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CN-122006832-A - Multifunctional pipetting device and pipetting method

CN122006832ACN 122006832 ACN122006832 ACN 122006832ACN-122006832-A

Abstract

The invention relates to the technical field of pipetting devices, in particular to a multifunctional pipetting device and a pipetting method, wherein the multifunctional pipetting device comprises a pipetting gun nozzle, a small-capacity cylinder barrel, a small-capacity piston rod, a small-capacity pipetting mechanism, a large-capacity cylinder barrel, a large-capacity piston rod, a large-capacity pipetting mechanism, a small-capacity suction head, a large-capacity suction head and a filtering suction head, the pipetting gun nozzle comprises a small-capacity sleeve head part used for being matched with the small-capacity suction head and a large-capacity sleeve head part used for being matched with the large-capacity suction head or the filtering suction head, a filter screen is arranged in the filtering suction head, when the small-capacity suction head and the filtering suction head are sleeved on the pipetting gun nozzle, the bottom of the small-capacity suction head is positioned above the filter screen, and the multifunctional pipetting device can be well suitable for the situation that the two suction heads with different specifications are required to be matched for pipetting, and the two different measuring procedures are required to be used for pipetting, and the two large functions of pipetting and filtering are integrated into a whole.

Inventors

  • LUO GENGRONG
  • LIU LEIFENG

Assignees

  • 佛山市顺德区罗恩科学仪器有限公司

Dates

Publication Date
20260512
Application Date
20260317

Claims (9)

  1. 1. A multifunctional pipetting device is characterized by comprising a pipetting gun nozzle (2), a small-capacity cylinder barrel (3) communicated with the pipetting gun nozzle (2), a small-capacity piston rod (4) in the small-capacity cylinder barrel (3), a small-capacity pipetting power mechanism (5) for driving the small-capacity piston rod (4) to slide, a large-capacity cylinder barrel (6) communicated with the pipetting gun nozzle (2), a large-capacity piston rod (7) in the large-capacity cylinder barrel (6) in a sliding manner, a large-capacity pipetting power mechanism (8) for driving the large-capacity piston rod (7) to slide, a small-capacity suction head (14), a large-capacity suction head (15) and a filtering suction head (16), wherein the pipetting gun nozzle (2) comprises a small-capacity sleeve head (21) for being matched with the small-capacity suction head (14), and a large-capacity sleeve head (22) for being matched with the large-capacity suction head (15) or the filtering suction head (16), a filter screen (17) is arranged in the filtering suction head (16), and the small-capacity pipette nozzle (14) and the filtering suction head (16) is sleeved on the bottom of the small-capacity pipette nozzle (2) when the small-capacity suction head (14) is sleeved on the pipetting gun nozzle (2).
  2. 2. The multifunctional pipetting device according to claim 1, further comprising a pipetting integrated seat (1), wherein the small-capacity cylinder barrel (3), the small-capacity pipetting mechanism (5), the large-capacity cylinder barrel (6) and the large-capacity pipetting mechanism (8) are respectively arranged on the pipetting integrated seat (1), an output end of the small-capacity pipetting mechanism (5) is in driving connection with a small-capacity piston rod (4), and an output end of the large-capacity pipetting mechanism (8) is in driving connection with a large-capacity piston rod (7).
  3. 3. The multifunctional pipetting device according to claim 2, further comprising a transfer mechanism (9), wherein the pipetting integrated base (1) is connected with the end of the transfer mechanism (9).
  4. 4. A multi-purpose pipetting device as recited in claim 1 further comprising a tip holder (10), wherein the tip holder (10) is provided with a plurality of small-capacity receptacles (101) for receiving small-capacity tips (14), and a plurality of large-capacity receptacles (102) for receiving large-capacity tips (15) or filter tips (16).
  5. 5. The multi-functional pipetting device according to claim 1, further comprising a suction head holder (11), wherein the suction head holder (11) is formed with a small-capacity notch portion (111) for allowing a small-capacity suction head (14) to extend in the left-right direction, small-capacity flange portions (112) formed to protrude in the front-rear direction at the front-rear ends of the small-capacity notch portion (111), large-capacity notch portions (113) for allowing a large-capacity suction head (15) or a filter suction head (16) to extend in the left-right direction, and large-capacity flange portions (114) formed to protrude in the front-rear direction at the front-rear ends of the large-capacity notch portions (113).
  6. 6. A multi-purpose pipetting device as recited in claim 5 further comprising a tip collection box (12) disposed below the low volume rim portion (112) and the high volume rim portion (114).
  7. 7. A multi-functional pipetting device according to claim 1, wherein the small-volume sleeving part (21) and the large-volume sleeving part (22) are coaxially arranged, and the small-volume sleeving part (21) is positioned at the bottom of the large-volume sleeving part (22).
  8. 8. The multifunctional pipetting device according to claim 1, further comprising an air pipe (13), wherein the small-capacity cylinder barrel (3) and the large-capacity cylinder barrel (6) are arranged side by side along the front-back direction, the pipetting gun nozzle (2) is arranged at the bottom of the small-capacity cylinder barrel (3) and is directly communicated with the small-capacity cylinder barrel (3), and the large-capacity cylinder barrel (6) is communicated with the pipetting gun nozzle (2) through the air pipe (13).
  9. 9. A multifunctional pipetting method is characterized in that the multifunctional pipetting device based on any one of claims 1-8 comprises a small-capacity pipetting mode, a large-capacity pipetting mode and a filtration pipetting mode; The small-volume pipetting mode comprises the following steps: the small-capacity suction head (14) is sleeved on a small-capacity sleeve head (21) of the pipette nozzle (2), the small-capacity piston rod (4) is driven to slide upwards through the small-capacity pipette power mechanism (5) to absorb the small-capacity reagent, and the small-capacity piston rod (4) is driven to slide downwards through the small-capacity pipette power mechanism (5) to drain the small-capacity reagent; the high-capacity pipetting mode comprises the following steps: the high-capacity suction head (15) is sleeved on a high-capacity sleeve head (22) of the pipette nozzle (2), a high-capacity piston rod (7) is driven to slide upwards through a high-capacity pipetting mechanism (8) to suck high-capacity reagent, and the high-capacity piston rod (7) is driven to slide downwards through the high-capacity pipetting mechanism (8) to drain the high-capacity reagent; the filtration pipetting mode comprises the following steps: The method comprises the steps of firstly sleeving a small-capacity suction head (14) on a small-capacity sleeve head (21) of a pipette nozzle (2), driving a small-capacity piston rod (4) to slide upwards through a small-capacity hydraulic mechanism (5) and sucking an original sample through the small-capacity suction head (14), sleeving a filtering suction head (16) on a large-capacity sleeve head (22) of the pipette nozzle (2), driving the small-capacity piston rod (4) to slide downwards through the small-capacity hydraulic mechanism (5), and enabling the original sample positioned in the small-capacity suction head (14) to flow through a filter screen (17) of the filtering suction head (16) and then discharging.

Description

Multifunctional pipetting device and pipetting method Technical Field The invention relates to the technical field of pipetting devices, in particular to a multifunctional pipetting device and a pipetting method. Background Pipetting devices are key tools for accurate transfer of liquids in laboratory fields such as biology, chemistry, medical treatment, food detection, and the like. The conventional pipetting devices are all single-suction-head-specification pipetting devices, and comprise a cylinder barrel, a piston rod which is connected in a sliding manner in the cylinder barrel, and a pipetting mechanism for driving the piston rod to slide, wherein the pipetting device can only be used for being matched with a suction head of a single specification, can only be used for pipetting a single measuring range, cannot be suitable for the situation that the pipetting device needs to be matched with two suction heads of different specifications and is used for pipetting two different measuring ranges, has certain limitation, in addition, when pipetting operation needing to be filtered is carried out, samples are usually sucked out from an original container through the conventional pipetting device, then the samples are discharged into a filter tube with a filtering function, and filtered filtrate is sucked out from the filter tube through another new pipetting head and then transferred into a target container such as a detection plate or a reaction tube, so that the whole process is more tedious and lower in efficiency, the samples need to be transferred for multiple times, and the risks of sample pollution and sample loss are increased. Disclosure of Invention The invention aims to provide a multifunctional pipetting device aiming at the defects in the prior art, can realize double-range composite pipetting, can perform small-capacity pipetting or large-capacity pipetting switching according to specific use requirements, can be well suitable for the situations that two pipetting heads with different specifications are required to be matched for use and two pipetting heads with different ranges are required to be used for pipetting, has stronger overall flexibility and practicability, integrates pipetting and filtration, not only remarkably simplifies operation steps, improves efficiency, but also can reduce the number of times of sample transfer, thereby reducing the risk of sample pollution and the possibility of sample loss; The invention also provides a multifunctional pipetting method which not only has three pipetting modes and can adapt to diversified experimental requirements, but also compared with the traditional filtration pipetting mode, the filtration pipetting mode of the invention not only remarkably simplifies operation steps and improves efficiency, but also can reduce the times of sample transfer, thereby reducing the risk of sample pollution and the possibility of sample loss. In order to achieve the purpose, the multifunctional pipetting device comprises a pipetting gun nozzle, a small-capacity cylinder barrel communicated with the pipetting gun nozzle, a small-capacity piston rod slidingly connected in the small-capacity cylinder barrel, a small-capacity pipetting power mechanism for driving the small-capacity piston rod to slide, a large-capacity cylinder barrel communicated with the pipetting gun nozzle, a large-capacity piston rod slidingly connected in the large-capacity cylinder barrel, a large-capacity pipetting power mechanism for driving the large-capacity piston rod to slide, a small-capacity suction head, a large-capacity suction head and a filtering suction head, wherein the pipetting gun nozzle comprises a small-capacity sleeve head part for being matched with the small-capacity suction head and a large-capacity sleeve head part for being matched with the large-capacity suction head or the filtering suction head, a filter screen is arranged in the filtering suction head, and the bottom of the small-capacity suction head is positioned above the filter screen when the small-capacity suction head and the filtering suction head are sleeved on the pipetting gun nozzle. The multifunctional pipetting device further comprises a pipetting integrated seat, wherein the small-capacity cylinder barrel, the small-capacity pipetting mechanism, the large-capacity cylinder barrel and the large-capacity pipetting mechanism are respectively arranged on the pipetting integrated seat, the output end of the small-capacity pipetting mechanism is in driving connection with a small-capacity piston rod, and the output end of the large-capacity pipetting mechanism is in driving connection with a large-capacity piston rod. The multifunctional pipetting device further comprises a transferring mechanism, wherein the pipetting integrated seat is connected with the tail end of the transferring mechanism. The multifunctional pipetting device further comprises a pipette tip bracket, wherein the pipette tip bra