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CN-122010610-A - Targeted biological bacteria coupling method for comprehensive treatment of salt damage, heavy metal and pesticide residues of coastal vegetables and application thereof

CN122010610ACN 122010610 ACN122010610 ACN 122010610ACN-122010610-A

Abstract

The invention discloses a targeted biological bacterium coupling method and application for comprehensive treatment of salt damage, heavy metals and pesticide residues of coastal vegetables, which relate to the field of agricultural biotechnology and soil remediation, the method is characterized in that bacillus subtilis, bacillus mucilaginosus, bacillus laterosporus, pseudomonas fluorescens and arbuscular mycorrhizal fungi are subjected to coupling symbiotic culture with host original flora extracted from rhizosphere soil of healthy vegetables, so that target flora with multiple functions is formed. The functional flora is compounded with a nutrition carrier containing humic acid potassium fulvate, seaweed extract and specific salt-resistant auxiliary agent to prepare the multifunctional water-soluble fertilizer or microbial inoculum. After the composite barrier agent is applied to coastal vegetable production bases, the synergistic effects of degrading soil pesticide residues, passivating heavy metals, enhancing vegetable salt tolerance and improving soil fertility and microorganism diversity can be synchronously realized, the problem of composite barrier in coastal vegetable production is fundamentally solved, and the vegetable yield, quality and safety are ensured.

Inventors

  • ZHANG BIN

Assignees

  • 张彬

Dates

Publication Date
20260512
Application Date
20251222

Claims (10)

  1. 1. The targeted biological bacteria coupling method for comprehensive treatment of salt damage, heavy metals and pesticide residues of the coastal vegetables is characterized by comprising the following steps: a. constructing a target biological strain resource library, and screening a functional single strain; b. purifying and compounding the single strain; c. Coupling symbiotic culture is carried out on the compound flora and host original flora extracted from healthy vegetable rhizosphere soil; d. The compound functional flora after coupling culture is mixed with a nutrition carrier containing an anti-salt auxiliary agent to prepare a microbial agent or a water-soluble fertilizer for vegetable cultivation.
  2. 2. The method for coupling targeted biological bacteria for comprehensive treatment of salt damage, heavy metals and pesticide residues of coastal vegetables according to claim 1, wherein the functional single strain in the step a comprises at least three of bacillus subtilis, bacillus mucilaginosus, bacillus laterosporus, pseudomonas fluorescens and arbuscular mycorrhizal fungi.
  3. 3. The method for coupling targeted biological bacteria for comprehensive treatment of salt damage, heavy metals and pesticide residues of coastal vegetables according to claim 2, wherein the concentration requirements of each strain in functional biological bacteria are that bacillus subtilis is more than or equal to 5×10 8 CFU/ml, bacillus mucilaginosus is more than or equal to 3×10 8 CFU/ml, brevibacillus laterosporus is more than or equal to 2×10 8 CFU/ml, pseudomonas fluorescens is more than or equal to 1×10 8 CFU/ml, arbuscular mycorrhizal fungi is more than or equal to 50 spores/gram, and host original bacteria is more than or equal to 1×10 8 CFU/ml.
  4. 4. The method for coupling targeted biological bacteria for comprehensive treatment of salt damage, heavy metals and pesticide residues of coastal vegetables according to claim 1, wherein the nutritional carrier in the step d comprises potassium humate, seaweed extract, amino acid chelated trace elements, potassium dihydrogen phosphate, sucrose or molasses, and salt-resistant auxiliary agents.
  5. 5. The targeted biological bacteria coupling method for comprehensive treatment of salt damage, heavy metals and pesticide residues of coastal vegetables is characterized by comprising, by weight, 1-1.5 parts of urea, 0.003-0.006 part of naphthylacetic acid, 0.02-0.06 part of humic acid, 0.3-0.6 part of mannitol, 0.1-0.4 part of ammonium sulfate, 0.01-0.06 part of proline, 0.01-0.04 part of arginine, 0.005-0.01 part of surfactant and 90-100 parts of water.
  6. 6. The targeted biological bacteria coupling method for comprehensive treatment of salt damage, heavy metals and pesticide residues of coastal vegetables according to claim 5, wherein the composition of the nutritional carrier comprises 40-60kg of humic acid potassium fulvate, 20-40kg of seaweed extract, 5-15kg of amino acid chelated trace elements, 15-25kg of potassium dihydrogen phosphate, 10-20kg of sucrose or molasses and 30-50kg of salt resistance auxiliary agent in each ton of finished water-soluble fertilizer.
  7. 7. A multifunctional targeted biological bacteria water-soluble fertilizer or microbial inoculum prepared by the method of any one of claims 1-6.
  8. 8. The cultivation method for improving the yield, quality and safety of the coastal vegetables is based on the multifunctional targeted biological fungus water-soluble fertilizer or the microbial inoculum according to claim 7, and is characterized in that the multifunctional targeted biological fungus water-soluble fertilizer or the microbial inoculum is applied periodically in the growth and development critical period of the vegetables.
  9. 9. The cultivation method according to claim 8, wherein said vegetable is a leafy vegetable or a fruit vegetable; The key growth and development period comprises a seedling period, a vigorous growth period and a pre-harvest period; The application mode is that root irrigation or drip irrigation is carried out after 500-800 times of dilution, and the salt-resistant auxiliary agent is sprayed on leaf surfaces in a period of obvious salt stress.
  10. 10. Use of the method according to any one of claims 1-6 or the multifunctional targeted biological bacterial water-soluble fertilizer or microbial inoculum according to claim 7 for repairing coastal salinized, heavy metal contaminated and pesticide residue soil.

Description

Targeted biological bacteria coupling method for comprehensive treatment of salt damage, heavy metal and pesticide residues of coastal vegetables and application thereof Technical Field The invention relates to the technical field of agricultural biotechnology and soil remediation, in particular to a construction method of a targeted microbial flora and application thereof in comprehensively treating problems of soil salt damage, heavy metal pollution and pesticide residue of coastal vegetable production bases. Background The coastal areas of China have a large number of vegetable production bases, but the long-term intensive production of the bases generally faces the problem of combined pollution caused by factors such as seawater backflow, chemical fertilizer and pesticide abuse, wherein the problems are that vegetables are physiological drought, nutrient absorption is unbalanced and growth is hindered due to salinization of soil, heavy metals such as cadmium, lead and arsenic in the soil are easy to be enriched by the vegetables and endanger human health through a food chain, and the continuous cropping obstacle and soil-borne disease frequently cause pesticide dependence to pollute the soil and agricultural products. The existing treatment technology is mostly 'single-shot', such as degrading pesticide residues by using a single microbial inoculum, treating heavy metals by using a passivating agent, or relieving salt damage by using a salt-resistant agent, however, the salt damage can only be relieved by using a single salt-resistant agent, the problems of heavy metals and pesticide residues cannot be solved, the effect of the heavy metal passivating agent (such as lime and bentonite) is short, leaching and invalidation are easy to occur along with irrigation, the microbial inoculum is mostly a single strain, the function is limited, only a certain pollution type can be aimed at, the compatibility of the strain and a soil primordial flora is poor, the activity is easy to be inhibited by salt stress, the nutrition carrier and biological bacteria are unreasonable, the requirement of flora growth and vegetable nutrition supply are difficult to meet, the comprehensive treatment effect is poor, the vegetable yield, the quality and the safety cannot be considered, and therefore, the green, efficient and sustainable technology capable of simultaneously targeting the salt damage, the heavy metals and the pesticide residues is developed to be an urgent demand for sustainable development of coastal agriculture. Disclosure of Invention The invention aims to provide a targeted biological bacterium coupling method and application capable of synergistically treating salt damage, heavy metals and pesticide residues of a coastal vegetable base. The technical scheme for achieving the purpose is that the embodiment of the invention provides a targeted biological bacteria coupling method for comprehensive treatment of salt damage, heavy metals and pesticide residues of coastal vegetables, which comprises the following steps: a. constructing a target biological strain resource library, and screening a functional single strain; b. purifying and compounding the single strain; c. Coupling symbiotic culture is carried out on the compound flora and host original flora extracted from healthy vegetable rhizosphere soil; d. The compound functional flora after coupling culture is mixed with a nutrition carrier containing an anti-salt auxiliary agent to prepare a microbial agent or a water-soluble fertilizer for vegetable cultivation. The target biological bacteria coupling method provided by the embodiment of the invention combines the soil primordial host flora to carry out coupling symbiotic culture by screening and compounding the multifunctional single bacterial strain, improves the compatibility and environmental adaptability of the flora, combines the composite functional flora with the nutritional carrier containing the salt-resistant auxiliary agent, can play roles of degrading pesticide residues, passivating heavy metals and improving soil structures of the flora, can also relieve salt damage through the salt-resistant auxiliary agent, supplement nutrition required by vegetable growth, realize the synergistic treatment of salt damage, heavy metals and pesticide residues, and simultaneously improve the stress resistance and growth performance of the vegetables. Optionally, the functional single strain in step a comprises at least three of bacillus subtilis, bacillus mucilaginosus, bacillus laterosporus, pseudomonas fluorescens and arbuscular mycorrhizal fungi. Five bacterial strain combinations with specific functions are selected, wherein bacillus subtilis can degrade organophosphorus pesticide residues and inhibit pathogenic bacteria, bacillus mucilaginosus can activate soil nutrients and deactivate heavy metals, bacillus laterosporus can degrade pyrethroid pesticide residues and strengthen stress resistance of vegetable