CN-122011108-A - Buthus martensii Karsch polypeptide with EV71 virus-resistant activity and application thereof

Abstract

The invention discloses an east Asia scorpion polypeptide with EV71 virus-resistant activity and application thereof, belonging to the technical field of traditional Chinese medicine polypeptides. The Buthus martensii Karsch polypeptide with EV71 virus resisting activity is Pep 15 or Pep16, the amino acid sequence of Pep 15 is shown in SEQ ID No.1, and the amino acid sequence of Pep16 is shown in SEQ ID No. 2. The Buthus martensii Karsch polypeptide has obvious EV71 resisting activity, can inhibit the cytopathic effect of EV71 virus on host cell Vero, increase the survival rate of infected cell, inhibit the replication and proliferation of EV71 virus inside cell, has no toxic effect on cell when the concentration of Buthus martensii Karsch polypeptide reaches 200 mu M, has the potential of being used as EV71 resisting medicine, and has excellent application foreground in developing new medicine for preventing and treating infant hand-foot-mouth disease induced by EV71 virus.

Inventors

• CAO PENG
• YANG QING
• GENG ZHIRONG
• HOU LIJING
• CHEN XINGYU
• SUN TIEFENG

Assignees

• 南京中医药大学
• 山东省中医药研究院

Dates

Publication Date

20260512

Application Date

20260413

Claims (3)

1. 1. The Buthus martensii Karsch polypeptide with EV71 virus resisting activity is characterized in that the Buthus martensii Karsch polypeptide with EV71 virus resisting activity is Pep 15 or Pep 16; The amino acid sequence of Pep 15 is shown as SEQ ID NO. 1; the amino acid sequence of Pep 16 is shown as SEQ ID NO. 2.
2. 2. The Buthus martensii Karsch polypeptide with EV 71-resisting virus activity according to claim 1, wherein the Buthus martensii Karsch polypeptide with EV 71-resisting virus activity is synthesized by a solid-phase synthesis method.
3. 3. Use of the east asian scorpion polypeptide having anti-EV 71 virus activity of claim 1 or 2 in the preparation of anti-EV 71 virus medicament.

Description

Buthus martensii Karsch polypeptide with EV71 virus-resistant activity and application thereof Technical Field The invention belongs to the technical field of traditional Chinese medicine polypeptides, and particularly relates to an east Asia scorpion polypeptide with EV71 virus-resistant activity and application thereof. Background Hand-foot-and-mouth disease (and mouth disease, HFMD) is a global infectious disease caused by various enteroviruses, and mainly affects children under 5 years old, and has become an important public health challenge. Some severe cases can involve the nervous system, circulatory system and respiratory system, which can pose a serious threat to the life of the infant. Enterovirus type 71 (Enterovirus, EV71) belongs to the genus enterovirus (Enterovirus) of the family picornaviridae (Picornaviridae), and as a representative of non-enveloped RNA viruses, its viral particles comprise icosahedral capsids composed of VP1, VP2 and VP3 proteins, internally enveloped by a sense RNA strand about 7.5 kb long, is the main causative agent of HFMD. The EV71 virus infection has the clinical characteristics of high infectivity and rapid progress, has obvious neurotropic property, is easy to induce severe infection, and can be complicated with brainstem encephalitis, aseptic meningitis, pulmonary complications and the like in serious patients, and the death rate is about 3.1 percent. At present, no specific anti-EV 71 virus medicine exists clinically, and interferon, ribavirin, methylprednisolone and the like are combined for administration, but the curative effect on severe HFMD caused by EV71 viruses is limited. Therefore, the development of novel specific anti-EV 71 drugs has important significance for preventing and treating HFMD. The dried whole Buthus martensii Karsch (Buthus MARTENSII KARSCH) is the basic source medicine of the traditional animal medicine Buthus martensii Karsch, which is recorded in Chinese pharmacopoeia of 2025 edition, and has the effects of calming endogenous wind and relieving spasm, eliminating toxin and resolving hard mass, and dredging collaterals and relieving pain. Recent studies have shown that toxin polypeptides derived from scorpion species have inhibitory activity against a variety of viruses, such as influenza virus, dengue virus, hepatitis c virus, herpes simplex virus, and human immunodeficiency virus. Studies show that antibacterial peptide BmKn derived from Buthus martensii Karsch and its derivative BmKn2-T5 can inhibit EV71 virus replication obviously in vitro, the inhibition mainly occurs in early stage of virus life cycle, and antibacterial peptides derived from Scorpio with different structures or properties show different pharmacological activities in EV71 virus infection. No report on screening, identifying and preparing single active polypeptide compounds based on the Tokyo scorpion polypeptide histology strategy and systematically evaluating the anti-EV 71 virus activity is found in the prior art. Disclosure of Invention Aiming at the problems in the prior art, the invention provides the Buthus martensii Karsch polypeptide with EV71 virus-resisting activity and the application thereof, and the Buthus martensii Karsch polypeptide has good EV71 virus replication inhibiting effect and provides theoretical basis for biomedical research and clinical application. The invention is realized by the following technical scheme: In a first aspect of the present invention, an east asian scorpion polypeptide having anti-EV 71 virus activity is provided, wherein the east asian scorpion polypeptide having anti-EV 71 virus activity is Pep 15 or Pep 16; The amino acid sequence of Pep 15 is shown as SEQ ID NO. 1; the amino acid sequence of Pep 16 is shown as SEQ ID NO. 2. Further, the Buthus martensii Karsch polypeptide with EV71 virus-resistant activity is synthesized by a solid-phase synthesis method. In a second aspect, the invention provides an application of the east Asia scorpion polypeptide with anti-EV 71 virus activity in preparing anti-EV 71 virus medicaments. Compared with the prior art, the invention has the following beneficial effects: The Buthus martensii Karsch polypeptide (Pep 15 or Pep 16) has obvious EV71 resisting activity, inhibits cytopathic effect (CPE) of EV71 virus on host cell Vero, increases the survival rate of infected cells, inhibits the replication and proliferation of EV71 virus in cells, has no toxic effect on cells when the concentration of the Buthus martensii Karsch polypeptide reaches 200 mu M, has potential as an EV71 resisting medicine, and has good application prospect in the aspect of developing novel medicines for preventing and treating infant hand-foot-mouth disease induced by EV71 virus. Drawings FIG. 1 is a graph showing the results of the inhibition of EV71 virus by different Buthus martensii Karsch polypeptides; FIG. 2 is an HPLC and MS spectra of the Buthus martensii Karsch polypeptide Pep 15, (a) HPLC and (b) MS; F