CN-122010095-A - Nitrogen-doped biomass carbon dot, method for promoting plant root growth and application thereof

Abstract

The invention relates to a method for promoting plant root growth by doping biomass carbon dots with nitrogen and application thereof, belonging to the technical field of agricultural nano materials. In the application of the nitrogen-doped biomass carbon dot, the nitrogen-doped biomass carbon dot can promote root growth of various types of plants such as grain crops, vegetables, model plants and ornamental plants in a broad spectrum under normal conditions or salt stress conditions. According to the preparation method of the nitrogen-doped biomass carbon dot, agricultural waste is used as a carbon source, urea is used as a nitrogen source, the nitrogen-doped biomass carbon dot with the surface rich in carboxyl and amino is specifically prepared through a hydrothermal process, the prepared nitrogen-doped biomass carbon dot is small in size, the surface rich in carboxyl and amino, good in biocompatibility and low in toxicity, the yield is up to 22.3%, the cost is low, and the nitrogen-doped biomass carbon dot is green and sustainable and can be used for large-scale production.

Inventors

• MA RUONAN
• ZHU YUPAN
• JIAO CHENG
• WANG YUE
• HUANG KANGHUA
• Pan Nengquan

Assignees

• 郑州大学
• 合浦华泉农业发展有限公司

Dates

Publication Date

20260512

Application Date

20260210

Claims (10)

1. 1. The application of the nitrogen-doped biomass carbon dots is characterized in that the application of the nitrogen-doped biomass carbon dots to promote plant root growth under normal conditions or salt stress conditions is carried out, and the concentration of the nitrogen-doped biomass carbon dots is 0.1-100 mg/L.
2. 2. The application of the nitrogen-doped biomass carbon dot according to claim 1, wherein the salt stress is salt stress caused by sodium chloride, and the concentration of the sodium chloride is 100-150 mM.
3. 3. The application of the nitrogen-doped biomass carbon dots according to claim 1, wherein the average particle size of the nitrogen-doped biomass carbon dots is 1.4-4.2 nm.
4. 4. The application of the nitrogen-doped biomass carbon dot according to claim 1, wherein the nitrogen-doped biomass carbon dot is prepared by taking corn straw as a carbon source and urea as a nitrogen source through a hydrothermal reaction, the temperature of the hydrothermal reaction is 180-220 ℃, the time of the hydrothermal reaction is 6-10 hours, and the mass ratio of the corn straw to the urea is 1 (0.6-3).
5. 5. The use of nitrogen-doped biomass carbon dots according to claim 1, wherein the plant is one or more of a food crop, a vegetable, a model plant, an ornamental plant.
6. 6. The use of nitrogen-doped biomass carbon dots according to claim 1, wherein the application mode of the nitrogen-doped biomass carbon dots comprises one or more of seed soaking, root watering, foliar spraying.
7. 7. A method for promoting plant root growth is characterized by comprising the following steps of seed soaking and/or root irrigation and/or leaf surface spraying of plants by adopting nitrogen-doped biomass carbon dot solution with the concentration of 0.1-100 mg/L.
8. 8. The preparation method of the nitrogen-doped biomass carbon dot is characterized by comprising the steps of pre-treating corn stalks, pickling, dispersing the pickled corn stalks and urea in water, performing hydrothermal reaction, and purifying to obtain the nitrogen-doped biomass carbon dot, wherein the nitrogen-doped biomass carbon dot promotes plant root growth under normal conditions or salt stress conditions.
9. 9. The nitrogen-doped biomass carbon dot according to claim 8, wherein the pretreatment is to crush and screen corn stalks to 80-100 meshes, and the acid washing is to soak the corn stalks for 12-24 hours with 0.05-0.1 mol/L HCl solution.
10. 10. The nitrogen-doped biomass carbon dot according to claim 8, wherein the mass volume ratio of the corn stalks to the water is 1g (10-30) mL, the mass ratio of the corn stalks to the urea is 1 (0.6-3), the temperature of the hydrothermal reaction is 180-220 ℃, and the time of the hydrothermal reaction is 6-10 h.

Description

Nitrogen-doped biomass carbon dot, method for promoting plant root growth and application thereof Technical Field The invention belongs to the technical field of agricultural nano materials, and particularly relates to a method for doping biomass carbon dots with nitrogen and promoting plant root growth and application thereof. Background Along with the shortage of cultivated land resources and the expansion of salinization area, the development of efficient and safe modern agricultural technology has become a key strategy for guaranteeing the grain safety. The traditional plant growth regulator in the current agricultural production has very wide application, such as Naphthalene Acetic Acid (NAA) can effectively promote the growth of crops, but is easy to degrade under the action of light and microorganisms, so that the action period is short, the application is frequent, the labor and economic cost are increased, chemical residues can be caused, and hidden danger is brought to soil microecology and agricultural product safety. In addition, under bad growth environments such as salt stress, the promotion effect of the traditional plant growth regulator on plant root growth is often obviously limited, and the regulation and control effect is difficult to continuously play. In this context, agricultural nanomaterial technology has evolved. Carbon Dots (CDs) are taken as novel Carbon-based nano materials, and have the characteristics of small particle size, good water solubility, high biocompatibility, adjustable surface function and the like, so that the novel Carbon-based nano materials have wide application prospects in the aspects of plant growth regulation and stress resistance improvement, and become current research hot spots. The traditional preparation method of the carbon dots for promoting plant growth mostly adopts small molecular chemical raw materials such as citric acid, glucose, ethylenediamine, polyethyleneimine and the like, and adopts a hydrothermal method, a microwave method or a solvothermal method. The carbon dots prepared by the traditional preparation method have certain controllability in structure and performance, but the preparation process depends on chemical precursors, the raw material cost is relatively high, and potential environmental residues and ecological safety problems still need to be concerned in agricultural application. The invention patent with the application publication number of CN114190188A discloses a method for relieving plant growth resistance under high-concentration rare earth element stress and application, and the preparation method of the carbon quantum dots in the invention adopts citric acid and urea as raw materials, and adopts a microwave method for synthesis, so that the cost of the raw materials is relatively high. Therefore, development of a biomass carbon dot material which is green in raw materials, simple and convenient in preparation process and capable of promoting plant root growth under normal growth conditions and salt stress conditions is needed, and a new technical means is provided for plant growth vigor, environment adaptability and saline-alkali utilization. Disclosure of Invention The first object of the invention is to provide a nitrogen-doped biomass carbon dot which has small size, good biocompatibility and water solubility. A second object of the invention is to provide a use of nitrogen doped biomass carbon dots. It is a third object of the present invention to provide a method for promoting plant root growth. In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: In the first aspect, the invention provides application of nitrogen-doped biomass carbon dots, wherein the application of the nitrogen-doped biomass carbon dots in promoting plant root growth under normal conditions or salt stress conditions is provided, and the concentration of the nitrogen-doped biomass carbon dots is 0.1-100 mg/L. Preferably, the salt stress is salt stress caused by sodium chloride, and the concentration of the sodium chloride is 100-150 mM. Preferably, the average particle size of the nitrogen-doped biomass carbon dots is 1.4-4.2 nm. The nitrogen-doped biomass carbon dot is prepared by taking corn straw as a carbon source and urea as a nitrogen source through a hydrothermal reaction, wherein the temperature of the hydrothermal reaction is 180-220 ℃, the time of the hydrothermal reaction is 6-10 h, and the mass ratio of the corn straw to the urea is 1 (0.6-3). Preferably, the plant is one or more of a food crop, a vegetable, a model plant, an ornamental plant. Preferably, the application mode of the nitrogen-doped biomass carbon dots comprises one or more of seed soaking, root irrigation and leaf surface spraying. In a second aspect, the invention provides a method for promoting plant root growth, which comprises the following steps of seed soaking and/or root watering and/or leaf surface spraying of plant