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CN-122011227-A - Chlorella polysaccharide with tightening and anti-inflammatory effects, preparation method thereof and cosmetic

CN122011227ACN 122011227 ACN122011227 ACN 122011227ACN-122011227-A

Abstract

The application relates to the field of cosmetics, and relates to chlorella polysaccharide with a tightening and anti-inflammatory effect, a preparation method thereof and a cosmetic. Mixing chlorella powder with 1-5wt% of alkali solution, performing alkali extraction according to a feed liquid ratio of 1:10-1:50 g/mL to obtain a first extract, performing high-voltage pulse electric field extraction on the first extract, performing field intensity of 30-50 kV/m and pulse times of 20-80 times to obtain a second extract, performing heat extraction on the second extract in a water bath at 60-90 ℃, centrifuging, collecting supernatant, and performing alcohol precipitation on the supernatant. By adopting a synergistic extraction process of sequentially combining three steps of alkali extraction, high-voltage pulse electric field extraction and reheat extraction, the extraction efficiency of the chlorella polysaccharide is greatly improved, and meanwhile, the inhibition rate of the chlorella polysaccharide on collagenase and the inhibition rate of the chlorella polysaccharide on elastase are improved.

Inventors

  • SUN YUNQI
  • PEI YUNLIN
  • LEI TING
  • NIE YANFENG
  • GUO CHAOWAN
  • PU YAN

Assignees

  • 广东丸美生物技术股份有限公司

Dates

Publication Date
20260512
Application Date
20260209

Claims (10)

  1. 1. A method for preparing chlorella polysaccharide with compact anti-inflammatory effect, which is characterized by comprising the following steps: mixing chlorella powder with an alkali solution with the mass percentage concentration of 1% -5%, and performing alkali extraction according to a feed-liquid ratio of 1 g:10mL~1 g:50mL to obtain a first extract; extracting the first extracting solution by a high-voltage pulse electric field with the field intensity of 30-50 kV/m and the pulse times of 20-80 times to obtain a second extracting solution; Performing heat extraction on the second extracting solution in a water bath at 60-90 ℃, and collecting supernatant after centrifugation; And (3) carrying out alcohol precipitation on the supernatant.
  2. 2. The method for preparing chlorella polysaccharide with compact anti-inflammatory effect according to claim 1, wherein, The alkali solution comprises at least one of NaOH solution, KOH solution, na 2 CO 3 solution or NaHCO 3 solution.
  3. 3. The method for preparing chlorella polysaccharide with compact anti-inflammatory effect according to claim 1, wherein, The mass percentage concentration of the alkali solution is 3% -4%.
  4. 4. The method for preparing chlorella polysaccharide with compact anti-inflammatory effect according to claim 1, wherein, The field intensity extracted by the high-voltage pulse electric field is 40-50 kV/m, and the pulse times are 50-80 times.
  5. 5. The method for preparing chlorella polysaccharide with compact anti-inflammatory effect according to claim 1, wherein, The temperature of the heat extraction is 80-90 ℃, and optionally, the time of the heat extraction is 2-3 h.
  6. 6. The method for preparing chlorella polysaccharide with compact anti-inflammatory effect according to claim 1, wherein, The centrifugation condition is 5000-8000 r/min, and the centrifugation time is 10-20 min.
  7. 7. The method for producing a chlorella polysaccharide with a compact anti-inflammatory effect according to any one of claims 1 to 6, characterized in that, The preparation method of the chlorella powder comprises the steps of culturing chlorella in a culture medium under the conditions of 20-30 ℃ and 3000-4000 lux illumination intensity and alternate light and dark, taking algae liquid cultured to a growth stabilization period, centrifuging the algae liquid, collecting algae mud, and freeze-drying the algae mud.
  8. 8. The chlorella polysaccharide is characterized in that the chlorella polysaccharide with the tightening and anti-inflammatory effects is prepared by the preparation method of any one of claims 1-7.
  9. 9. The chlorella polysaccharide according to claim 8, wherein, The inhibition rate of the chlorella polysaccharide to collagenase is 9.8% -10.6%; Optionally, the inhibition rate of the chlorella polysaccharide to elastase is 40.4% -45.2%.
  10. 10. A cosmetic comprising the chlorella polysaccharide of claim 8 or 9.

Description

Chlorella polysaccharide with tightening and anti-inflammatory effects, preparation method thereof and cosmetic Technical Field The application relates to the field of cosmetics, in particular to chlorella polysaccharide with a tightening and anti-inflammatory effect, a preparation method thereof and a cosmetic. Background The chlorella polysaccharide has various biological activities such as antioxidation, immunoregulation and the like, and has good application potential in the fields of food, health care products and cosmetics. However, the chlorella polysaccharide is difficult to extract, the yield is low, and the traditional extraction process is difficult to combine high extraction efficiency and high biological activity of the product. There is no clear definition of which specific effects the chlorella polysaccharide is associated with. Disclosure of Invention The embodiment of the application aims to provide chlorella polysaccharide with a tightening and anti-inflammatory effect, a preparation method thereof and cosmetics. In a first aspect, the present application provides a method for preparing chlorella polysaccharide with a compact anti-inflammatory effect, comprising: mixing chlorella powder with an alkali solution with the mass percentage concentration of 1% -5%, and performing alkali extraction according to the feed liquid ratio of 1:10-1:50 g/mL to obtain a first extract; Extracting the first extracting solution by a high-voltage pulse electric field with the field intensity of 30-50 kV/m and the pulse times of 20-80 times to obtain a second extracting solution; Performing heat extraction on the second extract in a water bath at 60-90 ℃, centrifuging, and collecting supernatant; And (5) carrying out alcohol precipitation on the supernatant. According to the technical scheme, the three-step sequential combined synergistic extraction process of alkali extraction, high-voltage pulse electric field extraction and reheat extraction is adopted, the cell wall is softened through alkali extraction, then non-thermal perforation of the pulse electric field is carried out to promote release of intracellular polysaccharide, thermal extraction is further carried out to promote dissolution of polysaccharide, and the three sequences are synergistic, so that the extraction efficiency of chlorella polysaccharide is greatly improved, the inhibition rate of chlorella polysaccharide to collagenase is improved, and the inhibition rate of chlorella polysaccharide to elastase is improved. In other embodiments of the present application, the alkali solution comprises at least one of NaOH solution, KOH solution, na 2CO3 solution or NaHCO 3 solution. In other embodiments of the present application, the concentration of the alkaline solution is 3% -4% by mass. In other embodiments of the present application, the field strength extracted by the high-voltage pulse electric field is 40-50 kV/m, and the pulse times are 50-80 times. In other embodiments of the application, the temperature of the heat extraction is 80-90 ℃, optionally the time of the heat extraction is 2-3 hours. In other embodiments of the application, the centrifugation conditions are 5000-8000 r/min and the centrifugation time is 10-20 min. In other embodiments of the application, the preparation of chlorella powder comprises culturing chlorella in a culture medium under alternating conditions of 20-30 ℃ and 3000-4000 lux illumination intensity and darkness, taking algae liquid cultured until the growth is stable, centrifuging the algae liquid, collecting algae mud, and freeze-drying the algae mud. In a second aspect, the present application provides a chlorella polysaccharide, which is prepared by using the preparation method of the chlorella polysaccharide with the tightening anti-inflammatory effect provided by any one of the embodiments of the second aspect. In other embodiments of the application, the inhibition rate of chlorella polysaccharide to collagenase is 9.8% -10.6%; in other embodiments of the application, the inhibition rate of chlorella polysaccharide to elastase is 40.4% -45.2%. In a third aspect, the present application provides a cosmetic comprising the Chlorella polysaccharide provided in the second aspect. Drawings In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. FIG. 1 is a graph of anti-inflammatory activity of Chlorella polysaccharide. Detailed Description For the purpose of making the objects, technical solutions and advantages of the embodiments of the present application more apparent, the technica