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CN-122011229-A - Cinnamon branch and leaf polysaccharide with blood sugar reducing characteristic, and preparation method and application thereof

CN122011229ACN 122011229 ACN122011229 ACN 122011229ACN-122011229-A

Abstract

The invention discloses cinnamon branch and leaf polysaccharide with blood sugar reducing property, and a preparation method and application thereof, and belongs to the technical field of biological medicines. The invention takes cinnamon branches and leaves as a byproduct of cinnamon processing as raw materials, carries out hot water extraction, deproteinization by a Sevage method, alcohol precipitation, and further carries out purification by DEAE-cellulose ion exchange chromatography and Sephacryl gel filtration chromatography to obtain cinnamon branches and leaves polysaccharide. The monosaccharide composition of the cinnamon branch and leaf polysaccharide mainly comprises galacturonic acid, galactose and rhamnose, and the glycosidic bond connection analysis shows that the main chain is mainly connected with 4-GalpA, and the cinnamon branch and leaf polysaccharide is of a typical pectin polysaccharide structure. In a type 2 diabetes mouse model, the cinnamon branch and leaf polysaccharide provided by the invention can obviously reduce fasting blood glucose, improve glucose tolerance and insulin resistance, regulate dyslipidemia, and has a protective effect on liver, kidney and pancreas.

Inventors

  • ZHENG JIAXIN
  • ZHANG HUIKUN
  • SONG MINGYUE

Assignees

  • 华南农业大学

Dates

Publication Date
20260512
Application Date
20260325

Claims (10)

  1. 1. The cinnamon twig and leaf polysaccharide is characterized in that the preparation method comprises the following steps: S1, placing cinnamon branches and leaves in water, leaching with hot water, and concentrating the extracting solution under reduced pressure to obtain a first concentrated solution; S2, deproteinizing the first concentrated solution, and carrying out alcohol precipitation treatment to obtain crude polysaccharide of cinnamon branches and leaves; S3, redissolving crude polysaccharide of cinnamon branches and leaves, eluting through a DEAE-52 cellulose chromatographic column, sequentially carrying out gradient elution by using water, 0.1mol/LNaCl, 0.2mol/LNaCl and 0.3mol/LNaCl solution, collecting main peak components in the eluting stage of 0.1mol/LNaCl, and concentrating under reduced pressure to obtain a second concentrated solution; S4, eluting the second concentrated solution by SEPHACRYL S-400 gel filtration chromatographic columns, eluting by water, concentrating under reduced pressure, and freeze-drying to obtain the cinnamon twig and leaf polysaccharide.
  2. 2. The cinnamon twig and leaf polysaccharide as set forth in claim 1, wherein said deproteinizing comprises adding said first concentrate to a Sevage reagent, shaking and standing, and leaving the aqueous phase until no significant protein layer is present at the interface of the aqueous phase and the organic phase.
  3. 3. The cinnamon twig and leaf polysaccharide according to claim 1, wherein the alcohol precipitation treatment comprises adding ethanol into the deproteinized aqueous phase, standing at a low temperature, separating, and freeze-drying the precipitate to obtain the cinnamon twig and leaf crude polysaccharide.
  4. 4. The cinnamon branch and leaf polysaccharide according to claim 1, wherein the monosaccharide composition of the cinnamon branch and leaf polysaccharide is 37.32% galacturonic acid, 18.16% galactose, 15.18% rhamnose, 14.32% arabinose, 6.3% xylose, 4.14% mannose, 2.14% glucuronic acid, 1.72% glucose and 0.73% fucose in mole percent.
  5. 5. The cinnamon twig and leaf polysaccharide according to claim 1, wherein the cinnamon twig and leaf polysaccharide has a weight average molecular weight Mw of 12.343kDa, a number average molecular weight Mn of 7.980kDa and a dispersion coefficient of 1.547.
  6. 6. The cinnamon twig and leaf polysaccharide according to claim 1, wherein the total sugar content of the cinnamon twig and leaf polysaccharide is 63.80 +/-8.96% and the uronic acid content is 30.59 +/-1.85% in mass percent.
  7. 7. The cinnamon twig and leaf polysaccharide according to claim 1, wherein the cinnamon twig and leaf polysaccharide is connected in a manner of between 4 and GalpA, between 4 and Galp, between t and GalpA and between 2 and Rhap, and the molar percentages are respectively 32.05%, 14.64%, 11.04% and 6.30%.
  8. 8. The application of the cinnamon twig and leaf polysaccharide of any one of claims 1-7 in preparing hypoglycemic drugs and/or health-care foods.
  9. 9. The use according to claim 8, characterized in that the cinnamon twig and leaf polysaccharide is used for preparing hypoglycemic drugs and/or health foods with the function of improving insulin resistance.
  10. 10. A hypoglycemic agent, characterized by comprising the cinnamon twig and leaf polysaccharide according to any one of claims 1 to 7.

Description

Cinnamon branch and leaf polysaccharide with blood sugar reducing characteristic, and preparation method and application thereof Technical Field The invention relates to the technical field of biological medicines, in particular to cinnamon twig and leaf polysaccharide with blood sugar reducing property, and a preparation method and application thereof. Background Cinnamon is a camphoraceae plant, and bark of cinnamon is a traditional medicine and food dual-purpose raw material in China, and is widely used in the fields of seasonings, food additives and traditional medicines. Modern pharmacological researches show that cinnamon has various biological activities of resisting oxidation, resisting inflammation, regulating glycolipid metabolism and the like, and main active ingredients of cinnamon comprise volatile oil, polyphenols and polysaccharide substances. The cinnamon resources in China are rich, the annual output of cinnamon oil is more than 2000 tons, and the residual branches and leaves (commonly called cinnamon residues) of the cinnamon produced by the cinnamon are about 20 ten thousand tons. These byproducts are currently mostly discarded or used as fuel, resulting in serious resource waste and environmental stress. Researches show that the cinnamon leaf residue still contains abundant active substances, wherein the polysaccharide content can reach 24.2%. CN106279469a discloses a preparation method of crude polysaccharide from cinnamon residue, which adopts hot water extraction and combined composite enzymolysis treatment, so that the extraction rate of the crude polysaccharide is improved. However, the technical scheme still stays in the preparation stage of crude polysaccharide, the purity of the obtained product is low, further separation and purification, structure identification and activity evaluation of a system are not involved, the polysaccharide component with fine analysis of the structure is not obtained, and the functional components and the action mechanism of the polysaccharide component are difficult to be clarified. In the evaluation of hypoglycemic activity, the existing research is mainly focused on the in vitro enzyme inhibition level. In the prior in vivo study, crude polysaccharide is mostly adopted as a test object, and definition of fine structural characteristics of the crude polysaccharide is lacking, so that the activity evaluation result is difficult to be related with specific structural characteristics. Disclosure of Invention In order to solve the problems of the prior art, the primary object of the present invention is to provide a cinnamon twig and leaf polysaccharide. The invention also aims to provide application of the cinnamon twig and leaf polysaccharide in preparation of hypoglycemic drugs and/or health-care foods. It is still another object of the present invention to provide a hypoglycemic agent comprising the cinnamon twig and leaf polysaccharide as described above. In order to achieve the above object, the present invention provides the following technical solutions: The preparation method of the cinnamon twig and leaf polysaccharide comprises the following steps: S1, placing cinnamon branches and leaves in water, leaching with hot water, and concentrating the extracting solution under reduced pressure to obtain a first concentrated solution; S2, deproteinizing the first concentrated solution, and carrying out alcohol precipitation treatment to obtain crude polysaccharide of cinnamon branches and leaves; S3, redissolving crude polysaccharide of cinnamon branches and leaves, eluting through a DEAE-52 cellulose chromatographic column, sequentially carrying out gradient elution by using water, 0.1mol/L NaCl, 0.2mol/L NaCl and 0.3mol/L NaCl solution, collecting main peak components in the 0.1mol/L NaCl elution stage, and concentrating under reduced pressure to obtain a second concentrated solution; S4, eluting the second concentrated solution by SEPHACRYL S-400 gel filtration chromatographic columns, eluting by water, concentrating under reduced pressure, and freeze-drying to obtain the cinnamon twig and leaf polysaccharide. Further, the pretreatment method of the cinnamon branches and leaves comprises the steps of removing impurities from the cinnamon branches and leaves, crushing and sieving to obtain cinnamon branch and leaf powder. Further, the temperature of the hot water leaching is 70-100 ℃. Preferably 90 ℃. Further, the hot water leaching time is 1-4 hours. Preferably 2h. Further, the hot water leaching times are 1-3 times. Preferably 2 times. Further, the temperature of the reduced pressure concentration is 50-70 ℃. Preferably 60 ℃. Further, the volume of the first concentrated solution is 1/12-1/8 of the volume of the extracting solution. Preferably 1/10. Further, the deproteinization treatment comprises adding the first concentrated solution into a Sevage reagent, shaking and standing, and retaining the aqueous phase until the interface between the a