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CN-122012219-A - High-throughput breeding device for tetramethylpyrazine strain

CN122012219ACN 122012219 ACN122012219 ACN 122012219ACN-122012219-A

Abstract

The invention relates to the technical field of tetramethylpyrazine strain breeding, in particular to a tetramethylpyrazine strain high-throughput breeding device. The novel solar energy breeding box comprises a breeding box body, wherein a door plate is rotatably arranged on the breeding box body, a handle is fixedly connected to the side wall of the door plate, a magnetic strip is embedded in the upper portion of the breeding box body, a control panel is arranged on the side wall of the breeding box body, ultraviolet lamps which are symmetrically distributed are arranged on the side wall of the breeding box body, a mounting frame is arranged at the bottom of the breeding box body, and a mounting groove is formed in the mounting frame. According to the invention, the addition amount of nitrosoguanidine is accurately controlled through the quantitative assembly, the concentration and the dosage of the mutagen fed in each adding tank are consistent, the problem that the addition amount of nitrosoguanidine is difficult to control in a standardized way in traditional chemical mutagenesis is solved, the difference of the mutagenesis effects of strains in different batches is effectively avoided, the stability of the positive mutation rate of the strains is ensured, and the reliability of the chemical mutagenesis is improved.

Inventors

  • HAO BIAO
  • ZHANG JIE
  • YANG HONGWEN
  • Pan tianquan
  • LI NA
  • DAI SEN
  • WANG YUE

Assignees

  • 安徽金种子酒业股份有限公司

Dates

Publication Date
20260512
Application Date
20260302

Claims (10)

  1. 1. The utility model provides a tetramethyl pyrazine bacterial strain high-throughput breeding device, its characterized in that, including breeding case (1), rotate on breeding case (1) and install door plant (2), the lateral wall rigid coupling of door plant (2) has handle (3), the upper portion of breeding case (1) is inlayed and is equipped with magnetic stripe (4), control panel (5) are installed to the lateral wall of breeding case (1), ultraviolet lamp (6) that are symmetrical distribution are installed to the lateral wall in breeding case (1), mounting bracket (7) are installed to the bottom in breeding case (1), mounting bracket (7) are last to offer mounting groove (8), install lead screw (9) in mounting groove (8), install on mounting bracket (7) with servo motor (10) that lead screw (9) are connected, threaded connection on lead screw (9) have with nut (11) of mounting groove (8) sliding connection, the bottom rigid coupling of nut (11) has mounting panel (12), be provided with on mounting panel (12) and be used for the quantitative subassembly that the material adds, breeding case (1) inner wall and be equipped with quantitative subassembly that are equipped with and are connected with in the sealing tank (15) and add quantitative subassembly (15) and are connected with equidistant each other, the top rigid coupling of piston board (16) has movable rod (17) that run through adding jar (15) upper end, is equidistant distribution movable rod (17) upper end between rigid coupling have holding down plate (18), ultraviolet lamp (6) servo motor (10) with quantitative subassembly all with control panel (5) electricity is connected.
  2. 2. The tetramethylpyrazine strain high-throughput breeding device as claimed in claim 1, wherein the upper part of the door plate (2) close to one side wall of the magnetic strip (4) is provided with a magnetic attraction surface, so that the door plate is convenient to be matched with the magnetic strip (4) to seal the breeding box (1).
  3. 3. The tetramethylpyrazine strain high-throughput breeding device according to claim 2, wherein a discharge port (151) is formed in the bottom of the adding tank (15), and a membrane flap (152) is arranged in the discharge port (151).
  4. 4. A tetramethylpyrazine strain high-throughput breeding device as claimed in claim 3, characterized in that the quantitative component further comprises an electric telescopic rod (13) arranged at the bottom of the mounting plate (12), a fixed plate (14) is arranged at the telescopic end of the electric telescopic rod (13), the equidistant adding tanks (15) are embedded on the fixed plate (14), the fixed plate (14) is embedded with symmetrically distributed spring telescopic rods (19), the upper ends of the spring telescopic rods (19) are fixedly connected with the lower pressure plate (18), one side of the mounting plate (12) is detachably connected with a storage tank (20), the bottom of the storage tank (20) is detachably connected with a pump body (21), a feeding pipe of the pump body (21) is detachably communicated with an outlet of the storage tank (20), a feeding pipe (22) is communicated with a feeding pipe (22), the bottom of the fixed plate (14) is communicated with a feeding pipe (24) which is equidistant, and the feeding pipe (24) is communicated with the adjacent connecting pipes (23).
  5. 5. The tetramethylpyrazine strain high-throughput breeding device as claimed in claim 4, wherein the triggering component comprises a fixing frame (26) fixedly connected to the inner rear wall of the breeding box (1) and distributed at equal intervals, a transmission rack (25) is mounted on the side wall of the fixing plate (14), a sliding groove (28) and a containing groove (27) are sequentially formed in the fixing frame (26) from top to bottom, a wedge plate (29) is connected in the sliding groove (28) in a sliding manner, a reset spring (30) is fixedly connected between the wedge plate (29) and the breeding box (1), rectangular grooves penetrating through from top to bottom are formed in the wedge plate (29), inclined grooves (31) are formed in the wedge plate (29) on two sides of the rectangular grooves, sliding rods (32) are connected between the inclined grooves (31) in a sliding manner, and transmission pieces fixedly connected with the sliding rods (32) are mounted on the side wall in the containing groove (27).
  6. 6. The tetramethylpyrazine strain high-throughput breeding device as claimed in claim 5, wherein the transmission member comprises a shaft lever (33) rotatably connected to the inner side wall of the containing groove (27), a transmission gear (34) matched with the transmission rack (25) is mounted on the shaft lever (33), a disc (35) is fixedly connected to the end portion of the shaft lever (33), a pin rod (36) is fixedly connected to the eccentric position of the side wall of the disc (35), a hollow frame (37) is sleeved on the outer side of the pin rod (36), a connecting rod (38) is fixedly connected to the top of the hollow frame (37), the connecting rod (38) is in sliding connection with the fixing frame (26), and the upper end of the connecting rod (38) is fixedly connected with the sliding rod (32).
  7. 7. The tetramethylpyrazine strain high-throughput breeding device as claimed in claim 6, wherein grooves (39) which are symmetrically distributed are formed in the bottom of the breeding box (1), swinging plates (40) are connected in the grooves (39) through rotating rods, torsion springs are wound on the rotating rods on two sides of the swinging plates (40), one ends of the torsion springs are fixedly connected with the swinging plates (40), the other ends of the torsion springs are fixedly connected with the grooves (39), and clamping grooves (41) are formed in the swinging plates (40).
  8. 8. The tetramethylpyrazine strain high-throughput breeding device as claimed in claim 7, wherein a slot (42) is formed in the bottom of the breeding box (1), a supporting plate (43) is placed in the slot (42), a sliding rod (44) penetrating the breeding box (1) is fixedly connected to the bottom of the supporting plate (43), a connecting disc (45) is fixedly connected to the lower end of the sliding rod (44), a supporting spring (46) is fixedly connected between the connecting disc (45) and the bottom of the breeding box (1), a straight rack (47) is fixedly connected to the connecting disc (45) through a supporting rod, a straight gear (49) matched with the straight rack (47) is mounted on the bottom of the breeding box (1) through a bracket, and an arc-shaped rack (48) matched with the straight gear (49) is fixedly connected to the side wall of the door plate (2).
  9. 9. A tetramethylpyrazine strain high throughput seed selection device as defined in claim 8 in which the arcuate rack (48) and the door panel (2) are rotatably connected to the seed selection box (1) on the same axis.
  10. 10. The high-throughput breeding device for tetramethylpyrazine strains as defined in claim 9, wherein said arc-shaped racks (48) are axially offset from said spur racks (47) and are engaged with spur gears (49).

Description

High-throughput breeding device for tetramethylpyrazine strain Technical Field The invention relates to the technical field of tetramethylpyrazine strain breeding, in particular to a tetramethylpyrazine strain high-throughput breeding device. Background Tetramethyl pyrazine is a nitrogenous heterocyclic compound with important pharmacological activity and wide application value, has good chemical stability and biological activity, and is widely applied to a plurality of fields such as cardiovascular and cerebrovascular drug intermediates, food essence, tobacco additives, feed additives, novel materials and the like. At present, high-yield tetramethylpyrazine strains are obtained through a strain mutation breeding mode, when the strains are subjected to mutation in the prior art, the corresponding strains are placed into a miniaturized culture pore plate, then the mutation of the tetramethylpyrazine strains is realized through ultraviolet irradiation or a chemical mutagen adding mode, in the process, the addition amount of nitrosoguanidine is difficult to realize standardized control during the chemical mutation, the mutation effects of different batches of strains are easy to be different, the stability of positive mutation rate is further influenced, and in addition, the position deviation is easy to occur when the miniaturized culture pore plate is placed, the chemical mutagen is unevenly added, and the mutation effect and the breeding accuracy are influenced. Disclosure of Invention In order to overcome the defects in the background technology, the invention aims to provide a high-throughput breeding device for tetramethylpyrazine strains. The high-flux breeding device for the tetramethylpyrazine strain comprises a breeding box, wherein a door plate is rotatably installed on the breeding box, a handle is fixedly connected to the side wall of the door plate, a magnetic stripe is embedded in the upper portion of the breeding box, a control panel is installed on the side wall of the breeding box, ultraviolet lamps which are symmetrically distributed are installed on the side wall of the breeding box, a mounting frame is installed at the bottom of the breeding box, a mounting groove is formed in the mounting frame, a lead screw is installed in the mounting groove, a servo motor connected with the lead screw is embedded in the mounting frame, a nut which is in sliding connection with the mounting groove is connected with the lead screw in a threaded mode, a mounting plate is fixedly connected to the bottom of the nut, a quantitative assembly for material addition is arranged on the mounting plate, a trigger assembly matched with the quantitative assembly is arranged on the inner wall of the breeding box, the quantitative assembly comprises an adding tank which is distributed at equal intervals, a piston plate is connected in a sealing sliding mode in the adding tank, a moving rod which penetrates through the upper end of the adding tank is fixedly connected with the piston plate, a lower pressing plate is fixedly connected with the upper end of the moving rod which is distributed at equal intervals, and the ultraviolet lamp and the quantitative assembly is connected with the quantitative assembly. As an improvement of the scheme, the upper part of the door plate, which is close to one side wall of the magnetic strip, is provided with a magnetic attraction surface, so that the door plate is convenient to be matched with the magnetic strip to seal the breeding box. As an improvement of the scheme, a discharge hole is formed in the bottom of the adding tank, and a membrane flap is arranged in the discharge hole. As the improvement of above-mentioned scheme, the ration subassembly still including install in the electric telescopic handle of mounting panel bottom, the fixed plate is installed to electric telescopic handle's flexible end, is equidistant distributed add jar inlays and locates on the fixed plate, it is symmetrical distribution's spring telescopic handle to inlay on the fixed plate, spring telescopic handle's upper end with the holding down plate rigid coupling, one side of mounting panel can be dismantled and be connected with the storage tank, the bottom of storage tank can be dismantled and be connected with the pump body, the inlet pipe of the pump body with the communication can be dismantled to the export of storage tank, the discharge end intercommunication of the pump body has the conveyer pipe, the conveyer pipe intercommunication has the connecting pipe, the bottom intercommunication of connecting pipe has the conveyer pipe that is equidistant distribution, the conveyer pipe is in charge of and adjacent add jar intercommunication. As the improvement of above-mentioned scheme, trigger the subassembly including the rigid coupling in select seed case incasement back wall and be the mount that the equidistance distributes, drive rack is installed to the lateral wall of fixed plate, from last dow