CN-122011134-A - Banana streak virus vaccine and application thereof in preventing and treating banana streak virus disease

Abstract

The invention belongs to the technical field of bioengineering, and particularly relates to a banana streak virus vaccine and application thereof in preventing and treating banana streak virus diseases. According to the invention, research and exploration are carried out, and the banana streak virus vaccine constructed based on the endogenous banana streak virus sequence carried by the banana genome is developed, so that after the banana streak virus vaccine is applied to banana plants, the banana streak virus replication can be obviously inhibited, and the banana streak virus infection and the banana streak virus disease development can be controlled. The invention provides a novel strategy for simply, conveniently and effectively preventing and treating banana streak virus diseases, which is particularly suitable for large-scale banana planting areas.

Inventors

• RAO XUEQIN
• LIU RUNPEI
• Pu shuang
• Mao Zixian

Assignees

• 华南农业大学

Dates

Publication Date

20260512

Application Date

20251218

Claims (10)

1. 1. The application of endogenous banana streak virus sequences BEV UC and/or BEV GZ5 in the preparation of products for preventing and controlling banana streak virus or preventing banana streak virus diseases is provided, wherein the sequence of BEV UC is shown as SEQ ID NO.1, and the sequence of BEV GZ5 is shown as SEQ ID NO. 2.
2. 2. The recombinant plasmid is characterized by comprising endogenous banana streak virus sequences BEV UC and/or BEV GZ5, wherein the sequence of the BEV UC is shown as SEQ ID NO. 1, and the sequence of the BEV GZ5 is shown as SEQ ID NO. 2.
3. 3. The recombinant plasmid according to claim 2, wherein the backbone of the recombinant plasmid is selected from any one of pGD vector, pPZP3425 vector, pJLTRBO vector.
4. 4. A recombinant engineering bacterium comprising the recombinant plasmid of claim 2 or 3.
5. 5. Use of the recombinant plasmid according to claim 2 or 3 or the recombinant engineering bacterium according to claim 4 for preparing a product for preventing and controlling banana streak virus or preventing and controlling banana streak virus diseases.
6. 6. A banana streak virus vaccine comprising the recombinant plasmid of claim 2 or 3 or the recombinant engineering bacterium of claim 4.
7. 7. A method for controlling banana streak virus or preventing banana streak virus disease, characterized in that banana plants are treated with the vaccine of claim 6.
8. 8. The method for preparing recombinant engineering bacteria according to claim 4, wherein the endogenous banana streak virus sequences BEV UC and/or BEV GZ5 are connected to a plant expression vector to obtain recombinant plasmids, and the recombinant plasmids are transformed into agrobacterium strains to obtain the recombinant engineering bacteria for expressing the endogenous banana streak virus sequences BEV UC and/or BEV GZ 5.
9. 9. The method according to claim 8, wherein the plant expression vector is selected from the group consisting of pGD vector, pPZP3425 vector, pJLTRBO vector, and the Agrobacterium is selected from the group consisting of Agrobacterium EHA105, GV3101, LBA 4404.
10. 10. The method of preparing as claimed in claim 8, comprising the steps of: s1, extracting total DNA of bananas; S2, performing PCR amplification on the DNA obtained in the S1 by using a primer pGD-UC-F/pGD-UC-R or a primer pGD-GZ5-F/pGD-GZ5-R to obtain an endogenous banana streak virus sequence BEV UC or BEV GZ5; the sequence of the primer pGD-UC-F/pGD-UC-R is shown as SEQ ID NO 3-4; The sequence of the primer pGD-GZ5-F/pGD-GZ5-R is shown as SEQ ID NO. 5-6; s3, connecting the endogenous banana streak virus sequence BEV UC or BEV GZ5 obtained in the S2 to a pGD vector to obtain a recombinant plasmid pGD UC or pGD GZ5; s4, converting the recombinant plasmid pGD UC or pGD GZ5 obtained in the S3 into an agrobacterium strain to obtain recombinant engineering bacteria.

Description

Banana streak virus vaccine and application thereof in preventing and treating banana streak virus disease Technical Field The invention belongs to the technical field of bioengineering. More particularly, it relates to banana streak virus vaccines and their use in the prevention and treatment of banana streak virus diseases. Background Banana streak virus (banana streak virus, BSV) belongs to the genus of baculo DNA virus of the family Cauliflower mosaic virus, is a main pathogen causing banana streak virus diseases, is widely distributed in each large banana producing area in China, and typical infection symptoms of the banana streak virus are marked by fading stripes, mottle or necrotic spots on leaves, and plant dwarfing, slow growth, cluster malformation and even sterilization are caused when the infection is serious, so that the yield and commodity value are obviously reduced, and the banana streak virus is one of important pathogens restricting sustainable development of banana industry. BSV has different propagation ways, namely long-distance propagation through toxic asexual propagation materials (such as bud sucking and tissue culture seedling) and field diffusion through mealybugs as mediators. Further complicating matters, BSVs also possess unique "endogenous" characteristics, and a portion of the viral sequences can integrate into the banana host genome to form endogenous banana streak virus (endogenous Banana streak virus, eBSV). Under certain conditions of environmental stress (e.g., high temperature, drought) or in vitro tissue culture, these silent viral sequences may be activated, recombined and released as infectious whole virus particles. The mechanism not only makes the BSV difficult to eradicate, but also greatly increases the difficulty of seedling health detection. At present, no effective prevention and control means exists for BSV, and the prevention and control strategy still mainly depends on source control, including strict screening and cultivation of virus-free seedlings, establishment of standardized detoxification and detection processes, enhancement of field monitoring and timely isolation or eradication of definite disease plants. Therefore, the search for new means for controlling BSV is a major challenge in this field. Banana endogenous viral sequences (banana endogenous virus sequence, BEVs) have been found in banana genomes in recent years (GEERINGET al., 2005), these BEVs contain partial reverse transcriptase (REVERSE TRANSCRIPTASE, RT) and ribonuclease H (RNase H) gene nucleotide sequences, are widely distributed in banana a genome (Musa acuminata) and banana B genome (m. balbisiana), and have become banana genetic material (Bhatet al., 2016). Among the BEVs currently known are BEV UD, BEV UH, BEV NGA, BEV UF, BEV P, BEV Q, BEV UC, BEV UG, etc., among which BEVs BEV UC is most widely distributed in the banana genome. Recently, studies have found a new BEV GZ5 (Chenet al, 2024) containing the complete RT/RNase H in the genome of plantain et al. Although there are a large number of BEVs in the banana genome, the role of these BEVs in BSV-infected bananas is currently unclear. Disclosure of Invention Aiming at the problem that no effective prevention and control means are available for banana streak viruses in the prior art, the invention aims at exploring the relation between Banana Endogenous Viral Sequences (BEVs) and banana streak viruses on banana infection, develops banana streak virus vaccines based on the BEVs, and provides a new efficient prevention and control strategy for banana streak viruses. A first object of the present invention is to provide the use of the endogenous banana streak virus sequences BEV UC and/or BEV GZ5 in the preparation of a product for controlling banana streak virus or preventing banana streak virus diseases. A second object of the present invention is to provide a recombinant plasmid. The third object of the present invention is to provide a recombinant engineering bacterium. The fourth object of the invention is to provide the application of the recombinant plasmid or the recombinant engineering bacteria in preparing products for preventing and controlling banana streak virus or preventing and controlling banana streak virus diseases. A fifth object of the invention is to provide a banana streak virus vaccine. A sixth object of the present invention is to provide a method for controlling banana streak virus or preventing banana streak virus disease. The seventh object of the invention is to provide a preparation method of the recombinant engineering bacteria. The above object of the present invention is achieved by the following technical scheme: The invention discloses application of an endogenous banana streak virus sequence BEV UC and/or BEV GZ5 in preparation of a product for preventing and controlling banana streak virus or preventing banana streak virus diseases, wherein the BEV UC has a sequence shown as SEQ ID NO. 1, and the