CN-122011110-A - Undaria pinnatifida sporophyll source active peptide and preparation method and application thereof

Abstract

The invention belongs to the technical field of biology, and discloses a undaria pinnatifida sporophyll source active peptide, and a preparation method and application thereof. The undaria pinnatifida sporophyll source active peptide is at least one peptide with an amino acid sequence shown as SEQ.ID.NO. 1-4. The undaria pinnatifida sporophyll source active peptide is obtained by screening undaria pinnatifida sporophylls through a bioinformatics tool and molecular docking. Experiments prove that the undaria pinnatifida sporophyll source active peptide has antioxidant and anti-inflammatory activities, and can be used for preparing antioxidant or antioxidant-assisted products and anti-inflammatory products.

Inventors

• YU HUI
• YANG GANGQIANG
• TAN LIMAN
• ZHENG YI
• Hao Ziqian
• LIU HAIMEI
• ZHAO QIN

Assignees

• 鲁东大学
• 烟台大学

Dates

Publication Date

20260512

Application Date

20260414

Claims (9)

1. 1. The sporophyll source active peptide of undaria pinnatifida is characterized by being at least one peptide with an amino acid sequence shown as SEQ.ID.NO. 1-4.
2. 2. The method for preparing the undaria pinnatifida sporophyll-derived active peptide according to claim 1, which comprises the following steps: S1, obtaining sporophyll peptide of undaria pinnatifida; S2, carrying out sequence identification on the undaria pinnatifida sporophyll peptide; s3, molecular docking is carried out on the undaria pinnatifida sporophyll peptide and a receptor Keap1, and the peptide is screened.
3. 3. The method according to claim 2, wherein in step S1, the Undaria pinnatifida sporophyll peptide is obtained by subjecting a Undaria pinnatifida sporophyll protein material to enzymolysis.
4. 4. The method according to claim 3, wherein in step S1, the raw material of the sporophyll protein of undaria pinnatifida is subjected to enzymolysis by using neutral protease.
5. 5. The method of claim 2, wherein the potential biological activity of the peptide is predicted in step S3 using PEPTIDERANKER tools prior to molecular docking.
6. 6. The method of claim 5, wherein the peptide is selected to have a bioactivity score of > 0.5.
7. 7. The method according to claim 2, wherein in step S3: Prior to molecular docking, the physical properties of the peptides were predicted using the Innovagen tool; prior to molecular docking, stability of the peptide was predicted using the ProtParam tool; prior to molecular docking, the solubility of the peptide was predicted using admetSAR tools; Prior to molecular docking, the use of AllergenOnline tools to predict peptide sensitization; prior to molecular docking, toxinPred tools were used to predict potential toxicity of the peptides.
8. 8. Use of an active peptide of undaria pinnatifida sporophyll origin according to claim 1 in or in the preparation of an antioxidant product.
9. 9. Use of an active peptide of undaria pinnatifida sporophyll origin according to claim 1 in the preparation of an anti-inflammatory product.

Description

Undaria pinnatifida sporophyll source active peptide and preparation method and application thereof Technical Field The invention belongs to the technical field of biology, and particularly relates to a undaria pinnatifida sporophyll source active peptide, and a preparation method and application thereof. Background The antioxidant peptide is a bioactive peptide with antioxidant activity, has the characteristics of easy absorption, good stability, no immunogenicity and the like, and has multiple physiological functions of nutrition enhancement, immune regulation and the like. The antioxidant peptide is widely applied in the food industry because of natural sources, high safety, no toxic or side effect and outstanding nutritional value, and has become one of natural active ingredients which are paid attention to the field of functional foods. Therefore, development of antioxidant peptides with both nutrition and safety by using low-cost biological resources has been an important direction in the field of polypeptide research. Anti-inflammatory peptides are a class of small molecule peptides with anti-inflammatory activity that can effectively alleviate related symptoms by modulating the inflammatory response. It has the advantages of easy absorption, high stability, no immunogenicity, and the like, and has nutritive value and multiple bioactivity functions, such as immunoregulation, tissue repair promotion, and the like. The natural anti-inflammatory peptide has wide application prospect in the fields of medicines, cosmetics, foods and the like because of the advantages of natural, safety, non-toxicity, high nutritive value and the like, and becomes one of important natural products in the fields of functional foods and biological medicines. Therefore, the development of anti-inflammatory peptides with high anti-inflammatory activity and good safety by using low-cost biological resources is always a research hotspot in the field of polypeptides. Oxidative stress and inflammatory response have a close correlation and mechanism of action in the living body. On the one hand, excessive reactive oxygen species activate multiple cell signaling pathways (e.g., NF- κb) that promote pro-inflammatory factor expression, initiate or exacerbate inflammatory responses, and on the other hand, sustained inflammatory conditions can also lead to immune cells producing significant amounts of reactive oxygen species, forming a vicious circle. The Keap1-Nrf2 pathway is a core regulation pivot of cell resistance to oxidative stress and inflammatory reaction, so that the development of antioxidant and anti-inflammatory polypeptides with synergistic effect through targeted activation of the Nrf2 pathway has become an important strategy in the fields of functional foods, special medical foods, biological medicines and the like, and the application potential of the polypeptide is superior to that of single active polypeptide. The undaria pinnatifida is an important large-scale economic brown algae, and is rich in various nutrients and bioactive components. The sporophylls, which are the reproductive organs, are valuable biological resources, although the fiber content is high and the palatability is poor. At present, after sporophyll polysaccharide is extracted by main seaweed processing enterprises such as Shandong and Liaoning, the obtained byproducts still have high protein content and are not effectively utilized. However, the research on the deep enzymatic conversion of protein resources in such byproducts is not sufficient, and particularly in the aspect of systematically preparing, isolating and identifying polypeptides with definite biological activities, efficient high-value development has not been achieved yet. The virtual screening of the active peptide based on the bioinformatics tool and the molecular docking technology refers to the simulation prediction of the potential bioactivity, safety and structure-activity relationship of the active peptide by means of related databases and professional software according to the amino acid sequence of the known active peptide, so as to determine the target peptide fragment. The method further realizes rapid and accurate targeted screening of the target active peptide by evaluating the interaction between the ligand and the receptor. Compared with the traditional preparation method, the time and experimental cost can be obviously saved by utilizing bioinformatics and molecular docking for virtual screening. In actual operation, the screening efficiency and the result reliability can be effectively improved by combining the two. The strategy is adopted to screen bioactive peptide from the sporophyll byproducts of undaria pinnatifida, and provides important technical support for developing products with auxiliary antioxidant and anti-inflammatory functions. Disclosure of Invention Aiming at the defects of the prior art, the invention provides a undaria pinnatifida sporophyll source