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CN-122011086-A - Separation method of bioactive proteins in bovine coloctrum and preparation method of composite bovine coloctrum product

CN122011086ACN 122011086 ACN122011086 ACN 122011086ACN-122011086-A

Abstract

The invention relates to the technical field of dairy product separation and extraction, in particular to a separation method of bioactive proteins in bovine coloctrum and a preparation method of a compound bovine coloctrum product. The separation method of the bioactive proteins in the bovine coloctrum comprises the steps of S1, pretreating raw milk to obtain sterilized bovine coloctrum, S2, carrying out first ultrafiltration treatment on the sterilized bovine coloctrum to obtain a first permeate and a first retentate containing IgG, S3, carrying out second ultrafiltration treatment on the first permeate to obtain a second permeate and a second retentate containing lactoferrin, and S4, carrying out third ultrafiltration treatment on the second permeate to obtain a third retentate containing alkaline proteins. The separation method can not only efficiently extract IgG, but also can ensure that the high activity of lactoferrin and alkaline protein is reserved. Meanwhile, the technology can prepare the bovine coloctrum composite protein product rich in various high-activity proteins.

Inventors

  • JING XIAOXIA
  • MU ZHISHEN
  • ZHANG FULONG
  • WU YUN
  • Dou Nianxu

Assignees

  • 内蒙古蒙牛乳业（集团）股份有限公司

Dates

Publication Date
20260512
Application Date
20260106

Claims (9)

  1. 1. A method for separating bioactive proteins in bovine colostrum, comprising: s1, pretreating raw milk to obtain sterilized bovine colostrum; S2, performing first ultrafiltration treatment on the sterilized bovine coloctrum so as to obtain a first permeate and a first trapped fluid containing IgG; s3, performing second ultrafiltration treatment on the first permeate so as to obtain a second permeate and a second trapped fluid containing lactoferrin; and S4, performing third ultrafiltration treatment on the second permeate so as to obtain a third trapped fluid containing alkaline proteins.
  2. 2. The separation process of claim 1, wherein the first ultrafiltration treatment is performed by filtration and concentration using an ultrafiltration membrane having a molecular weight cut-off of 100 to 120 kDa.
  3. 3. The separation process of claim 1, wherein the second ultrafiltration treatment is performed by filtration and concentration using an ultrafiltration membrane having a molecular weight cut-off of 30 to 50 kDa.
  4. 4. The separation method according to claim 1, wherein the third ultrafiltration treatment is performed by filtration and concentration using an ultrafiltration membrane having a molecular weight cut-off of 5 to 10 kDa.
  5. 5. The separation method according to claim 1, wherein in step S1, the sterilization treatment comprises cross-flow microfiltration at 5-10 ℃ using a microfiltration membrane; Optionally, the microfiltration membrane has a pore size of 0.2-0.45 μm.
  6. 6. A method of preparing a composite bovine coloctrum product comprising: (1) Enriching and ultrafiltration concentrating the first trapped fluid to obtain concentrated fluid containing IgG, Wherein the first retentate is the first retentate obtained in the method for separating bioactive proteins in bovine coloctrum of any one of claims 1-5; (2) Mixing the concentrated solution containing IgG with a second trapped solution containing lactoferrin and a third trapped solution containing alkaline protein to obtain a composite bovine coloctrum state product, Wherein the second retentate and the third retentate are the second retentate and the third retentate obtained in the method for separating bioactive proteins in bovine coloctrum according to any one of claims 1-5.
  7. 7. The method of manufacturing according to claim 6, wherein step (1) further comprises: 1) Adjusting the pH of the first trapped fluid to 4.4-4.6 to obtain a first supernatant enriched with IgG; 2) Centrifuging the supernatant to obtain a second supernatant enriched in IgG; 3) Subjecting the second supernatant to a fourth ultrafiltration treatment so as to obtain the IgG-containing concentrate; Optionally, the temperature of the centrifugal treatment is 4-8 ℃ and the rotating speed is 5000-7000 rpm; optionally, the fourth ultrafiltration treatment is carried out by filtration and concentration using an ultrafiltration membrane having a molecular weight cut-off of 50 to 100 kDa.
  8. 8. The method of manufacturing according to claim 6, further comprising: Sterilizing the composite bovine coloctrum product; optionally, the preparation method further comprises freeze-drying the sterilized composite bovine coloctrum product to obtain a composite bovine coloctrum solid product.
  9. 9. A composite bovine colostrum product, characterized in that the composite bovine colostrum product is obtained by the process for preparing a composite bovine colostrum product as claimed in any one of claims 6 to 8.

Description

Separation method of bioactive proteins in bovine coloctrum and preparation method of composite bovine coloctrum product Technical Field The invention relates to the technical field of dairy product separation and extraction, in particular to a separation method of bioactive proteins in bovine coloctrum and a preparation method of a compound bovine coloctrum product. Background Bovine colostrum is rich in a variety of bioactive proteins, of which immunoglobulins (especially IgG), lactoferrin and basic proteins are of paramount importance. It has unique values of enhancing immunity, resisting bacteria and viruses, promoting growth, regulating physiological functions, etc. At present, the process for separating specific proteins from bovine coloctrum mainly comprises the following steps: Single IgG extraction process, common methods such as salting out, ultrafiltration, ion exchange chromatography, etc. These methods are generally aimed at achieving high purity extraction of IgG, but other active proteins are often ignored or damaged during the process. For example, severe pH changes or high salt environments can lead to irreversible denaturation of lactoferrin and alkaline proteins. The process of mixing active protein powder, typically by low temperature pasteurization and spray drying, aims to "retain" all active ingredients. However, this method cannot increase the relative content of a specific protein, and it is difficult to achieve optimal activity retention of all components due to the difference in heat sensitivity of different proteins. The chromatographic purification process can obtain high purity single protein, but has long process flow, high cost, complex equipment and great activity loss in the treatment process, and is not suitable for the mass production of functional food or health care product raw materials. However, the existing separation process has the following defects that (1) the compatibility is poor, and the existing process has difficulty in simultaneously combining the high extraction rate of IgG and the high activity retention rate of lactoferrin and alkaline proteins on one process route. IgG is often extracted, and other precious components are wasted; the method comprises the steps of (1) carrying out pH adjustment, phase change, heating and the like repeatedly to generate an accumulation effect, wherein the accumulation effect is caused to the heat-sensitive and shear-sensitive lactoferrin and alkaline proteins, (3) carrying out a complex process, and the cost is high, wherein a route for respectively extracting and re-compounding a plurality of proteins is extremely high, so that industrialization cannot be realized, and (4) the product is single in form, namely the final product is usually single IgG powder, and a compound functional ingredient with a synergistic enhancement effect cannot be formed. Therefore, there is a need to develop a new process that can extract immunoglobulins from bovine colostrum in an integrated, efficient and gentle manner while retaining the activity of lactoferrin and alkaline proteins. Disclosure of Invention The present invention aims to solve at least one of the technical problems in the related art to some extent. Therefore, an object of the present invention is to provide a method for separating bioactive proteins from bovine coloctrum and a method for preparing a composite bovine coloctrum product, which can not only efficiently extract IgG, but also retain high activity of lactoferrin and alkaline proteins. Meanwhile, the invention provides a multi-active protein co-enrichment process which is simple and convenient to operate, mild in condition and suitable for large-scale production. The bovine coloctrum composite protein product rich in various high-activity proteins can be prepared by the process. To this end, the first aspect of the invention provides a method for separating bioactive proteins in bovine colostrum. According to an embodiment of the invention, the separation method comprises: s1, pretreating raw milk to obtain sterilized bovine colostrum; S2, performing first ultrafiltration treatment on the sterilized bovine coloctrum so as to obtain a first permeate and a first trapped fluid containing IgG; s3, performing second ultrafiltration treatment on the first permeate so as to obtain a second permeate and a second trapped fluid containing lactoferrin; and S4, performing third ultrafiltration treatment on the second permeate so as to obtain a third trapped fluid containing alkaline proteins. The separation method of bioactive proteins in bovine coloctrum provided by the invention sets a 'collaborative enrichment' target, and realizes the integrated utilization of various high-value active components of bovine coloctrum by simultaneously and efficiently extracting IgG and enriching lactoferrin and alkaline proteins through one process chain. According to an embodiment of the invention, the first ultrafiltration treatment is pe