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CN-122006828-A - Center radiation type integrated three-dimensional high-flux microfluidic chip and liquid drop preparation method

CN122006828ACN 122006828 ACN122006828 ACN 122006828ACN-122006828-A

Abstract

The invention discloses a central radiation type integrated three-dimensional high-flux microfluidic chip and a liquid drop preparation method, which belong to the technical field of microfluidics and comprise a lower liquid inlet plate, a middle functional plate and an upper packaging plate, wherein the lower liquid inlet plate, the middle functional plate and the upper packaging plate are sequentially distributed from bottom to top, the lower liquid inlet plate for introducing continuous phase and disperse phase and vertically conveying the middle functional plate is fixedly connected with the middle functional plate, the middle functional plate for distributing the continuous phase and the disperse phase, generating liquid drops and collecting and outputting the liquid drops is fixedly connected with the upper packaging plate for sealing a middle flow channel and forming a packaging cavity, the lower liquid inlet plate is connected with a liquid inlet component, and the middle functional plate is connected with a liquid outlet component. By the method, multichannel parallel liquid drop generation can be realized on the premise of ensuring the uniformity of the liquid drop size, so that the overall working efficiency of the microfluidic liquid drop generation system is effectively improved.

Inventors

  • Hao nanjing
  • HONG XUAN
  • MA LI
  • XUE SHUYUAN
  • CHEN ZHENZHEN

Assignees

  • 西安微化精工科技有限公司

Dates

Publication Date
20260512
Application Date
20260403

Claims (8)

  1. 1. The utility model provides a three-dimensional high flux micro-fluidic chip of integrated of central radiation formula, includes lower floor's feed liquor board (1), middle level function board (2) and upper packaging board (3), its characterized in that: The lower-layer liquid inlet plate (1), the middle-layer functional plate (2) and the upper-layer packaging plate (3) are sequentially distributed from bottom to top, the lower-layer liquid inlet plate (1) for introducing a continuous phase and a disperse phase and vertically conveying the middle-layer functional plate (2) is fixedly connected with the middle-layer functional plate (2), the middle-layer functional plate (2) for distributing the continuous phase and the disperse phase, generating liquid drops and collecting and outputting the liquid drops is fixedly connected with the upper-layer packaging plate (3) for sealing a middle-layer runner and forming a packaging cavity, a liquid inlet component is connected onto the lower-layer liquid inlet plate (1), a liquid outlet component is connected onto the middle-layer functional plate (2), and the liquid inlet component is connected with the liquid outlet component; The liquid inlet component comprises a disperse phase liquid inlet unit and a continuous phase liquid inlet unit, and the disperse phase liquid inlet unit and the continuous phase liquid inlet unit are connected with the lower liquid inlet plate (1).
  2. 2. The integrated three-dimensional high-flux microfluidic chip according to claim 1, wherein the dispersed phase liquid inlet unit comprises a dispersed phase needle tube connecting channel (11), a lower dispersed phase fluid channel (12) and a lower dispersed phase vertical channel (13), the dispersed phase needle tube connecting channel (11), the lower dispersed phase fluid channel (12) and the lower dispersed phase vertical channel (13) are fixedly connected to the upper end of the lower liquid inlet plate (1), the discharge end of the dispersed phase needle tube connecting channel (11) is fixedly connected and communicated with the feed end of the lower dispersed phase fluid channel (12), the discharge end of the lower dispersed phase fluid channel (12) is fixedly connected and communicated with the feed end of the lower dispersed phase vertical channel (13), the lower dispersed phase vertical channel (13) is provided with a plurality of groups, and the discharge end of the lower dispersed phase vertical channel (13) is connected with the liquid outlet assembly.
  3. 3. The integrated three-dimensional high-flux microfluidic chip according to claim 2, wherein the continuous phase liquid inlet unit comprises a continuous phase needle tube connecting channel (16), a lower continuous phase fluid channel (15) and a lower continuous phase vertical channel (14), the continuous phase needle tube connecting channel (16), the lower continuous phase fluid channel (15) and the lower continuous phase vertical channel (14) are fixedly connected to the upper end of the lower liquid inlet plate (1), the discharge end of the continuous phase needle tube connecting channel (16) is fixedly connected and communicated with the feed end of the lower continuous phase fluid channel (15), the discharge end of the lower continuous phase fluid channel (15) is fixedly connected and communicated with the feed end of the lower continuous phase vertical channel (14), and the discharge end of the lower continuous phase vertical channel (14) is connected with the liquid outlet assembly.
  4. 4. The integrated three-dimensional high-flux microfluidic chip according to claim 3, wherein the liquid outlet component comprises a liquid drop collecting needle tube connecting channel (21), a liquid drop collecting channel (22), a middle-layer disperse phase vertical channel (24), a middle-layer continuous phase vertical channel (25), a middle-layer disperse phase fluid channel (26), a middle-layer continuous phase fluid channel (27) and a liquid drop generating channel (28), the liquid drop collecting needle tube connecting channels (21) are provided with two groups, and the two groups of liquid drop collecting needle tube connecting channels (21) distributed in bilateral symmetry are respectively fixedly connected to the left side and the right side of the middle-layer functional board (2); The feeding end of the liquid drop collecting needle tube connecting channel (21) is fixedly connected with and communicated with the discharging ends of the liquid drop collecting channels (22), the feeding end of each liquid drop collecting channel (22) is fixedly connected with and communicated with the discharging end of the liquid drop generating channel (28), the feeding end of the liquid drop generating channel (28) is a liquid drop generating unit (23), the liquid drop generating unit (23) is respectively fixedly connected with and communicated with the discharging ends of the middle-layer continuous phase fluid channel (27) and the middle-layer dispersed phase fluid channel (26), the discharging end of each middle-layer dispersed phase vertical channel (24) is fixedly connected with and communicated with the feeding end of the liquid drop generating unit (23), the feeding end of each middle-layer dispersed phase vertical channel (24) is fixedly connected with and communicated with the discharging end of the lower-layer dispersed phase vertical channel (13), and the discharging end of the middle-layer continuous phase vertical channel (25) is fixedly connected with and communicated with the discharging end of the lower-layer continuous phase vertical channel (14).
  5. 5. The integrated three-dimensional high-throughput microfluidic chip of claim 4, wherein the lower discrete phase vertical channels (13) have four groups, the four groups of lower discrete phase vertical channels (13) are circumferentially distributed along the center of the chip, the lower continuous phase vertical channels (14) are located at the geometric center of the chip, the lower continuous phase vertical channels (14) are used for conveying continuous phases from the lower central region to the middle central region, and the middle discrete phase vertical channels (24) are the same in number and in one-to-one correspondence with the groups of lower discrete phase vertical channels (13).
  6. 6. The integrated three-dimensional high-flux microfluidic chip of claim 4, wherein the middle continuous phase vertical channel (25) is located at the geometric center of the middle functional board (2), a central cavity is formed at the middle continuous phase vertical channel (25) of the middle functional board (2), the central cavity is a cylindrical cavity, and the diameter of the central cavity is larger than that of the middle continuous phase vertical channel (25).
  7. 7. The integrated three-dimensional high-throughput microfluidic chip of claim 4, wherein the number of sets of the droplet collecting channel (22), the droplet generating unit (23), the middle-stage dispersion phase fluid channel (26) and the middle-stage continuous phase fluid channel (27) is the same and corresponds one to one, and each of the droplet collecting channel (22), the droplet generating unit (23), the middle-stage dispersion phase fluid channel (26) and the middle-stage continuous phase fluid channel (27) has eight sets.
  8. 8. A method of droplet preparation utilizing the central radial integrated three-dimensional high-throughput microfluidic chip of claim 7, comprising the steps of: Injecting disperse phase fluid into a lower liquid inlet plate (1) through a disperse phase needle tube connecting channel (11), enabling the disperse phase fluid to reach four groups of lower disperse phase vertical channels (13) through a lower disperse phase fluid channel (12), enabling the disperse phase fluid to enter a middle disperse phase vertical channel (24) in a middle functional plate (2) through a corresponding lower disperse phase vertical channel (13), and then, further dividing the disperse phase fluid into two paths through each middle disperse phase vertical channel (24), and respectively conveying the two paths to a liquid drop generating unit (23) through a middle disperse phase fluid channel (26); Injecting continuous phase fluid into the lower liquid inlet plate (1) through the continuous phase needle tube connecting channel (16) at the same time, enabling the continuous phase fluid to reach a lower continuous phase vertical channel (14) positioned at the center of a chip through a lower continuous phase fluid channel (15), and enter a middle continuous phase vertical channel (25) in the middle functional plate (2) through the lower continuous phase vertical channel (14), then enabling the continuous phase fluid to enter a central cavity on the middle functional plate (2) firstly, then distributing the continuous phase fluid into eight groups of middle continuous phase fluid channels (27) through the central cavity, and respectively conveying the continuous phase fluid to eight groups of liquid drop generating units (23) through the eight groups of middle continuous phase fluid channels (27); Step three, in each group of liquid drop generating units (23), the disperse phase contacts with the continuous phase in a step-T intersection area, and liquid drops are formed under the shearing action of the continuous phase; And step four, the generated liquid drops are collected through a liquid drop collecting channel (22) and output to an external collecting device from a left liquid drop collecting needle tube connecting channel (21) and a right liquid drop collecting needle tube connecting channel.

Description

Center radiation type integrated three-dimensional high-flux microfluidic chip and liquid drop preparation method Technical Field The invention relates to the technical field of microfluidics, in particular to a central radiation type integrated three-dimensional high-flux microfluidic chip and a liquid drop preparation method. Background Microfluidic technology is a technology system that performs transport, distribution and reaction control of fluids in micrometer-scale channels. Droplet microfluidics is an important branch of microfluidic technology, and by forming discrete droplets from two-phase fluids which are mutually insoluble in a microchannel, a high parallel operation mode of a single droplet, namely a reactor, is realized, and droplet generation is mainly divided into an active type and a passive type. The existing passive droplet generation mode generally depends on shear and interfacial instability mechanisms of a disperse phase and a continuous phase at a specific geometric structure, and the droplet size and the generation frequency are controlled by adjusting parameters such as flow, viscosity, interfacial tension, channel geometric dimension and the like. In order to meet the demands of practical applications for processing efficiency, droplet microfluidic systems often need to increase droplet throughput. When the flow rate or pressure of the single droplet generation unit is increased to pursue higher generation frequency, the phenomenon of fluctuation of droplet size, satellite droplet generation, flow pattern change and the like may occur, so that the droplet monodispersity and the operation stability are affected. Thus, engineering implementations that increase throughput generally have a greater tendency to extend capacity by multiple units in parallel to increase overall throughput while maintaining relatively stable unit operating conditions. For example, chinese patent CN121467127a discloses a liquid-separating-converging integrated droplet microfluidic chip and a droplet preparation method, in which a droplet generating unit is disposed at a connection position between a branch splitting channel and a converging channel which are correspondingly connected, and the continuous phase solution and the dispersed phase solution are sheared by the continuous phase solution due to differences of viscosity and interfacial tension of the continuous phase solution and the shearing force of the continuous phase, so as to form droplets. The liquid drop generating units are symmetrically distributed along the circumference of the channel, the distances from each unit to the center are equal, and the continuous phase solution and the disperse phase solution which meet at each liquid drop generating unit are almost equivalent, so that the generated liquid drops are ensured to be consistent in size, the liquid drops with basically consistent sizes are obtained at the collecting port, and the high-flux preparation of the microspheres with uniform sizes is realized. The invention has the following defects that the invention is of a single-layer plane integrated structure, the continuous communication channels need to complete multistage splitting, converging and re-splitting at the periphery of a disperse phase circumference distribution structure, so that the planar flow channels are more complicated to arrange, the continuous flow channels are longer obviously, and more turns are formed, and larger flow resistance and processing design complexity are easier to bring, meanwhile, the scheme relies on a large number of in-plane splitting-converging-re-splitting channels to realize multi-point liquid supply, and the aim is to ensure that the paths from each branch to a liquid drop generating unit are equal, but the problem that the local resistances of different branches are not completely consistent easily occurs in the actual structure realization, thereby affecting the fluid distribution uniformity. The short plates of the single-layer liquid-separating and converging scheme mainly show the problems of complex structure, long planar wiring, large flow resistance, difficult further optimization of branch distribution uniformity, more external collecting interfaces and the like. For example, chinese patent CN121372539a discloses an integrated droplet microfluidic chip and a droplet preparation method based on 3D printing, which includes a continuous phase channel unit and a dispersion channel unit, wherein the continuous phase channel unit includes a main channel unit, a plurality of main splitting channels are circumferentially communicated around an outlet of the main channel unit, the dispersion channel unit includes a branch channel unit, a plurality of branch splitting channels are circumferentially communicated around an outlet of the branch channel unit, the main splitting channel is a droplet control structure at a position where the main splitting channel is communicated with the outlet of