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CN-122011430-A - Tremella polysaccharide-based hydrogel microsphere and preparation method and application thereof

CN122011430ACN 122011430 ACN122011430 ACN 122011430ACN-122011430-A

Abstract

The invention provides tremella polysaccharide-based hydrogel microspheres, a preparation method and application thereof, and belongs to the field of functional polysaccharide materials. The preparation method of the hydrogel microsphere comprises the following steps of (1) preparing a water phase containing tremella polysaccharide and cationic polymer, (2) preparing an oil phase containing surfactant, (3) preparing water-in-oil droplets through a capillary glass tube single-stage microfluidic device, and (4) introducing the oil phase containing the water-in-oil droplets into a receiving system containing acetic acid for crosslinking. The hydrogel microsphere can be prepared by adopting a simple device, the preparation process is mild, the preparation is biologically friendly, and the obtained hydrogel microsphere has uniform particle size and good monodispersity. Meanwhile, the hydrogel microsphere prepared by the method has rich autofluorescence characteristics, can be used as a fluorescent tracing carrier or an imaging material for carrying various active substances, omits an exogenous dye loading step, reduces toxicity, simplifies the preparation process, and has good application prospect.

Inventors

  • MENG ZHIJUN
  • WANG TIANLIANG
  • HU BAIREN
  • ZHANG CHUAN
  • LI MING
  • HE JIAN

Assignees

  • 川北医学院

Dates

Publication Date
20260512
Application Date
20251218

Claims (10)

  1. 1. The method for preparing the tremella polysaccharide-based hydrogel microsphere is characterized by comprising the following steps of: (1) Preparing a water phase containing tremella polysaccharide and cationic polymer; (2) Preparing an oil phase containing a surfactant; (3) Preparing water-in-oil droplets through a capillary glass tube single-stage microfluidic device; (4) Introducing the oil phase containing the water-in-oil droplets into a receiving system for crosslinking to obtain the tremella polysaccharide-based hydrogel microsphere, wherein the receiving system is the oil phase containing acetic acid.
  2. 2. The method according to claim 1, characterized in that: In the step (1), the preparation method of the water phase comprises the following steps: (a) Preparing tremella polysaccharide aqueous solution with mass fraction of 0.05-1.5wt%; (b) Preparing a cationic polymer aqueous solution with the mass fraction of 0.1-4.0wt%; (c) Mixing tremella polysaccharide aqueous solution and cationic polymer aqueous solution according to the volume ratio of (1-5): 1, and regulating the pH value to 5.7-6.4 to obtain a water phase; And/or, in the step (2), the preparation method of the oil phase comprises the following steps: Adding a surfactant into a continuous phase, and uniformly mixing to obtain an oil phase with the mass fraction of 0.5-10.0wt% of the surfactant, wherein the continuous phase is selected from soybean oil, mineral oil, liquid paraffin, silicone oil or fluorine oil, and the surfactant is selected from polyglycerol polyricinoleate, span80 or special surfactant for fluorine oil.
  3. 3. The method according to claim 2, characterized in that: In the step (a), the mass fraction of the tremella polysaccharide water solution is 1.0wt%; and/or in the step (b), the mass fraction of the cationic polymer aqueous solution is 2.0-4.0wt%; And/or, in the step (c), the volume ratio of the tremella polysaccharide water solution to the cationic polymer water solution is 3:1; and/or, in step (c), said adjusting the pH to 5.9; and/or the mass fraction of surfactant in the oil phase is 5.0 wt%; and/or, the continuous phase is selected from soybean oil; and/or the surfactant is selected from polyglycerol polyricinoleate.
  4. 4. A method according to claim 3, characterized in that: in step (b), the cationic polymer is chitosan; and/or, in step (c), the pH is adjusted using an aqueous sodium hydroxide solution having a concentration of 1.0 wt%.
  5. 5. The method according to claim 1, characterized in that: In the step (3), when water-in-oil droplets are prepared, the flow rate of the water phase is 10-1500 mu L/h, and the flow rate of the oil phase is 200-10000 mu L/h; And/or in the step (4), the volume fraction of acetic acid in the oil phase containing acetic acid is 0.05-0.5 v/v%; and/or, in the step (4), the cross-linking time is 10-120 min.
  6. 6. The method of claim 1, wherein the aqueous phase in the step (1) contains or does not contain genipin, and the mass fraction of genipin is 0.01-0.5wt% when genipin is contained.
  7. 7. The method according to claim 6, wherein: when the aqueous phase does not contain genipin, in the step (4), the crosslinking is crosslinking in an oil phase containing acetic acid; when the water phase contains genipin, in the step (4), the crosslinking is crosslinking in an oil phase containing acetic acid; When the water phase contains genipin, in the step (4), the crosslinking is carried out by standing in an oil phase without acetic acid, and then crosslinking in the oil phase with acetic acid.
  8. 8. The method according to claim 1, wherein in the step (4), the excess oil phase is washed with normal ethane after the tremella polysaccharide-based hydrogel microspheres are obtained, and finally, the normal ethane is washed with deionized water.
  9. 9. A tremella polysaccharide-based hydrogel microsphere is characterized by being prepared by the method of any one of claims 1-8.
  10. 10. Use of the tremella polysaccharide-based hydrogel microsphere of claim 9 in the fields of food engineering, drug delivery, cell labeling, bioimaging or fluorescence sensing.

Description

Tremella polysaccharide-based hydrogel microsphere and preparation method and application thereof Technical Field The invention belongs to the field of functional polysaccharide materials, and particularly relates to tremella polysaccharide-based hydrogel microspheres, and a preparation method and application thereof. Background Tremella polysaccharide is a natural fungus polysaccharide with wide sources, has good biological activities such as biocompatibility, immunoregulation and antioxidation, and is widely focused in the fields of functional foods, drug delivery, tissue engineering and the like in recent years. Therefore, developing the monodisperse hydrogel microsphere taking tremella polysaccharide as a substrate is helpful for finding out new properties of tremella polysaccharide and further expanding the application range of tremella polysaccharide. Microfluidic droplet technology can accurately manipulate fluids at the micrometer scale, and the preparation of highly monodisperse droplet templates is considered as an ideal means for preparing uniform microspheres. Based on different gel curing principles, physical crosslinking, chemical crosslinking, enzyme-induced crosslinking and photo-crosslinking, different types of hydrogel microspheres have been developed and prepared by utilizing microfluidic technology. However, in view of the special physicochemical properties of tremella polysaccharide, there is no successful case of combining the precise forming capability of microfluidic emulsion with a gentle in-situ crosslinking mechanism suitable for tremella polysaccharide. Specifically, how to design a reaction system, so that small molecules (such as acid) in an outer phase can diffuse through an oil phase intermediate layer, trigger tremella polysaccharide in an inner water phase to perform a rapid crosslinking reaction with a specific crosslinking agent, and thus, the preparation of uniformity is realized, and the technical problem to be solved in the field is still needed. Disclosure of Invention In order to solve the problems, the invention provides a tremella polysaccharide-based hydrogel microsphere, and a preparation method and application thereof. The invention provides a method for preparing tremella polysaccharide-based hydrogel microspheres, which comprises the following steps: (1) Preparing a water phase containing tremella polysaccharide and cationic polymer; (2) Preparing an oil phase containing a surfactant; (3) Preparing water-in-oil droplets through a capillary glass tube single-stage microfluidic device; (4) Introducing the oil phase containing the water-in-oil droplets into a receiving system for crosslinking to obtain the tremella polysaccharide-based hydrogel microsphere, wherein the receiving system is the oil phase containing acetic acid. Preferably, the method comprises the steps of, In the step (1), the preparation method of the water phase comprises the following steps: (a) Preparing tremella polysaccharide aqueous solution with mass fraction of 0.05-1.5wt%; (b) Preparing a cationic polymer aqueous solution with the mass fraction of 0.1-4.0wt%; (c) Mixing tremella polysaccharide aqueous solution and cationic polymer aqueous solution according to the volume ratio of (1-5): 1, and regulating the pH value to 5.7-6.4 to obtain a water phase; And/or, in the step (2), the preparation method of the oil phase comprises the following steps: Adding a surfactant into a continuous phase, and uniformly mixing to obtain an oil phase with the mass fraction of 0.5-10.0wt% of the surfactant, wherein the continuous phase is selected from soybean oil, mineral oil, liquid paraffin, silicone oil or fluorine oil, and the surfactant is selected from polyglycerol polyricinoleate, span80 or special surfactant for fluorine oil. Preferably, the method comprises the steps of, In the step (a), the mass fraction of the tremella polysaccharide water solution is 1.0wt%; and/or in the step (b), the mass fraction of the cationic polymer aqueous solution is 2.0-4.0wt%; And/or, in the step (c), the volume ratio of the tremella polysaccharide water solution to the cationic polymer water solution is 3:1; and/or, in step (c), said adjusting the pH to 5.9; and/or the mass fraction of surfactant in the oil phase is 5.0 wt%; and/or, the continuous phase is selected from soybean oil; and/or the surfactant is selected from polyglycerol polyricinoleate. Preferably, the method comprises the steps of, In step (b), the cationic polymer is chitosan; and/or, in step (c), the pH is adjusted using an aqueous sodium hydroxide solution having a concentration of 1.0 wt%. Preferably, the method comprises the steps of, In the step (3), when water-in-oil droplets are prepared, the flow rate of the water phase is 10-1500 mu L/h, and the flow rate of the oil phase is 200-10000 mu L/h; And/or in the step (4), the volume fraction of acetic acid in the oil phase containing acetic acid is 0.05-0.5 v/v%; and/or, in the step (4), the cro