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CN-122010618-A - Silicon ecological enzyme agricultural preparation with biological activation and physical protection functions and preparation method thereof

CN122010618ACN 122010618 ACN122010618 ACN 122010618ACN-122010618-A

Abstract

The invention relates to the technical field of biological agriculture, and discloses a silicon ecological enzyme agricultural preparation with biological activation and physical protection functions and a preparation method thereof. The invention discloses a preparation method of a silicon ecological enzyme agricultural preparation with double functions of biological activation and physical protection, which comprises the following steps of 1) adding a fermentation supplement into organic waste to obtain a fermentation raw material, then adding a compound microbial agent into the fermentation raw material to perform sectional fermentation to obtain fermentation liquor, 2) adding the compound enzyme preparation into the fermentation liquor obtained in the step 1), performing enzymolysis to obtain enzymolysis liquor, and 3) supplementing a silicon source into the enzymolysis liquor obtained in the step 2) to obtain the silicon ecological enzyme agricultural preparation with double functions of biological activation and physical protection. The silicon ecological enzyme agricultural preparation with biological activation and physical protection functions has good soil environment improvement effect and good crop performance improvement effect.

Inventors

  • YANG FAN
  • YU NA

Assignees

  • 泉和川生态农业发展(山东)有限公司

Dates

Publication Date
20260512
Application Date
20260204

Claims (10)

  1. 1. The preparation method of the silicon ecological enzyme agricultural preparation with biological activation and physical protection functions is characterized by comprising the following steps: 1) Adding the fermentation supplement into organic waste to obtain a fermentation raw material, and then adding a compound microbial agent into the fermentation raw material to perform sectional fermentation to obtain fermentation liquor; 2) Adding the compound enzyme preparation into the fermentation broth obtained in the step 1), and carrying out enzymolysis to obtain an enzymolysis broth; 3) And (3) supplementing a silicon source into the enzymolysis liquid obtained in the step (2) to obtain the silicon ecological enzyme agricultural preparation with biological activation and physical protection functions.
  2. 2. The method for preparing the silicon ecological enzyme agricultural preparation with the biological activation and physical protection functions according to claim 1, wherein in the step 1), The fermentation supplement is prepared by mixing humic acid, seaweed extract, bentonite and chitin according to the mass ratio of (4-6) to (2-4) to (0.5-1.5); the preparation method of the organic waste comprises the following steps of mixing agricultural waste and livestock manure, regulating the C/N ratio to 30:1 to obtain a premix, and then carrying out physical-chemical synergistic treatment on the premix to obtain the organic waste.
  3. 3. The method for preparing the silicon ecological enzyme agricultural preparation with the biological activation and physical protection functions according to claim 1, wherein in the step 1), the mass ratio of the fermentation supplement to the organic waste is 1:30-40.
  4. 4. The preparation method of the silicon ecological enzyme agricultural preparation with the biological activation and physical protection functions, which is disclosed in claim 1, is characterized in that in the step 1), the composite microbial agent is prepared by mixing bacillus subtilis, methanosarcina thermophila, bacillus megatherium and azotobacter chroococcus in the mass ratio of (5-7) to (3-5) to (2-4) to (1-3).
  5. 5. The preparation method of the silicon ecological enzyme agricultural preparation with biological activation and physical protection functions, which is characterized in that in the step 1), the mass ratio of the fermentation raw material to the composite microbial agent is 50:1-3.
  6. 6. The method for preparing the silicon ecological enzyme agricultural preparation with biological activation and physical protection functions according to claim 1, wherein in the step 1), the sectional fermentation is specifically: In the aerobic starting stage, in an environment with the oxygen content of 20%, controlling the stirring rotating speed to be 60r/min, and fermenting for 12-36 h at a constant temperature of 38-42 ℃; The anaerobic fermentation stage comprises the steps of fermenting for 4-6 days at a constant temperature of 45-48 ℃ in an environment with the oxygen content of less than or equal to 1%, wherein the stirring rotating speed is controlled to be 50r/min every 12 hours, and stirring for 15min; and in the microaerobic after-ripening stage, fermenting for 5-7 days at a constant temperature of 35-37 ℃ in an environment with the oxygen content of 5%, wherein the stirring rotating speed is controlled to be 30r/min every 24 hours, and stirring is carried out for 5min.
  7. 7. The preparation method of the silicon ecological enzyme agricultural preparation with the biological activation and physical protection functions, which is disclosed in claim 1, is characterized in that in the step 2), the compound enzyme preparation is prepared by mixing cellulase, protease, hemicellulase and pectinase according to the mass ratio of (2-4) to (1-3) to (0.5-1.5).
  8. 8. The method for preparing the silicon ecological enzyme agricultural preparation with the biological activation and physical protection functions, which is characterized in that in the step 2), the feed liquid ratio of the complex enzyme preparation to the fermentation liquor is 1g to (20-30) mL.
  9. 9. The method for preparing a silicon ecological enzyme agricultural preparation with biological activation and physical protection functions according to claim 1, wherein in the step 3), the supplementary silicon source specifically comprises the following steps: 1) Adding soluble silicon into the enzymolysis liquid, uniformly mixing, and then adding EDTA-2Na with the weight of 0.3 percent for reaction to obtain the soluble silicon-enzymolysis liquid; 2) Adding slow-release silicon into the soluble silicon-enzymolysis liquid obtained in the step 1), uniformly mixing, adding 0.1% genipin after ultrasonic treatment, and crosslinking to obtain the silicon ecological enzyme agricultural preparation.
  10. 10. A silicon ecological enzyme agricultural preparation with dual functions of biological activation and physical protection prepared by the preparation method of the silicon ecological enzyme agricultural preparation with dual functions of biological activation and physical protection as claimed in any one of claims 1 to 9.

Description

Silicon ecological enzyme agricultural preparation with biological activation and physical protection functions and preparation method thereof Technical Field The invention relates to the technical field of biological agriculture, in particular to a silicon ecological enzyme agricultural preparation with biological activation and physical protection functions and a preparation method thereof. Background With the improvement of the sustainable development requirement of modern agriculture, the development of biological agricultural preparations by utilizing organic wastes has become an important direction for recycling resources. The traditional organic fertilizer or microbial fertilizer mainly improves soil fertility by supplementing organic matters and beneficial bacteria, has single function and has limited effect in exciting the potential of crops. Silicon has proven to be a desirable element for many crops, and the application of silicon can enhance the cell wall strength of the crops and form a physical barrier, thereby improving the resistance of the crops to diseases, insect pests and stress. However, single silicon fertilizer products often lack biological activity and do not adequately improve soil and stimulate crop growth. At present, there is also an attempt to simply mix organic matters with silicon fertilizer, but due to the property difference of the organic matters and the silicon fertilizer, a stable and synergistic system is difficult to form, and the effect is poor. Therefore, there is an urgent need in the art for a novel agricultural preparation that can organically combine the biological activity of organic waste with the physical protection function of silicon element to achieve synergistic effect. Disclosure of Invention The invention aims to provide a silicon ecological enzyme agricultural preparation with biological activation and physical protection functions and a preparation method thereof. In order to achieve the above purpose, the present invention provides the following technical solutions: One of the technical schemes of the invention is as follows: a preparation method of a silicon ecological enzyme agricultural preparation with biological activation and physical protection functions comprises the following steps: 1) Adding the fermentation supplement into organic waste to obtain a fermentation raw material, and then adding a compound microbial agent into the fermentation raw material to perform sectional fermentation to obtain fermentation liquor; 2) Adding the compound enzyme preparation into the fermentation broth obtained in the step 1), and carrying out enzymolysis to obtain an enzymolysis broth; 3) And (3) supplementing a silicon source into the enzymolysis liquid obtained in the step (2) to obtain the silicon ecological enzyme agricultural preparation with biological activation and physical protection functions. Further, in the step 1), the fermentation supplement is formed by mixing humic acid, seaweed extract, bentonite and chitin according to the mass ratio of (4-6) to (2-4) to (0.5-1.5). Further, in the step 1), the preparation method of the organic waste comprises the steps of mixing agricultural waste and livestock manure, adjusting the C/N ratio to 30:1 to obtain a premix, and then carrying out physical-chemical synergistic treatment on the premix to obtain the organic waste. Further, the physical-chemical cooperative treatment is specifically that ultrasonic frequency is controlled to be 40kHz, power is controlled to be 300W, the premix is subjected to ultrasonic treatment for 15-25 min, and then 0.5 wt% of rhamnolipid is added and uniformly mixed. Further, in the step 1), the mass ratio of the fermentation supplement to the organic waste is 1:30-40. Further, in step 1), after the fermentation supplement is added to the organic waste, the pH is adjusted to 6.5 by using a pH adjuster, and the water content is adjusted to 60% by using water. Further, in the step 1), the compound microbial agent is formed by mixing bacillus subtilis, methanosarcina thermophila, bacillus megatherium and azotobacter chroococcus in a mass ratio of (5-7) to (3-5) to (2-4) to (1-3). Further, in the step 1), the mass ratio of the fermentation raw material to the composite microbial agent is 50:1-3. Further, in step 1), the step of fermenting includes: In the aerobic starting stage, in an environment with the oxygen content of 20%, controlling the stirring rotating speed to be 60r/min, and fermenting for 12-36 h at a constant temperature of 38-42 ℃; The anaerobic fermentation stage comprises the steps of fermenting for 4-6 days at a constant temperature of 45-48 ℃ in an environment with the oxygen content of less than or equal to 1%, wherein the stirring rotating speed is controlled to be 50r/min every 12 hours, and stirring for 15min; and in the microaerobic after-ripening stage, fermenting for 5-7 days at a constant temperature of 35-37 ℃ in an environment with the oxygen content of