CN-122010888-A - Green method for extracting ginkgo flavone from ginkgo

Abstract

The invention relates to the technical field of plant functional components and provides a green method for extracting ginkgo flavone from ginkgo, which comprises the following steps of drying and crushing ginkgo leaves to obtain ginkgo leaf dry powder; mixing folium Ginkgo dry powder with deep eutectic solvent extractant, extracting, centrifuging to obtain extractive solution, adsorbing and purifying the extractive solution to obtain ginkgo flavone eluate, concentrating and drying the ginkgo flavone eluate to obtain ginkgo flavone product, wherein the deep eutectic solvent extractant comprises N, N-dimethyl isopropanolamine, propylene glycol methyl ether and water. By the technical scheme, the problem of low yield of ginkgetin in the related technology is solved.

Inventors

• Zheng jingmei
• SHAO DEMIN
• MA CHAO
• YANG MINGYU
• ZHANG YIMENG

Assignees

• 河北瑞龙生物科技有限公司

Dates

Publication Date

20260512

Application Date

20260129

Claims (10)

1. 1.A green method for extracting ginkgo flavone from ginkgo, which is characterized by comprising the following steps: S1, drying and crushing ginkgo leaves to obtain ginkgo leaf dry powder; S2, mixing the ginkgo leaf dry powder with a deep eutectic solvent extractant, extracting, and centrifuging to obtain an extracting solution; s3, adsorbing and purifying the extracting solution to obtain ginkgo flavone eluent; S4, concentrating and drying the ginkgo flavone eluent to obtain a ginkgo flavone product; the deep eutectic solvent extractant comprises N, N-dimethyl isopropanolamine, propylene glycol methyl ether and water.
2. 2. The green method for extracting ginkgo flavone from ginkgo biloba according to claim 1, wherein the molar ratio of the N, N-dimethyl isopropanolamine to the propylene glycol methyl ether is 1-2:1.
3. 3. The green method for extracting ginkgo flavone from ginkgo biloba as claimed in claim 1, wherein the preparation method of the deep eutectic solvent extractant comprises the following steps of mixing N, N-dimethyl isopropanolamine, propylene glycol methyl ether and water until the mixture becomes transparent liquid, so as to obtain the deep eutectic solvent extractant.
4. 4. A green method for extracting ginkgo flavone from ginkgo biloba as claimed in claim 3, wherein the mass of water in the deep eutectic solvent extractant is 10% -30% of the sum of the mass of N, N-dimethyl isopropanolamine and propylene glycol methyl ether.
5. 5. A green method for extracting ginkgo flavone from ginkgo biloba as claimed in claim 3, wherein the mixing temperature is 80-90 ℃.
6. 6. The green method for extracting ginkgo flavone from ginkgo biloba as claimed in claim 1, wherein in the step S3, the adsorption and purification comprises the steps of adsorbing the extracting solution by using macroporous adsorption resin I, eluting by using eluent I after water leaching, collecting the eluent, adsorbing by using macroporous adsorption resin II, eluting by using eluent II after water leaching, and obtaining ginkgo flavone eluent, wherein the macroporous adsorption resin I and the macroporous adsorption resin II are one or two of HPD-600 resin and AB-8 resin respectively and independently.
7. 7. The green method for extracting ginkgo flavone from ginkgo biloba as claimed in claim 6, wherein the macroporous adsorption resin I is HPD-600 macroporous adsorption resin, and the macroporous adsorption resin II is AB-8 macroporous adsorption resin.
8. 8. The green method for extracting ginkgo flavone from ginkgo biloba according to claim 1, wherein in the step S2, the volume ratio of the ginkgo biloba dry powder to the deep eutectic solvent extractant is 1 g:8-12 ml.
9. 9. The green method for extracting ginkgo flavone from ginkgo biloba according to claim 1, wherein in the step S2, the extraction temperature is 40-50 ℃ and the extraction time is 30-60 min.
10. 10. The green method for extracting ginkgo flavone from ginkgo biloba according to claim 1, wherein in the step S2, the centrifugal relative centrifugal force is 10000-12000 g, and the time is 15-25 min.

Description

Green method for extracting ginkgo flavone from ginkgo Technical Field The invention relates to the technical field of extraction of plant functional components, in particular to a green method for extracting ginkgo flavone from ginkgo. Background The ginkgo flavone is a special polyphenol active substance of ginkgo, mainly comprises monomer components such as quercetin, kaempferol, isorhamnetin and the like, has excellent antioxidant, anti-inflammatory, microcirculation improving and nerve cell protecting physiological activities, has irreplaceable application value in the fields of clinical medicine, functional health care products, high-end cosmetics, food additives and the like, and along with the rapid development of health industry, the market demand for high-purity ginkgo flavone climbs year by year, and promotes the iterative upgrade of related extraction technologies. At present, the traditional technology for industrially extracting ginkgo flavone is mainly an organic solvent extraction method, assisted by a water extraction method, a microwave assisted extraction method and the like, wherein the organic solvent extraction method is usually used for reagents such as ethanol, acetone, ethyl acetate and the like, and has the defects of a plurality of green and environment-friendly layers, such as volatile organic pollutants formed by the solvent which are easy to volatilize and pollute the atmosphere, toxicity of part of the solvent which is easy to remain in a product and influence the safety of the product, repeated distillation is needed for solvent recovery, high energy consumption and limited recovery efficiency, and the total production cost is increased. Although the water extraction method has the advantages of green solvent and no pollution, the extraction efficiency is low due to moderate polarity of ginkgo flavone and lower solubility in water, the content of impurities in the extracting solution is high, the subsequent separation and purification steps are complicated, the production difficulty and the cost are further increased, and the traditional technology is difficult to meet the development requirements of low pollution, low energy consumption and high safety of the current green chemical industry. In order to solve the green problem of the traditional extraction technology, the deep eutectic solvent is gradually applied to the field of ginkgo flavone extraction. The solvent is prepared by mixing a hydrogen bond acceptor such as choline chloride and a hydrogen bond donor such as glycerol according to a specific molar ratio and forming a eutectic mixture through strong intermolecular hydrogen bond action, has the remarkable green characteristics of simple preparation process, no need of complex purification steps, low toxicity, biodegradability, low vapor pressure, no volatile pollution, adjustable property, different target products and the like, can effectively overcome the environmental protection and safety defects of the traditional organic solvent, and is considered as an ideal scheme for extracting ginkgo flavone instead of the traditional solvent. However, in the existing ginkgo flavone extraction technology based on deep eutectic solvent, the extraction yield of ginkgo flavone is generally low due to insufficient optimization of the deep eutectic solvent system ratio and low matching degree of extraction process parameters, so that the efficiency requirement of industrialized mass production is difficult to meet, and the industrialized application of the green extraction technology is restricted. Therefore, a green method for extracting ginkgo flavone from ginkgo needs to be proposed. Disclosure of Invention The invention provides a green method for extracting ginkgo flavone from ginkgo, which solves the problem of low ginkgo flavone yield in the related technology. The technical scheme of the invention is as follows: The invention provides a green method for extracting ginkgo flavone from ginkgo, which comprises the following steps: S1, drying and crushing ginkgo leaves to obtain ginkgo leaf dry powder; S2, mixing the ginkgo leaf dry powder with a deep eutectic solvent extractant, extracting, and centrifuging to obtain an extracting solution; s3, adsorbing and purifying the extracting solution to obtain ginkgo flavone eluent; S4, concentrating and drying the ginkgo flavone eluent to obtain a ginkgo flavone product; the deep eutectic solvent extractant comprises N, N-dimethyl isopropanolamine, propylene glycol methyl ether and water. As a further technical scheme, the molar ratio of the N, N-dimethyl isopropanolamine to the propylene glycol methyl ether is 1-2:1. The preparation method of the deep eutectic solvent extractant comprises the following steps of mixing N, N-dimethyl isopropanolamine and propylene glycol methyl ether with water until the mixture becomes transparent liquid, and obtaining the deep eutectic solvent extractant. As a further technical scheme, in the d