CN-122011065-A - Method for removing anthocyanin from dendrobium candidum and application

CN122011065ACN 122011065 ACN122011065 ACN 122011065ACN-122011065-A

Abstract

The invention relates to the technical field of traditional Chinese medicines, in particular to a method for removing anthocyanin from dendrobium candidum and application thereof. The invention aims to solve the technical problems that the color of the traditional dendrobium candidum product is mostly purple and the color depth is different, so that the cognition of consumers is affected. The technical scheme includes that fresh dendrobium candidum strips are added into an enzymolysis solution for enzymolysis, enzyme inactivation is carried out after the enzymolysis is finished to obtain fresh dendrobium candidum strips subjected to enzymolysis, the fresh dendrobium candidum strips subjected to enzymolysis are added into an ethanol solution for soaking, and filtration is carried out to obtain filtrate containing anthocyanin and fresh dendrobium candidum strips from which anthocyanin is removed. The invention has the main purposes of efficiently removing anthocyanin in dendrobium candidum, so that the processed product is kept natural green, the color change problem is inhibited from the source, and the developed product can be ensured to keep the original green of dendrobium candidum stalks.

Inventors

TIAN YINGQIU
GAO MINGJU
ZHOU CHENGYAN
Tong Shaxin
LUO YANG
MAO ZHONGSHUN
AO JINCHENG
ZHOU JIAMING

Assignees

文山学院

Dates

Publication Date: 20260512
Application Date: 20260130

Claims (10)

1. The method for removing the anthocyanin from the dendrobium candidum is characterized by comprising the following steps of: s1, adding fresh dendrobium candidum strips into an enzymolysis solution for enzymolysis; s2, after enzymolysis is finished, enzyme inactivation is carried out to obtain the fresh dendrobium candidum strips subjected to enzymolysis treatment; S3, adding the fresh dendrobium candidum strips subjected to enzymolysis treatment into an ethanol solution, soaking, and filtering to obtain filtrate containing anthocyanin and the fresh dendrobium candidum strips from which the anthocyanin is removed.
2. The method for extracting anthocyanin from dendrobium candidum according to claim 1, wherein in S1, the enzymolysis solution comprises cellulase, pectase and water, the dosage ratio of the fresh dendrobium candidum to the cellulase to the pectase to the water is 9 g:0.5-1 g:100mL, the enzymolysis time is 30-90 min, and the enzymolysis temperature is 55 ℃; And/or S2 comprises heating to 90deg.C after enzymolysis, and maintaining for 5min to inactivate enzyme to obtain fresh strips of Dendrobium officinale processed by enzymolysis; and/or in S3, the ethanol solution is the ethanol water solution, the concentration of ethanol in the ethanol water solution is 0-100%, and the soaking time is 7-9 h.
3. The method for extracting the anthocyanin from the dendrobium candidum according to claim 2, wherein in S1, the dosage ratio of the fresh dendrobium candidum to the cellulase to the pectase to the water is 9g to 1g to 100mL, the enzymolysis time is 30min, and the enzymolysis temperature is 55 ℃; And/or, in S3, the concentration of ethanol in the ethanol water solution is 40%, and the soaking time is 7h.
4. The method of claim 1, wherein the anthocyanin-removed fresh dendrobium candidum strips are used for preparing dendrobium candidum primary pulp.
5. The use according to claim 4, wherein the preparation method of the dendrobium candidum magma comprises the following steps: Step A1, soaking dried lily, and then mixing the soaked dried lily with the fresh dendrobium candidum strips separated from anthocyanin, and completely crushing to obtain primary liquid; Step A2, filtering the primary liquid to remove residual dendrobium candidum fibers, and obtaining first iron dendrobium candidum primary pulp; and A3, heating first iron dendrobium candidum primary pulp, adding fructose and potassium sorbate, and shaking uniformly to obtain dendrobium candidum primary pulp.
6. The use according to claim 5, wherein in the step A1, the step of soaking dried lily is to add 200mL of clear water into 6g of dried lily, and soak for 12 hours; and/or, in the step A2, a 100 mesh screen is adopted for filtering; And/or, in the step A3, the temperature of the heating treatment is 90-95 ℃ and the time of the heating treatment is 30min; and/or, the dosage ratio of first iron to 9mL of the dendrobium candidum primary pulp to the fructose to the potassium sorbate is 40mL to 0.01g; and/or shaking by a shaking table, wherein the rotation speed of the shaking table is 100-150 r/min, and the time is 8-12 h.
7. The method of claim 1, wherein the anthocyanin-removed fresh dendrobium candidum strips are used for preparing dendrobium candidum beverage.
8. The use according to claim 7, wherein the preparation method of the dendrobium candidum beverage comprises the following steps: Step B1, performing first crushing treatment on the fresh dendrobium candidum strips from which the anthocyanin is removed until the stems of the dendrobium candidum are completely crushed to obtain primary liquid I; Step B2, performing first filtering treatment on the primary liquid I to remove residual dendrobium candidum fibers to obtain primary filtrate, heating the primary filtrate, and cooling to obtain cooled primary filtrate; Step B3, mixing the cactus fruit with the cooled primary filtrate, and performing second crushing treatment until the cactus fruit is completely crushed to obtain a primary liquid II; Step B4, performing second filtering treatment on the primary liquid II to remove residual cactus fruit impurities and obtain green filtrate; step B5, adding tremella polysaccharide and potassium sorbate into the green filtrate, sealing, and shaking uniformly to obtain dendrobium candidum juice; And B6, preliminarily cooling the dendrobium candidum juice, freezing to obtain dendrobium candidum juice ice cubes, and crushing, melting and third filtering the dendrobium candidum juice ice cubes to obtain the dendrobium candidum beverage.
9. The application of claim 8, wherein in the step B1, the specific step of the first crushing treatment comprises the steps of putting the fresh dendrobium candidum strips from which anthocyanin is removed into a stirrer, adding water, controlling the rotating speed to be 32000r/min, and stirring for 2-3 min; and/or, in the step B2, a 40-mesh screen is adopted for the first filtering treatment, the temperature of the primary filtrate is 90-95 ℃, and the heating treatment time is 30min; the proportion of the fresh dendrobium candidum strips from which anthocyanin is removed, water and the primary filtrate after cooling is 9g:300mL:190mL; and/or in the step B3, the dosage ratio of the cactus fruit to the cooled primary filtrate is 50g:190mL, the rotating speed of the second crushing treatment is 32000r/min, and the time is 2-3 min; And/or, in the step B4, performing the second filtering treatment by using a 100 mesh screen; And/or in the step B5, shaking by a shaking table, wherein the rotation speed of the shaking table is 100-150 r/min, and the time is 8-12 h; And/or, in the step B5, the ratio of the green filtrate to the tremella polysaccharide to the potassium sorbate is 220mL:10mL:0.3g; and/or in the step B6, the temperature of the primary cooling is 0-4 ℃, the freezing temperature is-25 ℃, the freezing time is more than or equal to 6 hours, and a 100-mesh screen is adopted for the third filtering treatment.
10. Use of the anthocyanin-containing filtrate obtained by the method of claim 1 in an 18 ° beauty liquor, wherein the 18 ° beauty liquor exhibits a light pink or purplish color.

Description

Method for removing anthocyanin from dendrobium candidum and application Technical Field The invention relates to the technical field of traditional Chinese medicines, in particular to a method for removing anthocyanin from dendrobium candidum and application thereof. Background The dendrobium candidum is mainly developed as a raw material of the product, wherein three parts are rich in anthocyanin, and the anthocyanin is generally formed by combining anthocyanin with one or more glycosyl through glycosidic bonds. Anthocyanin is easily influenced by chemical structure, temperature, pH, illumination, oxygen and the like, so that molecules are subjected to isomerization, polymerization and degradation, and the color of anthocyanin is directly influenced. At present, the main processing raw materials of dendrobium candidum are stem strips, the stem strips are generally green before being cut and picked, the processed products taking the dendrobium candidum as the raw materials are more popular product types including dendrobium candidum magma, dendrobium candidum beverage, dendrobium candidum wine and other liquid drinks, the color of the existing dendrobium candidum liquid drinks in the market is mostly purple, and the color depth is different, so that the visual cognition of common consumers on the dendrobium candidum plant, which is green, causes a plurality of questions of whether the consumers are genuine/genuine products, whether the consumer is overdue, whether additives are too much and the like, and seriously influences the purchase intention. Therefore, natural green raw materials of the dendrobium candidum are created (which is a neck clamping problem which is not solved for a long time in the industry), green healthy products are developed by the dendrobium candidum, and the dendrobium candidum preparation method has great significance in improving green image of the dendrobium candidum product and setting up market competitiveness of the dendrobium candidum product. In addition, necessary collection or recovery, development and utilization of the dendrobium candidum anthocyanin are also important to increase the added value of the product. Disclosure of Invention Aiming at the problems existing in the prior art, the invention provides a method for removing anthocyanin from dendrobium candidum and application thereof, so as to solve the problems that the color of the existing dendrobium candidum product is mostly purple and the color depth is different, and the cognition of consumers is affected. The technical scheme of the invention is as follows: the invention provides a method for removing anthocyanin from dendrobium candidum, which comprises the following steps: s1, adding fresh dendrobium candidum strips into an enzymolysis solution for enzymolysis; s2, after enzymolysis is finished, enzyme inactivation is carried out to obtain the fresh dendrobium candidum strips subjected to enzymolysis treatment; S3, adding the fresh dendrobium candidum strips subjected to enzymolysis treatment into an ethanol solution, soaking, and filtering to obtain filtrate containing anthocyanin and the fresh dendrobium candidum strips from which the anthocyanin is removed. Through skillfully combining enzyme treatment with organic solvent ethanol treatment, anthocyanin in the dendrobium candidum can be removed efficiently, the processed product is kept natural green, the problems that the color of the existing dendrobium candidum product is mostly purple and the color depth is different and the cognition of consumers is affected are solved, the color-changing problem is inhibited from the source, and the developed product can be ensured to keep the original green of dendrobium candidum stalks. Meanwhile, the method has the effects of energy conservation, environmental protection and synergy, specifically, the enzymolysis process is carried out under mild conditions, so that the energy consumption can be greatly reduced, the ethanol used can be recycled, the solvent consumption and the waste emission are reduced, and the method accords with the green production concept; meanwhile, the enzymolysis firstly breaks the cell wall structure of the dendrobium candidum, breaks the leaching barrier, creates good conditions for subsequent ethanol leaching, obviously improves anthocyanin extraction efficiency, and realizes the technological effect of cooperative gain. In S2, the enzyme is inactivated to obtain an upper enzymatic hydrolysate. In the S1, the enzymolysis solution comprises cellulase, pectase and water, the dosage ratio of the fresh dendrobium candidum strips to the cellulase to the pectase to the water is 9g to 0.5-1 g to 100mL, the enzymolysis time is 30-90 min, and the enzymolysis temperature is 55 ℃. Further, the step S2 comprises the step of heating to 90 ℃ after the enzymolysis is finished, and maintaining for 5min to inactivate enzymes to obtain the fresh dendrobium candidum strips subjected to enzymolysis. In