CN-122011145-A - SNR protein mutant and encoding gene and application thereof

Abstract

The invention provides an SNR protein mutant, and a coding gene and application thereof, and belongs to the technical field of plant genetic engineering. According to the invention, three different target sequences are selected on the SNR genes of the rice, three different SNR gene mutants are obtained after gene knockout, and after the three SNR mutant genes are transferred into the rice, the rice plant height reducing effect is mild, the fertility of filial generation is good, and other agronomic characters such as thousand seed weight are not obviously changed.

Inventors

• CHENG XIAOYAN
• ZOU GUOXING
• HUANG YONGPING

Assignees

• 江西省农业科学院水稻研究所

Dates

Publication Date

20260512

Application Date

20251222

Claims (10)

1. 1. The SNR protein mutant is characterized in that the amino acid sequence of the SNR protein mutant is any one of SEQ ID No.21, SEQ ID No.23 and SEQ ID No.25.
2. 2. A nucleic acid molecule encoding the SNR protein mutant of claim 1.
3. 3. The nucleic acid molecule of claim 2, wherein the nucleotide sequence encoding the protein mutant of SEQ ID No.21 is shown in SEQ ID No.20, the nucleotide sequence encoding the protein mutant of SEQ ID No.23 is shown in SEQ ID No.22, and the nucleotide sequence encoding the protein mutant of SEQ ID No.25 is shown in SEQ ID No. 24.
4. 4. A recombinant expression vector comprising the nucleic acid molecule of claim 2.
5. 5. A genetically engineered bacterium comprising the recombinant expression vector of claim 4, or the genome of the genetically engineered bacterium comprising the nucleic acid molecule of claim 2.
6. 6. The genetically engineered bacterium of claim 5, wherein the host of the genetically engineered bacterium is agrobacterium EHA105.
7. 7. The use of the SNR protein mutant of claim 1, the nucleic acid molecule of claim 2 or 3, the recombinant expression vector of claim 4, the genetically engineered bacterium of claim 5 or 6 for reducing the plant height of rice.
8. 8. The use of the SNR protein mutant of claim 1, the nucleic acid molecule of claim 2 or 3, the recombinant expression vector of claim 4, the genetically engineered bacterium of claim 5 or 6 for breeding semi-dwarf rice varieties with passage function.
9. 9. A method for cultivating semi-dwarf rice, which is characterized by comprising at least one of the following steps: (1) Performing gene editing on target rice to ensure that the gene of the target rice codes for the SNR protein mutant of claim 1; (2) Introducing the nucleic acid molecule of claim 2 or 3 into a cell of a rice of interest; (3) Transforming the recombinant expression vector of claim 4 into a rice plant of interest; (4) Transforming the genetically engineered bacterium of claim 5 or 6 into a target rice.
10. 10. The cultivation method according to claim 9, wherein any one of the methods (1) to (4) comprises the steps of selecting three different target sequences from rice SNR genes, knocking out the rice SNR genes by using CRISPR/Cas as a gene editing tool, wherein the three different target sequences are respectively shown as SEQ ID No.5, SEQ ID No.6 and SEQ ID No. 7.

Description

SNR protein mutant and encoding gene and application thereof Technical Field The invention belongs to the technical field of plant genetic engineering, and particularly relates to an SNR protein mutant, a coding gene of the SNR protein mutant and application thereof. Background The global population continues to grow and the demand for food continues to rise. The rice is taken as one of the main grain crops in the world, is staple food for about 35 hundred million people worldwide, can directly increase the total yield of grains by improving unit yield, relieves the pressure of grain shortage, and avoids famine or social agitation caused by insufficient grain supply. Especially for countries (such as China, india, japan, etc.) taking rice as main food, improving rice yield per unit can reduce dependence on imported grain, and ensure national grain safety. In recent years, with the progress of urban and industrialized cultivation, a large amount of cultivated land is occupied, and the global cultivated land area tends to be reduced year by year. The rice yield per unit is improved, more grains can be produced on the limited cultivated land area, ecological fragile areas such as forests, wetlands and the like are prevented from being excessively reclaimed for enlarging the cultivated area, and the biodiversity and the ecological environment can be protected. Plant height is one of the important factors in determining crop yield. Too high plants often result in too thin stalks and reduced load bearing capacity, which in turn leads to plants that are prone to lodging. Lodging (stalk breakage or inclination) of rice can cause photosynthesis to be blocked, influence nutrient transportation and grain grouting, inhibit root system development, and finally cause unit yield reduction. Lodging also aggravates plant diseases and insect pests, further damages the appearance quality and the nutrition structure of rice, increases harvesting difficulty and reduces processing quality and commercial value of the rice. Researches show that the lodging can lead the yield loss of the rice to be 10% -30%, and even more than 50% in severe cases. The semi-dwarf or dwarf variety usually has the lodging-resistant function, so that the rice variety is improved by using a dwarf or semi-dwarf mutant gene or resource, and the rice plant height is reduced, and the method is the most main method for enhancing the lodging-resistant characteristic of the rice at present. However, excessive dwarfing generally causes the negative effects of small crop seeds, difficult heading, reduced photosynthetic efficiency, limited yield potential, reduced stress resistance, easy premature senility, difficult mechanized harvesting and the like, and especially the current dwarf and semi-dwarf genes generally reduce the plant height of rice by 30% -50%, and the plant height reducing effect is overlarge, so that the application of the dwarf and semi-dwarf genes in breeding is greatly limited. The known mutants of sixty short rods and semi-short rods can cause the reduction of important agronomic characters such as thousand seed weight and the like when the plant height is reduced, and particularly have poor fertility performance and poor applicability in actual variety improvement. Therefore, most of the dwarf and semi-dwarf genes are difficult to be utilized in agricultural production, particularly rice breeding. Since the last century, gibberellin-deleted semidwarf plant type genes (rice sd-1 genes) have been the most important resource for improving lodging resistance and increasing rice yield. With the large-scale application of a single gene in countries around the world, a series of scientific and social problems are caused. Therefore, there is an urgent need to develop new rice dwarf and semi-dwarf genes and germplasm resources with application value. Disclosure of Invention In order to solve the problems, three different fragments are selected from the SNR gene sequence of the wild rice as target sequences, gene knockout is carried out, and the invention discovers that the plant height of the rice can be gently reduced after the SNR gene is knocked out, good reproductive capacity of filial generation is maintained, and other agronomic characters such as thousand seed weight are not obviously changed. In order to achieve the above object, the first aspect of the present invention provides an SNR protein mutant, the amino acid sequence of which is any one of SEQ ID No.21, SEQ ID No.23, SEQ ID No.25. In a second aspect, the invention provides a nucleic acid molecule encoding any one of the SNR protein mutants shown in SEQ ID No.21, SEQ ID No.23, and SEQ ID No. 25. In a preferred scheme, the nucleotide sequence of the protein mutant shown as SEQ ID No.21 is shown as SEQ ID No.20, the nucleotide sequence of the protein mutant shown as SEQ ID No.23 is shown as SEQ ID No.22, and the nucleotide sequence of the protein mutant shown as SEQ ID No.25 is shown as