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CN-122010622-A - Nutrient regulator based on bioactive substances and preparation method thereof

CN122010622ACN 122010622 ACN122010622 ACN 122010622ACN-122010622-A

Abstract

The invention provides a nutrition regulator based on bioactive substances and a preparation method thereof, wherein the nutrition regulator based on bioactive substances comprises, by weight, 10-20 parts of zymosan, 15-25 parts of fulvic acid, 20-30 parts of a high-activity carbon source, 5-10 parts of a compound biological enzyme, 8-15 parts of algae growth factors, 8-12 parts of compound probiotics, 3-8 parts of betaine, 15-25 parts of compound mineral elements and 5-10 parts of compound trace elements. The nutrient regulator disclosed by the invention is synergistic by the algae auxin formed by compounding the undaria pinnatifida fermentation product, the pomegranate rind fermentation product and the enteromorpha fermentation product, wherein the undaria pinnatifida fermentation product promotes the growth of diatoms, the enteromorpha fermentation product promotes the growth of green algae, and the pomegranate rind fermentation product can inhibit the growth of harmful algae such as blue algae, so that the proportion of beneficial algae such as diatoms is increased, the algae is maintained for a long time, stable, efficient and high-quality water color favorable for cultivation is formed, and the productivity structure of a pond is fundamentally optimized.

Inventors

  • XIE WEIXUAN
  • CHEN ZHAOSHAN

Assignees

  • 海南卓越生物有限公司

Dates

Publication Date
20260512
Application Date
20260210

Claims (10)

  1. 1. A nutrition regulator based on bioactive substances is characterized by comprising the following raw materials, by weight, 10-20 parts of zymosan, 15-25 parts of fulvic acid, 20-30 parts of a high-activity carbon source, 5-10 parts of a compound biological enzyme, 8-15 parts of algae auxin, 8-12 parts of a compound probiotics, 3-8 parts of betaine, 15-25 parts of a compound mineral element and 5-10 parts of a compound trace element; The algae auxin consists of undaria pinnatifida fermentation product, pomegranate rind fermentation product and enteromorpha fermentation product in a mass ratio of 4-6:3-5:1-2.
  2. 2. A bioactive substance-based nutritional modulator according to claim 1, characterized in that the high-activity carbon source consists of glucose and sucrose in a mass ratio of 1.0:1.0-1.5.
  3. 3. A bioactive substance-based nutritional modulator according to claim 1, wherein the complex biological enzyme consists of protease, amylase and cellulase in a mass ratio of 5-7:2-4:1-2, and the protease consists of neutral protease and alkaline protease in a mass ratio of 7-8:2-3.
  4. 4. The nutrition regulator based on bioactive substances as set forth in claim 1, wherein the fermentation product is prepared by pulverizing pollution-free and mildew-free dried Undaria pinnatifida to 60-80 mesh to obtain Undaria pinnatifida powder, mixing Bacillus subtilis, bacillus licheniformis and Lactobacillus plantarum at a mass ratio of 1.8-2.2:2.0-2.5:0.8-1.2 to obtain a compound starter I, adding 35-37% (g/mL) water at a feed liquid ratio of 1:6-10, adding 1.0-1.5 times of 4-6% brown sugar solution of the compound starter I, activating for 25-35min to obtain an activated compound starter I, mixing Undaria pinnatifida powder, brown sugar, yeast extract, potassium dihydrogen phosphate and purified water at a mass ratio of 10-15:1-2:0.3-0.5:0.1:80-90, stirring to obtain a compound starter I, adding the compound starter I to a fermentation starter at a volume ratio of 1:6-10, heating the compound starter I to a vacuum concentration of 1-3:6-37 ℃ until the compound starter I reaches a vacuum concentration of 5-40 ℃ until the temperature is equal to 60-60 MPa, concentrating the mixed starter I, concentrating under a vacuum condition until the mixed starter I reaches a vacuum condition of 1-40 ℃ until the vacuum condition is equal to 60-40.06 times, concentrating the mixed starter I until the mixed starter I reaches a vacuum condition is equal to a vacuum condition of the vacuum condition, concentrating the mixed starter I until the mixed starter I reaches a vacuum condition is equal to a vacuum condition of 60-40.0-40-60 h after the vacuum condition, and the vacuum condition is kept until the mixed starter is equal to 60-60%, drying, pulverizing, sieving with 60-100 mesh sieve to obtain fermented product of Undaria pinnatifida, wherein the protective agent I comprises maltodextrin, beta-cyclodextrin and soluble starch with mass ratio of 6.0-8.0:1.5-2.5:0.8-1.2.
  5. 5. The nutrition regulator based on bioactive substances according to claim 1, wherein the pericarpium Granati ferment is prepared by selecting pollution-free and mildew-free dry pericarpium Granati, and pulverizing to 40-60 mesh to obtain pericarpium Granati powder; compounding bacillus subtilis, aspergillus niger and lactobacillus plantarum according to the mass ratio of 2.5-3:0.9-1.1:0.8-1.0 to obtain a compound fermentation agent II, adding water with the temperature of 30-35 ℃ according to the feed liquid ratio of 1:6-10, adding brown sugar solution with the concentration of 4-6% and the mass ratio of 1.0-1.5 times that of the compound fermentation agent II into the mixture, and activating the mixture for 40-50min to obtain an activated compound fermentation product II; mixing pericarpium Granati powder, brown sugar, yeast extract, potassium dihydrogen phosphate, ammonium sulfate and purified water according to the mass ratio of 8-12:2-3:0.5-0.8:0.1:0.05:85-95, stirring uniformly to obtain a fermentation material II, adding 2-4% of activated composite fermentation material II into the fermentation material II, stirring uniformly, fermenting at 29-31deg.C for 96-120h, stirring at 40-60rpm for 24-6 min, standing for fermentation, heating to 80-85deg.C and maintaining for 15-20min, separating solid and liquid, concentrating filtrate at 50-55deg.C to 30-40% of original volume to obtain a concentrated solution II, adding 1.5-2.0 times of protective agent II into the concentrated solution II, stirring uniformly, homogenizing at 30-40MPa for 2-3 times, vacuum belt heating at 60-70 deg.C and vacuum degree of-0.06-0.08 MPa, drying, pulverizing, sieving with 60-100 mesh sieve to obtain pericarpium Granati ferment, wherein the protective agent I comprises maltodextrin, beta-cyclodextrin and soluble starch with mass ratio of 5.0-7.0:2.0-3.0:0.8-1.2.
  6. 6. The nutrition regulator based on bioactive substances as claimed in claim 1, wherein the enteromorpha fermentation is prepared by selecting dried and non-rotted dried enteromorpha, and pulverizing to 80-100 mesh to obtain enteromorpha powder; compounding bacillus subtilis, bacillus belicus and candida utilis according to the mass ratio of 1.8-2.2:1.4-1.6:0.8-1.0 to obtain a compound fermentation agent III, adding water with the temperature of 30-32 ℃ according to the feed liquid ratio of 1:6-10, adding brown sugar solution with the concentration of 4-6% and the mass ratio of 1.0-1.5 times that of the compound fermentation agent III according to the feed liquid ratio of g/mL, and activating for 55-65min to obtain an activated compound fermentation product III; mixing Enteromorpha powder, brown sugar, potassium dihydrogen phosphate, ammonium sulfate and purified water according to the mass ratio of 15-20:1.0-1.5:0.2:0.3-0.5:0.5-1.0:75-85, stirring uniformly to obtain a fermentation material III, adding 3-5% of activated composite fermentation material III into the fermentation material III, stirring uniformly, fermenting at 27-29 ℃ for 72-96h, stirring and fermenting at 40-60rpm for 20-30min every 8h, ventilating for 8-12min every 12h, maintaining stirring, heating to 80-85 ℃ and maintaining for 15-20min, performing solid-liquid separation, collecting filtrate, concentrating the filtrate to 40-50% of original volume at 52-58 ℃ to obtain a concentrated solution III, adding 1.2-1.8 times of protective agent III into the concentrated solution III, stirring and mixing uniformly, homogenizing for 2-3 times under 30-40MPa pressure, heating under vacuum condition under heating under a heating plate of 62-68 ℃ for 62 ℃, vacuum degree is-0.06 to-0.08 MPa, drying, crushing, sieving with a 60-100 mesh sieve to obtain enteromorpha fermentation product, wherein the protective agent III consists of maltodextrin, beta-cyclodextrin and soluble starch in a mass ratio of 7.0-8.0:1.0-1.5:1.0-1.5.
  7. 7. A bioactive substance-based nutritional modulator according to claim 1, wherein the complex probiotics consist of bacillus subtilis, bacillus licheniformis and photosynthetic bacteria in a mass ratio of 3.5-4.5:3.0-4.0:2.0-3.0.
  8. 8. The nutrition regulator based on bioactive substances as claimed in claim 1, wherein the composite mineral elements consist of urea, potassium dihydrogen phosphate, potassium sulfate and sodium silicate in a mass ratio of 8-12:6-9:4-6:3-5, and the composite trace elements consist of sodium iron EDTA, manganese EDTA, zinc EDTA and selenomethionine in a mass ratio of 4.5-5.5:2.0-3.0:1.5-2.5:0.1-0.3.
  9. 9. A method for the preparation of a bioactive substance-based nutritional modulator according to claim 1, comprising the steps of: S1, uniformly stirring and mixing a composite mineral element, a composite trace element, a high-activity carbon source and fulvic acid to obtain a mixture I; S2, adding zymosan, composite biological enzyme, algal auxin and betaine into the mixture I, and uniformly stirring and mixing to obtain a mixture II; And S3, adding the composite probiotics into the mixture II, stirring and mixing uniformly, and then packaging to obtain the nutrition regulator.
  10. 10. The method for preparing a bioactive substance-based nutritional regulator according to claim 9, wherein the stirring speed in S1 is 150-250rpm and the stirring time is 15-25min, the stirring speed in S2 is 80-150rpm and the stirring time is 20-30min, and the stirring speed in S3 is 60-100rpm and the stirring time is 8-15min.

Description

Nutrient regulator based on bioactive substances and preparation method thereof Technical Field The invention relates to the technical field of aquaculture water quality regulation and control, in particular to a preparation method of a nutrition regulator based on bioactive substances, which is suitable for algae cultivation, water quality optimization and pest control of sea and fresh water aquaculture water bodies. Background In aquaculture, the stability of the water environment directly affects the growth and survival rate of the farmed animals. The traditional fertilizer-water mode is mostly dependent on chemical fertilizers (such as urea and superphosphate) or single organic fertilizers, and has the problems of poor water solubility, short fertilizer efficiency, unbalanced algae phases and the like: poor water solubility, the traditional fertilizer is easy to precipitate, so that the bottom organic matters are accumulated, and the bottom is deteriorated; The fertilizer efficiency is short, the nutrition release is quick, the duration is short, the frequent fertilizer supplementation is needed, and the cultivation cost is increased; unbalanced algae phase, namely lacking directional regulation capability, is easy to cause the explosion of harmful algae such as blue algae, euglena and the like, and simultaneously, the growth of moss leads to the decrease of the light transmittance of water body, thereby influencing the growth of aquatic weeds and beneficial algae; Therefore, development of a nutrition regulator which has good water solubility, lasting fertilizer efficiency, can directionally culture beneficial algae and inhibit harmful organisms becomes an urgent need in the field of aquaculture. Disclosure of Invention In view of the above, the present invention proposes a method for preparing a bioactive substance-based nutritional regulator to solve the above-mentioned problems. The technical scheme of the invention is realized as follows: The nutrition regulator based on bioactive substances comprises, by weight, 10-20 parts of zymosan, 15-25 parts of fulvic acid, 20-30 parts of a high-activity carbon source, 5-10 parts of a compound biological enzyme, 8-15 parts of algae growth hormone, 8-12 parts of a compound probiotic, 3-8 parts of betaine, 15-25 parts of a compound mineral element and 5-10 parts of a compound trace element; Further, the algae auxin is composed of undaria pinnatifida fermentation product, pomegranate rind fermentation product and enteromorpha fermentation product in a mass ratio of 4-6:3-5:1-2. Further, the high-activity carbon source consists of glucose and sucrose with the mass ratio of 1.0:1.0-1.5. Further, the composite biological enzyme consists of protease, amylase and cellulase in the mass ratio of 5-7:2-4:1-2. Further, the protease consists of neutral protease and alkaline protease with the mass ratio of 7-8:2-3. Further, the fermented product of Undaria pinnatifida is prepared by selecting dry Undaria pinnatifida without pollution and mildew, and pulverizing to 60-80 mesh to obtain Undaria pinnatifida powder; compounding bacillus subtilis, bacillus licheniformis and lactobacillus plantarum according to the mass ratio of 1.8-2.2:2.0-2.5:0.8-1.2 to obtain a compound starter I, adding water with the temperature of 35-37 ℃ according to the feed liquid ratio of 1:6-10, adding brown sugar solution with the concentration of 4-6% and 1.0-1.5 times of the mass of the compound starter I according to the feed liquid ratio of g/mL, and activating for 25-35min to obtain an activated compound starter I; mixing Undaria pinnatifida powder, brown sugar, yeast extract, potassium dihydrogen phosphate and purified water according to the mass ratio of 10-15:1-2:0.3-0.5:0.1:80-90, stirring uniformly to obtain a fermentation material I, adding 1-3% of activated composite fermentation material I into the fermentation material I, stirring uniformly, fermenting at 33-37 ℃ for 48-72h, stirring and fermenting for the first 12h at 40-60rpm, standing and fermenting for 10-15min every 12h, heating to 80-85 ℃ and maintaining for 15-20min after fermentation, performing solid-liquid separation, collecting filtrate, concentrating the filtrate to the original volume of 35-50% at 50-60 ℃ to obtain a concentrated solution I, adding 1.5-2.0 times of protective agent I into the concentrated solution I, stirring and mixing uniformly, homogenizing for 2-3 times under 30-40MPa, performing vacuum belt heating at 65-75 ℃, vacuum degree of-0.06-0.08 MPa, drying and sieving with a heating plate to obtain the Undaria pinnatifida fermented product after drying and sieving to obtain the Undaria pinnatifida product. Furthermore, the protective agent I consists of maltodextrin, beta-cyclodextrin and soluble starch in the mass ratio of 6.0-8.0:1.5-2.5:0.8-1.2. Further, the pericarpium Granati ferment is prepared by selecting pollution-free and mildew-free dry pericarpium Granati, and pulverizing to 40-60 mesh to obtain p