CN-122011232-A - Method for extracting polyphenol from pear by-product

Abstract

The invention belongs to the technical field of fruit byproduct processing, and particularly relates to a method for extracting polyphenol from pear byproducts. The invention utilizes the thermomechanical and chemical effects of steam explosion to carry out steam explosion treatment on the pear byproducts, can remove most hemicellulose and lignin, efficiently and rapidly extract cellulose, pectin and polyphenol, shortens the production cycle of the cellulose, the pectin and the polyphenol and improves the efficiency, and compared with other types of hydrogen bond acceptors and hydrogen bond donors, the invention takes the combination of choline chloride and ethanol as a eutectic solvent, has the most obvious polyphenol extraction effect, and can furthest improve the extraction efficiency of the pear byproduct polyphenol through a simple process. The method lays a foundation for further development and utilization of pear residue cellulose, pectin and polyphenol, and improves the comprehensive utilization rate of pear processing byproducts.

Inventors

• TIAN GUIFANG
• DU KUN
• SANG YAXIN
• LI JIANGTAO
• GE JING
• CUI YIFAN
• YUAN FENG

Assignees

• 河北农业大学

Dates

Publication Date

20260512

Application Date

20241104

Claims (6)

1. 1. A method for extracting polyphenol from pear by-products, which is characterized by comprising the following steps: Sequentially carrying out steam explosion treatment and crushing treatment on the pear byproducts to obtain pear residue powder, wherein the steam explosion pressure of the steam explosion treatment is 0.3-2.0 MPa; Extracting polyphenol from the pear residue powder; The polyphenol extraction step comprises the steps of mixing pear residue powder with a eutectic solvent solution, carrying out ultrasonic extraction, and collecting an extracting solution, wherein the extracting solution contains pear polyphenol, the eutectic solvent solution comprises a hydrogen bond acceptor and a hydrogen bond donor, the hydrogen bond acceptor comprises choline chloride, the hydrogen bond donor comprises ethanol, and the molar ratio of the choline chloride to the ethanol is 1 (1-3).
2. 2. The method according to claim 1, wherein the steam explosion treatment is performed for 1 to 5 minutes.
3. 3. The method according to claim 2, wherein the medium for steam explosion treatment is saturated steam, and the temperature of the saturated steam is 160-230 ℃.
4. 4. The method according to claim 1, wherein the mass-to-volume ratio of the pear residue powder to the eutectic solvent solution is 1g (15-35) mL.
5. 5. The method of claim 1, wherein the mixture of the powder of pear by-product and the eutectic solvent solution has a moisture content of 20-60%.
6. 6. The method according to claim 1, wherein the ultrasonic extraction time is 20-60 min and the power is 300-700W.

Description

Method for extracting polyphenol from pear by-product The application relates to a divisional application of 2024, 11-04, 202411558071.3 and method for extracting cellulose, pectin and polyphenol from pear by-products, which is the agricultural university of Hebei. Technical Field The invention belongs to the technical field of fruit byproduct processing, and particularly relates to a method for extracting polyphenol from pear byproducts. Background The pear by-product is waste by-product produced by juice making, drying, canning and the like by taking fresh pear as a raw material, and mainly comprises pear peel, pear pulp, pear core, fruit stalks and the like, wherein the total amount of the pear by-product is about 40% -50% of the weight of the original fresh pear. Because the pear residues contain partial stone cells, the rough processing palatability is poor, the recycling rate is low, the pear residues are usually treated as waste, and the fresh pear residues are easy to rot and deteriorate if not immediately treated, so that the pear residues are mildewed to influence the utilization of the pear residues, and huge troubles are brought to production enterprises and the environment. However, pear residues contain a large amount of polysaccharide, phenolic substances, flavone, cellulose, hemicellulose and other substances, and are good sources of high-quality bioactive components. Cellulose is a green sustainable material that can be used in the manufacture of many high value products including regenerated cellulose, cellulose ethers, esters and nanocrystalline cellulose. Cellulose and hemicellulose separation technology has been a hot topic of the research community. At present, common extraction methods of cellulose are a physical method and a chemical method, the former main methods are high-pressure cooking and microwave treatment, a pretreatment method of crushing materials and then utilizing sodium hydroxide solution is adopted, and then the cooking or the microwave treatment is carried out under the high-temperature and high-pressure environment. But the cellulose impurities are more and the extraction rate is low. In addition, the high-pressure cooking method mostly uses large-scale cooking equipment, and has the advantages of high power consumption, overlarge pressure and operation danger, and chemical medicines used by the chemical method are easy to cause secondary pollution to the environment. These modes produce a large amount of pollution and cellulose is susceptible to damage. Pectin is a plant acidic polysaccharide with poly alpha-1, 4-D-galacturonan as a framework component, and has good gelation and emulsion stability, and has become an important natural additive in the food industry, medicine, daily chemicals and textile industry. At present, the extraction method of pectin mainly comprises acid extraction, enzyme extraction, salt extraction and the like. The most commonly used extraction method is acid extraction, generally, raw pectin is hydrolyzed into water-soluble pectin under the condition of heating in acid solution with a certain pH value to form pectin aqueous solution, and enzyme extraction method is to remove substances such as cellulose, hemicellulose and the like in cell walls by utilizing enzymes such as cellulase, hemicellulase and the like to degrade macromolecular substances in pectin or convert insoluble pectin into water-soluble pectin, and then extract the pectin. However, the enzymatic extraction of pectin usually takes more than 10 hours, and the salt extraction usually adopts ammonium oxalate to change insoluble calcium pectate into soluble ammonium salt, thereby increasing the solubility of insoluble pectin. The existing method has the defects of low pectin yield and incomplete extraction. Polyphenols, which are a major class of secondary metabolites, are almost present in a variety of plants, with potential health promoting effects. Currently polyphenol extraction is usually carried out by ethanol extraction, concentration purification and ethyl acetate extraction, and crude product purification by acetone. The method has complicated steps, the purity of polyphenol is increased, but the yield is lower, and the extract is limited in application due to the unavoidable existence of reagent residues in the extract due to the adoption of organic solvents such as ethyl acetate, acetone and the like for extraction. Disclosure of Invention The invention aims to provide a method for extracting cellulose, pectin and polyphenol from pear byproducts, which improves the extraction rate of the cellulose, the pectin and the polyphenol in the pear byproducts, and the obtained cellulose has uniform particle size distribution, large length-diameter ratio and high crystallinity, the pectin has the effect of adjusting anti-inflammatory activity, the polyphenol has antioxidant activity, improves the utilization rate of the pear processing byproducts, and has simple process and envi