rotary valve to be completely and precisely aligned with the functional holes on the cartridge body even if the driving motor can precisely perform rotation by a preset fixed angle, thereby causing a positioning error. The second is a visual or sensor auxiliary judgment method, which judges the alignment state of the through hole on the rotary valve and the functional hole on the box body by arranging marks on the box body and/or the rotary valve and identifying the preset marks through an optical sensor or a visual system and the like. Although the method can compensate static assembly errors in the first method to a certain extent, the configuration of the optical sensor or the vision system is more complex and the cost is greatly increased, and more importantly, when the optical sensor or the vision system is used for a long time or is affected by vibration, temperature change and the like, the optical sensor or the vision system also has the problems of position drift, mark pollution, abrasion and the like, a new error source is introduced, and further, the judgment misalignment is caused. It can be seen that, in both the above-mentioned "open loop" control relying on fixed angle stepping and the "closed loop" feedback relying on an external sensor, it is difficult in the prior art to always ensure long-term, stable and high-precision alignment of the through hole on the rotary valve and the functional hole on the box body in the whole life cycle of the detection cartridge, and the deviation of this alignment may cause situations of unsmooth liquid transfer, residues, cross contamination or complete blockage of the flow channel, which may seriously affect the reliability of the detection process and the accuracy of the experimental result. Therefore, the invention provides a calibration method and a calibration system for the working position of a detection card box, so as to overcome the defects of the prior art. Disclosure of Invention The inv