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CN-122012226-A - Injection device for injecting liquid into plant leaves and injection head thereof

CN122012226ACN 122012226 ACN122012226 ACN 122012226ACN-122012226-A

Abstract

The application relates to an injection device for injecting liquid into plant leaves and an injection head thereof, wherein the injection head comprises a barrel body, which comprises a first opening, a second opening and a liquid channel, wherein the first opening is arranged at a first end of the barrel body, the second opening is arranged at a second end of the barrel body, the liquid channel is arranged in the barrel body and communicated with the first opening and the second opening and used for containing the liquid to pass through, and one or more first protrusions are arranged at the second end of the barrel body and used for forming a damaged wound on the plant leaves, and the second end of the barrel body is suitable for being contacted with the plant leaves and forming a liquid space separated from the outside of the barrel body between the damaged opening and the second end of the barrel body by the plant leaves. The injection head of the application can not hurt operators, has good gene transfer effect and less damage to plants.

Inventors

  • LI RENHAN
  • CHEN XIANG
  • MA MINGQIAN

Assignees

  • 北京神笔东升科技有限公司

Dates

Publication Date
20260512
Application Date
20260212

Claims (15)

  1. 1. An injection head for injecting liquid into plant leaves, comprising: A cylinder body including a first opening arranged at a first end of the cylinder body, a second opening arranged at a second end of the cylinder body, and a liquid channel arranged in the cylinder body and communicating the first opening and the second opening for receiving liquid therethrough, and One or more first protrusions disposed at the second end of the barrel for forming a lesion on the plant leaf; wherein the second end of the barrel is adapted to contact the plant leaves and to form a liquid space with the plant leaves between the lesion and the second end of the barrel separate from the exterior of the barrel.
  2. 2. The injection head according to claim 1, wherein the pressure of the liquid space is greater than the pressure of the outside during the liquid injection.
  3. 3. The injection head of claim 1, wherein the second end of the barrel comprises an annular end surface adapted to abut the plant leaf when the first protrusion pierces the plant leaf, thereby defining a liquid space isolated from the environment.
  4. 4. The injection head of claim 3 further comprising one or more second protrusions disposed at the second end of the barrel, wherein the second protrusions have a height less than a height of the first protrusions.
  5. 5. The injector head of claim 4, wherein the barrel further comprises one or more separation bars disposed in the second opening dividing the second opening into a plurality of sections, wherein the first protrusion and/or the second protrusion are disposed on the separation bars and extend outwardly of the barrel.
  6. 6. The injection head of claim 4, wherein the first protrusion is disposed at a center of the second opening, and the second protrusion is symmetrically disposed at both sides of the first protrusion.
  7. 7. The injector head of claim 4, wherein all or part of the first projection and/or the second projection is disposed on the annular end face.
  8. 8. The injector head of claim 1, wherein the liquid channel comprises a first portion and a second portion, wherein the second portion tapers in size from the first opening to the second opening.
  9. 9. The injection head according to claim 1, wherein the angle of the first projection end is 50-60 °, preferably 53-56 °.
  10. 10. The injector head of claim 9, wherein the first projection comprises first and second surfaces disposed obliquely, wherein the angle is an included angle between the first and second surfaces.
  11. 11. The injection head of claim 10, wherein the height of the first protrusion is 2-5 times the thickness of the plant leaf.
  12. 12. An injection device for injecting liquid into plant leaves comprising an injection head according to any one of claims 1 to 11.
  13. 13. A method of injecting a liquid into a plant leaf comprising: Drawing liquid through the injection device; The first side of the plant leaf is arranged on the flexible object, the injection head of the injection device pierces the plant leaf from the second side of the plant leaf, and a damage wound is formed on the plant leaf; With the projection of the injector head penetrating into the plant leaf, the plant leaf forms a liquid space between the lesion and the second end of the barrel separate from the outside of the barrel against the injector head, and Pushing the liquid in the main body, and allowing the liquid to enter the plant leaves through the damaged opening.
  14. 14. The method of claim 13, wherein the second side of the plant leaf is a backlit plant leaf backside.
  15. 15. The method of claim 13, further comprising stopping pushing the dye solution when the plant leaves are subjected to a shift of infiltration, or the area of the infiltration spots cannot be enlarged continuously, or the area of the infiltration spots occupies the whole plant leaves.

Description

Injection device for injecting liquid into plant leaves and injection head thereof Technical Field The invention relates to the field of injection devices, in particular to an injection device for injecting liquid into plant leaves and an injection head thereof. Background In the related activities of scientific research, daily research, crop improvement and the like, the property improvement of plants is often required, and the regulation and control of target properties such as plant luminescence, morphological change and the like are realized by introducing exogenous genes into plants. For example, in the gene transient expression technology, a target gene is transferred into a target cell in a relatively short time, and a temporary and efficient expression system is constructed in the cell, so that the target gene obtains transient and high-level expression, and the method is not only suitable for related researches of cell biology such as gene-protein interaction and the like, but also has wide application prospect in related experiments of various crops and horticultural plants. At present, a common way to introduce exogenous genes into plants is to inject the exogenous genes into leaves or other suitable sites of the plants by means of an injection device. The conventional injection device is mainly a common medical injector, a microsampler or a simple device which is simply modified, and has the defects that firstly, the damage to plant tissues in the injection process is large, the normal growth of plants and the exogenous gene introduction effect are easily affected, secondly, the injection quantity is difficult to accurately control, the experimental error is easily caused by too much or too little injection quantity, the research accuracy is easily affected, thirdly, the device operation convenience is poor, operators are extremely easy to be pricked by needles in the injection process, potential safety hazards exist, fourthly, the control of injection time and the selection of injection positions have high requirements on operation skills, and strict examination is put forward on the proficiency of the operators, so that the batch injection operation is not facilitated to be effectively carried out. Disclosure of Invention The invention provides an injection head for injecting liquid into plant leaves, which aims at the technical problems existing in the prior art, and comprises a barrel body, one or more first protrusions and one or more second protrusions, wherein the barrel body comprises a first opening, a second opening and a liquid channel, the first opening is arranged at a first end of the barrel body, the second opening is arranged at a second end of the barrel body, the liquid channel is arranged in the barrel body and communicated with the first opening and the second opening and is used for containing the liquid to pass through, the one or more first protrusions are arranged at the second end of the barrel body and are used for forming a damaged wound on the plant leaves, and the second end of the barrel body is suitable for being contacted with the plant leaves and forming a liquid space separated from the outside of the barrel body between the damaged opening and the second end of the barrel body by the plant leaves. The injection head as described above, wherein the pressure of the liquid space is greater than the pressure of the outside during liquid injection. The injection head as described above, wherein the second end of the barrel comprises an annular end surface adapted to abut the plant leaf when the first protrusion penetrates into the plant leaf, thereby defining a liquid space isolated from the outside. The injection head as described above further comprises one or more second protrusions disposed at the second end of the barrel, wherein the second protrusions have a height less than a height of the first protrusions. The injection head as described above, wherein the barrel further comprises one or more separation strips disposed in the second opening dividing the second opening into a plurality of portions, wherein the first protrusion and/or the second protrusion are disposed on the separation strips and extend outwardly of the barrel. The injection head as described above, wherein the first protrusion is disposed at the center of the second opening, and the second protrusions are symmetrically disposed at both sides of the first protrusion. The injection head as described above, wherein all or part of the first protrusion and/or the second protrusion is provided on the annular end surface. The injection head as described above, wherein the liquid channel comprises a first portion and a second portion, wherein the second portion tapers in size from the first opening to the second opening. The injection head as described above, wherein the angle of the first projection end is 50-60 °, preferably 53-56 ° The injection head as described above, wherein the first protrus