CN-122012088-A - Carbon quantum dot, preparation method, application of carbon quantum dot in flavonoid detection and flavonoid concentration detection method

Abstract

The invention provides a carbon quantum dot, a preparation method, application of the carbon quantum dot in flavonoid compound detection and a flavonoid compound concentration detection method. The carbon quantum dot has two excitation wavelengths of 313nm and 374nm. The method comprises the steps of irradiating the carbon quantum dots with 313nm excitation light, wherein the intensity of 420nm fluorescence emitted by the carbon quantum dots is I 313 , and irradiating the carbon quantum dots with 374nm excitation light, wherein the intensity of 420nm fluorescence emitted by the carbon quantum dots is I 374 . The flavonoid compound can cause the carbon quantum dot to generate fluorescence quenching, and the reduction speeds of I 313 and I 374 are different along with the increase of the concentration of the flavonoid compound, so that the concentration of the flavonoid compound can be detected by using I 313 /I 374 .

Inventors

• PENG SITING
• CHEN DA
• ZHANG YIFAN
• WANG SIHAO
• XIAO WENDI
• GAO LIWEI
• SHI JUNYUAN

Assignees

• 宁波大学

Dates

Publication Date

20260512

Application Date

20260202

Claims (10)

1. 1. A preparation method of carbon quantum dots is characterized in that 5-amino isophthalic acid and catechol are dissolved in a solvent, and then the solvent is heated in a reaction kettle for reaction, so that the carbon quantum dots are obtained.
2. 2. The method for preparing carbon quantum dots according to claim 1, wherein the mass ratio of 5-aminoisophthalic acid to catechol is 1:1.
3. 3. The method for preparing carbon quantum dots according to claim 1, wherein the reaction temperature of 5-aminoisophthalic acid and catechol is 180 ℃.
4. 4. The method for preparing carbon quantum dots according to claim 1, wherein the reaction time of 5-aminoisophthalic acid and catechol is 8 hours.
5. 5. The method for preparing carbon quantum dots according to claim 1, wherein the solvent is ethanol.
6. 6. A carbon quantum dot, which is prepared by the preparation method of the carbon quantum dot according to any one of claims 1 to 5.
7. 7. The use of the carbon quantum dot according to claim 6 in detection of flavonoids.
8. 8. The method for detecting the concentration of the flavonoid compound is characterized by comprising the following steps of: irradiating the carbon quantum dot according to claim 6 with 313nm excitation light to obtain a fluorescence intensity of i 313 at 420nm emitted by the carbon quantum dot; Irradiating the carbon quantum dot according to claim 6 with 374nm excitation light to obtain a fluorescence intensity of i 374 at 420nm emitted by the carbon quantum dot; Mixing the carbon quantum dot according to claim 6 with a liquid to be measured to form a mixed liquid; Irradiating the mixed solution with 313nm excitation light to obtain 420nm fluorescence intensity I 313 emitted by the carbon quantum dots; irradiating the mixed solution with 374nm excitation light to obtain 420nm fluorescence intensity I 374 emitted by the carbon quantum dots; And determining the concentration of the flavonoid compound in the liquid to be tested based on I 313 /I 374 and I 313 /i 374 .
9. Use of hca in the detection of flavonoids, characterized by PLE spectra based on carbon quantum dots according to claim 6.
10. Use of lda in the detection of flavonoids, characterized by PLE spectra based on carbon quantum dots as claimed in claim 6.

Description

Carbon quantum dot, preparation method, application of carbon quantum dot in flavonoid detection and flavonoid concentration detection method Technical Field The invention relates to the field of flavonoid compound detection, in particular to a carbon quantum dot, a preparation method, application in flavonoid compound detection and a flavonoid compound concentration detection method. Background Flavonoids are a class of polyphenolic compounds that have wide application in the fields of food science, medicine and cosmetics. Among the flavonoids, quercetin, rutin, morin and kaempferol are known for their anti-inflammatory properties, and have great potential in the prevention and treatment of various chronic diseases. In particular, these compounds have been shown to have therapeutic effects on cardiovascular diseases and to be able to prevent hypertension, protect the liver, reduce the incidence of coronary heart disease. In addition, the flavonoid compound has strong free radical scavenging capability, can induce apoptosis of tumor cells, and delay the apoptosis process of normal tissue cells. However, excessive intake of flavonoids may cause gastrointestinal irritation and liver dysfunction, and disturb hormone metabolism, triggering endocrine disorders. Further studies have shown that long-term large-dose intake of flavonoids may overload the physiological system of the body, thereby triggering Reactive Oxygen Species (ROS) production, ultimately leading to DNA damage. In addition, the pharmacological actions of the flavonoid compounds of different types are obviously different, and the mixture of the flavonoid compounds can generate more serious adverse reactions than a single component. Therefore, the identification of the concentration and the type of the flavonoid compounds is of great significance. Disclosure of Invention Based on the above, it is necessary to provide a carbon quantum dot, a preparation method, an application in flavonoid detection and a method for detecting the concentration of flavonoid in order to solve the problem that effective concentration identification and component identification cannot be performed on flavonoid in the prior art. The technical scheme provided by the invention is as follows: A preparation method of carbon quantum dots comprises the steps of dissolving 5-amino isophthalic acid and catechol in a solvent, and then heating and reacting in a reaction kettle to obtain the carbon quantum dots. In some embodiments of the application, the mass ratio of 5-aminoisophthalic acid to catechol is 1:1. In some embodiments of the application, the reaction temperature of 5-aminoisophthalic acid and catechol is 180 ℃. In some embodiments of the application, the reaction time of 5-aminoisophthalic acid and catechol is 8 hours. In some embodiments of the application, the solvent is ethanol. The carbon quantum dot is prepared by adopting the preparation method of the carbon quantum dot. An application of the carbon quantum dot in flavonoid detection. A concentration detection method of flavonoid compounds comprises the following steps: Irradiating the carbon quantum dots with 313nm excitation light to obtain the fluorescence intensity of 420nm emitted by the carbon quantum dots as i 313; Irradiating the carbon quantum dots with 374nm excitation light to obtain the fluorescence intensity of 420nm emitted by the carbon quantum dots as i 374; mixing the carbon quantum dots with a liquid to be detected to form a mixed liquid; Irradiating the mixed solution with 313nm excitation light to obtain 420nm fluorescence intensity I 313 emitted by the carbon quantum dots; irradiating the mixed solution with 374nm excitation light to obtain 420nm fluorescence intensity I 374 emitted by the carbon quantum dots; And determining the concentration of the flavonoid compound in the liquid to be tested based on I 313/I374 and I 313/i374. The application of HCA in flavonoid detection is based on PLE spectrum of the carbon quantum dot. Application of LDA in detection of flavonoid compounds is based on PLE spectrum of the carbon quantum dots. The beneficial effects of the invention are as follows: The carbon quantum dot has two excitation wavelengths of 313nm and 374nm. The method comprises the steps of irradiating the carbon quantum dots with 313nm excitation light, wherein the intensity of 420nm fluorescence emitted by the carbon quantum dots is I 313, and irradiating the carbon quantum dots with 374nm excitation light, wherein the intensity of 420nm fluorescence emitted by the carbon quantum dots is I 374. The flavonoid compound can cause the carbon quantum dot to generate fluorescence quenching, and the reduction speeds of I 313 and I 374 are different along with the increase of the concentration of the flavonoid compound, so that the concentration of the flavonoid compound can be detected by using I 313/I374. Secondly, after the carbon quantum dots and the flavonoid compounds are mixed, the fluore