CN-122011133-A - Novel antibacterial peptide wrGVC-AMP-S7 from wild rodent intestinal phage and application thereof

Abstract

The invention discloses a novel antibacterial peptide wrGVC-AMP-S7 derived from wild rodent intestinal phage and application thereof, belonging to the technical field of biology. The MIC measurement result shows that the antibacterial peptide wrGVC-AMP_S7 has antibacterial activity to various gram-positive bacteria and gram-negative bacteria with different degrees. At the highest test concentration of 128 mug/mL, the hemolysis rate is still lower than 10%, and the low hemolytic activity and good biosafety are shown. The cytotoxicity test results show that the antibacterial peptide AMP_S7 does not show obvious cytotoxicity to IEC-6 cells. The cell viability remained above 95% even at high concentration of 128 μg/mL, indicating that the antimicrobial peptide has good cell compatibility.

Inventors

• ZHANG XIAOXUAN
• NI HONGBO
• MA HE
• LIU RUI
• Nan Fulong
• YU HAILONG

Assignees

• 青岛农业大学

Dates

Publication Date

20260512

Application Date

20260414

Claims (4)

1. 1. A novel antibacterial peptide wrGVC-AMP-S7 from wild rodent intestinal phage is characterized in that the amino acid sequence is shown in SEQ ID NO. 1.
2. 2. The use of the novel antibacterial peptide wrGVC-AMP-S7 of claim 1 in the preparation of a broad-spectrum antibacterial formulation.
3. 3. The use according to claim 2, wherein the broad-spectrum antibacterial formulation has an inhibitory effect on the following bacteria: Riemerella anatipestifer, mannheimia, proteus mirabilis, staphylococcus haemolyticus, pasteurella, clostridium perfringens, pseudomonas aeruginosa, salmonella, staphylococcus aureus, escherichia coli, streptococcus suis type II, enterococcus faecalis, and Klebsiella pneumoniae.
4. 4. The use according to claim 2 or 3, wherein the concentration of the novel antibacterial peptide wrGVC-AMP-S7 is 16-128 μg/mL.

Description

Novel antibacterial peptide wrGVC-AMP-S7 from wild rodent intestinal phage and application thereof Technical Field The invention belongs to the technical field of biology, and particularly relates to a novel antibacterial peptide wrGVC-AMP-S7 derived from wild rodent intestinal phage and application thereof. Background In the modern animal husbandry production process, antibiotics are widely used for preventing and controlling animal epidemic diseases and regulating production performance for a long time. However, the continuous use of antibiotics, while effectively controlling bacterial infections, accelerates the generation and spread of drug-resistant pathogenic bacteria, bringing potential risks to animal health, food safety, and public health. With the increasing prominence of antibiotic resistance, a number of countries and regions have been subject to successive related policies that severely limit the use of antibiotics in animal husbandry. Under the background, the search for safe and effective antibiotic substitutes has become an important research direction for promoting the sustainable development of animal husbandry. The antibacterial peptide is a small molecular active peptide widely existing in the nature, generally has the characteristics of high action speed, multiple action mechanisms and the like, and has good application potential in the aspect of inhibiting or killing pathogenic bacteria. In recent years, the research of antibacterial peptides as novel anti-infective molecules has been continuously focused, and is considered to have important value in prevention and control of diseases in animal husbandry and antibiotic substitution applications. To further expand the variety and source of antimicrobial peptides, the continued development of new antimicrobial peptide resources has become an important research in this field. With the rapid development of metagenome sequencing technology, researchers can systematically analyze genetic information in complex microbial communities under the condition of no need of separating bacteria, and a new technical means is provided for high-throughput excavation of functional molecules. Meanwhile, the artificial intelligence method such as deep learning has remarkable advantages in the aspects of biological sequence pattern recognition and function prediction, and can be used for efficiently screening candidate peptide sequences with potential antibacterial activity from massive sequence data. The novel antibacterial peptide is mined based on metagenome data and combined with a deep learning model, and the novel antibacterial peptide has become an important development direction and research hotspot in the current antibacterial peptide research field. Wild rodents are widely distributed in diverse natural ecological environments, intestinal microbiota of the rodents are formed under the action of long-term evolution and complex environmental factors, and the rodents have remarkable diversity and uniqueness. The intestinal phage is used as an important component of a microbial community, plays an important role in regulating the structure of the microbial community and the microecological balance, and can contain antibacterial related functional molecules with potential application value in the genome. However, the current systematic screening for wild rodent intestinal phage-derived antibacterial peptides and their functional and application studies are still relatively limited, and related resources are still yet to be further developed. Therefore, the antibacterial peptide with broad-spectrum antibacterial activity is mined and identified from wild rodent intestinal phage resources, and has important significance for enriching antibacterial peptide sources and promoting the development of antibiotic substitution technology. Disclosure of Invention In view of the above, the present invention aims to provide a novel antibacterial peptide wrGVC-AMP-S7 derived from wild rodent intestinal phage and use thereof, wherein the novel antibacterial peptide wrGVC-AMP-S7 has broad-spectrum antibacterial property and high safety. In order to achieve the above object, the present invention provides the following technical solutions: The invention provides a novel antibacterial peptide wrGVC-AMP-S7 from wild rodent intestinal phage, the amino acid sequence of which is shown as SEQ ID NO. 1. The invention provides application of the novel antibacterial peptide wrGVC-AMP-S7 in preparation of a broad-spectrum antibacterial preparation. Further, the broad-spectrum antibacterial formulation has an inhibitory effect on the following bacteria: Riemerella anatipestifer, mannheimia, proteus mirabilis, staphylococcus haemolyticus, pasteurella, clostridium perfringens, pseudomonas aeruginosa, salmonella, staphylococcus aureus, escherichia coli, streptococcus suis type II, enterococcus faecalis, and Klebsiella pneumoniae. Further, the concentration of the novel antibacterial