CN-122012227-A - Portable microorganism growth curve analyzer

CN122012227ACN 122012227 ACN122012227 ACN 122012227ACN-122012227-A

Abstract

The invention discloses a portable microorganism growth curve analyzer, and belongs to the technical field of microorganism detection. The analyzer detection module adopts a multi-path selectable wavelength LED light source and photodiode correlation structure, is used for realizing real-time optical density detection of microorganism samples in a 6-pore plate and a 12-pore plate and can automatically draw a growth curve, and the X-axis moving module consists of a slide block structure driven by a stepping motor so as to realize automatic scanning of multichannel samples. The communication module supports Bluetooth and USB interfaces, and can realize control of an upper computer and data export. The invention can realize high-precision, multi-channel and automatic microorganism growth curve detection under a miniaturized structure, has simple and convenient operation, and is suitable for various application scenes such as clinical medicine inspection, medicine research and development, environment monitoring, food safety, water quality detection, agricultural and animal husbandry pathogen monitoring, industrial fermentation and biological processing, public health monitoring, teaching and scientific research and the like.

Inventors

WAN YI
CHEN HONG
DENG YUEMEI
LIN FANGZHU
LIN XIAOTING
Lai Zhuoyuan
GUO TIANCI
Deng Yusi
ZHONG YONGJIE
HUANG ZHENGLIANG
FU ZHAOYONG
Lin Shaolu

Assignees

海南微氪生物科技股份有限公司
海南大学

Dates

Publication Date: 20260512
Application Date: 20260120

Claims (10)

1. The portable microorganism growth curve analyzer is characterized by comprising a body (7), wherein a housing (8) and a fixed base (9) of the body (7) form an external frame of the whole machine, an upper cover plate (10) capable of being opened in a turning mode is hinged with the housing (8) through a damping hinge, and the body (7) comprises: The detection module (1) consists of a plurality of paths of LED light source groups (101) with selectable wavelengths and photodiodes (102) corresponding to the LED light source groups, and forms a correlation light path structure for detecting the optical density of a microorganism sample in an orifice plate in real time; The X-axis moving module (2) consists of a stepping motor (201), a lead screw (202) and a sliding block (203) and is used for driving the detecting module (2) to move along the X-axis direction so as to realize the detection of different hole sites in the porous plate one by one; The pore plate bracket component (3) is arranged in the sample chamber in the fixed base and is used for placing a 6-hole microorganism culture plate or a 12-hole microorganism culture plate; The temperature control module (4) is arranged at the inner side of the upper cover plate (10) and consists of a heating plate (401) and a temperature sensor (402) and is used for maintaining the temperature of the upper cover plate (10) above the ambient temperature so as to inhibit the generation of condensed water and improve the detection precision; The integrated circuit control module (5) comprises a microcontroller MCU, an analog-to-digital conversion module ADC, an LED constant current driving module, a motor driving module and a power input module, and is used for controlling and processing data in the detection process; The communication module (6) comprises a USB interface and a Bluetooth module, wherein the Bluetooth module supports communication with an upper computer and can simultaneously support connection of at most 16 devices, and the upper computer software system is used for setting sampling frequency, temperature control parameters and detection pore plate types, supporting remote control devices and exporting detection data.
2. The portable microorganism growth curve analyzer according to claim 1, wherein the LED light source group (101) supports multi-wavelength output, the detection module (1) comprises 8 LED light sources and 8 photodiodes corresponding to the LED light sources, the LED light sources are driven by a constant current driving chip, and optical signals transmitted through a sample are collected by the photodiodes and converted into electrical signals.
3. The portable microorganism growth curve analyzer according to claim 1, wherein the detection module (1) further comprises a photoelectric detection assembly (103), the photoelectric detection assembly (103) is mounted on an L-shaped detection support (104), the LED light source group (101) and the photodiode (102), namely, the photo sensor group, are respectively mounted at the upper end and the lower end of the L-shaped detection support (104) to form a correlation light path structure, and synchronously move along with a sliding block (203) of the X-axis movement module (2) to realize point-by-point scanning of different hole sites in the hole plate, and the sliding block (203) of the X-axis movement module (2) is driven by a stepping motor (201) and realizes accurate displacement control through transmission of a lead screw (202).
4. A portable microorganism growth curve analyzer according to claim 3, wherein the photoelectric detection assembly (103) comprises a light source plate (105) and a photoelectric signal collection plate (106), the LED light source group (101) is mounted on the light source plate (105), the light source plate (105) is fixed at one end of the stepper motor (201), the light source plate (105) is responsible for providing stable constant current driving, so that the light source group emits 600nm wavelength light to irradiate the sample, the emitted light passes through the sample and is received by a photodiode (102) at the correlation position, the photodiode (102) is a receiver of optical signals, called as a 'photo-sensor group', the photoelectric signal collection plate (106) is a processing center of the optical signals, the photodiode (102) is mounted on the photoelectric signal collection plate (106), the photoelectric signal collection plate (106) is fixed at the other end of the stepper motor (201), and the photoelectric signal collection plate (106) receives and amplifies and converts current signals generated by the photodiode into stable voltage signals through an operation amplifying circuit, a transimpedance amplifier and a high-sensitivity signal conditioning system in the light source plate (106) to realize the linear change of the correlation coefficients of the ADC (99.0).
5. The portable microbial growth curve analyzer according to claim 1, wherein the temperature control module (4) adopts a closed-loop control system to regulate the temperature of the heating plate to be in the range of ambient temperature +4 ℃ to 50 ℃, and the heating plate (401) has double functions of preventing condensed water in the flip cover from generating and maintaining the ambient temperature control, so as to stabilize the ambient temperature to be in the range of 25 ℃ to 40 ℃.
6. The portable microorganism growth curve analyzer according to claim 1, wherein the upper computer software sets sampling time intervals to 1-60 minutes and has functions of automatically generating microorganism growth curves, outputting detection data and upgrading remote firmware, and is connected with the analyzer through a wired serial port or wireless Bluetooth and can set continuous monitoring for 1-100 hours; The curve generation process is as follows: recording scattered light intensity every 1 minute, immediately executing smoothing filter algorithm to eliminate transient high-frequency noise after software receives scattered light intensity data stream every 1 minute to ensure continuity of data sequence, then using filtered discrete points for nonlinear regression fitting, fitting accurately into growth dynamics model, using Logistic growth dynamics model with function form of The system adopts a Levenberg-Marquardt L-M iterative optimization algorithm to determine the maximum value of the light intensity of the model parameter A, Maximum specific growth rate Automatically and dynamically drawing a smooth and accurate microorganism growth curve by a software system by taking time as a horizontal axis and scattered light intensity as a vertical axis through the continuous and accurate fitting function in the hysteresis period, automatically calculating the inhibition rate of each drug concentration by the program by utilizing the maximum light intensity parameter extracted by the fitting model after the curve drawing is completed, And finally, automatically searching the minimum drug concentration meeting the threshold value of the inhibition rate of less than or equal to 90% -100% according to the drug sensitivity test standard, and automatically judging the minimum drug concentration as an MIC value.
7. The portable microorganism growth curve analyzer according to claim 1, wherein the detection accuracy of the detection module (1) is ±1%, the coefficient of variation CV of the consistency test is equal to or less than ±0.5%, the linear correlation coefficient R2 is greater than 0.99, and the temperature control accuracy of the temperature control module is ±1 ℃.
8. The microbial growth curve analyzer according to claim 1, wherein the analyzer automatically completes self-checking and position initialization after being powered on, and reports state information to a host computer, the communication module supports Bluetooth serial port transparent transmission protocol, supports 16 devices connected in parallel to the host computer, and supports power-off reconnection and historical data uploading functions.
9. The microorganism growth curve analyzer according to claim 1, wherein the housing (8) further comprises a plurality of connecting pieces (801), the connecting pieces (801) are connected with the fixed base (9), the fixed base (9) further comprises a plurality of foot pads, the detection module (1) body is fixed on the fixed base (9) through a fastener lock (107), the upper cover plate (10) is provided with a handle, the X-axis moving module (2) comprises two core driving elements, namely a motor and a screw rod, capable of enabling the detection module (1) and the sliding block (203) to move along an X axis, and an infrared position sensor (14) is arranged beside the X axis and connected with a main controller and used for detecting the initial position of the sliding block (203) of the X-axis moving module (2).
10. The microorganism growth curve analyzer according to claim 1, wherein the orifice plate bracket set (3) is provided with an orifice plate bracket (301), an instrument bracket (15) is arranged below the orifice plate bracket (301), a left supporting foot seat (302) and a right supporting foot seat (303) are respectively arranged at the bottoms of two sides of the whole body (7), the left supporting foot seat (302) and the right supporting foot seat (303) are respectively arranged at two sides of the whole body (7), and the X-axis moving module (2) is locked on the orifice plate bracket (301) through a fastener.

Description

Portable microorganism growth curve analyzer Technical Field The invention relates to the technical field of biological detection instruments, in particular to a portable microorganism growth curve analyzer. Background The microorganism has wide application in the fields of environmental monitoring, water quality control, food safety, clinical diagnosis, biological scientific research, industrial fermentation process optimization and the like, and the growth state of the microorganism is often dynamically analyzed through a growth curve. Traditional microorganism growth curve analysis generally depends on microscope observation and manual counting, and the method is tedious, low in efficiency and large in error, and is difficult to meet the requirements of modern high throughput, real-time performance and automation. Although some automatic analysis instruments can realize real-time monitoring and data recording to a certain extent, the problems of high price, huge volume, complex operation and the like exist, and the on-site deployment and the use of basic units are not facilitated. In addition, existing devices still suffer from a number of deficiencies in light source wavelength selection, environmental parameter adjustment, wireless communication, and integration, and lack the ability to adapt to a variety of microorganism types and growth requirements. At present, research in the field of portable microorganism detection equipment is continuously advanced at home and abroad, and particularly, a design scheme with high integration level, multiple functions and multiple parameter fusion is concerned. Under the background, the development of the microorganism growth curve analyzer which has the advantages of compact structure, accurate detection, stable operation, flexible communication and adaptation to different spectrums and environmental conditions becomes a key technical direction for meeting the demands of scientific research, teaching and field detection. Based on the requirements, the invention provides a multifunctional microorganism growth curve analyzer, which integrates modules such as filter wheel control, LED multi-wavelength light source, automatic temperature control, bluetooth communication, data acquisition and analysis, and the like, realizes continuous monitoring and analysis of microorganism growth process, improves detection efficiency and data accuracy, and is suitable for various application scenes such as laboratory and mobile detection. Disclosure of Invention The invention aims to provide a portable detection instrument for analyzing a microorganism growth curve, and aims to solve the problems of low detection efficiency, poor integration level, high cost, complex operation and incapability of realizing dynamic monitoring in the prior art. The detection instrument integrates an optical detection technology, a temperature control technology, a microfluidic technology, a wireless communication technology and an intelligent data processing technology, and achieves high-precision real-time monitoring of the growth state of microorganisms and automatic drawing of dynamic curves. . The invention is realized by the following technical scheme: the portable microorganism growth curve analyzer comprises a body, wherein a housing and a fixed base of the body form an external frame of the whole machine, an openable upper cover plate is hinged with the housing through a damping hinge, and the body comprises: the detection module consists of a plurality of paths of LED light source groups with selectable wavelengths and photodiodes corresponding to the LED light source groups, and forms a correlation light path structure which is used for detecting the optical density of a microorganism sample in the pore plate in real time; the X-axis moving module consists of a stepping motor, a screw rod and a sliding block and is used for driving the detecting module to move along the X-axis direction so as to realize the detection of different hole sites in the porous plate one by one; The pore plate bracket component is arranged in the sample chamber in the fixed base and is used for placing a 6-hole microorganism culture plate or a 12-hole microorganism culture plate; The temperature control module is arranged at the inner side of the upper cover plate and consists of a heating plate and a temperature sensor, and is used for maintaining the temperature of the upper cover plate above the ambient temperature so as to inhibit condensed water from generating and improve the detection precision; the integrated circuit control module comprises a microcontroller MCU, an analog-to-digital conversion module ADC, an LED constant current driving module, a motor driving module and a power input module, and is used for controlling and processing data in the detection process; The communication module comprises a USB interface and a Bluetooth module, wherein the Bluetooth module supports communication with an upper