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CN-122007020-A - A high flux sieving mechanism for rice gene function identification

CN122007020ACN 122007020 ACN122007020 ACN 122007020ACN-122007020-A

Abstract

The invention relates to the technical field of agricultural biology, in particular to a high-throughput screening device for identifying rice gene functions, which comprises a bracket and a multi-stage screening bin arranged in the bracket, wherein a vibration screening mechanism, a blowing screening mechanism and a precipitation screening mechanism are sequentially arranged in the multi-stage screening bin along a rice seed feeding path so as to realize step-by-step screening of non-full, empty and mildewed seeds. According to the invention, fine and broken impurities and broken seeds are removed through the reciprocating vibration of the screening net, the directional airflow of the large air cylinder blows, hollow particles and blighted grains with lighter mass are blown away from a main path, then seeds enter the sedimentation tank, under the combined action of continuous water injection and auxiliary airflow of the small air cylinder, the seeds with abnormal density or mildewed interiors float upwards and are carried out through water flow, healthy and full seeds are settled to the bottom of the tank, the whole three-stage screening process is continuously and automatically completed in a single multi-stage screening bin, the flow is smooth, and the selection efficiency is greatly improved.

Inventors

  • YANG PENG

Assignees

  • 广东海洋大学

Dates

Publication Date
20260512
Application Date
20260327

Claims (8)

  1. 1. The utility model provides a high flux sieving mechanism for identification of rice gene function, includes support (1) and installs multistage screening storehouse (2) inside support (1), its characterized in that: the inside of the multistage screening bin (2) is sequentially provided with a vibration screening mechanism, a blowing screening mechanism and a precipitation screening mechanism along a rice seed feeding path so as to realize the gradual screening of the unsaturated, empty, shrunken and mildewed seeds; the vibration screening mechanism is driven to operate by an independent driving mechanism.
  2. 2. The high-throughput screening device for identifying rice gene functions according to claim 1, wherein the vibration screening mechanism comprises a screening net (201) arranged at the top end inside the multistage screening bin (2), the screening net (201) is obliquely arranged in a mode of being low in front and high in back, and a first gap for small particle impurities to fall is formed between the front end of the screening net and the inner wall of the multistage screening bin (2).
  3. 3. The high-throughput screening device for identifying rice gene functions according to claim 1, wherein the driving mechanism comprises a driving motor (4) fixed on the outer wall of the bracket (1), a cam (401) is fixed on an output shaft of the driving motor (4), the top end of the cam (401) is abutted to the bottom surface of the screening net (201), and four corners of the bottom surface of the screening net (201) are connected with the inner wall of the multistage screening bin (2) through spring telescopic rods (402).
  4. 4. The high-throughput screening device for identifying rice gene functions, as set forth in claim 1, wherein the blowing screening mechanism comprises a ventilating duct (202) fixed at the middle part of the front side of the multistage screening bin (2), the upper end and the lower end of the ventilating duct (202) are respectively communicated with a large air duct (203) and a small air duct (208) with different cross-sectional sizes, the air inlet end of the ventilating duct (202) is provided with at least one Roots blower (204), and the air outlets of the large air duct (203) and the small air duct (208) are respectively led to different height positions inside the multistage screening bin (2).
  5. 5. The high-throughput screening device for identifying rice gene functions according to claim 1, wherein a discharge inclined plate (205) is fixed in the multistage screening bin (2), the discharge inclined plate (205) is positioned below the rear end of the screening net (201) and in front of an outlet of the air cylinder (203) and is arranged in a manner of being inclined in a front-high-back low-inclined manner, and gaps are formed between the front end and the rear end of the high-throughput screening device and the inner wall of the multistage screening bin (2).
  6. 6. The high-throughput screening device for identifying rice gene functions, as set forth in claim 1, wherein the sedimentation screening mechanism comprises a sedimentation tank (206) arranged inside the multistage screening bin (2) and close to the bottom, the top surface opening position of the sedimentation tank (206) corresponds to the outlet of the small air duct (208), the bottom surface of the sedimentation tank is provided with a discharge valve (207), a second gap is formed between the rear side wall of the sedimentation tank and the inner wall of the multistage screening bin (2), and a water adding pipeline for injecting water into the sedimentation tank (206) is further arranged on the multistage screening bin (2).
  7. 7. The high-throughput screening device for rice gene function identification of claim 1, wherein the rear side wall of the multistage screening bin (2) is provided with a ventilation net (3) in an embedded manner, wherein the ventilation net corresponds to an outlet area of a large air cylinder (203).
  8. 8. The high-throughput screening device for identifying rice gene functions according to claim 1, wherein a rectangular guard plate (5) is fixed on the outer side of the bottom of the multistage screening bin (2), and a driving motor (4), a cam (401) and a spring telescopic rod (402) are all located outside a space surrounded by the rectangular guard plate (5).

Description

A high flux sieving mechanism for rice gene function identification Technical Field The invention relates to the technical field of agricultural biology, in particular to a high-throughput screening device for rice gene function identification. Background Rice is used as an important grain crop and monocotyledonous mode plant, and research on gene functions of the rice is the basis of genetic improvement. In the research of rice gene function identification, the seed quality of an experimental starting point is crucial, and full, complete and uniform seeds are the basis for ensuring high germination rate, seedling uniformity and subsequent phenotype data accuracy. The traditional gene function identification method relies on manual observation and measurement, and has the problems of low flux, strong subjectivity, low efficiency and the like. Although the existing plant phenotyping techniques have been partially automated, specific culture and high throughput screening for rice at the seedling stage or pre-maturation stage are still significantly inadequate, especially in integrated, low cost, dedicated devices suitable for use in common laboratories. As chinese patent CN104607392a discloses a rice seed screening device, it adopts single-stage vibrating screen structure, only can realize the preliminary separation based on the particle diameter, can't effectively get rid of empty flat grain or inside mildewed seed, and screening precision is limited, and its function is single, and screening flow is dispersed, is difficult to realize the high quality, the efficient pretreatment to the seed before the identification of rice gene function. Therefore, we propose a high throughput screening device for rice gene function identification. Disclosure of Invention The invention aims to solve the problems in the background technology, and provides a high-throughput screening device for identifying rice gene functions. In order to achieve the aim, the high-throughput screening device for identifying the gene functions of the rice comprises a bracket and a multi-stage screening bin arranged in the bracket, wherein a vibration screening mechanism, a blowing screening mechanism and a precipitation screening mechanism are sequentially arranged in the multi-stage screening bin along a rice seed feeding path so as to realize step-by-step screening of non-full, empty and mildewed seeds; the vibration screening mechanism is driven to operate by an independent driving mechanism. In the above-mentioned high flux sieving mechanism for identification of rice gene function, the vibration screening mechanism is including setting up in the screening net on the inside top of multistage screening storehouse, and the screening net is low back high slope and arranges, forms the first clearance that supplies small granule impurity whereabouts between its front end and the inner wall of multistage screening storehouse. In the high-throughput screening device for identifying the functions of rice genes, the driving mechanism comprises a driving motor fixed on the outer wall of the support, a cam is fixed on an output shaft of the driving motor, the top end of the cam is abutted to the bottom surface of the screening net, and four corners of the bottom surface of the screening net are connected with the inner wall of the multistage screening bin through spring telescopic rods. In the high-throughput screening device for identifying rice gene functions, the blowing screening mechanism comprises a ventilating drum fixed at the middle part of the front side of the multistage screening bin, the upper end and the lower end of the ventilating drum are respectively communicated with a large air drum and a small air drum with different cross section sizes, at least one Roots blower is arranged on the air inlet section of the ventilating drum, and the air outlets of the large air drum and the small air drum are respectively led to different height positions inside the multistage screening bin. In the high-throughput screening device for identifying the functions of rice genes, the discharging inclined plates are fixed inside the multi-stage screening bin, are positioned below the rear end of the screening net and in front of the outlet of the large air cylinder, are arranged in a front-high-rear low-inclination mode, and are formed with gaps between the front end and the rear end of the high-throughput screening device and the inner wall of the multi-stage screening bin. In the above-mentioned high flux screening device for identifying rice gene function, the sedimentation screening mechanism includes setting up in the inside sedimentation tank that is close to the bottom of multistage screening storehouse, and the top surface open position of sedimentation tank corresponds with the export of little dryer, and its bottom surface is equipped with the relief valve, is formed with the second clearance between its back lateral wall and the inner wall of