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CN-122011167-A - Monoclonal antibody for hepatitis B virus HBsAg and preparation and application thereof

CN122011167ACN 122011167 ACN122011167 ACN 122011167ACN-122011167-A

Abstract

The invention discloses a monoclonal antibody aiming at hepatitis B virus HBsAg, and preparation and application thereof, wherein the monoclonal antibody is one or any combination of SY-1 and SY-2, SY-3, SY-5, SY-6, SY-7, SY-8, SY-9, SY-10, SY-11, SY-12, SY-13, SY-14, SY-15, SY-16, SY-17, SY-18, SY-19, SY-20, SY-21, SY-22, SY-26, SY-27, SY-28, SY-29, SY-30, SY-31, SY-32, SY-33, SY-34, SY-35, SY-36, SY-37, SY-38, SY-39, SY-40, SY-41, SY-43, SY-44, SY-45, SY-46, SY-47, SY-48, SY-49, SY-50, SY-51, SY-52, SY-53, SY-54, SY-55, SY-56, SY-57. The monoclonal antibody obtained by screening has strong binding capacity with HBsAg, and has multiple biological functions and the capacity of identifying different HBV genotypes.

Inventors

  • ZHANG ZHENG
  • JU BIN
  • ZHOU BING
  • YAN HU
  • GE XIANGYANG
  • LI YUXIAO

Assignees

  • 深圳市第三人民医院(深圳市肝病研究所)

Dates

Publication Date
20260512
Application Date
20260206

Claims (9)

  1. 1. The monoclonal antibody aiming at the hepatitis B virus HBsAg is characterized in that the monoclonal neutralizing antibody is SY-1 and consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are SDSVSRGSYH, MQNSGHT and GRVGHDFWGGYLRFDYNTDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are TGAVTSGHS, DTS and LLSYSGLRV in sequence.
  2. 2. A monoclonal antibody composition directed against hepatitis b virus HBsAg, wherein the monoclonal neutralizing antibody composition is any combination of SY-1 and monoclonal antibody SY-2、SY-3、SY-5、SY-6、SY-7、SY-8、SY-9、SY-10、SY-11、SY-12、SY-13、SY-14、SY-15、SY-16、SY-17、SY-18、SY-19、SY-20、SY-21、SY-22、SY-26、SY-27、SY-28、SY-29、SY-30、SY-31、SY-32、SY-33、SY-34、SY-35、SY-36、SY-37、SY-38、SY-39、SY-40、SY-41、SY-43、SY-44、SY-45、SY-46、SY-47、SY-48、SY-49、SY-50、SY-51、SY-52、SY-53、SY-54、SY-55、SY-56、SY-57 of claim 1; The SY-2 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are SDSVSRGSYH, IQNSGST and GRLGHDFWSGYLRFDYNMDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are TGAVTSDHS, DTS and LLSYSGVRV in sequence; The SY-3 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are SDSVGRGSYF, MQNSGTT and GRQGHDFWSGYLRFDYNSDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are TGAVTSGHS, DTT and LLSYSGVRV in sequence; The SY-5 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GFSFSSYA, ISYDGSNT and AKEGNAFRYFGWLPDYQYFGMDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QSINRF, AAS and QQYGNTPLCS in sequence; the SY-6 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GFSFSSYA, ISYDGSNT and AKEGNAFRYFGWLSDYQYFGMDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QSINRY, AAS and QQYGNTPLCS in sequence; the SY-7 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GFSFSSYA, TSYDGSYT and AKEGNAFRYFGWLPDYQYYGMDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QSINRY, AAS and QQYGNSPLCS in sequence; the SY-8 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GFSFSSYA, ISYDGTTT and AKEGNAFRYFGLLPDYQYFGMDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QRINRF, AGS and QQYGNSPLCS in sequence; The SY-9 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GFSFSSYA, ISYDGTST and AKEGNAFRYFGFLPDYQYYGMDL in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QRINRF, AAS and QQYGNSPLCS in sequence; The SY-10 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GFSFTTYA, ISHDGNGK and AREFDCSGITCNTGRAFQY in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QSLLHSFNNEYH, WAS and QQYYSNPWT in sequence; the SY-11 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are VYSFQDTW, LSTQAAGATA and TTLFQGHFDY in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QSLLQRNGYNS, LGS and MQGLQTPYT in sequence; the SY-12 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are VYSFQDTW, LSTQAAGATA and TTLFQGHFDY in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QSLLQRNGYNS, LGS and MQGLQTPYT in sequence; the SY-13 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are VFSFRDVW, IKPKSGGGTS and ATFFQGYFDY in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QSLLHSNGYNY, FGS and MQALQTPYT in sequence; The SY-14 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are VFSFRDVW, IKPKSGGGTS and ATFFQGAFDY in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QSLLHSNGYNY, FGS and MQSLQTPYT in sequence; The SY-15 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are VFSFSNTW, IKSQPDGGTT and ATFFQGYFDY in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QSLLQSNGYNY, LGS and MQALQTPYT in sequence; The SY-16 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are VFSFTDAW, IKTKALGGTS and TTLFQGSFDY in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QTLMNRNGYNY, LSS and MQALQTPYT in sequence; the SY-17 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are VFSFTDAW, IKTKALGGTS and TTLFQGSFDY in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QTLMNRNGYNY, LSS and MQALQTPYT in sequence; the SY-18 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are VFSFSDAW, IKTKALGGTS and TTLFQGSFDY in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QTLMNRNGYNY, LSS and MQALQTPYT in sequence; the SY-19 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are VFDFTNAW, IRSNPNGGTA and VTQFLGSFDY in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QSLLHRNGRSY, LGS and MQALQPPYS in sequence; The SY-20 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GFTFNTYG, ISIGNSYK and AKLGVGYSSSSEDY in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are DSNIGNNY, KNN and AAWDNSLSGVI in sequence; The SY-21 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GFILSDHY, SRNKANSYTT and VRSILVTTGGNYFEC in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QGISSS, DAS and QQSKSYPLT in sequence; The SY-22 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GYTFTDYA, IDTSTGNP and VRGSAVHILRFFDWRRFDD in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are SSDVGGSNF, DVN and CSYAASYTWV in sequence; the SY-26 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are SDSVSRGSYH, MQNSGHT and GRVGHDFWGGYLRFDYNTDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are TGAVTSGHS, DTS and LLSYSGLRV in sequence; the SY-27 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are SDSVSRGSYH, MQNSGHT and GRVGHDFWGGYLRFDYNTDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are TGAVTSGHS, DTS and LLSYSGLRV in sequence; The SY-28 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are SDSVSRGSYH, MQNSGHT and GRVGHDFWGGYLRFDYNTDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are TGAVTSGHS, DTS and LLSYNGLRV in sequence; the SY-29 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are SDSVSRGSYY, VQNSGNT and GRVGHDFWGGYLRFDYNTDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are TGAVTSGHS, DTS and LLSYSGVRV in sequence; the SY-30 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are SDSVSRGSYY, VQNSGNI and GRVGHDFWGGYLRFDYNTDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are TGAVSNGHS, DTS and LLSYSGVRV in sequence; the SY-31 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are SDSVSRGSYH, IQNSGNT and GRVGHDFWGGYLRFDYNTDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are TGAVTSGHS, DTS and LLSYSGVRV in sequence; the SY-32 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are SDSVSRGSYF, IQNSGST and GRQGHDFWSGYLRFDYNTDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are TGAVTSGHS, DTN and LLSYSGVRV in sequence; the SY-33 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are SDSVGRGNYF, MQNSGTT and GRQGHDFWSGYLRFDYNSDI in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are TGAVTSGHS, DTT and LLSYSGVRV in sequence; The SY-34 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are SDSVGRGNYF, MQNSGTT and GRQGHDFWSGYLRFDYNSDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are TGAVTSGHS, DTT and LLSYSGVRV in sequence; the SY-35 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are SDSVGRGSYF, IQNSGST and GRQGHDFWSGYLRFDYNSDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are TGAVTSGHS, DTS and LLSYSGVRV in sequence; The SY-36 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are SDSVGRGNYF, MQNSGTT and GRQGHDFWSGYLRFDYNSDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are TGAVTSGHS, DTT and LLSYSGVRV in sequence; The SY-37 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are SDSVGRGSYF, MQNTGAT and GRMGHDFWSGYLRFDYNSDI in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are TGGVTSGHS, DTS and LLSYSGVRV in sequence; the SY-38 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GFTFRGYA, ISGSGLST and AKDPVSPVTVVGTFFQH in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are SSDVGGYNY, EVT and SSYAGSNTLGV in sequence; The SY-39 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GFTFRAYA, ISGSGLNT and AKDPVTPITVVGTFFLD in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are SNDVGGYNY, EVT and SSFAGSSNLGV in sequence; the SY-40 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GFTFRAYA, ISGSGLST and AKDPVTPITVVGTYFLD in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are SNDVGGYNY, EVT and SSFAGSSNLGV in sequence; the SY-41 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GFTFRAYA, ISGSGLNT and AKDPVTPITVVGTFFLD in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are SNDVGGYSY, EVT and SSFAGSSNLGV in sequence; the SY-43 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are DFSFSSHA, ISYDGGTT and AKEGNAFRYFGWLPDYQYFGMDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QRINRY, AAS and QQYGNSPLCS in sequence; The SY-44 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GFSFSSYA, ISYDGSNT and AKEGNAFRYFGWLPDYQYYGMDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QNINSRY, AAS and QQYGNSPLCS in sequence; The SY-45 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GFSFSSYA, ISYDGSNT and AKEGNAFRYFGWLPDYQYFGMDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QSINRY, AAS and QQYGNTPLCS in sequence; the SY-46 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are VYSFKDTW, LSTEAAGATA and TTLFQGHFDY in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QSLLQRNGYNS, LGS and MQGLQTPYT in sequence; The SY-47 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are VFDFNHVW, IKSKSDGATT and ATFFHAYFDY in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are RSLLQRNGDNY, LGT and QGLQTPYT in sequence; the SY-48 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are VFSFSDVW, IKPKSGGGTS and ATFFQGYFDY in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QSLLHSNGYNY, FGS and MQALQTPYT in sequence; The SY-49 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are VFSFSDAW, IKTTALGATS and TTLFGGAFDY in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QTVMNRNGYNY, LSS and MQSVQTPYT in sequence; The SY-50 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are EFTLSRHL, ISIGSSYI and VRLGVGFTSSSEDY in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are NSNIGSNY, KNN and AAWDNSLSGVA in sequence; The SY-51 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GFTFNRYG, ISIGSSYK and AKLGVGYSSSSEDY in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are DSNIGNNY, KNN and AAWDNSLGGVV in sequence; The SY-52 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GFTFSDHY, TRNRANSYRT and VRGPLVGKTGFDY in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QGIRNY, TVS and LQHSSYPYS in sequence; the SY-53 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are HFTFSSYA, IWYDGTYK and AKSLTGALEG in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QSLNSDDGNTY, TVS and MHRVEFPRT in sequence; the SY-54 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GYSFTNYA, IDTYTGNP and ARGSAVHILQFFDWRRFDD in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are SSDVGGYNF, DVS and CSYAGSYTWV in sequence; The SY-55 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GDTFSSYT, INGFLGIA and ARGDAGAVRLLYYNYAMDL in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QSLLQSDGKTY, EVS and MQTIQLPLT in sequence; The SY-56 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GGTFRSYG, IVPIFGIS and VRGGAVAGAGSAGEGIFDY in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QSVSKN, GAS and QQHHSWPPFT in sequence; The SY-57 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GYTFTKFG, ITPYNGNR and SRDGDRAAVSSF in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are ESLFFDSLNKNY, WAS and QQYIMSPWT in sequence.
  3. 3. The hepatitis b virus monoclonal neutralizing antibody composition of claim 2 wherein the heavy chain variable region sequence of SY-1 is Seq ID No.1 and the light chain variable region sequence is Seq ID No.2; The heavy chain variable region sequence of SY-2 is Seq ID No.3, and the light chain variable region sequence is Seq ID No.4; The heavy chain variable region sequence of SY-3 is Seq ID No.5, and the light chain variable region sequence is Seq ID No.6; The heavy chain variable region sequence of SY-5 is Seq ID No.7, and the light chain variable region sequence is Seq ID No.8; the heavy chain variable region sequence of SY-6 is Seq ID No.9, and the light chain variable region sequence is Seq ID No.10; the heavy chain variable region sequence of SY-7 is Seq ID No.11, and the light chain variable region sequence is Seq ID No.12; the heavy chain variable region sequence of SY-8 is Seq ID No.13, and the light chain variable region sequence is Seq ID No.14; The heavy chain variable region sequence of SY-9 is Seq ID No.15, and the light chain variable region sequence is Seq ID No.16; the heavy chain variable region sequence of SY-10 is Seq ID No.17, and the light chain variable region sequence is Seq ID No.18; the heavy chain variable region sequence of SY-11 is Seq ID No.19, and the light chain variable region sequence is Seq ID No.20; The heavy chain variable region sequence of SY-12 is Seq ID No.21, and the light chain variable region sequence is Seq ID No.22; the heavy chain variable region sequence of SY-13 is Seq ID No.23, and the light chain variable region sequence is Seq ID No.24; the heavy chain variable region sequence of SY-14 is Seq ID No.25, and the light chain variable region sequence is Seq ID No.26; the heavy chain variable region sequence of SY-15 is Seq ID No.27, and the light chain variable region sequence is Seq ID No.28; the heavy chain variable region sequence of SY-16 is Seq ID No.29, and the light chain variable region sequence is Seq ID No.30; the heavy chain variable region sequence of SY-17 is Seq ID No.31, and the light chain variable region sequence is Seq ID No.32; the heavy chain variable region sequence of SY-18 is Seq ID No.33, and the light chain variable region sequence is Seq ID No.34; the heavy chain variable region sequence of SY-19 is Seq ID No.35, and the light chain variable region sequence is Seq ID No.36; The heavy chain variable region sequence of SY-20 is Seq ID No.37, and the light chain variable region sequence is Seq ID No.38; The heavy chain variable region sequence of SY-21 is Seq ID No.39, and the light chain variable region sequence is Seq ID No.40; The heavy chain variable region sequence of SY-22 is Seq ID No.41, and the light chain variable region sequence is Seq ID No.42; the heavy chain variable region sequence of SY-26 is Seq ID No.43, and the light chain variable region sequence is Seq ID No.44; The heavy chain variable region sequence of SY-27 is Seq ID No.45, and the light chain variable region sequence is Seq ID No.46; The heavy chain variable region sequence of SY-28 is Seq ID No.47, and the light chain variable region sequence is Seq ID No.48; The heavy chain variable region sequence of SY-29 is Seq ID No.49, and the light chain variable region sequence is Seq ID No.50; the heavy chain variable region sequence of SY-30 is Seq ID No.51, and the light chain variable region sequence is Seq ID No.52; The heavy chain variable region sequence of SY-31 is Seq ID No.53, and the light chain variable region sequence is Seq ID No.54; the heavy chain variable region sequence of SY-32 is Seq ID No.55, and the light chain variable region sequence is Seq ID No.56; the heavy chain variable region sequence of SY-33 is Seq ID No.57, and the light chain variable region sequence is Seq ID No.58; The heavy chain variable region sequence of SY-34 is Seq ID No.59, and the light chain variable region sequence is Seq ID No.60; the heavy chain variable region sequence of SY-35 is Seq ID No.61, and the light chain variable region sequence is Seq ID No.62; the heavy chain variable region sequence of SY-36 is Seq ID No.63, and the light chain variable region sequence is Seq ID No.64; the heavy chain variable region sequence of SY-37 is Seq ID No.65, and the light chain variable region sequence is Seq ID No.66; the heavy chain variable region sequence of SY-38 is Seq ID No.67, and the light chain variable region sequence is Seq ID No.68; the heavy chain variable region sequence of SY-39 is Seq ID No.69, and the light chain variable region sequence is Seq ID No.70; The heavy chain variable region sequence of SY-40 is Seq ID No.71, and the light chain variable region sequence is Seq ID No.72; The heavy chain variable region sequence of SY-41 is Seq ID No.73, and the light chain variable region sequence is Seq ID No.74; The heavy chain variable region sequence of SY-43 is Seq ID No.75, and the light chain variable region sequence is Seq ID No.76; the heavy chain variable region sequence of SY-44 is Seq ID No.77, and the light chain variable region sequence is Seq ID No.78; the heavy chain variable region sequence of SY-45 is Seq ID No.79, and the light chain variable region sequence is Seq ID No.80; the heavy chain variable region sequence of SY-46 is Seq ID No.81, and the light chain variable region sequence is Seq ID No.82; the heavy chain variable region sequence of SY-47 is Seq ID No.83, and the light chain variable region sequence is Seq ID No.84; the heavy chain variable region sequence of SY-48 is Seq ID No.85, and the light chain variable region sequence is Seq ID No.86; The heavy chain variable region sequence of SY-49 is Seq ID No.87, and the light chain variable region sequence is Seq ID No.88; the heavy chain variable region sequence of SY-50 is Seq ID No.89, and the light chain variable region sequence is Seq ID No.90; the heavy chain variable region sequence of SY-51 is Seq ID No.91, and the light chain variable region sequence is Seq ID No.92; The heavy chain variable region sequence of SY-52 is Seq ID No.93, and the light chain variable region sequence is Seq ID No.94; the heavy chain variable region sequence of SY-53 is Seq ID No.95, and the light chain variable region sequence is Seq ID No.96; The heavy chain variable region sequence of SY-54 is Seq ID No.97, and the light chain variable region sequence is Seq ID No.98; the heavy chain variable region sequence of SY-55 is Seq ID No.99, and the light chain variable region sequence is Seq ID No.100; The heavy chain variable region sequence of SY-56 is Seq ID No.101, and the light chain variable region sequence is Seq ID No.102; The heavy chain variable region sequence of SY-57 is Seq ID No.103 and the light chain variable region sequence is Seq ID No.104.
  4. 4. A biomaterial, characterized in that the biomaterial is selected from one of the following (a) - (B): (A) A nucleic acid molecule encoding a monoclonal antibody directed against hepatitis b virus HBsAg of claim 1, or a monoclonal antibody composition directed against hepatitis b virus HBsAg of claim 3; (B) A recombinant plasmid and a recombinant cell containing the nucleic acid molecule of (A).
  5. 5. The biological material according to claim 4, wherein the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.1 is shown in Seq ID No.105, and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.2 is shown in Seq ID No. 106; The nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No. 3 is shown in Seq ID No.107, and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.4 is shown in Seq ID No. 108; The nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.5 is shown in Seq ID No.109, and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.6 is shown in Seq ID No. 110; The nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.7 is shown in Seq ID No.111, and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.8 is shown in Seq ID No. 112; The nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.9 is shown in Seq ID No.113 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.10 is shown in Seq ID No. 114; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.11 is shown in Seq ID No.115 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.12 is shown in Seq ID No. 116; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.13 is shown in Seq ID No.117 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.14 is shown in Seq ID No. 118; The nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.15 is shown in Seq ID No.119, and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.16 is shown in Seq ID No. 120; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.17 is shown in Seq ID No.121 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.18 is shown in Seq ID No. 122; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.19 is shown in Seq ID No.123 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.20 is shown in Seq ID No. 124; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.21 is shown in Seq ID No.125 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.22 is shown in Seq ID No. 126; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.23 is shown in Seq ID No.127 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.24 is shown in Seq ID No. 128; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.25 is shown in Seq ID No.129 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.26 is shown in Seq ID No. 130; The nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.27 is shown in Seq ID No.131, and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.28 is shown in Seq ID No. 132; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.29 is shown in Seq ID No.133 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.30 is shown in Seq ID No. 134; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.31 is shown in Seq ID No.135 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.32 is shown in Seq ID No. 136; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.33 is shown in Seq ID No.137, and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.34 is shown in Seq ID No. 138; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.35 is shown in Seq ID No.139 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.36 is shown in Seq ID No. 140; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.37 is shown in Seq ID No.141 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.38 is shown in Seq ID No. 142; The nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.39 is shown in Seq ID No.143 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.40 is shown in Seq ID No. 144; The nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.41 is shown in Seq ID No.145 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.42 is shown in Seq ID No. 146; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.43 is shown in Seq ID No.147 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.44 is shown in Seq ID No. 148; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.45 is shown in Seq ID No.149 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.46 is shown in Seq ID No. 150; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.47 is shown in Seq ID No.151 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.48 is shown in Seq ID No. 152; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.49 is shown in Seq ID No.153 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.50 is shown in Seq ID No. 154; The nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.51 is shown in Seq ID No.155 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.52 is shown in Seq ID No. 156; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.53 is shown in Seq ID No.157 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.54 is shown in Seq ID No. 158; The nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.55 is shown in Seq ID No.159 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.56 is shown in Seq ID No. 160; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.57 is shown in Seq ID No.161 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.58 is shown in Seq ID No. 162; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.59 is shown in Seq ID No.163, and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.60 is shown in Seq ID No. 164; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.61 is shown in Seq ID No.165 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.62 is shown in Seq ID No. 166; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.63 is shown in Seq ID No.167, and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.64 is shown in Seq ID No. 168; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.65 is shown in Seq ID No.169 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.66 is shown in Seq ID No. 170; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.67 is shown in Seq ID No.171 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.68 is shown in Seq ID No. 172; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.69 is shown in Seq ID No.173, and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.70 is shown in Seq ID No. 174; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.71 is shown in Seq ID No.175 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.72 is shown in Seq ID No. 176; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.73 is shown in Seq ID No.177 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.74 is shown in Seq ID No. 178; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.75 is shown in Seq ID No.179 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.76 is shown in Seq ID No. 180; The nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.77 is shown in Seq ID No.181, and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.78 is shown in Seq ID No. 182; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.79 is shown in Seq ID No.183, and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.80 is shown in Seq ID No. 184; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.81 is shown in Seq ID No.185 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.82 is shown in Seq ID No. 186; The nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.83 is shown in Seq ID No.187, and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.84 is shown in Seq ID No. 188; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.85 is shown in Seq ID No.189 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.86 is shown in Seq ID No. 190; The nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.87 is shown in Seq ID No.191, and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.88 is shown in Seq ID No. 192; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.89 is shown in Seq ID No.193, and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.90 is shown in Seq ID No. 194; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.91 is shown in Seq ID No.195, and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.92 is shown in Seq ID No. 196; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.93 is shown in Seq ID No.197 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.94 is shown in Seq ID No. 198; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.95 is shown in Seq ID No.199, and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.96 is shown in Seq ID No. 200; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.97 is shown in Seq ID No.201, and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.98 is shown in Seq ID No. 202; the nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.99 is shown in Seq ID No.203 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.100 is shown in Seq ID No. 204; The nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.101 is shown in Seq ID No.205 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.102 is shown in Seq ID No. 206; The nucleic acid sequence encoding the heavy chain variable region shown in Seq ID No.103 is shown in Seq ID No.207 and the nucleic acid sequence encoding the light chain variable region shown in Seq ID No.104 is shown in Seq ID No. 208.
  6. 6. The method for preparing the monoclonal antibody aiming at the hepatitis B virus HBsAg, which is characterized by comprising the steps of adopting the recombinant cell of claim 4 to carry out protein expression on the recombinant plasmid of claim 4, extracting and purifying the expressed protein, and obtaining the monoclonal antibody aiming at the hepatitis B virus HBsAg.
  7. 7. Use of a monoclonal antibody directed against hepatitis b virus HBsAg according to claim 1, or a monoclonal antibody composition directed against hepatitis b virus HBsAg according to any one of claims 2 to 3, or a biomaterial according to claim 4, in the preparation of a hepatitis b virus control drug or a hepatitis b virus detection reagent.
  8. 8. A hepatitis b virus controlling drug comprising the monoclonal antibody against hepatitis b virus HBsAg of claim 1 or the monoclonal antibody composition against hepatitis b virus HBsAg of any one of claims 2 to 3.
  9. 9. A hepatitis b virus detection reagent comprising the monoclonal antibody against hepatitis b virus HBsAg of claim 1 or the monoclonal antibody composition against hepatitis b virus HBsAg of any one of claims 2 to 3.

Description

Monoclonal antibody for hepatitis B virus HBsAg and preparation and application thereof Technical Field The application relates to the technical field of HBV antibodies, in particular to a monoclonal antibody aiming at hepatitis B virus HBsAg, and preparation and application thereof. Background Hepatitis b virus (HEPATITIS B VIRUS, HBV) infection remains a global significant public health problem. Chronic HBV infection can lead to a range of liver diseases such as chronic viral hepatitis b (chronic hepatitis B, CHB), cirrhosis (liver cirrhosis, LC), and primary hepatocellular carcinoma (hepatocellular carcinoma, HCC). There are two major classes of drugs currently approved by the FDA for the treatment of chronic hepatitis b, interferon and nucleoside analogs, which act as antiviral agents by modulating host immunity and inhibiting viral replication, respectively. The existing medicine has very strong safety and antiviral activity, but has very limited treatment effect. The negative conversion rate of the HBsAg of the medicines is less than 5%, and the clinical cure cannot be realized. Therefore, there is a need to develop more effective new drugs to improve clinical management of HBV-related diseases. The antibody plays an important role in preventing organism infection, helping organism remove viruses and the like, and can be used as a natural specific drug to be applied to the treatment research of chronic hepatitis B. Antibodies targeting viral surface antigens can on the one hand utilize Fab regions to prevent virus entry into hepatocytes by blocking the interaction of hepatitis b virus with pro-receptor HSPG, or to prevent a new round of viral infection by the antibody being endocytosed into hepatocytes followed by blocking release of hepatitis b virus from the infected cells. On the other hand, antibodies can also interact with phagocytes through the Fc region, mediating clearance of the antigen-antibody immune complex, i.e., the ADCP effect of the antibody. Although the traditional Hepatitis B Immunoglobulin (HBIG) is widely applied clinically, the traditional hepatitis B immunoglobulin is mainly derived from hepatitis B vaccine inoculator serum with higher antibody titer, the yield is low, and the antibodies from different batches have larger difference in concentration and titer. Therefore, there is a need to develop anti-hepatitis B virus monoclonal antibodies with higher titers and better specificity, and provide new means for treating chronic hepatitis B. Disclosure of Invention The invention aims to provide a monoclonal antibody aiming at hepatitis B virus HBsAg, and preparation and application thereof. In order to achieve the above purpose, the present invention adopts the following technical scheme: In a first aspect, the invention discloses a monoclonal antibody aiming at hepatitis B virus HBsAg, wherein the monoclonal neutralizing antibody is SY-1, and consists of a heavy chain and a light chain matched with the heavy chain, the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are SDSVSRGSYH, MQNSGHT and GRVGHDFWGGYLRFDYNTDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are TGAVTSGHS, DTS and LLSYSGLRV in sequence. In a second aspect the invention discloses a monoclonal antibody composition against hepatitis b virus HBsAg, characterized in that the monoclonal neutralising antibody composition is any combination of SY-1 and monoclonal antibody SY-2、SY-3、SY-5、SY-6、SY-7、SY-8、SY-9、SY-10、SY-11、SY-12、SY-13、SY-14、SY-15、SY-16、SY-17、SY-18、SY-19、SY-20、SY-21、SY-22、SY-26、SY-27、SY-28、SY-29、SY-30、SY-31、SY-32、SY-33、SY-34、SY-35、SY-36、SY-37、SY-38、SY-39、SY-40、SY-41、SY-43、SY-44、SY-45、SY-46、SY-47、SY-48、SY-49、SY-50、SY-51、SY-52、SY-53、SY-54、SY-55、SY-56、SY-57 as defined in claim 1; The SY-2 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are SDSVSRGSYH, IQNSGST and GRLGHDFWSGYLRFDYNMDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are TGAVTSDHS, DTS and LLSYSGVRV in sequence; The SY-3 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are SDSVGRGSYF, MQNSGTT and GRQGHDFWSGYLRFDYNSDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are TGAVTSGHS, DTT and LLSYSGVRV in sequence; The SY-5 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GFSFSSYA, ISYDGSNT and AKEGNAFRYFGWLPDYQYFGMDV in sequence, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain are QSINRF, AAS and QQYGNTPLCS in sequence; the SY-6 consists of a heavy chain and a light chain matched with the heavy chain, wherein the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain are GFSFSSYA, ISYDGSNT and AKEGNAFR