CN-122011097-A - White kidney bean polypeptide and application thereof

Abstract

The invention relates to the technical field of polypeptides, and discloses a white kidney bean polypeptide and application thereof. The amino acid sequence of the polypeptide is Asp-Asn-Pro-Phe-Tyr-Phe. The polypeptide is prepared by hydrolyzing white kidney beans through alkaline protease, and experiments prove that the polypeptide has an inhibition effect on alpha-amylase and alpha-glucosidase, so that the polypeptide can be used for preparing a medicament for reducing blood sugar and also can be used for preparing a health care product for maintaining blood sugar health level.

Inventors

• YIN JUNLIN
• CHEN JINGCHAO
• GUO YAFEI
• WANG KAIMIN
• He Zhenxiu
• SUN WEIQING
• ZHANG ZHAOLIN
• WANG YUE
• XU LILI
• FAN BAOMIN
• FAN RUIFENG
• XU JIANBIN
• ZHOU YONGYUN
• ZENG GUANGZHI

Assignees

• 云南民族大学

Dates

Publication Date

20260512

Application Date

20260129

Claims (3)

1. 1. A white kidney bean polypeptide is characterized in that the amino acid sequence of the white kidney bean polypeptide is Asp-Asn-Pro-Phe-Tyr-Phe.
2. 2. Use of the white kidney bean polypeptide of claim 1 in the preparation of a hypoglycemic agent.
3. 3. Use of the white kidney bean polypeptide of claim 1 in the preparation of a health product that helps maintain blood glucose health levels.

Description

White kidney bean polypeptide and application thereof Technical Field The invention relates to the technical field of active peptides, in particular to a white kidney bean polypeptide and application thereof. Background Currently, global diabetes mellitus incidence is increased year by year with continuous improvement of living standard and unreasonable dietary structure of people, and energy intake and consumption imbalance is one of causes of metabolic diseases such as type II diabetes mellitus and the like, acarbose is used as an alpha-glucosidase inhibitor, and core side effects are directly related to action mechanisms, namely gastrointestinal reactions such as flatulence, abdominal distension, abdominal pain and diarrhea are usually caused by delaying digestion and absorption of carbohydrates, serious adverse reactions are rare, but rare liver injury risks and hypoglycemia possibly occurring when other hypoglycemic agents are combined are required to be paid attention. White kidney beans are planted in various provinces and areas of China, and the provinces with larger planting areas are regions such as Yunnan, guizhou, sichuan and the like. The ancient medical books in China state that white kidney beans are sweet and flat in taste and have the effects of warming middle-jiao and descending qi, benefiting intestines and stomach, stopping hiccup, tonifying kidney and replenishing primordial qi and the like. In modern medical research, there is increasing evidence that bioactive peptide components in plant extracts can positively affect human health through a variety of mechanisms. The white kidney bean extract can specifically inhibit the activity of alpha-amylase in human body, retard the hydrolysis and digestion of starch in food, effectively reduce postprandial blood glucose level and reduce energy intake. Therefore, white kidney beans can be selected to develop the white kidney bean polypeptide with stable and simple preparation technology and good blood sugar reducing effect, so that a new raw material is provided for preventing and assisting in controlling the blood sugar level of diabetics, and a new benefit growth point is also found for the white kidney bean industry. Disclosure of Invention In view of the deficiencies of the prior art, a primary object of the present invention is to provide a white kidney bean polypeptide having an amino acid sequence of Asp-Asn-Pro-Phe-Tyr-Phe (DNPFYF). The second object of the present invention is to provide a method for producing the white kidney bean polypeptide, which is produced from white kidney beans hydrolyzed by alkaline protease (serine protease), comprising the following steps: (1) Air drying semen lablab album, pulverizing, sieving, collecting sieved bean powder, adding water into the bean powder, and ultrasonic extracting. (2) And (3) regulating the solution obtained in the step (1) to be alkaline, then adding alkaline protease for enzymolysis to obtain an enzymolysis solution, filtering the enzymolysis solution with a filter bag, collecting filtrate, and freeze-drying the filtrate to obtain a dried product. (3) Redissolving the dried substance, separating with 10kDa ultrafiltration membrane, and collecting components with molecular weight less than 10 kDa. (4) The polypeptide with the amino acid sequence DNPFYF can be obtained by subjecting the component with the molecular weight less than 10kDa to liquid chromatography-mass spectrometer. Preferably, the pore size of the sieve in step (1) is 0.84mm. Preferably, the solid-to-liquid ratio of the soybean powder to the water in the step (1) is 1:10, and the unit is kg/L. Preferably, the alkaline protease is added in the step (2) in an amount of 1.1% by mass of the solution. Preferably, the enzymolysis condition in the step (2) is 50 ℃ for 3 hours. Preferably, the filter bag used in step (2) is 200 mesh. The invention further aims to provide an application of Asp-Asn-Pro-Phe-Tyr-Phe white kidney bean polypeptide in preparation of hypoglycemic drugs. The fourth object of the invention is to provide an application of Asp-Asn-Pro-Phe-Tyr-Phe white kidney bean polypeptide in preparing health care products which are helpful for maintaining blood sugar health level. Compared with the prior art, the invention has the following beneficial effects: (1) The average value of the inhibition rate of the polypeptide DNPFYF to alpha-amylase at 0.1, 0.2, 0.4, 0.6 and 1mg/ml is 69.24%, 89.68%, 94.86%, 96.02% and 97.65%, the average value of the inhibition rate to alpha-glucosidase is 7.33%, 12.70%, 58.38%, 72.63% and 98.61%, the water solubility is good, the average value retention time in human body is long, the acute oral toxicity is small, and the polypeptide DNPFYF can be used for preparing hypoglycemic drugs. (2) The polypeptide can be obtained not only by an artificial synthesis mode, but also by being prepared from white kidney beans, and a new way is provided for the utilization of the white kidney beans. Dr