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CN-122011787-A - Preparation method of high-strength antibacterial silk fibroin contact lens

CN122011787ACN 122011787 ACN122011787 ACN 122011787ACN-122011787-A

Abstract

The invention belongs to the technical field of cornea contact lenses, and particularly discloses a preparation method of a high-strength antibacterial silk fibroin contact lens. The silk fibroin preparation method comprises the steps of degumming silkworm silk, dissolving a lithium bromide solution to obtain a silk fibroin solution, mixing the silk fibroin solution with a chitosan nanofiber solution and quaternized chitosan nanofibers to obtain a silk fibroin-chitosan nanofiber-quaternized chitosan nanofiber mixed solution, mixing the silk fibroin-chitosan nanofiber-quaternized chitosan nanofiber mixed solution with an acrylamide solution to obtain a quaternary blending solution, transferring the quaternary blending solution into a contact lens mold, and carrying out gradient air drying treatment to obtain the silk fibroin contact lens. The high-strength antibacterial silk fibroin contact lens prepared by the invention has excellent mechanical property and durability, and also has antibacterial property.

Inventors

  • TANG YUXI
  • Cui Huanrui
  • ZHANG DEBO

Assignees

  • 甘肃康视丽隐形眼镜有限公司

Dates

Publication Date
20260512
Application Date
20260408

Claims (9)

  1. 1. A method for preparing a high-strength antibacterial silk fibroin contact lens, which is characterized by comprising the following steps: 1) Degumming silkworm silk, and then dissolving the silkworm silk by using a lithium bromide solution to obtain a silk fibroin solution; 2) Mixing the silk fibroin solution with a chitosan nanofiber solution and quaternized chitosan nanofibers to obtain a silk fibroin-chitosan nanofiber-quaternized chitosan nanofiber mixed solution; 3) Mixing a silk fibroin-chitosan nanofiber-quaternized chitosan nanofiber mixed solution with an acrylamide solution to obtain a quaternary blending solution; 4) Transferring the obtained quaternary blend liquid into a contact lens mould, and carrying out gradient air drying treatment to obtain the silk fibroin contact lens.
  2. 2. The method for preparing a high-strength antimicrobial silk fibroin contact lens according to claim 1, wherein the degumming treatment in the step 1) comprises the steps of placing silk of bombyx mori in sodium carbonate solution for degumming treatment; The pH value of the sodium carbonate solution is 10-11; the degumming treatment is carried out under the boiling condition, and the degumming treatment time is 25-35 min.
  3. 3. The method for preparing a high-strength antimicrobial silk fibroin contact lens according to claim 2, wherein the molar concentration of the lithium bromide solution in the step 1) is 9.3mol/L; The mass concentration of the silk fibroin solution is 5-10%.
  4. 4. The method for preparing a high-strength antibacterial silk fibroin contact lens according to any one of claims 1-3, wherein the mass ratio of the sum of chitosan nanofibers and quaternized chitosan nanofibers in the chitosan nanofiber solution and quaternized chitosan nanofibers in the step 2) to silk fibroin in the silk fibroin solution is 1-3:100; the mass ratio of the chitosan nanofiber to the quaternized chitosan nanofiber is 60-80:20-40; The mass concentration of the chitosan nanofiber solution and the quaternized chitosan nanofiber solution is independently 0.45-3%; Wherein the diameter of the chitosan nanofiber and the quaternized chitosan nanofiber are independently 10-50 nm, and the length of the chitosan nanofiber and the quaternized chitosan nanofiber are independently 0.5-3 mu m.
  5. 5. The method for preparing a high-strength antibacterial silk fibroin contact lens according to claim 4, wherein in the step 2), the pH value of the mixture is 5.5-6.5, the mixing rotation speed is 300-500 rpm, and the mixing time is 10-24 h.
  6. 6. The method for preparing a high-strength antibacterial silk fibroin contact lens according to claim 5, wherein the mass ratio of the solute of the silk fibroin-chitosan nanofiber-quaternized chitosan nanofiber mixed solution to the acrylamide in the acrylamide solution in the step 3) is 100:15-30; The rotating speed of the mixing is 300-500 rpm, and the mixing time is 40-80 min.
  7. 7. The method for producing a high-strength antimicrobial silk fibroin contact lens according to claim 5 or 6, wherein the gradient air-drying treatment comprises a high-humidity air-drying treatment, a low-humidity air-drying treatment and a low-humidity air-drying treatment which are sequentially performed.
  8. 8. The method for preparing a high-strength antibacterial silk fibroin contact lens according to claim 7, wherein the temperature of Gao Shifeng in dry treatment is room temperature, the relative humidity is 70-75%, and the time is 6-12 h; the temperature of the dehumidification air-drying treatment is 25-30 ℃, the relative humidity is linearly reduced, the relative humidity reduction end point is 35-40%, and the time is 6-7 h; The low-humidity air drying treatment is carried out under a drying condition, the temperature is 25-30 ℃, and the time is 12-48 h.
  9. 9. The high-strength antibacterial silk fibroin contact lens prepared by the preparation method of any one of claims 1-8.

Description

Preparation method of high-strength antibacterial silk fibroin contact lens Technical Field The invention relates to the technical field of cornea contact lenses, in particular to a preparation method of a high-strength antibacterial silk fibroin contact lens. Background In the technological development process of contact lens materials, silk fibroin has become an important biological material direction for replacing traditional synthetic high polymer materials (such as polymethyl methacrylate, silicon hydrogel and the like) due to excellent biocompatibility, optical transparency and renewable characteristics of natural silk, and related preparation schemes are disclosed. In the prior art, pure silk fibroin solution is generally adopted to prepare the contact lens by means of casting, molding or rotary pouring and the like, and although the method can realize basic optical functions and biosafety, the core defect is that the inherent mechanical property of the material is insufficient, so that the practicability and durability of the product are severely restricted. Specifically, the gel or solid film network structure formed by pure silk fibroin is relatively homogeneous and lacks an effective reinforcing phase, so that key mechanical indexes such as tensile strength, elongation at break and elastic modulus of the lens are often low, microcracks, plastic deformation and even tearing are easily generated due to daily wearing, rubbing or cleaning operation in actual use, and the dimensional stability and structural integrity of the lens are difficult to maintain for a long time. In addition, mechanical property weakness also limits the possibility of lens design, can not ensure ultra-thin and high oxygen permeability and simultaneously has enough mechanical toughness, thereby affecting wearing comfort and safety. The prior published proposal tries to optimize by changing the concentration of silk fibroin, the type of cross-linking agent or the parameters of the processing technology, but the adjustment has limited improvement range of mechanical properties, and can not fundamentally solve the problem of insufficient toughness of the material at the expense of transparency, oxygen permeability or biocompatibility. In addition, when the contact lens is worn for a long time, microorganisms are easy to grow on the surface of the lens, and particularly bacteria such as staphylococcus aureus, escherichia coli and the like are easy to adhere and form a biological film which is difficult to remove under the combined action of tears, protein deposition and environmental microorganisms. Although silk fibroin itself has a certain antimicrobial potential, it does not meet the antimicrobial requirements of contact lenses. Therefore, how to provide a preparation method of a high-strength antibacterial silk fibroin contact lens, which improves the durability and mechanical strength of the silk fibroin contact lens and simultaneously endows the silk fibroin contact lens with excellent antibacterial performance is a problem to be solved in the field. Disclosure of Invention In view of the above, the invention provides a preparation method of a high-strength antibacterial silk fibroin contact lens, so as to solve the problems of poor mechanical properties and low high-strength antibacterial property of the traditional silk fibroin contact lens. In order to achieve the above purpose, the invention adopts the following technical scheme: a preparation method of a high-strength antibacterial silk fibroin contact lens comprises the following steps: 1) Degumming silkworm silk, and then dissolving the silkworm silk by using a lithium bromide solution to obtain a silk fibroin solution; 2) Mixing the silk fibroin solution with a chitosan nanofiber solution and quaternized chitosan nanofibers to obtain a silk fibroin-chitosan nanofiber-quaternized chitosan nanofiber mixed solution; 3) Mixing a silk fibroin-chitosan nanofiber-quaternized chitosan nanofiber mixed solution with an acrylamide solution to obtain a quaternary blending solution; 4) Transferring the obtained quaternary blend liquid into a contact lens mould, and carrying out gradient air drying treatment to obtain the silk fibroin contact lens. Preferably, the degumming treatment in the step 1) comprises the steps of placing silkworm silk in a sodium carbonate solution for degumming treatment; The pH value of the sodium carbonate solution is 10-11; the degumming treatment is carried out under the boiling condition, and the degumming treatment time is 25-35 min. Preferably, the molar concentration of the lithium bromide solution in step 1) is 9.3mol/L; The mass concentration of the silk fibroin solution is 5-10%. Preferably, in the step 2), the mass ratio of the sum of the chitosan nanofibers and the quaternized chitosan nanofibers in the chitosan nanofiber solution and the quaternized chitosan nanofiber solution to the silk fibroin in the silk fibroin solution is 1-3:100; the