CN-122010094-A - Preparation method and application of Chinese herbal medicine-derived carbon dots with pH responsive photodynamic antibacterial and antioxidant activities

Abstract

The invention discloses a preparation method and application of a Chinese herbal medicine source carbon point with pH responsive photodynamic antibacterial and antioxidant activity, comprising the following steps of 1, weighing rheum officinale, soaking in 20% ethanol solution for leaching to obtain rheum officinale leaching solution; step2, after ultrasonic treatment of the rheum officinale leaching solution in the step 1 for 10-15min, adding a proper amount of rheum officinale leaching solution into a ceramic ark, placing the ceramic ark into a muffle furnace, calcining at 260-280 ℃ for 60-90min to obtain black solid, step 3, dissolving the black solid obtained in the step2 into deionized water, ultrasonic treatment for 10-15min, centrifuging to obtain supernatant, and placing the obtained supernatant into a dialysis bag for dialysis to obtain the Chinese herbal medicine source carbon dots. The carbon dots of the Chinese herbal medicine source have pH response and photodynamic response, have good biocompatibility, low toxicity and excellent water solubility, and compared with the raw material rheum officinale, the carbon dots reduce cytotoxicity of rheum officinale and improve the water solubility of effective antibacterial components in the rheum officinale.

Inventors

• XIAO YUXI
• ZHENG HENG
• XU LI

Assignees

• 华中科技大学同济医学院附属同济医院

Dates

Publication Date

20260512

Application Date

20260126

Claims (10)

1. 1. A preparation method of a Chinese herbal medicine-derived carbon dot is characterized by comprising the following steps: Step 1, weighing rheum officinale, soaking the rheum officinale in 20% ethanol solution for leaching to obtain rheum officinale leaching solution; Step 2, after carrying out ultrasonic treatment on the rheum officinale leaching solution in the step 1 for 10-15min, adding a proper amount of rheum officinale leaching solution into a ceramic ark, placing the ceramic ark in a muffle furnace, and calcining at 260-280 ℃ for 60-90min to obtain black solid; and 3, dissolving the black solid obtained in the step 2 in deionized water, performing ultrasonic treatment for 10-15min, centrifuging to obtain supernatant, and placing the obtained supernatant into a dialysis bag for dialysis to obtain the Chinese herbal medicine source carbon point.
2. 2. The preparation method of claim 1, wherein in the step 1, the percentage of the rheum officinale and ethanol solution is 10g (100-150) mL.
3. 3. The method according to claim 1, wherein in step 3, the dialysis bag has a molecular weight cut-off of 10000Da.
4. 4. A Chinese herbal medicine-derived carbon dot is characterized in that the carbon dot is prepared by the preparation method of any one of claims 1-3.
5. 5. The use of the carbon dots of herbal origin of claim 4 for antibacterial purposes.
6. 6. The method of claim 5, wherein the antibacterial agent is an antibacterial agent against Staphylococcus aureus.
7. 7. The use of carbon dots derived from Chinese herbal medicine as claimed in claim 4 in antioxidation.
8. 8. The use of carbon dots derived from Chinese herbal medicine as claimed in claim 4 in detection of heavy metal ions and biological small molecules.
9. 9. The use of the carbon dot derived from Chinese herbal medicine of claim 4 in intracellular microenvironment monitoring.
10. 10. The use of the carbon dot of herbal origin of claim 4 in the field of fluorescence imaging.

Description

Preparation method and application of Chinese herbal medicine-derived carbon dots with pH responsive photodynamic antibacterial and antioxidant activities Technical Field The invention belongs to the technical field of biomedical nano materials, and particularly relates to a preparation method and application of a Chinese herbal medicine-derived carbon point with pH responsive photodynamic antibacterial and antioxidant activities. Background Bacterial infections are a major risk to human health, and the primary means of coping with bacterial infections is currently the use of antibiotics. However, abuse of traditional small molecule antibiotics, bacterial evolution, etc. can lead to the generation of a large number of drug-resistant bacteria, which pose a serious threat to human life health, especially cross-resistant bacteria. Meanwhile, the traditional nano antibacterial material has the problems of complex process, harsh reaction conditions (such as high temperature and high pressure and noble metal catalysis) in part of the preparation method, high production cost, low solubility, unstable active ingredients and the like. This results in the inability of the active antimicrobial components of the herbal medicine to exist in significant amounts in water in an effective stable form, and in the inability to precisely target the site of infection, control the release rate. Therefore, development of a novel drug having a highly potent antibacterial activity is urgent. In recent years, various carbon-based nanomaterials, such as carbon dots, have become a novel nano-antibacterial agent having very promising application prospect due to their unique physicochemical properties, such as nanoscale size, high stability, good water solubility and biocompatibility, etc. Disclosure of Invention The invention aims to provide a preparation method and application of a Chinese herbal medicine source carbon dot with pH responsive photodynamic antibacterial and antioxidant activities, wherein the Chinese herbal medicine source carbon dot has uniform particle size distribution (2 nm), good water solubility, low cytotoxicity (cell survival rate is more than or equal to 95%, and concentration is 200 mug/mL) and good biocompatibility, and provides a guarantee for application in the biomedical field. In order to achieve the above purpose, the present application adopts the following technical scheme: in a first aspect, the invention provides a method for preparing carbon dots from Chinese herbal medicine, comprising the following steps: Step 1, weighing rheum officinale, soaking the rheum officinale in 20% ethanol solution for leaching to obtain rheum officinale leaching solution; Step 2, after ultrasonic treatment of the rheum officinale leaching solution in the step 1 for 10-15min, adding a proper amount of rheum officinale leaching solution into a ceramic ark, and calcining at 260-280 ℃ for 60-90min to obtain black solid; And 3, dissolving the black solid obtained in the step 2 in deionized water, performing ultrasonic treatment for 10-15min, centrifuging to obtain supernatant, and placing the obtained supernatant into a dialysis bag for dialysis to obtain the Chinese herbal medicine source carbon dot. In the technical scheme, in the step 1, the percentage of the rheum officinale and ethanol solution is 10g (100-150) mL. In the above technical scheme, in step 3, the cut-off molecular weight of the dialysis bag is 10000Da. In a second aspect, the invention provides a carbon dot derived from Chinese herbal medicine, which is prepared by the preparation method. In a third aspect, the invention provides an application of the carbon dots derived from the Chinese herbal medicine in the aspect of antibiosis. In the above technical scheme, the antibacterial effect is against staphylococcus aureus. In a fourth aspect, the invention provides an application of the carbon dots derived from the Chinese herbal medicine in antioxidation. In a fifth aspect, the invention provides an application of the carbon dots derived from the Chinese herbal medicine in detection of heavy metal ions and biological small molecules. In a sixth aspect, the present invention provides an application of the above-mentioned carbon dots derived from Chinese herbal medicine in intracellular microenvironment monitoring. In a seventh aspect, the present invention provides an application of the above-mentioned carbon dots derived from Chinese herbal medicine in the field of fluorescence imaging. The invention has the beneficial effects that: 1. The preparation method is simple, adopts a muffle furnace calcination method, does not need complex equipment and subsequent modification steps, has mild reaction conditions and low cost, and is easy for large-scale production. 2. The carbon dots of the Chinese herbal medicine source have pH response and photodynamic response, can exert different capacities according to actual requirements, and are suitable for application in