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CN-122011066-A - Preparation method of exogenous indian pterocarpus santalinus glycoside and application thereof in promoting germination of corn seeds

CN122011066ACN 122011066 ACN122011066 ACN 122011066ACN-122011066-A

Abstract

The invention provides a preparation method of exogenous indian pterocarpus santalinus glycoside and application thereof in promoting germination of corn seeds, and belongs to the technical field of seed germination. The method utilizes the Indian pterocarpus santalinus glycoside extracted from chickpeas (Cicer arietinum L.) to carry out coating treatment on corn seeds, specifically, the Indian pterocarpus santalinus glycoside suspension is mixed with a film forming agent to prepare a seed coating agent to carry out uniform coating treatment on the corn seeds, the concentration of the Indian pterocarpus santalinus glycoside suspension is limited to 800-1600 mg/L, the exogenous Indian pterocarpus santalinus glycoside prepared by the method can obviously promote the germination performance of the corn seeds in a low-temperature high-humidity environment and effectively improve seedling quality, and the scheme of the invention extracts the Indian pterocarpus santalinus glycoside from plant sources, so that the method is convenient to obtain materials, low in price and environment-friendly, and fills the blank of biological stimulation for promoting corn seed germination in a cold spring environment.

Inventors

  • ZHANG YIFEI
  • ZHANG CHUNYU
  • DOU YI
  • WANG XIAOTIAN
  • HOU JUNJUN
  • YU SONG
  • LI WANGSHU
  • LUO WENQI
  • YIN JIAQI

Assignees

  • 黑龙江八一农垦大学

Dates

Publication Date
20260512
Application Date
20260203

Claims (10)

  1. 1. A method for preparing exogenous indian pterocarpus santalinus glycoside, which is characterized by comprising the following steps: step S1, germinating chickpeas, freeze-drying the germinated chickpeas, grinding the freeze-dried chickpeas, leaching with methanol at room temperature, and combining the obtained leaching solutions; step S2, evaporating and combining the obtained leaching liquor under reduced pressure to obtain a methanol crude extract, extracting the methanol crude extract, and evaporating under reduced pressure to obtain an n-butanol extract; and S3, performing silica gel column chromatography separation, purification and elution on the n-butanol extract, and finally further separating, purifying and eluting the components collected by the gradient elution system to obtain the indian pterocarpine glycoside.
  2. 2. The method for preparing exogenous pterocarpus santalinus according to claim 1, wherein the germination temperature in step S1 is 20-30 ℃, preferably 24-28 ℃, and the germination time is 2-8 d, preferably 4-6 d.
  3. 3. The preparation method of the exogenous indian pterocarpus santalinus glycoside according to claim 1, wherein the freeze-drying treatment in the step S1 is specifically that germinated chickpeas are pre-frozen for 3-5 hours at the temperature of-50 to-40 ℃, then are subjected to primary vacuum drying by gradually heating to-20 to-10 ℃ under the vacuum degree of 10-30 Pa, and finally are subjected to secondary vacuum drying by gradually heating to 20-30 ℃ under the vacuum degree of 10-20 Pa, wherein the primary vacuum drying time is 18-24 hours, and the secondary vacuum drying time is 6-8 hours.
  4. 4. The method for preparing exogenous pterocarpus santalinus according to claim 1, wherein the water content of the freeze-dried chickpeas in the step S1 is 1% -3%, and the mesh number of the ground chickpeas is 60-80 mesh.
  5. 5. The method for preparing exogenous indian pterocarpus santalinus glycoside according to claim 1, wherein the solid-to-liquid ratio of the methanol leaching in the step S1 is 1:10-15 kg/L, the number of times of the methanol leaching is 3-4, and the duration of each methanol leaching is 18-36 h.
  6. 6. The method for preparing exogenous indian pterocarpus santalinus according to claim 1, wherein the parameters of reduced pressure evaporation in step S2 are 50-100 kPa absolute, and the water bath temperature of reduced pressure evaporation is 30-35 ℃; The extraction liquid of the extraction treatment is n-butanol and water, the volume ratio of the n-butanol to the water is 1-2:1, and the dosage ratio of the methanol crude extract to the extraction liquid is 1 g:3-5 mL.
  7. 7. The method for preparing exogenous indian pterocarpus santalinus according to claim 1, wherein the silica gel column in step S3 is a 200-300 mesh column chromatography silica gel column, and the column diameter-to-height ratio of the silica gel column is 1:20; the eluting eluent is a mixed solution of ethyl acetate and methanol, and the system gradient of the elution is 100:0-90:10.
  8. 8. The use of the pterocarpin obtained by the preparation method of any one of claims 1-7 in promoting germination of corn seeds.
  9. 9. The application of the pterocarpus indicus glycoside in promoting corn seed germination according to claim 8, wherein the application refers to uniform coating treatment of corn seeds by a seed coating agent prepared by mixing a printing pterocarpus indicus glycoside suspension and a film forming agent, and the dosage relationship between the seed coating agent and the corn seeds is 2-4 mL/1 kg.
  10. 10. The application of the pterocarpin according to claim 8 in promoting corn seed germination, wherein the volume ratio of the pterocarpin suspension to the film forming agent is 3-4:1, and the concentration of the pterocarpin suspension is 800-1600 mg/L.

Description

Preparation method of exogenous indian pterocarpus santalinus glycoside and application thereof in promoting germination of corn seeds Technical Field The invention belongs to the technical field of seed germination, and particularly relates to a preparation method of exogenous indian pterocarpine glycoside and application thereof in promoting corn seed germination. Background Corn (Zea mays l.) is a C4 plant of the poaceae family, and is also an important crop type that integrates food, commercial and feed crops, and has been widely planted worldwide. As an important commercial grain production base, the corn sowing area in northeast China is about 31 percent of the whole country, the yield is about 35 percent of the whole country, and the corn sowing area plays an important role in national grain safety guarantee and national economic development strategy. Although improvement of heat conditions is generally beneficial to crop production in the global climate change background, extreme climate events and agricultural meteorological disasters have an increasing trend, along with the north-to-east extension of corn variety planting areas in different maturity periods in northeast China, the planting development trend of promoting growth period extension such as replacement of early maturity varieties by middle-late maturity varieties in sensitive areas, timely early sowing and the like, so that regional and staged low-temperature meteorological disasters from Heilongjiang to Jilin north still occur, and early (sowing to esculentus) and late (maturing to maturing) of the childbirth are taken as main occurrence stages, serious low-temperature disastrous weather generally occurs 1 time every 3-5 years on average, and yield loss can be caused to be more than 15%. Particularly, in recent years, after corns in northeast high-latitude cold regions are sowed, the frequency of occurrence of coupling of spring frost or reverse spring cold with unfavorable weather conditions such as overcast and rainy low-level illumination, waterlogging and the like is increased, so that germination and seedling formation disorders of seeds are easily caused, the emergence rate is reduced, the emergence progress is delayed, the seedling formation rate is reduced, the proportion of weak seedlings is increased, the phenomena of large seedlings and small seedlings are obvious, and even the seeds completely lose the germination force, so that large-area seedling shortage is caused, and finally, high-quality corn groups are difficult to form, so that the method becomes one of key bottleneck problems of whole-process mechanical high yield and high quality of corns in cold regions, and the high-efficiency continuous development of the corn industry in the regions is severely restricted. Since 1866, the field of agricultural production at home and abroad is well innovated by technicians in the field of seed treatment, and based on the biological characteristics of seeds of different crops, the wide range of biological and abiotic stresses possibly suffered by the sown seeds are pertinently handled through physical, chemical, biochemical and biological technical means, so that the targets of seed germination, high-quality seedling establishment and normal growth and development of plants are promoted, and finally the yield potential of the crops is fully exerted. The seed coating technology is an important agronomic measure for improving the fluidity of seeds during sowing, protecting the seeds from being infected by diseases and insect pests and promoting the smooth germination of the seeds and the normal growth and development of seedlings in the current global corn production. Since 1968 researchers succeeded in separating botanical pesticides from neem, the field of botanical pesticides has gradually entered a new stage of systematic development and scale application. The term "biostimulant" has provided better definition and classification for improving plant growth and development performance, particularly seed treatment materials, in the 21 st century. "biostimulants" are defined as a class of substances that trigger and regulate the formation of physiological and molecular processes associated with crop yield and quality, and may be natural products or biological agents. The biological hormone used as seed coating technology relates to beneficial bacteria and fungi, plant and animal derived proteins and hydrolysates thereof, amino acids, carbohydrate derivatives, seaweed and herbal extracts, vitamins, humic acid, fulvic acid and other substances, which are used as the biological hormone for seed coating, can regulate and control the growth metabolism of target plants under specific environmental conditions, and further strengthen the seed germination and seedling establishment effects. At present, the seed treatment technology is in a key period of transforming from chemical dependence to green intelligence, and in the transformation