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CN-122010621-A - Multifunctional biological source liquid medical fertilizer and preparation method thereof

CN122010621ACN 122010621 ACN122010621 ACN 122010621ACN-122010621-A

Abstract

The invention belongs to the technical field of fertilizers, and particularly relates to a multifunctional biological source liquid medical fertilizer and a preparation method thereof, wherein the preparation method of the multifunctional biological source liquid medical fertilizer comprises the following steps of S1, mixing and stirring shrimp shell powder and dilute nitric acid, carrying out solid-liquid separation, S2, carrying out enzymolysis on decalcified and defatted shrimp shell powder by adopting compound protease, carrying out solid-liquid separation, S3, carrying out mixed reaction of chitin residues and potassium hydroxide, carrying out solid-liquid separation, S4, carrying out enzymolysis on chitosan residues by adopting chitosan hydrolase, S5, mixing brown coal dust and potassium hydroxide residual liquid to prepare potassium fulvate pure liquid, S6, mixing amino acid liquid, chitosan oligosaccharide liquid and potassium fulvate pure liquid, adding avermectin microcapsules to obtain the multifunctional biological source liquid medical fertilizer, and carrying out multi-component compounding to enable nutrition of the medical fertilizer to be more comprehensive and promote fruits.

Inventors

  • WEN YAXIONG
  • TAN SHIYONG
  • ZHOU YAN
  • WU XIANG

Assignees

  • 湖南神隆科技有限公司

Dates

Publication Date
20260512
Application Date
20260206

Claims (9)

  1. 1. The preparation method of the multifunctional biological source liquid medical fertilizer is characterized by comprising the following steps: S1, mixing and stirring shrimp shell powder and dilute nitric acid, carrying out solid-liquid separation to obtain a first precipitate and a first liquid, washing the first precipitate by deionized water, mixing and soaking the washed first precipitate and ethanol, drying to obtain decalcified and defatted shrimp shell powder, and recovering the first liquid and washing water and mixing to obtain a residual nitric acid solution; S2, carrying out enzymolysis on the decalcified and defatted shrimp shell powder by adopting compound protease, and carrying out solid-liquid separation to obtain amino acid liquid and chitin residues; s3, mixing the chitin residue with potassium hydroxide for reaction, carrying out solid-liquid separation to obtain a second precipitate and a second liquid, washing the second precipitate by adopting deionized water, drying the second precipitate to obtain chitosan residue, recovering the second liquid and washing water, and mixing to obtain potassium hydroxide residual liquid; S4, carrying out enzymolysis on the chitosan residues by adopting chitosan hydrolase, and filtering by an ultrafiltration membrane to obtain chitosan oligosaccharide liquid; S5, crushing lignite, sieving and drying to obtain brown coal dust, mixing and stirring the brown coal dust and the potassium hydroxide residual liquid, performing solid-liquid separation to obtain a potassium fulvate crude liquid, adding the nitric acid residual liquid into the brown coal dust, stirring and filtering to obtain potassium humate precipitate and third liquid, and regulating the pH value of the third liquid to 7.5-8.0 by adopting the potassium hydroxide residual liquid to obtain a potassium fulvate pure liquid; S6, mixing the amino acid liquid, the chitosan oligosaccharide liquid and the potassium fulvate pure liquid according to a preset proportion, adding a buffering agent, uniformly stirring to obtain a premix, adding an avermectin microcapsule and a surfactant, and stirring and filtering to obtain the multifunctional biological source liquid medical fertilizer.
  2. 2. The preparation method of the multifunctional biological source liquid medical fertilizer according to claim 1 is characterized in that in the step S1, the shrimp shell powder and 0.8-1.2 mol/L nitric acid are mixed according to a solid-to-liquid ratio of 1g (12-15) mL and stirred for 1-1.5 h, the first precipitate and the first liquid are obtained after solid-liquid separation, deionized water is adopted to wash the first precipitate until the pH of washing water is 6-6.5, then the washed first precipitate and 95wt% ethanol are mixed according to a solid-to-liquid ratio of 1g (8-10) mL and soaked for 1-1.5 h, the decalcified defatted shrimp shell powder is obtained after drying, and the first liquid and the washing water are recovered and mixed to obtain the nitric acid residual liquid.
  3. 3. The preparation method of the multifunctional biological source liquid medical fertilizer according to claim 1 is characterized in that the step S2 is specifically characterized in that decalcified and defatted shrimp shell powder is mixed with deionized water according to a solid-to-liquid ratio of 1g (20-25), the pH value of the mixture is regulated to 7.5-8.2, then composite protease which is 0.7% -1.2% of the mass of decalcified and defatted shrimp shell powder is added, enzymolysis is carried out for 7-9 h under the conditions of 53-60 ℃ and a stirring speed of 200-220 rpm, the temperature is raised to 85-90 ℃ and the temperature is kept for 12-15 min for inactivating enzyme, supernatant and chitin residues are obtained through centrifugal separation at a rotating speed of 3000-3500 rpm, the supernatant is concentrated to obtain amino acid liquid, and the concentration of free amino acid in the amino acid liquid is more than or equal to 100g/L, and the composite protease is prepared from alkaline protease and neutral protease according to a mass ratio of 1 (1.2-1.5).
  4. 4. The method for preparing the multifunctional biological source liquid medical fertilizer according to claim 1, wherein the step S3 is characterized in that the chitin residue and 8.5-11 mol/L potassium hydroxide solution are mixed according to a solid-to-liquid ratio of 1g (14-16) mL, the mixture is reacted for 4-5 hours at 95-98 ℃, then the second precipitate and the second liquid are obtained through solid-liquid separation, deionized water is adopted to wash the second precipitate until the pH value of washing water is 7.2-7.8, then the second precipitate is dried to obtain chitosan residue, and the second liquid and washing water are recovered and mixed to obtain potassium hydroxide residual liquid.
  5. 5. The preparation method of the multifunctional biological source liquid medical fertilizer according to claim 1 is characterized in that the step S4 is specifically characterized in that chitosan and acetic acid solution with the weight percent of 1 g/20 mL are mixed according to the solid-to-liquid ratio, the pH value is regulated to 5.0-5.5, then chitosan hydrolase with the activity of 1000-5000U/g, which is equivalent to the mass of 1.0-3.0%, is added, enzymolysis is carried out for 5-6 hours under the conditions of 48-52 ℃ and the stirring speed of 180-200 rpm, the temperature is raised to 85 ℃ and the temperature is kept for 20min to inactivate enzymes, then 5-10 kDa ultrafiltration membrane is adopted for filtration, and the chitosan oligosaccharide liquid is obtained, wherein the chitosan oligosaccharide content of the chitosan oligosaccharide liquid is more than or equal to 40g/L.
  6. 6. The preparation method of the multifunctional biological source liquid medical fertilizer according to claim 1 is characterized in that the step S5 is specifically characterized in that lignite is crushed and sieved, the lignite is dried for 2-2.5 h at 105-110 ℃ to obtain brown coal dust, the brown coal dust and potassium hydroxide residual liquid are mixed according to a solid-to-liquid ratio of 1g (5-15) mL, stirring is carried out for 2-2.5 h at 80-85 ℃ to obtain potassium fulvate crude liquid, the pH of the potassium fulvate crude liquid is regulated to 1.8-2.7 by adopting the nitric acid residual liquid, third precipitate and third liquid are obtained by filtering after stirring and standing, the pH of the third liquid is regulated to 7.5-8.0 by adopting the potassium hydroxide residual liquid, and the potassium fulvate pure liquid is obtained after concentration, wherein the content of fulvic acid in the potassium fulvate pure liquid is more than or equal to 40g/L.
  7. 7. The preparation method of the multifunctional biological source liquid medical fertilizer according to claim 1 is characterized in that in the step S6, the amino acid liquid, the chitosan oligosaccharide liquid and the potassium fulvate pure liquid are mixed according to a preset proportion, specifically, the amino acid liquid, the chitosan oligosaccharide liquid and the potassium fulvate pure liquid are mixed according to a volume ratio of (10-20): (40-80), and the buffer is a citric acid-sodium citrate buffer with a weight ratio of 0.3-0.8%.
  8. 8. The preparation method of the multifunctional biological source liquid medical fertilizer according to claim 1, wherein the step S6 is further characterized by adding avermectin microcapsules with the mass of 1.0% -5.0% and surfactant with the mass of 0.2% -0.5% into the premix, wherein the drug loading rate of the avermectin microcapsules is greater than or equal to 70%, and the surfactant consists of tween 80 and span 80 according to the mass ratio of 2:1.
  9. 9. The multifunctional biological source liquid medical fertilizer is characterized by being prepared by the preparation method of any one of claims 1-8.

Description

Multifunctional biological source liquid medical fertilizer and preparation method thereof Technical Field The invention belongs to the technical field of fertilizers, and particularly relates to a multifunctional biological source liquid medical fertilizer and a preparation method thereof. Background With the advancement of sustainable development of agriculture, the problems of soil degradation, environmental pollution, agricultural product safety and the like caused by excessive use of traditional fertilizers and pesticides are increasingly prominent, and development of multifunctional green pesticide fertilizer products with both nutrition supply and ecological safety is urgently needed. At present, the recycling of agricultural wastes becomes a research hot spot, for example, shrimp shells are rich in protein and chitin, but the existing extraction process is usually only aimed at a single component, so that the resource utilization rate is low, and the acid-base waste liquid generated in the process is easy to cause secondary pollution. Meanwhile, although the technology of compounding biological hormone and biological pesticide has been explored, the components are mostly dependent on independent sources, the production cost is high, and the systematic collaborative design is lacking. In addition, the recycling technology of the production waste liquid is still immature, and the treatment of a large amount of acid-base waste liquid increases environmental burden and production cost. Therefore, how to realize the high-value conversion of all components of agricultural wastes through an integrated process, and circularly use the waste liquid for extraction and compounding of active ingredients, develop the efficient and environment-friendly multifunctional liquid medical fertilizer, and become a key problem to be broken through in the current agricultural technical field. Disclosure of Invention In order to solve the technical problems, the invention provides a preparation method of a multifunctional biological source liquid medical fertilizer, which comprises the following steps: S1, mixing and stirring shrimp shell powder and dilute nitric acid, carrying out solid-liquid separation to obtain a first precipitate and a first liquid, washing the first precipitate by deionized water, mixing and soaking the washed first precipitate and ethanol, drying to obtain decalcified and defatted shrimp shell powder, and recovering the first liquid and washing water and mixing to obtain a residual nitric acid solution; S2, carrying out enzymolysis on the decalcified and defatted shrimp shell powder by adopting compound protease, and carrying out solid-liquid separation to obtain amino acid liquid and chitin residues; s3, mixing the chitin residue with potassium hydroxide for reaction, carrying out solid-liquid separation to obtain a second precipitate and a second liquid, washing the second precipitate by adopting deionized water, drying the second precipitate to obtain chitosan residue, recovering the second liquid and washing water, and mixing to obtain potassium hydroxide residual liquid; S4, carrying out enzymolysis on the chitosan residues by adopting chitosan hydrolase, and filtering by an ultrafiltration membrane to obtain chitosan oligosaccharide liquid; S5, crushing lignite, sieving and drying to obtain brown coal dust, mixing and stirring the brown coal dust and the potassium hydroxide residual liquid, performing solid-liquid separation to obtain a potassium fulvate crude liquid, adding the nitric acid residual liquid into the brown coal dust, stirring and filtering to obtain potassium humate precipitate and third liquid, and regulating the pH value of the third liquid to 7.5-8.0 by adopting the potassium hydroxide residual liquid to obtain a potassium fulvate pure liquid; S6, mixing the amino acid liquid, the chitosan oligosaccharide liquid and the potassium fulvate pure liquid according to a preset proportion, adding a buffering agent, uniformly stirring to obtain a premix, adding an avermectin microcapsule and a surfactant, and stirring and filtering to obtain the multifunctional biological source liquid medical fertilizer. The method comprises the steps of S1, mixing and stirring shrimp shell powder and 0.8-1.2 mol/L nitric acid according to a solid-to-liquid ratio of 1g (12-15) mL for 1-1.5 h, obtaining a first precipitate and a first liquid after solid-liquid separation, washing the first precipitate by deionized water until the pH value of washing water is 6-6.5, mixing and soaking the washed first precipitate and 95wt% ethanol according to a solid-to-liquid ratio of 1g (8-10) mL for 1-1.5 h, drying to obtain decalcified defatted shrimp shell powder, and recovering the first liquid and the washing water and mixing to obtain a nitric acid residual liquid; The step S2 is specifically that (20-25) mL of decalcified and defatted shrimp shell powder is mixed with deionized water according to a solid-to-l