CN-122010092-A - Hydroxylated polyphenol carbon nanomaterial and preparation method and application thereof

CN122010092ACN 122010092 ACN122010092 ACN 122010092ACN-122010092-A

Abstract

The invention discloses a hydroxylated polyphenol carbon nanomaterial, which is prepared by mixing tea polyphenol, a solvent and a hydroxide solution, reacting at 90-100 ℃, and purifying. The method is green and simple in process, the prepared hydroxylated polyphenol carbon nanomaterial has the outstanding advantages of uniform structure, abundant functional groups, strong stability, strong antioxidant activity and the like, can effectively relieve salt stress of cotton, and remodels oxidation-reduction balance in cotton by simulating activities of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD) and the like, so that salt tolerance of cotton is effectively improved. The carbon nanomaterial disclosed by the invention overcomes the defects of uncontrollable activity of a traditional biomass carbon point, high potential risk of a metal nanomaterial and the like, and has a wide application value.

Inventors

WU HONGHONG
LI ZHAOHU
FAN CHENJIE
YANG MINYU
ZHAI SHUWEI
GU JIANGJIANG
MA JIMEI
LIU JIAXI

Assignees

华中农业大学

Dates

Publication Date: 20260512
Application Date: 20251229

Claims (10)

1. The preparation method of the hydroxylated polyphenol carbon nanomaterial is characterized by comprising the following steps of mixing tea polyphenol, a solvent and a hydroxide solution, reacting at 90-100 ℃, and purifying to obtain the hydroxylated polyphenol carbon nanomaterial; the concentration of the hydroxide solution is more than or equal to 0.5g/mL; the mass ratio of the tea polyphenol to the hydroxide is (1-5) (15-35); the particle size of the hydroxylated polyphenol carbon nanomaterial is 2-100 nm.
2. The hydroxylated polyphenol carbon nanomaterial of claim 1, wherein the mass to volume ratio of tea polyphenol to solvent is (1-5) g (30-70) mL.
3. The hydroxylated polyphenol carbon nanomaterial of claim 1, wherein the solvent is selected from at least one of o-dichlorobenzene, toluene, indan.
4. The hydroxylated polyphenol carbon nanomaterial of claim 1, wherein the hydroxide is selected from at least one of sodium hydroxide and potassium hydroxide.
5. The hydroxylated polyphenol carbon nanomaterial of claim 1, wherein the purifying comprises washing with methanol.
6. The application of the hydroxylated polyphenol carbon nanomaterial of any one of claims 1-5 in improving salt stress resistance of plants.
7. The use according to claim 6, wherein increasing salt stress resistance of the plant means increasing at least one of fresh weight, dry weight, stem thickness, true leaf number, plant height of the plant.
8. The application of claim 6, wherein the application concentration of the hydroxylated polyphenol carbon nanomaterial is 0.05-0.55 g/L.
9. The foliar spray nano fertilizer is characterized by comprising the hydroxylated polyphenol carbon nanomaterial of any one of claims 1-8 and a surfactant.
10. The use of the foliar spray nano fertilizer of claim 9 for improving salt stress resistance of plants.

Description

Hydroxylated polyphenol carbon nanomaterial and preparation method and application thereof Technical Field The invention belongs to the technical field of nano materials, and particularly relates to a hydroxylated polyphenol carbon nano material, and a preparation method and application thereof. Background Cotton is an important commercial crop in the world. However, soil salinization has become one of the main abiotic stress factors limiting cotton production, and reducing the toxic effect of salt stress on cotton is a problem to be solved urgently. Salt stress affects cotton growth and development mainly through mechanisms such as ion toxicity, osmotic stress and nutrient imbalance. The high concentration Na + can damage the integrity of cell membranes, interfere the absorption of K +, cause unbalance of cell ion steady state, cause physiological drought due to high osmotic pressure, inhibit seed germination and seedling growth, induce a large amount of active oxygen accumulation due to salt stress, induce oxidative damage, damage chloroplast structure and reduce photosynthesis efficiency. Cotton seedlings are particularly sensitive to salt and are less resistant to salt, which is the critical period in determining cotton yield. The patent CN113396912A discloses a novel application of tea polyphenol in inducing salt resistance of rice seedlings, seed soaking liquid and a salt resistance method, wherein the active ingredient of the rice seed soaking liquid is a tea polyphenol and salicylic acid compound, and the tea polyphenol is a general name of polyphenol compounds rich in tea, and is called TPP for short. The seed soaking liquid can effectively improve the germination rate and the germination vigor of rice seeds after salt stress, and effectively relieve the inhibition effect of the salt stress on the germination of the rice seeds. However, the effect of the seed soaking liquid on salt stress resistance of cotton seedlings is not clear, and the functional material for relieving the salt stress of cotton is deficient currently, so that the salt resistance efficiency and the practicability of the existing material are still to be further improved. Therefore, the development of more materials which have simple preparation process and good salt resistance efficiency and can solve the problem of salt stress of cotton has important significance. Disclosure of Invention In view of the above-mentioned problems of the prior art, it is a primary object of the present invention to provide a hydroxylated polyphenol carbon nanomaterial. The second purpose of the invention is to provide a foliar spray nano fertilizer. The third object of the invention is to provide the application of the hydroxylated polyphenol carbon nanomaterial or foliar spray nano fertilizer in improving salt stress resistance of plants. In order to achieve the above purpose, the present invention is realized by the following technical scheme: The invention discloses a hydroxylated polyphenol carbon nanomaterial, which is prepared by mixing tea polyphenol, a solvent and a hydroxide solution, reacting at 90-100 ℃ and purifying to obtain the hydroxylated polyphenol carbon nanomaterial, wherein the concentration of the hydroxide solution is more than or equal to 0.5g/mL, the mass ratio of the tea polyphenol to the hydroxide is (1-5) (15-35), and the particle size of the hydroxylated polyphenol carbon nanomaterial is 2-100 nm. The invention selects tea polyphenol as a precursor, and aims to prepare the carbon nanomaterial which is simple in process and can relieve salt stress of cotton. The molecular skeleton of tea polyphenol is formed from several aromatic rings, on which the rich hydroxyl groups (OH) are connected, and the invention adopts mild reaction condition, and can retain partial phenolic hydroxyl groups in the tea polyphenol molecule in the carbonization process, and can be based on carbon nano material surface or can be converted into other oxygen-containing functional groups (such as carboxyl and carbonyl). Meanwhile, the hydroxide can be introduced with more polyhydroxy, and finally the hydroxylated polyphenol carbon nanomaterial with more abundant functional groups and stronger antioxidant activity is obtained. The invention further obtains the hydroxylated polyphenol carbon nanomaterial with uniform particle size by controlling the concentration of the hydroxide solution and the reaction temperature. The obtained hydroxylated polyphenol carbon nanomaterial has the advantages of definite structure, stronger hydrogen bond interaction and better water solubility, the nanoscale particle size is favorable for entering plant cells, active oxygen (such as OH, H 2O2) excessively accumulated in cotton under salt stress is directly neutralized, oxidative damage is relieved from the source, cell membranes and chloroplasts are protected, and therefore the salt stress resistance of the cotton is improved more effectively, and the performance is t