CN-122011121-A - Polypeptide and probe for targeting elastic fiber and application of polypeptide and probe

Abstract

The invention discloses a polypeptide targeting elastic fiber, a probe and application thereof, wherein the amino acid sequence of the polypeptide is YGQVPMCDAGEQCAVRKGARI, the elastic fiber can be specifically identified, and the polypeptide has good biocompatibility and can be used as a tool for living body imaging, drug delivery, function regulation and the like. Since the sequences are identical in humans and rodents, they can be used directly in rodent models for fundamental and preclinical studies related to elastin.

Inventors

• ZHANG HE

Assignees

• 大连医科大学

Dates

Publication Date

20260512

Application Date

20251231

Claims (7)

1. 1. A polypeptide targeting elastic fiber is characterized in that the amino acid sequence of the polypeptide is YGQVPMCDAGEQCAVRKGARI.
2. 2. A fiber-targeting probe, characterized in that it is a probe prepared with the fiber-targeting polypeptide of claim 1.
3. 3. The elastic fiber targeting probe according to claim 2, characterized by being a fluorescent probe, a radioactive probe or a magnetic resonance imaging probe.
4. 4. A fiber-targeted probe according to claim 3, characterized by being a fluorescent probe formed by coupling a fiber-targeted polypeptide with a Cy5 fluorescent dye.
5. 5. The elastic fiber targeting probe according to claim 4, wherein the elastic fiber targeting polypeptide and the Cy5 fluorescent dye are coupled in a click chemistry reaction mode.
6. 6. Use of a probe according to claim 2,3, 4 or 5 for targeting elastic fibres for in vivo or in vitro localization, imaging or detection of elastic fibres.
7. 7. Use of the elastic fiber-targeting polypeptide of claim 1 for basic research, disease diagnosis, targeted drug delivery or functional regulation related to elastic fiber.

Description

Polypeptide and probe for targeting elastic fiber and application of polypeptide and probe Technical Field The invention belongs to the field of medical cell biology, and particularly relates to a polypeptide and a probe for targeting elastic fibers and application thereof. Background Elastic fibers are important components of connective tissue and are widely distributed in tissues such as skin, lung, blood vessels and the like, so that the tissues have elasticity. The fiber mainly comprises elastin, microfiber and other components, and the stability and biological activity of the configuration of the fiber are critical to the normal operation of tissues. However, under various pathological conditions such as aging, ultraviolet radiation, persistent inflammation and the like, related elastic fibers are often damaged, decomposed or abnormally remodeled, so that the tissue elasticity is obviously weakened, and then abnormal functions are caused. If the device can be used for accurate positioning detection or intervention regulation, the device has basic research value and medical diagnosis and treatment value. The existing method for identifying the elastic fiber mainly comprises elastic fiber dye dyeing, antibody marking and the like, and can be used for imaging the elastic fiber in a tissue section, but has the problems of poor penetrability, limited specificity, inapplicability to living body imaging and the like. The invention patent application with the Chinese patent application number 202310408541.7 discloses a novel targeted elastin-binding probe and biomedical application thereof, wherein the probe is a fluorescent probe based on glycopeptide antibiotics or lipopeptide antibiotics, and mainly comprises fluorescent probes prepared on the basis of vancomycin, teicoplanin, telavancin and the like, wherein the antibiotic component is a microbial metabolite, and the application of the fluorescent probe to living bodies can cause side effects such as kidney injury, allergy and the like, and the clinical application needs to be strictly controlled. The targeting polypeptide can realize accurate positioning of specific molecules by specifically recognizing and combining target cells/target molecules, and has the advantages of high specificity, easiness in modification and the like. The action mode is as follows: 1) Direct modulation, which can be used as an active molecule by itself, directly inhibiting/activating a signal pathway by binding to a target; 2) The molecular marker is combined with a fluorescent group, a radionuclide and the like to prepare a probe, and the probe specifically recognizes pathological tissue cells, so that early and accurate diagnosis of diseases, such as image detection, pathological biopsy and the like, is realized. 3) Drug delivery, namely, drug is loaded and accurately delivered to a lesion part, so that damage to normal cells is reduced, and toxic and side effects of the drug on an organism are reduced; however, no specific polypeptide, probe and application report targeting elastic fiber has been found so far. Disclosure of Invention The invention aims to solve the technical problems in the prior art and provides a polypeptide and a probe for targeting elastic fibers and application thereof. The technical scheme of the invention is that the polypeptide targeting the elastic fiber has an amino acid sequence YGQVPMCDAGEQCAVRKGARI. The elastic fiber targeting probe is prepared with the elastic fiber targeting polypeptide. Preferred probes are fluorescent probes, radioactive probes or magnetic resonance imaging probes. Preferred probes are fluorescent probes formed by coupling a polypeptide targeting an elastic fiber with a Cy5 fluorescent dye. The preferred probe is a conjugate of a polypeptide targeting an elastic fiber with a Cy5 fluorescent dye by click chemistry. The use of a probe as described above for targeting elastic fibres for in vivo or in vitro localization, imaging or detection of elastic fibres. Use of a polypeptide as described above that targets elastic fibers in basic research, disease diagnosis, targeted drug delivery or functional regulation associated with elastic fibers. The polypeptide of the targeted elastic fiber provided by the invention can specifically identify the elastic fiber, has good biocompatibility, and can be used as a tool for living body imaging, drug delivery, function regulation and the like. Since the sequences are identical in humans and rodents, they can be used directly in rodent models for fundamental and preclinical studies related to elastin. Drawings FIG. 1 is a graph of detection of a mouse skin elastic fiber targeting signal in an embodiment of the invention. Detailed Description Example 1 polypeptide screening and preparation of Targeted elastic fibers The amino acid sequence of the polypeptide targeting elastic fiber is YGQVPMCDAGEQCAVRKGARI, and the polypeptide targeting elastic fiber is obtained based on human CARTPT gene scr