CN-122011842-A - Preparation method and application of sprayable surface-adaptive packaging film for in-situ detection of organophosphorus pesticide

Abstract

The invention relates to a preparation method and application of a sprayable surface adaptive packaging film for in-situ detection of organophosphorus pesticides, wherein carboxylated cellulose nanofiber mediated defect type UiO-66-OH is used as a core functional material, a sprayable slurry is prepared by matching with an aqueous film forming matrix, and a continuous compact film layer is formed through a synchronous spraying process. The packaging film obtained by the method can provide stable and independent reaction and fluorescence response environments for in-situ detection and preliminary degradation of organophosphorus pesticides, realize detection-degradation-protection integrated functions, overcome the problems of poor in-situ laminating property, single function, complex operation and easy pollution in the prior art, realize single-step spray forming and one-paper completion of multiple functions, is suitable for detection of organophosphorus pesticide residues on the surfaces of various fruits and vegetables, and is environment-friendly, high in stability, easy to store and low in cost.

Inventors

• DOU XINCUN
• LIANG XINRU
• LIU YALI
• Zu Baidai

Assignees

• 中国科学院新疆理化技术研究所

Dates

Publication Date

20260512

Application Date

20260310

Claims (2)

1. 1. The preparation method of the sprayable surface-adaptive packaging film for in-situ detection of the organophosphorus pesticide is characterized in that the packaging film is prepared from defective UiO-66-OH, sodium alginate and calcium chloride solution, and the specific operation is carried out according to the following steps: preparation of an aqueous defective UiO-66-OH solution: a. dissolving carboxylated cellulose in N, N-dimethylformamide, and fully stirring at room temperature until the carboxylated cellulose is completely dissolved to obtain a mixture; b. zirconium chloride was dissolved in 1.5 mL of n, n-dimethylformamide to obtain a mixture; c. Adding the mixture obtained in the step b into the carboxylated nanocellulose dispersion liquid obtained in the step a, and stirring for 4 h to obtain a mixture solution; d. dissolving 2-hydroxy terephthalic acid into 1.5 mL of N, N-dimethylformamide solution, and stirring until the solution is completely dissolved to obtain ligand solution; f. adding the ligand solution obtained in the step d into the mixture solution obtained in the step c, and fully stirring 1h until the mixture solution is uniform and has no layering; g. Adding 6.4 mL of acetic acid and 0.25 of mL of deionized water into the solution obtained in the step f, and stirring for 10 min to obtain a mixed solution; h. Transferring the mixed solution obtained in the step g into a polyvinyl fluoride reaction kettle, reacting for 1 hour in an oven preheated to 120 ℃, transferring the solution into a centrifuge tube after the reaction is finished, putting the centrifuge tube into a reaction kettle liner, heating to 100-150 ℃, reacting for 1-3 h ℃, cooling and standing after the reaction is finished, and repeatedly centrifuging and washing for 3 times by using N, N-dimethylformamide and pure water until no fluorescence exists, thus obtaining a defective UiO-66-OH aqueous solution; preparation of defective UiO-66-OH/sodium alginate: i, slowly pouring sodium alginate into the stirring pure water, stirring for at least 24 hours until the sodium alginate is uniformly dispersed, then slowly adding the sodium alginate into the defect type UiO-66-OH aqueous solution obtained in the step h, heating to 50-80 ℃, reacting for 1-2 hours, and cooling to room temperature after the reaction is finished to obtain the defect type UiO-66-OH/sodium alginate; j. Preparing a calcium chloride aqueous solution with the concentration of 0.1 mg/mL: k. Spraying the defect type UiO-66-OH/sodium alginate obtained in the step i and the calcium chloride aqueous solution obtained in the step j on a die, and self-assembling at room temperature to obtain a transparent packaging film; Or directly spraying the defect type UiO-66-OH/sodium alginate obtained in the step i and the calcium chloride aqueous solution obtained in the step j on fruits and vegetables, and forming a film to form a transparent packaging film by self-assembly.
2. 2. Use of a packaging film obtained according to the method of claim 1 for the detection of the organophosphorus pesticide glyphosate, dichlorvos or dimethoate.

Description

Preparation method and application of sprayable surface-adaptive packaging film for in-situ detection of organophosphorus pesticide Technical Field The invention relates to the field of sprayable packaging film processing technology and pesticide in-situ detection, in particular to a preparation method and application of a sprayable surface-adaptive packaging film for in-situ detection of organophosphorus pesticide. Background The organophosphorus pesticide is widely applied to the fruit and vegetable planting process due to the high-efficiency insecticidal and bactericidal properties, but residues are easy to accumulate through a food chain, and potential hazards are caused to the nervous system and the endocrine system of a human body, so that the in-situ rapid detection of the organophosphorus pesticide on the surface of the fruit and vegetable is realized, and the method has important significance for guaranteeing the food safety. The existing organophosphorus pesticide detection method mainly comprises a gas chromatography method, a liquid chromatography-mass spectrometry method, an enzyme inhibition method and the like, wherein the detection accuracy of the chromatography method is high, professional equipment, tedious pretreatment and professional operators are needed, the on-site in-situ rapid detection requirement cannot be met, the enzyme inhibition method is simple and convenient to operate, the enzyme activity is easily influenced by temperature and humidity, the stability is poor, and the detection specificity is insufficient. The fluorescence sensing technology has been attracting attention in the field of in-situ detection by virtue of the advantages of rapid response, high sensitivity, strong visualization and the like. Zirconium-based metal organic frameworks (UiO-66-OH) are used as typical metal organic frameworks (metal-organic frameworks, MOFs) materials, have controllable pore structures, excellent optical properties and zirconium coordination vacancy active sites, can realize specific identification of organophosphorus pesticides through defect engineering regulation and control of fluorescence characteristics, have good self-assembly performance, biocompatibility and green renewable characteristics, can be used as dispersion media and film-forming auxiliaries, and improve stability and film-forming property of the metal organic framework materials. The two materials are compounded to prepare the sprayable film material, so that the fluorescence sensing advantage of the metal organic framework can be exerted, the film forming performance of carboxylated Cellulose Nanofibers (CNFs) can be used for adapting to the complex surfaces of fruits and vegetables, packaging protection and in-situ detection are integrated, and the sprayable film material becomes a current research hot spot. The sprayable packaging film has huge application potential in the field of food preservation and functional packaging due to the characteristics of convenient construction, strong surface adaptability (being capable of being attached to concave-convex surfaces of fruits and vegetables), no need of complex forming equipment and the like. The existing sprayable film focuses on the fresh-keeping and blocking functions, and the technology of combining the fluorescence sensing function with the sprayable packaging film is still in an exploration stage. The existing fluorescent sensing films are prepared by dip coating and knife coating processes, the uniformity of the film thickness is poor, the film is difficult to adapt to complex surfaces such as curved surfaces and folds of fruits and vegetables, meanwhile, most of the sensing films only have a single detection function, packaging protection performance cannot be considered, and the sensing materials are easy to fall off and insufficient in stability, so that the requirements of actual handheld operation and long-term storage are difficult to meet. To achieve fusion of fluorescence sensing and sprayable packaging functions, the prior art proposes various improvements, such as: CN 114544574A discloses a method for detecting organophosphorus pesticides by using a microfluidic chip based on a fluorescence sensing film, wherein a porous fluorescence sensing film is obtained by constructing a platinum nanoparticle@oxalate-organic metal frame composite material and a porous two-dimensional nano sheet with the functions of specifically detecting organophosphorus pesticides and blocking macromolecular interferents through layer-by-layer self-assembly, and the microfluidic chip is formed by a sample channel, a sample introduction channel, a reaction tank, a microfluidic channel, a detection tank and an optical fiber channel. Then, the organophosphorus pesticide detection system is built by combining the portable constant-pressure injection pump, the laser, the spectrometer, the signal transmitter and the signal display, so that the detection equipment is miniaturized a