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CN-122011226-A - Mulberry oligosaccharide, preparation method thereof and application thereof in blood sugar regulation and control

CN122011226ACN 122011226 ACN122011226 ACN 122011226ACN-122011226-A

Abstract

The invention relates to mulberry oligosaccharide, a preparation method thereof and application thereof in blood sugar regulation. Through a great deal of researches, the mulberry oligosaccharide is obtained by treating the mulberry through specific enzymolysis, selenate modification, plasma treatment and other process steps, the stability of the mulberry oligosaccharide can be obviously improved, the inhibition capability of alpha-glucosidase is obviously improved, the glucose tolerance condition of diabetic mice is improved, the blood sugar level is stabilized, the insulin sensitivity of a human body can be improved, the insulin resistance degree is improved, the absorption and utilization of insulin to glucose are improved, the SOD activity of the human body is improved, the MDA content is reduced, and the development of diabetes is effectively slowed down. In addition, the mulberry fruit wine takes the mulberry fruit of natural sources as the main preparation raw material, does not contain any harmful chemical components, has low cost and good safety, and has great social significance.

Inventors

  • LI ERNA
  • LIU FAN
  • Pang Daorui
  • MU LIXIA
  • ZOU YUXIAO
  • ZHANG WEILONG

Assignees

  • 广东省农业科学院蚕业与农产品加工研究所

Dates

Publication Date
20260512
Application Date
20260130

Claims (10)

  1. 1. A preparation method of mulberry oligosaccharide is characterized by comprising the following steps: (1) Pulverizing Mori fructus, sieving, extracting with water, concentrating the extractive solution, precipitating with ethanol, recovering ethanol, and collecting precipitate to obtain Mori fructus polysaccharide; (2) The mulberry polysaccharide obtained in the step (1) is subjected to enzymolysis by glucanase, and then is subjected to enzymolysis by alkaline protease, so that enzymolysis liquid is obtained; (3) Adding the enzymolysis liquid obtained in the step (2) into a vitamin C solution for stirring reaction, and then adding H 2 O 2 for stirring to perform oxidative degradation reaction to obtain mulberry oligosaccharide; (4) Performing ultrafiltration separation on the mulberry oligosaccharide obtained in the step (3), taking a cut-off solution, adding a sodium selenite solution, a nitric acid solution and barium chloride into the cut-off solution, performing water bath heating reaction, removing the barium chloride after the reaction is finished, centrifuging to obtain a supernatant, adjusting the pH to be neutral, dialyzing to remove ions, changing water for 6-12 hours, and performing vacuum freeze drying to obtain solid powder of the selenate modified mulberry oligosaccharide; (5) And (3) placing the selenate modified mulberry oligosaccharide solid powder obtained in the step (4) into a groove of a reaction kettle for plasma treatment, thus obtaining the selenium acid modified mulberry oligosaccharide solid powder.
  2. 2. The preparation method according to claim 1, wherein the temperature of the enzymolysis with dextranase in the step (2) is 35-45 ℃, the time is 1-2h, the enzyme addition amount is 200U/mL, the temperature of the enzymolysis with alkaline protease is 40-50 ℃, the time is 2-3h, and the enzyme addition amount is 300U/mL.
  3. 3. The process according to claim 1, wherein the temperature of the stirring reaction in step (3) is 35 to 45 ℃ for 20 to 40 minutes.
  4. 4. The method according to claim 1, wherein the oxidative degradation reaction in step (3) is carried out at a temperature of 35 to 45 ℃ for a time of 40 to 80 minutes.
  5. 5. The preparation method of the mulberry oligosaccharide solution according to claim 1, wherein the ultrafiltration separation in the step (4) is specifically that macromolecular substances are removed through an ultrafiltration membrane with a molecular weight of 5kDa, effluent liquid is taken out, and then the retentate liquid is taken out through an ultrafiltration membrane with a molecular weight of 0.5kDa, so that the mulberry oligosaccharide solution with a molecular weight of 0.5-5kDa is obtained.
  6. 6. The method according to claim 1, wherein the water bath heating reaction in step (4) is carried out at a temperature of 50 to 70 ℃ for a time of 3 to 8 hours.
  7. 7. The method according to claim 1, wherein the dialysis is performed in step (4) using a dialysis bag having a molecular weight of 2000 Da.
  8. 8. The method of claim 1, wherein the plasma treatment in step (5) is performed by using dielectric barrier discharge as a low temperature plasma generation method, using an air environment as a reaction atmosphere, setting the diameter of the medium of the upper and lower round quartz plates to be 4-6cm, setting the discharge interval to be 6-10mm, setting the input voltage to be 100-130V, setting the input current to be 0.5-1.5A, and setting the treatment time to be 0.5-2min.
  9. 9. Mulberry oligosaccharide obtained by the preparation method according to any one of claims 1-8.
  10. 10. Use of mulberry oligosaccharide prepared by the preparation method according to any one of claims 1 to 8 in preparing a blood sugar regulating product.

Description

Mulberry oligosaccharide, preparation method thereof and application thereof in blood sugar regulation and control Technical Field The invention belongs to the field of natural product application, and particularly relates to mulberry oligosaccharide, a preparation method thereof and application thereof in blood sugar regulation. Background Diabetes is a metabolic disease characterized by hyperglycemia, and is one of the most important chronic diseases and main causes of death in modern society. In recent decades, the prevalence and incidence of diabetes has increased, with about 10% of people worldwide already suffering from or at risk of suffering from type 2 diabetes. In type 2 diabetics, alpha-glucosidase can hydrolyze starch to glucose during catabolism, resulting in a significant increase in postprandial blood glucose. Therefore, the effective components with the potential of treating diabetes mellitus are screened by carrying out alpha-glucosidase inhibition assay of natural plant components, and the method has become a common method for developing the hypoglycemic medicament without toxic or side effect. The plant resource polysaccharide has the functions of reducing blood sugar, reducing blood fat, resisting inflammation and the like, but has limited solubility in water due to overlarge molecular weight, and limits the application range. The macromolecular polysaccharide is hydrolyzed into oligosaccharide with smaller molecular weight, and the function of the oligosaccharide is enhanced by proper modification, so that the application field can be widened. The plant resource oligosaccharide extracted by the prior art only carries out simple mechanical crushing-solvent leaching, thermal reflux extraction or simple enzymolysis on plant resources, and the active oligosaccharide component is difficult to extract and cannot well play a role in regulating and controlling blood sugar. Therefore, the mulberry oligosaccharide which is high-efficiency in extraction, stable in effect and capable of regulating and controlling blood sugar is prepared by refining, extracting, degrading and modifying plant resource components, and has huge market potential. The plant resource oligosaccharide extracted by the prior art is simply crushed and mixed, or simply crushed and mixed mechanically, or extracted by solvent, extracted by thermal reflux or simply subjected to enzymolysis, and the active oligosaccharide component is difficult to extract and cannot well play a role in regulating and controlling blood sugar. Therefore, it is important to find mulberry oligosaccharide which is extracted efficiently, has stable effect and can regulate and control blood sugar by refining, extracting, degrading and modifying plant resource components. Disclosure of Invention Aiming at the problems that active oligosaccharide is difficult to effectively extract and the blood sugar regulating effect cannot be exerted to the greatest extent during mulberry extraction in the prior art, the invention provides a preparation method of mulberry oligosaccharide, which utilizes specific enzymolysis, selenate modification, plasma treatment and other process steps to treat mulberry, and develops the mulberry oligosaccharide which has stable performance, good safety and can effectively regulate and control blood sugar. To achieve the above object, the present invention is achieved by the following means: The first aspect of the invention provides a preparation method of mulberry oligosaccharide, which comprises the following steps: (1) Pulverizing Mori fructus, sieving, extracting with water, concentrating the extractive solution, precipitating with ethanol, recovering ethanol, and collecting precipitate to obtain Mori fructus polysaccharide; (2) The mulberry polysaccharide obtained in the step (1) is subjected to enzymolysis by glucanase, and then is subjected to enzymolysis by alkaline protease, so that enzymolysis liquid is obtained; (3) Adding the enzymolysis liquid obtained in the step (2) into a vitamin C solution for stirring reaction, and then adding H 2O2 for stirring to perform oxidative degradation reaction to obtain mulberry oligosaccharide; (4) Performing ultrafiltration separation on the mulberry oligosaccharide obtained in the step (3), taking a cut-off solution, adding a sodium selenite solution, a nitric acid solution and barium chloride into the cut-off solution, performing water bath heating reaction, removing the barium chloride after the reaction is finished, centrifuging to obtain a supernatant, adjusting the pH to be neutral, dialyzing to remove ions, changing water for 6-12 hours, and performing vacuum freeze drying to obtain solid powder of the selenate modified mulberry oligosaccharide; (5) And (3) placing the selenate modified mulberry oligosaccharide solid powder obtained in the step (4) into a groove of a reaction kettle for plasma treatment, thus obtaining the selenium acid modified mulberry oligosaccharide solid powd