CN-122011158-A - Bovine serum albumin-derived multifunctional peptide with functions of antioxidation, blood glucose reduction and tyrosinase inhibition, and preparation and application thereof

Abstract

The invention discloses an antioxidant, hypoglycemic and tyrosinase inhibition multifunctional peptide derived from bovine serum albumin, and preparation and application thereof, belonging to the technical fields of foods, biological pharmacy and cosmetics. The invention screens and verifies the novel multifunctional peptide SFLYEYSRR with definite sequence, the highest clearance rate of the peptide SFLYEYSRR to ABTS reaches 89.93%, the highest clearance rate to DPPH free radical reaches 35.48%, the highest clearance rate to hydroxyl free radical reaches 46.49%, the inhibition rate to alpha-glucosidase reaches 64.36%, the highest inhibition activity to tyrosinase reaches 48.33%, and the application limitation of the existing single-function peptide is overcome.

Inventors

• TU MAOLIN
• Kang Zeyuan
• ZHANG MIAO
• PAN DAODONG
• SUN YANGYING
• DU MING

Assignees

• 宁波大学

Dates

Publication Date

20260512

Application Date

20260414

Claims (10)

1. 1. The application of the multifunctional peptide SFLYEYSRR in preparing food or health care products.
2. 2. The use according to claim 1, wherein the food product comprises a dairy product, a fruit and vegetable product, a meat product or a bean product.
3. 3. The application of multifunctional peptide SFLYEYSRR in preparing antioxidant product.
4. 4. The method of claim 3, wherein the product comprises a pharmaceutical product or a daily chemical product, and wherein the daily chemical product comprises a skin care product or a cosmetic product.
5. 5. The use according to claim 4, wherein the pharmaceutical product comprises a dosage form selected from the group consisting of tablets, capsules, injections, liposome nanoparticles, sustained release agents and dispersible tablets.
6. 6. The use of claim 5, wherein the pharmaceutical product comprises one or more of resistant dextrin, synthetic fruit and vegetable powder, fructo-oligosaccharides, probiotic freeze-dried powder, maltodextrin, citric acid, malic acid, stevioside, chromium picolinate, silica, perfume, and combinations thereof.
7. 7. The use according to claim 6, wherein the cosmetic formulation comprises a solution, a emulsion, a cream, a gel, a powder, an aerosol, a wax-based agent or a suspension.
8. 8. The use according to claim 7, wherein the cosmetic comprises one or more of a lotion, an emulsion, a cream or a soluble essence powder, and wherein the cosmetic comprises glycerin, dipropylene glycol, nicotinamide, acetylglucosamine, xanthan gum, ascorbyl glucoside, caprylic/capric triglyceride, polydimethylsiloxane, cetostearyl oleate, sorbitan oleate, tocopheryl acetate, ceramide, phenoxyethanol, and ethylhexyl glycerin.
9. 9. Use of a multifunctional peptide SFLYEYSRR in the manufacture of a medicament useful for maintaining blood glucose health levels.
10. 10. The application of the multifunctional peptide SFLYEYSRR in preparing a daily product for whitening and lightening spots is characterized in that the daily product comprises a skin care product or a cosmetic.

Description

Bovine serum albumin-derived multifunctional peptide with functions of antioxidation, blood glucose reduction and tyrosinase inhibition, and preparation and application thereof Technical Field The invention relates to an antioxidant, hypoglycemic and tyrosinase inhibition multifunctional peptide derived from bovine serum albumin, and preparation and application thereof, belonging to the technical fields of foods, biological pharmacy and cosmetics. Background In modern society, factors such as unhealthy life style, environmental pollution, aging, various diseases, etc., cause excessive generation of free radicals in human bodies, and induce oxidative stress. Oxidative stress is an important pathophysiological mechanism for the occurrence and progression of a variety of chronic diseases, including but not limited to cardiovascular disease, neurodegenerative disease, diabetes, cancer, and skin aging. For a long time, active search for natural or synthetic antioxidants capable of effectively scavenging free radicals and reducing oxidative damage has been conducted in order to prevent and treat these diseases closely related to oxidative stress. Diabetes, particularly type 2 diabetes, has become an increasingly serious public health challenge worldwide. The main characteristics are that the blood sugar metabolism disorder leads to the continuous rise of blood sugar level, long-term hyperglycemia not only can damage the functions of pancreatic beta cells, but also can cause systemic multi-organ complications, such as diabetic retinopathy, diabetic nephropathy, diabetic neuropathy, cardiovascular and cerebrovascular diseases, slow wound healing and the like, seriously influence the life quality of patients and bring about huge economic burden. At present, the treatment means of diabetes mainly comprise life style intervention, oral hypoglycemic drugs, insulin injection and the like, but the methods often have the limitations of poor curative effect, more side effects, poor compliance and the like. Therefore, the development of a safe, effective and low-side-effect new blood sugar-reducing strategy, in particular to a natural active ingredient capable of interfering blood sugar metabolism from multiple aspects, has important clinical significance and research value. In addition, in the field of skin health and beauty, abnormal pigment metabolism (e.g., chloasma, post-inflammatory pigmentation, etc.) is a general concern. Tyrosinase is a key rate-limiting enzyme in the melanin biosynthetic pathway, and its overactivation is a central mechanism leading to hyperpigmentation. Existing tyrosinase inhibitors (e.g. hydroquinone) are effective but often come with safety issues such as skin irritation, cytotoxicity, etc. Therefore, the development of novel, efficient and safe natural tyrosinase inhibitors has urgent market demands and application values. Whey protein is a main byproduct in the cheese production process, is a mixture of various proteins, and contains various bioactive proteins such as alpha-lactalbumin, beta-lactoglobulin, bovine serum albumin and the like. Through enzymolysis or microbial fermentation technology, various peptide segments with potential physiological activities, such as antioxidant peptide, antibacterial peptide, antihypertensive peptide and the like, can be released from whey protein, and have great application potential in the fields of foods, health-care products and medicines. In view of the above background, there is an urgent need to develop a novel whey protein peptide having synergistic antioxidant, hypoglycemic and tyrosinase inhibitory activities. The peptide has the remarkable capabilities of scavenging free radicals and protecting cells from oxidative damage, can effectively regulate the blood sugar level of an organism, improve insulin resistance and fundamentally prevent or relieve chronic diseases related to oxidative stress and hyperglycemia. Disclosure of Invention [ Technical problem ] The prior art has focused on increasing the degree of hydrolysis or general biological activity (e.g., antioxidant) of active peptides, and lacks directed release strategies for multifunctional peptides with antioxidant, hypoglycemic and tyrosinase inhibitory activities. Technical scheme Aiming at the problems, the invention firstly provides a multifunctional peptide SFLYEYSRR of bovine serum albumin source in whey protein with antioxidant, hypoglycemic and tyrosinase inhibitory activity effects, and the amino acid sequence of the multifunctional peptide SFLYEYSRR is Ser-Phe-Leu-Tyr-Glu-Tyr-Ser-Arg-Arg. The invention also provides application of the multifunctional peptide SFLYEYSRR in preparation of foods or health-care products. In one embodiment, the food product comprises a dairy product, a fruit and vegetable product, a meat product, or a soy product. The invention also provides application of the multifunctional peptide SFLYEYSRR in preparation of antioxidant products. In one embodiment