CN-122011147-A - IbTGA protein, coding gene and application thereof in regulation and control of sweet potato vine growth

Abstract

The invention belongs to the fields of plant genetic engineering technology and sweet potato breeding, and in particular relates to application of IbTGA genes in regulation and control of sweet potato vine growth, wherein the nucleotide sequence of the genes is shown as SEQ ID NO. 1. The invention proves that the over-expression IbTGA gene in the sweet potato can obviously increase the length between the stem nodes to promote the growth of the tendrils for the first time, and the expression of the gene is inhibited by the gene knockout technology to obviously shorten the length between the stem nodes to slow down the growth of the tendrils. Based on the above, the invention provides the application of creating a new sweet potato variety with moderate vine length and convenient mechanical harvest by regulating and controlling the expression abundance of IbTGA genes, and constructing IbTGA genes into an expression vector in specific implementation, and transforming sweet potato callus through agrobacterium mediation to obtain a transgenic plant. The discovery provides new gene resources and breeding technology for improving the aerial part property of sweet potatoes.

Inventors

• TANG WEI
• Zhang Aicen
• GAO RUNFEI
• LI QIANG
• ZHANG YUNGANG
• HOU MENG
• WANG XIN
• YAN HUI
• SONG WEIHAN
• LI CHEN
• GAO TIANQI

Assignees

• 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心)

Dates

Publication Date

20260512

Application Date

20260311

Claims (10)

1. 1. A IbTGA protein, which is derived from sweet potato and is the protein of the following (1) or (2): (1) A protein consisting of an amino acid sequence shown in SEQ ID NO. 2; (2) And (2) the protein which is derived from the protein in (1) and is related to plant stem growth through substitution and/or deletion and/or addition of one or more amino acid residues of the amino acid sequence shown in SEQ ID NO. 2.
2. 2. A gene encoding the IbTGA protein of claim 1.
3. 3. The coding gene according to claim 2, wherein the gene is any one of the following DNA molecules (1) to (3): (1) A DNA molecule shown in SEQ ID NO. 1; (2) A DNA molecule which hybridizes under stringent conditions with the DNA molecule defined in (1) and which encodes a plant stem growth-related protein; (3) A DNA molecule having at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% homology to the DNA sequence defined in (1) and encoding a plant stem growth related protein.
4. 4. Use of the IbTGA protein of claim 1 or the coding gene of claim 2 or 3 for regulating sweet potato vine growth by altering the expression level of the coding gene to effect an increase or decrease in sweet potato vine growth.
5. 5. The use according to claim 4, wherein the expression level of IbTGA gene is increased to increase sweet potato vine length.
6. 6. The use according to claim 4, wherein the expression level of IbTGA gene is suppressed to reduce sweet potato vine length.
7. 7. A method for adjusting sweet potato vine length, comprising any one of the following steps: (a) Over-expressing IbTGA gene in sweet potato to promote growth of sweet potato vine, or (B) Inhibiting IbTGA gene expression to slow sweet potato vine growth; Wherein the nucleotide sequence of IbTGA gene is shown as SEQ ID NO. 1.
8. 8. The method for regulating the vine length of sweetpotato as claimed in claim 7, wherein the over-expression in the step (a) comprises the steps of inserting the IbTGA gene into an expression vector to construct a recombinant plasmid, and transferring the recombinant plasmid into sweetpotato by genetic transformation.
9. 9. The method of claim 7, wherein in step (b), expression of IbTGA gene is inhibited by gene knockout.
10. 10. The method for regulating sweet potato vine length according to claim 8, wherein said genetic transformation adopts agrobacterium-mediated method and the infection receptor is sweet potato callus.

Description

IbTGA protein, coding gene and application thereof in regulation and control of sweet potato vine growth Technical Field The invention belongs to the fields of genetic engineering technology and sweet potato breeding, and in particular relates to IbTGA protein, a coding gene and application thereof in regulation and control of sweet potato vine growth. Background Sweet potatoes (Ipomoea batatas) are important grain crops for use as well in China, however, the mechanized harvesting process is greatly influenced due to long overground vines in production. In addition, the overlength tendrils can twine each other and overlap, lead to partial blade unable effective photosynthesis to influence the biomass accumulation of root tuber. Therefore, the sweet potato vine length can be effectively regulated and controlled, overgrowth can be prevented, and the method has important significance for improving the mechanical harvesting efficiency and yield of the sweet potato. Transcription factors play a key role in crop genetic engineering because their overexpression or interference can systematically up-regulate or down-regulate the gene clusters of growth development, thereby regulating key agronomic traits such as plant height. Among these, the TGA family is a plant-specific class of C2-C2 zinc finger proteins that exhibit a significant response to plant hormones, nutritional conditions, and a variety of abiotic stresses. Although their roles in stress adaptation and growth regulation have been established, there has been no study report so far on the regulation of sweet potato vine length by IbTGA. Disclosure of Invention The invention aims to provide IbTGA protein, a coding gene and application thereof in regulating and controlling the growth of sweet potato vines, so as to solve the problems in the prior art. The IbTGA protein and the coding gene thereof play an important role in regulating and controlling the growth of sweet potato vines, and provide a novel gene resource and breeding technology for improving the agronomic characters of sweet potatoes. In order to achieve the above object, the present invention provides the following solutions: the invention provides IbTGA protein related to regulation of sweet potato vine growth, which is derived from sweet potato and is the protein of the following (1) or (2): (1) A protein consisting of an amino acid sequence shown in SEQ ID NO. 2; (2) And (2) the protein which is derived from the protein in (1) and is related to plant stem growth through substitution and/or deletion and/or addition of one or more amino acid residues of the amino acid sequence shown in SEQ ID NO. 2. In order to achieve the aim, the invention also provides a coding gene of the IbTGA protein. Further, the gene is a DNA molecule of any one of the following (1) to (3): (1) A DNA molecule shown in SEQ ID NO. 1; (2) A DNA molecule which hybridizes under stringent conditions with the DNA molecule defined in (1) and which encodes a plant stem growth-related protein; (3) A DNA molecule having at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% homology to the DNA sequence defined in (1) and encoding a plant stem growth related protein. In order to achieve the above purpose, the invention also provides an application of IbTGA protein or the coding gene in regulating and controlling the growth of sweet potato vine length, wherein the regulation and control are realized by changing the expression level of the coding gene so as to increase or decrease the sweet potato vine length. Furthermore, the expression level of IbTGA genes is improved, so that the vine length of sweet potato is increased. Furthermore, the expression level of IbTGA gene is inhibited, so that the sweet potato vine length is reduced. The invention also provides a method for adjusting the vine length of the sweet potato, which comprises any one of the following steps: over-expressing IbTGA gene in sweet potato to promote growth of vine Expression of IbTGA genes was inhibited to slow tendril growth. Wherein the nucleotide sequence of IbTGA gene is shown as SEQ ID NO. 1. Further, the over-expression in the step (a) comprises the steps of inserting the IbTGA gene into a proper expression vector to construct a recombinant plasmid, and then transferring the recombinant plasmid into a sweet potato cell or tissue through genetic transformation. Further, in the step (b), the expression of IbTGA gene is suppressed by gene knockout (CRISPER CAS 9). Further, the genetic transformation adopts an agrobacterium-mediated method, wherein the infection receptor is preferably sweet potato callus. The technical scheme provided by the invention has the beneficial effect that IbTGA genes are key genes for regulating and controlling the vine length of sweet potatoes. The method provides an effective technical means for directionally changing the sweet potato vine length b