CN-122012218-A - Cell climbing piece fixing device of adaptation cell culture board

Abstract

The invention provides a cell climbing sheet fixing device adapting to a cell culture plate, and relates to the field of auxiliary equipment for cell experiments. The invention provides a cell climbing sheet fixing device for adapting to a cell culture plate, which comprises a positioning base, wherein the positioning base is provided with a placing groove, the bottom of the placing groove is provided with a central limiting groove, the inside of the central limiting groove is provided with a plurality of clamping jaws, one end of each clamping jaw is connected with the inner wall of the central limiting groove, the other end of each clamping jaw is used as a free end, the clamping jaw is provided with an inclined guide surface, the clamping jaw can elastically deform or elastically displace, and the free end of each clamping jaw can prop against the cell climbing sheet. According to the invention, through an integrated structural design, the problems of non-centering, difficult taking, easy breaking and poor suitability in the cell climbing plate hole are synchronously solved, and the stability, convenience and experiment success rate of cell climbing plate culture are improved.

Inventors

• HONG WEIFENG
• ZHU ZICHUN
• WEI WEI
• ZHU JI

Assignees

• 浙江省肿瘤医院

Dates

Publication Date

20260512

Application Date

20260210

Claims (10)

1. 1. A cell climbing sheet fixing device suitable for a cell culture plate is characterized by comprising a positioning base, wherein the positioning base is provided with a placing groove, the bottom of the placing groove is provided with a central limiting groove, a plurality of clamping jaws are arranged in the central limiting groove, one ends of the clamping jaws are connected with the inner wall of the central limiting groove, the other ends of the clamping jaws are used as free ends, the clamping jaws are provided with inclined guide surfaces, the clamping jaws can elastically deform or elastically displace, and the free ends of the clamping jaws can support against the cell climbing sheet.
2. 2. The cell climbing sheet fixing device for adapting to a cell culture plate according to claim 1, wherein a plurality of clamping grooves are formed in the outer wall of the positioning base, the tops of the clamping grooves are opened, and at least two clamping grooves are arranged oppositely.
3. 3. The cell climbing sheet fixing device according to claim 2, wherein the top of the positioning base is further provided with an outwardly extending hanging portion.
4. 4. The cell climbing sheet fixing device according to claim 3, wherein the hanging portion is provided with a notch, and the notch is communicated with the clamping groove.
5. 5. The cell slide fastener adapted to a cell culture plate according to claim 1, wherein the free ends of the clamping jaws are curved.
6. 6. The device for fixing a cell climbing sheet adapted to a cell culture plate according to claim 1, wherein the inclined guide surface is inclined downward in a direction toward the free end of the clamping jaw, and has a gradient of 30-60 ̊.
7. 7. The cell slide fastener adapted to a cell culture plate according to claim 1, wherein each of the clamping jaws is disposed uniformly around the placement groove.
8. 8. The device for fixing a cell climbing plate according to claim 1, wherein the diameter of an inscribed circle formed by connecting the free ends of the clamping jaws is 0.5-2 mm smaller than the outer diameter of the climbing plate.
9. 9. The cell climbing sheet fixing device for adapting to the cell culture plate according to claim 1, wherein the depth of the central limiting groove is 1/3-1/2 of the thickness of the cell climbing sheet, the height of the clamping jaw is consistent with the depth of the central limiting groove, and the outer diameter of the positioning base is 0.1-0.2 mm smaller than the outer diameter of a hole of the cell culture plate.
10. 10. The cell climbing sheet fixing device according to claim 1, wherein the material of the clamping jaw is any one of liquid silica gel, polyether ether ketone and high-density polyethylene, and/or the material of the positioning base is polycarbonate.

Description

Cell climbing piece fixing device of adaptation cell culture board Technical Field The invention relates to the field of auxiliary equipment for cell experiments, in particular to a cell climbing sheet fixing device adapting to a cell culture plate. Background In the cell slide experiment, the stable fixation of the slide is a key precondition for ensuring the success of the experiment. In the prior art, the experimenter mostly puts the climbing sheet into the culture pore plate directly, relies on the surface tension of the culture medium or slightly presses the tweezers to realize 'fixation', but the mode has obvious defects: 1. the climbing piece location is inaccurate, and traditional mode is many with the climbing piece direct placement cell culture board etc. standard culture dish downthehole, lacks special location structure, leads to the climbing piece easy skew, not centering. The method not only affects the uniform adhesion of cells after inoculation, but also causes dislocation of an observation area due to the position deviation of the climbing piece during subsequent dyeing and microscopic examination, and reduces the reliability of experimental data; 2. The traditional device only realizes basic bearing function, lacks a pre-positioning and elastic clamping structure for the climbing slices, and cannot meet the experimental requirements of 'accurate fixing' and 'convenient operation' at the same time; 3. The climbing sheet is easy to damage when being taken and put, and is usually made of thin glass material and has fragile texture. In the existing operation, experimenters need to directly clamp the climbing slices in the holes by using tweezers, clamping force is difficult to control, and edge cracking and surface scratch of the climbing slices are easy to cause. Some existing climbing sheet fixing brackets mostly adopt metal clamping or clamping groove structures, but have complex structures, are difficult to clean and sterilize and are difficult to adapt to standard pore plates although a certain fixing effect can be provided. Therefore, the development of the cell climbing sheet adapting device with the functions of accurate positioning, stable fixing and convenient taking becomes a key technical direction for improving the experimental efficiency and reliability of cell culture. Disclosure of Invention The invention aims to overcome the defects of the prior art, and provides a cell climbing sheet fixing and culturing device which is designed to synchronously solve the problems of non-centering, difficult access, easy breakage and poor suitability of climbing sheet holes through an integrated structure, thereby improving the stability, convenience and experiment success rate of cell climbing sheet culture. The cell climbing sheet fixing device suitable for the cell culture plate comprises a positioning base, wherein the positioning base is provided with a placing groove, the bottom of the placing groove is provided with a central limiting groove, the inside of the central limiting groove is provided with a plurality of clamping jaws, one ends of the clamping jaws are connected with the inner wall of the central limiting groove, the other ends of the clamping jaws are used as free ends, the clamping jaws are provided with inclined guide surfaces, the clamping jaws can elastically deform or elastically displace, and the free ends of the clamping jaws can support against the cell climbing sheet. In a possible implementation manner, the outer wall of the positioning base is provided with a plurality of clamping grooves, the tops of the clamping grooves are opened, and at least two clamping grooves are arranged oppositely. In a possible embodiment, the top of the positioning base is further provided with an outwardly extending hooking portion. In a possible embodiment, the hooking portion is provided with a notch, and the notch is communicated with the clamping groove. In a possible embodiment, the free ends of the clamping jaws are curved. In a possible implementation manner, the inclined guide surface is inclined downwards along the direction towards the free end of the clamping jaw, and the gradient is 30-60 ̊. In a possible embodiment, each of the clamping jaws is arranged uniformly around the placement groove. In a possible implementation manner, the diameter of an inscribed circle formed by connecting the free ends of the clamping jaws is 0.5-2 mm smaller than the outer diameter of the climbing sheet. In a feasible implementation mode, the depth of the central limiting groove is 1/3-1/2 of the thickness of the cell climbing plate, the height of the clamping jaw is consistent with the depth of the central limiting groove, and the outer diameter of the positioning base is 0.1-0.2 mm smaller than the outer diameter of a hole of the cell culture plate. In a possible embodiment, the material of the clamping jaw is selected from any one of liquid silica gel, polyetheretherketone and high densit